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CDC34 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  CDC34
Gene Name:  cell division cycle 34
Sequence             
Original Clone ID: ccsbBroad304_00274
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00437232

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 709nts         Open reading frame : 1 to 709
Reference Sequence Alignment

ATGGCTCGGCCGCTAGTGCCCAGCTCGCAGAAGGCGCTGCTGCTGGAGCTCAAGGGGCTG
CAGGAAGAGCCGGTCGAGGGATTCCGCGTGACACTGGTGGACGAGGGCGATCTATACAAC
TGGGAGGTGGCCATCTTCGGGCCCCCCAACACCTACTACGAGGGCGGCTACTTCAAGGCG
CGCCTCAAGTTCCCCATCGACTACCCATACTCTCCACCAGCCTTTCGGTTCCTGACCAAG
ATGTGGCACCCTAACATCTACGAGACGGGGGACGTGTGTATCTCCATCCTCCACCCGCCG
GTGGACGACCCCCAGAGCGGGGAGCTGCCCTCAGAGAGGTGGAACCCCACGCAGAACGTC
AGGACCATTCTCCTGAGTGTGATCTCCCTCCTGAACGAGCCCAACACCTTCTCGCCCGCA
AACGTGGACGCCTCCGTGATGTACAGGAAGTGGAAAGAGAGCAAGGGGAAGGATCGGGAG
TACACAGACATCATCCGGAAGCAGGTCCTGGGGACCAAGGTGGACGCGGAGCGTGACGGC
GTGAAGGTGCCCACCACGCTGGCCGAGTACTGCGTGAAGACCAAGGCGCCGGCGCCCGAC
GAGGGCTCAGACCTCTTCTACGACGACTACTACGAGGACGGCGAGGTGGAGGAGGAGGCC
GACAGCTGCTTCGGGGACGATGAGGATGACTCTGGCACGGAGGAGTCC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CDC34
Symbol Nomenclature : CDC34
SYNONYM : E2-CDC34; UBC3; UBCH3; UBE2R1
Designation : cell division cycle 34 homolog|ubiquitin carrier protein|ubiquitin-conjugating enzyme E2 R1|ubiquitin-conjugating enzyme E2-32 KDA complementing|ubiquitin-conjugating enzyme E2-CDC34|ubiquitin-protein ligase R1
Full Nomenclature : cell division cycle 34
GENEID : 997
HGNC : 1734
MIM : 116948
Vega : OTTHUMG00000180558
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00437232      ATGGCTCGGCCGCTAGTGCCCAGCTCGCAGAAGGCGCTGCTGCTGGAGCTCAAGGGGCTG
NM_004359.1       ATGGCTCGGCCGCTAGTGCCCAGCTCGCAGAAGGCGCTGCTGCTGGAGCTCAAGGGGCTG
                  ************************************************************

HsCD00437232      CAGGAAGAGCCGGTCGAGGGATTCCGCGTGACACTGGTGGACGAGGGCGATCTATACAAC
NM_004359.1       CAGGAAGAGCCGGTCGAGGGATTCCGCGTGACACTGGTGGACGAGGGCGATCTATACAAC
                  ************************************************************

HsCD00437232      TGGGAGGTGGCCATCTTCGGGCCCCCCAACACCTACTACGAGGGCGGCTACTTCAAGGCG
NM_004359.1       TGGGAGGTGGCCATCTTCGGGCCCCCCAACACCTACTACGAGGGCGGCTACTTCAAGGCG
                  ************************************************************

HsCD00437232      CGCCTCAAGTTCCCCATCGACTACCCATACTCTCCACCAGCCTTTCGGTTCCTGACCAAG
NM_004359.1       CGCCTCAAGTTCCCCATCGACTACCCATACTCTCCACCAGCCTTTCGGTTCCTGACCAAG
                  ************************************************************

HsCD00437232      ATGTGGCACCCTAACATCTACGAGACGGGGGACGTGTGTATCTCCATCCTCCACCCGCCG
NM_004359.1       ATGTGGCACCCTAACATCTACGAGACGGGGGACGTGTGTATCTCCATCCTCCACCCGCCG
                  ************************************************************

HsCD00437232      GTGGACGACCCCCAGAGCGGGGAGCTGCCCTCAGAGAGGTGGAACCCCACGCAGAACGTC
NM_004359.1       GTGGACGACCCCCAGAGCGGGGAGCTGCCCTCAGAGAGGTGGAACCCCACGCAGAACGTC
                  ************************************************************

HsCD00437232      AGGACCATTCTCCTGAGTGTGATCTCCCTCCTGAACGAGCCCAACACCTTCTCGCCCGCA
NM_004359.1       AGGACCATTCTCCTGAGTGTGATCTCCCTCCTGAACGAGCCCAACACCTTCTCGCCCGCA
                  ************************************************************

HsCD00437232      AACGTGGACGCCTCCGTGATGTACAGGAAGTGGAAAGAGAGCAAGGGGAAGGATCGGGAG
NM_004359.1       AACGTGGACGCCTCCGTGATGTACAGGAAGTGGAAAGAGAGCAAGGGGAAGGATCGGGAG
                  ************************************************************

HsCD00437232      TACACAGACATCATCCGGAAGCAGGTCCTGGGGACCAAGGTGGACGCGGAGCGTGACGGC
NM_004359.1       TACACAGACATCATCCGGAAGCAGGTCCTGGGGACCAAGGTGGACGCGGAGCGTGACGGC
                  ************************************************************

HsCD00437232      GTGAAGGTGCCCACCACGCTGGCCGAGTACTGCGTGAAGACCAAGGCGCCGGCGCCCGAC
NM_004359.1       GTGAAGGTGCCCACCACGCTGGCCGAGTACTGCGTGAAGACCAAGGCGCCGGCGCCCGAC
                  ************************************************************

HsCD00437232      GAGGGCTCAGACCTCTTCTACGACGACTACTACGAGGACGGCGAGGTGGAGGAGGAGGCC
NM_004359.1       GAGGGCTCAGACCTCTTCTACGACGACTACTACGAGGACGGCGAGGTGGAGGAGGAGGCC
                  ************************************************************

HsCD00437232      GACAGCTGCTTCGGGGACGATGAGGATGACTCTGGCACGGAGGAGTCC---
NM_004359.1       GACAGCTGCTTCGGGGACGATGAGGATGACTCTGGCACGGAGGAGTCCTGA
                  ************************************************   

Pathways

Reactome : Adaptive Immune System
Reactome : Antigen processing: Ubiquitination & Proteasome degradation
Reactome : Class I MHC mediated antigen processing & presentation
Reactome : Immune System

Clone

Original Clone ID : ccsbBroad304_00274

Organism


TAX_ID : 9606
Species Specific ID: 997

Genome

Chromosome : 19
Map Location : 19p13.3
Ensembl : ENSG00000099804

Reagents

Labome : CDC34-antibody