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SLC6A11 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  SLC6A11
Gene Name:  solute carrier family 6 (neurotransmitter transporter), member 11
Sequence             
Original Clone ID: ccsbBroad304_11136
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00437137

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 625nts         Open reading frame : 1 to 625
Reference Sequence Alignment

ATGACGGCGGAGAAGGCGCTGCCCCTGGGCAATGGGAAGGCTGCTGAGGAGGCGCGGGAG
TCCGAGGCGCCGGGTGGCGGCTGCAGCAGCGGGGGCGCGGCGCCCGCGCGCCACCCGCGC
GTCAAGCGCGACAAGGCGGTCCACGAGCGCGGCCACTGGAACAACAAGGTGGAGTTCGTG
CTGAGCGTGGCCGGGGAGATCATTGGGCTGGGCAACGTGTGGCGCTTCCCCTACCTGTGC
TACAAGAACGGAGGAGGGGCATTCCTGATTCCCTACGTGGTGTTTTTTATTTGCTGTGGA
ATTCCTGTTTTTTTCCTGGAGACAGCTCTGGGGCAGTTCACAAGTGAAGGTGGCATTACG
TGTTGGAGGAAAGTTTGCCCTTTATTTGAAGGCATTGGCTATGCAACACAGGTGATTGAG
GCCCATCTGAATGTGTACTACATCATCATCCTGGCATGGGCCATTTTTTACCTGAGCAAC
TGCTTCACTACTGAGCTACCCTGGGCTACCTGTGGGCATGAGTGGAACACAGAGAATTGT
GTGGAGTTCCAGAAACTGAATGTGAGCAACTACAGCCATGTGTCTCTGCAGAATGCCACC
TCCCCTGTCATGGAGTTTTGGGAG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : SLC6A11
Symbol Nomenclature : SLC6A11
SYNONYM : GAT-3; GAT3; GAT4
Designation : GABA transporter 3|sodium- and chloride-dependent GABA transporter 3|solute carrier family 6 (neurotransmitter transporter, GABA), member 11|solute carrier family 6 member 11
Full Nomenclature : solute carrier family 6 (neurotransmitter transporter), member 11
GENEID : 6538
GenBank Accession : BC152735
HGNC : 11044
MIM : 607952
Target GenBank: BC152735

 

Reference Sequence Alignment

  

HsCD00437137      ATGACGGCGGAGAAGGCGCTGCCCCTGGGCAATGGGAAGGCTGCTGAGGAGGCGCGGGAG
NM_014229.1       ATGACGGCGGAGAAGGCGCTGCCCCTGGGCAATGGGAAGGCTGCTGAGGAGGCGCGGGAG
                  ************************************************************

HsCD00437137      TCCGAGGCGCCGGGTGGCGGCTGCAGCAGCGGGGGCGCGGCGCCCGCGCGCCACCCGCGC
NM_014229.1       TCCGAGGCGCCGGGTGGCGGCTGCAGCAGCGGGGGCGCGGCGCCCGCGCGCCACCCGCGC
                  ************************************************************

HsCD00437137      GTCAAGCGCGACAAGGCGGTCCACGAGCGCGGCCACTGGAACAACAAGGTGGAGTTCGTG
NM_014229.1       GTCAAGCGCGACAAGGCGGTCCACGAGCGCGGCCACTGGAACAACAAGGTGGAGTTCGTG
                  ************************************************************

HsCD00437137      CTGAGCGTGGCCGGGGAGATCATTGGGCTGGGCAACGTGTGGCGCTTCCCCTACCTGTGC
NM_014229.1       CTGAGCGTGGCCGGGGAGATCATTGGGCTGGGCAACGTGTGGCGCTTCCCCTACCTGTGC
                  ************************************************************

HsCD00437137      TACAAGAACGGAGGAGGGGCATTCCTGATTCCCTACGTGGTGTTTTTTATTTGCTGTGGA
NM_014229.1       TACAAGAACGGAGGAGGGGCATTCCTGATTCCCTACGTGGTGTTTTTTATTTGCTGTGGA
                  ************************************************************

HsCD00437137      ATTCCTGTTTTTTTCCTGGAGACAGCTCTGGGGCAGTTCACAAGTGAAGGTGGCATTACG
NM_014229.1       ATTCCTGTTTTTTTCCTGGAGACAGCTCTGGGGCAGTTCACAAGTGAAGGTGGCATTACG
                  ************************************************************

HsCD00437137      TGTTGGAGGAAAGTTTGCCCTTTATTTGAAGGCATTGGCTATGCAACACAGGTGATTGAG
NM_014229.1       TGTTGGAGGAAAGTTTGCCCTTTATTTGAAGGCATTGGCTATGCAACACAGGTGATTGAG
                  ************************************************************

HsCD00437137      GCCCATCTGAATGTGTACTACATCATCATCCTGGCATGGGCCATTTTTTACCTGAGCAAC
NM_014229.1       GCCCATCTGAATGTGTACTACATCATCATCCTGGCATGGGCCATTTTTTACCTGAGCAAC
                  ************************************************************

HsCD00437137      TGCTTCACTACTGAGCTACCCTGGGCTACCTGTGGGCATGAGTGGAACACAGAGAATTGT
NM_014229.1       TGCTTCACTACTGAGCTACCCTGGGCTACCTGTGGGCATGAGTGGAACACAGAGAATTGT
                  ************************************************************

HsCD00437137      GTGGAGTTCCAGAAACTGAATGTGAGCAACTACAGCCATGTGTCTCTGCAGAATGCCACC
NM_014229.1       GTGGAGTTCCAGAAACTGAATGTGAGCAACTACAGCCATGTGTCTCTGCAGAATGCCACC
                  ************************************************************

HsCD00437137      TCCCCTGTCATGGAGTTTTGGGAG------------------------------------
NM_014229.1       TCCCCTGTCATGGAGTTTTGGGAGCACCGGGTCCTGGCCATCTCTGACGGGATCGAGCAC
                  ************************                                    

HsCD00437137      ------------------------------------------------------------
NM_014229.1       ATCGGGAACCTTCGCTGGGAGCTGGCCTTGTGTCTCTTGGCAGCCTGGACCATCTGTTAC
                                                                              

HsCD00437137      ------------------------------------------------------------
NM_014229.1       TTCTGTATCTGGAAGGGGACCAAGTCTACAGGAAAGGTTGTATACGTGACTGCGACATTC
                                                                              

HsCD00437137      ------------------------------------------------------------
NM_014229.1       CCCTACATCATGCTGCTGATCCTCCTGATACGAGGGGTCACGTTGCCCGGGGCCTCAGAG
                                                                              

HsCD00437137      ------------------------------------------------------------
NM_014229.1       GGCATCAAGTTCTACTTGTACCCTGACCTCTCCCGGCTCTCCGACCCCCAGGTCTGGGTA
                                                                              

HsCD00437137      ------------------------------------------------------------
NM_014229.1       GATGCTGGAACGCAGATCTTTTTCTCCTATGCCATTTGCCTGGGCTGTCTGACCGCTCTG
                                                                              

HsCD00437137      ------------------------------------------------------------
NM_014229.1       GGAAGTTATAACAATTATAACAACAACTGCTACAGGGACTGCATCATGCTCTGTTGCCTG
                                                                              

HsCD00437137      ------------------------------------------------------------
NM_014229.1       AACAGCGGCACCAGCTTCGTGGCTGGGTTTGCCATCTTCTCAGTCCTGGGTTTTATGGCG
                                                                              

HsCD00437137      ------------------------------------------------------------
NM_014229.1       TACGAGCAGGGGGTACCCATTGCTGAGGTGGCAGAGTCAGGCCCCGGCCTGGCCTTTATT
                                                                              

HsCD00437137      ------------------------------------------------------------
NM_014229.1       GCGTACCCCAAGGCGGTCACCATGATGCCTCTCTCCCCGCTGTGGGCCACCTTGTTCTTC
                                                                              

HsCD00437137      ------------------------------------------------------------
NM_014229.1       ATGATGCTCATCTTCCTGGGCCTGGACAGCCAGTTTGTGTGTGTGGAAAGCCTGGTGACC
                                                                              

HsCD00437137      ------------------------------------------------------------
NM_014229.1       GCCGTGGTGGACATGTACCCCAAGGTTTTCCGGAGGGGTTACCGGCGGGAGCTGCTCATC
                                                                              

HsCD00437137      ------------------------------------------------------------
NM_014229.1       CTAGCCTTGTCTGTTATCTCCTATTTTCTGGGCCTCGTGATGTTAACAGAGGGTGGCATG
                                                                              

HsCD00437137      ------------------------------------------------------------
NM_014229.1       TACATCTTCCAGCTCTTTGACTCCTATGCCGCCAGTGGGATGTGCCTTCTCTTCGTGGCC
                                                                              

HsCD00437137      ------------------------------------------------------------
NM_014229.1       ATCTTTGAGTGCATCTGCATCGGCTGGGTGTATGGAAGCAACCGGTTCTATGATAACATT
                                                                              

HsCD00437137      ------------------------------------------------------------
NM_014229.1       GAAGACATGATTGGCTACCGGCCACCGTCGCTCATTAAGTGGTGCTGGATGATCATGACC
                                                                              

HsCD00437137      ------------------------------------------------------------
NM_014229.1       CCTGGGATCTGCGCGGGGATCTTCATCTTCTTCTTGATCAAGTACAAGCCACTCAAGTAC
                                                                              

HsCD00437137      ------------------------------------------------------------
NM_014229.1       AACAACATCTACACCTACCCAGCCTGGGGCTATGGCATTGGCTGGCTCATGGCCCTGTCC
                                                                              

HsCD00437137      ------------------------------------------------------------
NM_014229.1       TCCATGCTCTGCATCCCGCTCTGGATCTGCATCACAGTGTGGAAGACGGAGGGGACACTG
                                                                              

HsCD00437137      ------------------------------------------------------------
NM_014229.1       CCCGAGAAACTCCAGAAGTTGACGACCCCCAGCACAGATCTGAAAATGCGGGGCAAGCTT
                                                                              

HsCD00437137      ------------------------------------------------------------
NM_014229.1       GGGGTGAGCCCACGGATGGTGACAGTTAATGACTGTGATGCCAAACTCAAGAGTGACGGG
                                                                              

HsCD00437137      ---------------------------------------
NM_014229.1       ACCATCGCAGCCATCACAGAGAAGGAGACGCACTTCTGA
                                                         

Pathways

Reactome : Amine compound SLC transporters
Reactome : Creatine metabolism
Reactome : GABA synthesis, release, reuptake and degradation
Reactome : Metabolism
Reactome : Metabolism of amino acids and derivatives
Reactome : Na+/Cl- dependent neurotransmitter transporters
Reactome : Neuronal System
Reactome : Neurotransmitter Release Cycle
Reactome : Reuptake of GABA
Reactome : SLC-mediated transmembrane transport
Reactome : Transmembrane transport of small molecules
Reactome : Transmission across Chemical Synapses
Reactome : Transport of glucose and other sugars, bile salts and organic acids, metal ions and amine compounds

Protein

HPRD : 06406

Clone

Original Clone ID : ccsbBroad304_11136

Organism


TAX_ID : 9606
Species Specific ID: 6538

Genome

Chromosome : 3
Map Location : 3p25.3

Reagents

Labome : GAT-3-antibody