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ACTR1A (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ACTR1B
Gene Name:  ARP1 actin-related protein 1 homolog B, centractin beta (yeast)
Sequence             
Original Clone ID: ccsbBroad304_02320
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00437435

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1129nts         Open reading frame : 1 to 1129
Reference Sequence Alignment

ATGGAGTCCTACGATGTGATCGCCAACCAGCCTGTCGTGATCGACAACGGATCCGGTGTG
ATTAAAGCTGGTTTTGCTGGTGATCAGATCCCCAAATACTGCTTTCCAAACTATGTGGGC
CGACCCAAGCACGTTCGTGTCATGGCAGGAGCCCTTGAAGGCGACATCTTCATTGGCCCC
AAAGCTGAGGAGCACCGAGGGCTGCTTTCAATCCGCTATCCCATGGAGCATGGCATCGTC
AAGGATTGGAACGACATGGAACGCATTTGGCAATATGTCTATTCTAAGGACCAGCTGCAG
ACTTTCTCAGAGGAGCATCCTGTGCTCCTGACTGAGGCGCCTTTAAACCCACGAAAAAAC
CGGGAACGAGCTGCCGAAGTTTTCTTCGAGACCTTCAATGTGCCCGCTCTTTTCATCTCC
ATGCAAGCTGTACTCAGCCTTTACGCTACAGGCAGGACCACAGGGGTGGTGCTGGATTCT
GGGGATGGAGTCACCCATGCTGTGCCCATCTATGAGGGCTTTGCCATGCCCCACTCCATC
ATGCGCATCGACATCGCGGGCCGGGACGTCTCTCGCTTCCTGCGCCTCTACCTGCGTAAG
GAGGGCTACGACTTCCACTCATCCTCTGAGTTTGAGATTGTCAAGGCCATAAAAGAAAGA
GCCTGTTACCTATCCATAAACCCCCAAAAGGATGAGACGCTAGAGACAGAGAAAGCTCAG
TACTACCTGCCTGATGGCAGCACCATTGAGATTGGTCCTTCCCGATTCCGGGCCCCTGAG
TTGCTCTTCAGGCCAGATTTGATTGGAGAGGAGAGTGAAGGCATCCACGAGGTCCTGGTG
TTCGCCATTCAGAAGTCAGACATGGACCTGCGGCGCACGCTTTTCTCTAACATTGTCCTC
TCAGGAGGCTCTACCCTGTTCAAAGGTTTTGGTGACAGGCTCCTGAGTGAAGTGAAGAAA
CTAGCTCCAAAAGATGTGAAGATCAGGATATCTGCACCTCAGGAGAGACTGTATTCCACG
TGGATTGGGGGCTCCATCCTTGCCTCCCTGGACACCTTTAAGAAGATGTGGGTCTCCAAA
AAGGAATATGAGGAAGACGGTGCCCGATCCATCCACAGAAAAACCTTC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: ''
Sequencing Primer: Forward:  CMV forward
Reverse:  None
Description: with tag/fusion/marker assay, Gateway®, acceptor (destination), recombinational cloning clone, lentiviral infection expression with BlastR and T7 and attR1 and CMV and cPPT and V5 and AmpR and attR2 and CAT and ccdB and WPRE; ampicillin resistance in bacterial, blasticidin resistance in mammalian;
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: EvNO00877063
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter CMV CMV promoter 14 566
promoter T7 T7 promoter 3690 3672
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker BlastR Blasticidin resistance gene 8753 9148
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ACTR1B
Symbol Nomenclature : ACTR1B
SYNONYM : ARP1B; CTRN2; PC3
Designation : actin-related protein 1B|beta-centractin|centractin beta
Full Nomenclature : ARP1 actin-related protein 1 homolog B, centractin beta (yeast)
GENEID : 10120
GenBank Accession : DQ895934
HGNC : 168
MIM : 605144
Vega : OTTHUMG00000130549
Target GenBank: DQ895934

 

Reference Sequence Alignment

  

HsCD00437435      ATGGAGTCCTACGATGTGATCGCCAACCAGCCTGTCGTGATCGACAACGGATCCGGTGTG
NM_005735.3       ATGGAGTCCTACGACATCATCGCCAACCAGCCTGTGGTCATCGACAACGGTTCGGGGGTG
NM_005736.3       ATGGAGTCCTACGATGTGATCGCCAACCAGCCTGTCGTGATCGACAACGGATCCGGTGTG
                  ************** .* ***************** ** ***********:** ** ***

HsCD00437435      ATTAAAGCTGGTTTTGCTGGTGATCAGATCCCCAAATACTGCTTTCCAAACTATGTGGGC
NM_005735.3       ATTAAAGCTGGCTTTGCAGGAGACCAGATTCCCAAATACTGTTTCCCAAACTATGTCGGG
NM_005736.3       ATTAAAGCTGGTTTTGCTGGTGATCAGATCCCCAAATACTGCTTTCCAAACTATGTGGGC
                  *********** *****:**:** ***** *********** ** *********** ** 

HsCD00437435      CGACCCAAGCACGTTCGTGTCATGGCAGGAGCCCTTGAAGGCGACATCTTCATTGGCCCC
NM_005735.3       CGGCCGAAGCACATGCGGGTGATGGCTGGAGCCCTGGAGGGGGACCTCTTCATCGGACCA
NM_005736.3       CGACCCAAGCACGTTCGTGTCATGGCAGGAGCCCTTGAAGGCGACATCTTCATTGGCCCC
                  **.** ******.* ** ** *****:******** **.** ***.******* **.**.

HsCD00437435      AAAGCTGAGGAGCACCGAGGGCTGCTTTCAATCCGCTATCCCATGGAGCATGGCATCGTC
NM_005735.3       AAAGCAGAGGAGCACCGGGGGCTGCTGACCATCCGCTACCCCATGGAGCACGGCGTGGTG
NM_005736.3       AAAGCTGAGGAGCACCGAGGGCTGCTTTCAATCCGCTATCCCATGGAGCATGGCATCGTC
                  *****:***********.******** :*.******** *********** ***.* ** 

HsCD00437435      AAGGATTGGAACGACATGGAACGCATTTGGCAATATGTCTATTCTAAGGACCAGCTGCAG
NM_005735.3       CGAGACTGGAACGACATGGAACGCATCTGGCAGTACGTCTACTCCAAGGATCAGCTGCAG
NM_005736.3       AAGGATTGGAACGACATGGAACGCATTTGGCAATATGTCTATTCTAAGGACCAGCTGCAG
                  ...** ******************** *****.** ***** ** ***** *********

HsCD00437435      ACTTTCTCAGAGGAGCATCCTGTGCTCCTGACTGAGGCGCCTTTAAACCCACGAAAAAAC
NM_005735.3       ACCTTCTCGGAGGAGCATCCTGTGCTCCTCACGGAGGCCCCGCTCAACCCGAGTAAGAAC
NM_005736.3       ACTTTCTCAGAGGAGCATCCTGTGCTCCTGACTGAGGCGCCTTTAAACCCACGAAAAAAC
                  ** *****.******************** ** ***** **  *.*****..*:**.***

HsCD00437435      CGGGAACGAGCTGCCGAAGTTTTCTTCGAGACCTTCAATGTGCCCGCTCTTTTCATCTCC
NM_005735.3       CGGGAGAAGGCGGCAGAGGTGTTCTTTGAGACCTTCAACGTGCCGGCCCTGTTCATCTCC
NM_005736.3       CGGGAACGAGCTGCCGAAGTTTTCTTCGAGACCTTCAATGTGCCCGCTCTTTTCATCTCC
                  *****....** **.**.** ***** *********** ***** ** ** *********

HsCD00437435      ATGCAAGCTGTACTCAGCCTTTACGCTACAGGCAGGACCACAGGGGTGGTGCTGGATTCT
NM_005735.3       ATGCAGGCTGTGCTCAGTCTGTACGCAACAGGACGCACGACAGGAGTGGTTCTAGACTCA
NM_005736.3       ATGCAAGCTGTACTCAGCCTTTACGCTACAGGCAGGACCACAGGGGTGGTGCTGGATTCT
                  *****.*****.***** ** *****:*****..* ** *****.***** **.** **:

HsCD00437435      GGGGATGGAGTCACCCATGCTGTGCCCATCTATGAGGGCTTTGCCATGCCCCACTCCATC
NM_005735.3       GGGGACGGGGTCACTCATGCTGTGCCCATCTATGAGGGCTTTGCCATGCCTCACTCCATC
NM_005736.3       GGGGATGGAGTCACCCATGCTGTGCCCATCTATGAGGGCTTTGCCATGCCCCACTCCATC
                  ***** **.***** *********************************** *********

HsCD00437435      ATGCGCATCGACATCGCGGGCCGGGACGTCTCTCGCTTCCTGCGCCTCTACCTGCGTAAG
NM_005735.3       ATGCGGGTGGACATTGCCGGCCGCGACGTCTCCCGCTACCTCCGACTCCTGCTGCGCAAG
NM_005736.3       ATGCGCATCGACATCGCGGGCCGGGACGTCTCTCGCTTCCTGCGCCTCTACCTGCGTAAG
                  ***** .* ***** ** ***** ******** ****:*** **.*** : ***** ***

HsCD00437435      GAGGGCTACGACTTCCACTCATCCTCTGAGTTTGAGATTGTCAAGGCCATAAAAGAAAGA
NM_005735.3       GAAGGGGTTGACTTCCATACCTCGGCTGAGTTTGAGGTTGTCCGGACAATCAAAGAGCGA
NM_005736.3       GAGGGCTACGACTTCCACTCATCCTCTGAGTTTGAGATTGTCAAGGCCATAAAAGAAAGA
                  **.**  : ******** :*.**  ***********.*****..*.*.**.*****..**

HsCD00437435      GCCTGTTACCTATCCATAAACCCCCAAAAGGATGAGACGCTAGAGACAGAGAAAGCTCAG
NM_005735.3       GCGTGCTACCTGTCCATCAACCCACAGAAGGATGAGGCTCTGGAGACGGAGAAGGTGCAG
NM_005736.3       GCCTGTTACCTATCCATAAACCCCCAAAAGGATGAGACGCTAGAGACAGAGAAAGCTCAG
                  ** ** *****.*****.*****.**.*********.* **.*****.*****.*  ***

HsCD00437435      TACTACCTGCCTGATGGCAGCACCATTGAGATTGGTCCTTCCCGATTCCGGGCCCCTGAG
NM_005735.3       TACACGTTGCCAGACGGCAGCACGCTTGATGTGGGGCCTGCACGATTCCGGGCCCCCGAG
NM_005736.3       TACTACCTGCCTGATGGCAGCACCATTGAGATTGGTCCTTCCCGATTCCGGGCCCCTGAG
                  ***:.  ****:** ******** .**** .* ** *** *.************** ***

HsCD00437435      TTGCTCTTCAGGCCAGATTTGATTGGAGAGGAGAGTGAAGGCATCCACGAGGTCCTGGTG
NM_005735.3       CTGCTGTTCCAGCCGGACCTTGTCGGGGATGAGAGTGAGGGGCTCCATGAGGTGGTGGCC
NM_005736.3       TTGCTCTTCAGGCCAGATTTGATTGGAGAGGAGAGTGAAGGCATCCACGAGGTCCTGGTG
                   **** ***..***.**  * .* **.** ********.** .**** *****  ***  

HsCD00437435      TTCGCCATTCAGAAGTCAGACATGGACCTGCGGCGCACGCTTTTCTCTAACATTGTCCTC
NM_005735.3       TTCGCCATACACAAGTCCGACATGGACCTGCGCCGGACGCTGTTCGCCAACATCGTGCTC
NM_005736.3       TTCGCCATTCAGAAGTCAGACATGGACCTGCGGCGCACGCTTTTCTCTAACATTGTCCTC
                  ********:** *****.************** ** ***** *** * ***** ** ***

HsCD00437435      TCAGGAGGCTCTACCCTGTTCAAAGGTTTTGGTGACAGGCTCCTGAGTGAAGTGAAGAAA
NM_005735.3       TCAGGTGGCTCAACGCTTTTCAAAGGCTTCGGAGACCGATTACTCAGTGAAGTGAAGAAG
NM_005736.3       TCAGGAGGCTCTACCCTGTTCAAAGGTTTTGGTGACAGGCTCCTGAGTGAAGTGAAGAAA
                  *****:*****:** ** ******** ** **:***.*. *.** **************.

HsCD00437435      CTAGCTCCAAAAGATGTGAAGATCAGGATATCTGCACCTCAGGAGAGACTGTATTCCACG
NM_005735.3       CTTGCCCCAAAGGATATCAAAATCAAGATCTCAGCCCCGCAGGAACGGCTGTACTCCACA
NM_005736.3       CTAGCTCCAAAAGATGTGAAGATCAGGATATCTGCACCTCAGGAGAGACTGTATTCCACG
                  **:** *****.***.* **.****.***.**:**.** *****..*.***** *****.

HsCD00437435      TGGATTGGGGGCTCCATCCTTGCCTCCCTGGACACCTTTAAGAAGATGTGGGTCTCCAAA
NM_005735.3       TGGATTGGCGGCTCCATCCTGGCCTCGCTGGACACTTTTAAGAAGATGTGGGTGTCCAAA
NM_005736.3       TGGATTGGGGGCTCCATCCTTGCCTCCCTGGACACCTTTAAGAAGATGTGGGTCTCCAAA
                  ******** *********** ***** ******** ***************** ******

HsCD00437435      AAGGAATATGAGGAAGACGGTGCCCGATCCATCCACAGAAAAACCTTC---
NM_005735.3       AAGGAGTATGAAGAGGATGGCTCCCGTGCTATTCATCGCAAAACTTTCTAG
NM_005736.3       AAGGAATATGAGGAAGACGGTGCCCGATCCATCCACAGAAAAACCTTCTAA
                  *****.*****.**.** **  ****: * ** ** .*.***** ***   

Pathways

Panther : Nicotinic acetylcholine receptor signaling pathway
Reactome : Adaptive Immune System
Reactome : Cell Cycle
Reactome : Cell Cycle, Mitotic
Reactome : Centrosome maturation
Reactome : G2/M Transition
Reactome : Immune System
Reactome : Loss of Nlp from mitotic centrosomes
Reactome : Loss of proteins required for interphase microtubule organizationÿ from the centrosome
Reactome : MHC class II antigen presentation
Reactome : Mitotic G2-G2/M phases
Reactome : Recruitment of mitotic centrosome proteins and complexes
Reactome : Regulation of PLK1 Activity at G2/M Transition

Protein

SMART domain : ACTIN : Actin
HPRD : 05508

Clone

Original Clone ID : ccsbBroad304_02320

Organism


TAX_ID : 9606
Species Specific ID: 10121

Genome

Chromosome : 2
Map Location : 2q11.1-q11.2
Ensembl : ENSG00000115073

Reagents

Labome : ACTR1A-antibody