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ACTR1B (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ACTR1B
Gene Name:  ARP1 actin-related protein 1 homolog B, centractin beta (yeast)
Sequence             
Original Clone ID: ccsbBroad304_07554
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00437443

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1129nts        
Open reading frame : 1 to 1129
Reference Sequence Alignment

ATGGAGTCCTACGACATCATCGCCAACCAGCCTGTGGTCATCGACAACGGTTCGGGGGTG
ATTAAAGCTGGCTTTGCAGGAGACCAGATTCCCAAATACTGTTTCCCAAACTATGTCGGG
CGGCCGAAGCACATGCGGGTGATGGCTGGAGCCCTGGAGGGGGACCTCTTCATCGGACCA
AAAGCAGAGGAGCACCGGGGGCTGCTGACCATCCGCTACCCCATGGAGCACGGCGTGGTG
CGAGACTGGAACGACATGGAACGCATCTGGCAGTACGCCTACTCCAAGGATCAGCTGCAG
ACCTTCTCGGAGGAGCATCCTGTGCTCCTCACGGAGGCCCCGCTCAACCCGAGTAAGAAC
CGGGAGAAGGCGGCAGAGGTGTTCTTTGAGACCTTCAACGTGCCGGCCCTGTTCATCTCC
ATGCAGGCTGTGCTCAGTCTGTACGCAACAGGACGCACGACAGGAGTGGTTCTAGACTCA
GGGGACGGGGTCACTCATGCTGTGCCCATCTATGAGGGCTTTGCCATGCCTCACTCCATC
ATGCGGGTGGACATTGCCGGCCGCGACGTCTCCCGCTACCTCCGACTCCTGCTGCGCAAG
GAAGGGGTTGACTTCCATACCTCGGCTGAGTTTGAGGTTGTCCGGACAATCAAAGAGCGA
GCGTGCTACCTGTCCATCAACCCACAGAAGGATGAGGCTCTGGAGACGGAGAAGGTGCAG
TACACGTTGCCAGACGGCAGCACGCTTGATGTGGGGCCTGCACGATTCCGGGCCCCCGAG
CTGCTGTTCCAGCCGGACCTTGTGGGGGATGAGAGTGAGGGGCTCCATGAGGTGGTGGCC
TTCGCCATACACAAGTCCGACATGGACCTGCGCCGGACGCTGTTCGCCAACATCGTGCTC
TCAGGTGGCTCAACGCTTTTCAAAGGCTTCGGAGACCGATTACTCAGTGAAGTGAAGAAG
CTTGCCCCAAAGGATATCAAAATCAAGATCTCAGCCCCGCAGGAACGGCTGTACTCCACA
TGGATTGGCGGCTCCATCCTGGCCTCGCTGGACACTTTTAAGAAGATGTGGGTGTCCAAA
AAGGAGTATGAAGAGGATGGCTCCCGTGCTATTCATCGCAAAACTTTC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: ''
Sequencing Primer: Forward:  CMV forward
Reverse:  None
Description: with tag/fusion/marker assay, Gateway®, acceptor (destination), recombinational cloning clone, lentiviral infection expression with BlastR and T7 and attR1 and CMV and cPPT and V5 and AmpR and attR2 and CAT and ccdB and WPRE; ampicillin resistance in bacterial, blasticidin resistance in mammalian;
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: EvNO00877063
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter CMV CMV promoter 14 566
promoter T7 T7 promoter 3690 3672
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker BlastR Blasticidin resistance gene 8753 9148
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ACTR1B
Symbol Nomenclature : ACTR1B
SYNONYM : ARP1B; CTRN2; PC3
Designation : actin-related protein 1B|beta-centractin|centractin beta
Full Nomenclature : ARP1 actin-related protein 1 homolog B, centractin beta (yeast)
GENEID : 10120
GenBank Accession : DQ896748
HGNC : 168
MIM : 605144
Vega : OTTHUMG00000130549
Target GenBank: DQ896748

 

Reference Sequence Alignment

  

HsCD00437443      ATGGAGTCCTACGACATCATCGCCAACCAGCCTGTGGTCATCGACAACGGTTCGGGGGTG
NM_005735.3       ATGGAGTCCTACGACATCATCGCCAACCAGCCTGTGGTCATCGACAACGGTTCGGGGGTG
NM_005736.3       ATGGAGTCCTACGATGTGATCGCCAACCAGCCTGTCGTGATCGACAACGGATCCGGTGTG
                  ************** .* ***************** ** ***********:** ** ***

HsCD00437443      ATTAAAGCTGGCTTTGCAGGAGACCAGATTCCCAAATACTGTTTCCCAAACTATGTCGGG
NM_005735.3       ATTAAAGCTGGCTTTGCAGGAGACCAGATTCCCAAATACTGTTTCCCAAACTATGTCGGG
NM_005736.3       ATTAAAGCTGGTTTTGCTGGTGATCAGATCCCCAAATACTGCTTTCCAAACTATGTGGGC
                  *********** *****:**:** ***** *********** ** *********** ** 

HsCD00437443      CGGCCGAAGCACATGCGGGTGATGGCTGGAGCCCTGGAGGGGGACCTCTTCATCGGACCA
NM_005735.3       CGGCCGAAGCACATGCGGGTGATGGCTGGAGCCCTGGAGGGGGACCTCTTCATCGGACCA
NM_005736.3       CGACCCAAGCACGTTCGTGTCATGGCAGGAGCCCTTGAAGGCGACATCTTCATTGGCCCC
                  **.** ******.* ** ** *****:******** **.** ***.******* **.**.

HsCD00437443      AAAGCAGAGGAGCACCGGGGGCTGCTGACCATCCGCTACCCCATGGAGCACGGCGTGGTG
NM_005735.3       AAAGCAGAGGAGCACCGGGGGCTGCTGACCATCCGCTACCCCATGGAGCACGGCGTGGTG
NM_005736.3       AAAGCTGAGGAGCACCGAGGGCTGCTTTCAATCCGCTATCCCATGGAGCATGGCATCGTC
                  *****:***********.******** :*.******** *********** ***.* ** 

HsCD00437443      CGAGACTGGAACGACATGGAACGCATCTGGCAGTACGCCTACTCCAAGGATCAGCTGCAG
NM_005735.3       CGAGACTGGAACGACATGGAACGCATCTGGCAGTACGTCTACTCCAAGGATCAGCTGCAG
NM_005736.3       AAGGATTGGAACGACATGGAACGCATTTGGCAATATGTCTATTCTAAGGACCAGCTGCAG
                  ...** ******************** *****.** * *** ** ***** *********

HsCD00437443      ACCTTCTCGGAGGAGCATCCTGTGCTCCTCACGGAGGCCCCGCTCAACCCGAGTAAGAAC
NM_005735.3       ACCTTCTCGGAGGAGCATCCTGTGCTCCTCACGGAGGCCCCGCTCAACCCGAGTAAGAAC
NM_005736.3       ACTTTCTCAGAGGAGCATCCTGTGCTCCTGACTGAGGCGCCTTTAAACCCACGAAAAAAC
                  ** *****.******************** ** ***** **  *.*****..*:**.***

HsCD00437443      CGGGAGAAGGCGGCAGAGGTGTTCTTTGAGACCTTCAACGTGCCGGCCCTGTTCATCTCC
NM_005735.3       CGGGAGAAGGCGGCAGAGGTGTTCTTTGAGACCTTCAACGTGCCGGCCCTGTTCATCTCC
NM_005736.3       CGGGAACGAGCTGCCGAAGTTTTCTTCGAGACCTTCAATGTGCCCGCTCTTTTCATCTCC
                  *****....** **.**.** ***** *********** ***** ** ** *********

HsCD00437443      ATGCAGGCTGTGCTCAGTCTGTACGCAACAGGACGCACGACAGGAGTGGTTCTAGACTCA
NM_005735.3       ATGCAGGCTGTGCTCAGTCTGTACGCAACAGGACGCACGACAGGAGTGGTTCTAGACTCA
NM_005736.3       ATGCAAGCTGTACTCAGCCTTTACGCTACAGGCAGGACCACAGGGGTGGTGCTGGATTCT
                  *****.*****.***** ** *****:*****..* ** *****.***** **.** **:

HsCD00437443      GGGGACGGGGTCACTCATGCTGTGCCCATCTATGAGGGCTTTGCCATGCCTCACTCCATC
NM_005735.3       GGGGACGGGGTCACTCATGCTGTGCCCATCTATGAGGGCTTTGCCATGCCTCACTCCATC
NM_005736.3       GGGGATGGAGTCACCCATGCTGTGCCCATCTATGAGGGCTTTGCCATGCCCCACTCCATC
                  ***** **.***** *********************************** *********

HsCD00437443      ATGCGGGTGGACATTGCCGGCCGCGACGTCTCCCGCTACCTCCGACTCCTGCTGCGCAAG
NM_005735.3       ATGCGGGTGGACATTGCCGGCCGCGACGTCTCCCGCTACCTCCGACTCCTGCTGCGCAAG
NM_005736.3       ATGCGCATCGACATCGCGGGCCGGGACGTCTCTCGCTTCCTGCGCCTCTACCTGCGTAAG
                  ***** .* ***** ** ***** ******** ****:*** **.*** : ***** ***

HsCD00437443      GAAGGGGTTGACTTCCATACCTCGGCTGAGTTTGAGGTTGTCCGGACAATCAAAGAGCGA
NM_005735.3       GAAGGGGTTGACTTCCATACCTCGGCTGAGTTTGAGGTTGTCCGGACAATCAAAGAGCGA
NM_005736.3       GAGGGCTACGACTTCCACTCATCCTCTGAGTTTGAGATTGTCAAGGCCATAAAAGAAAGA
                  **.**  : ******** :*.**  ***********.*****..*.*.**.*****..**

HsCD00437443      GCGTGCTACCTGTCCATCAACCCACAGAAGGATGAGGCTCTGGAGACGGAGAAGGTGCAG
NM_005735.3       GCGTGCTACCTGTCCATCAACCCACAGAAGGATGAGGCTCTGGAGACGGAGAAGGTGCAG
NM_005736.3       GCCTGTTACCTATCCATAAACCCCCAAAAGGATGAGACGCTAGAGACAGAGAAAGCTCAG
                  ** ** *****.*****.*****.**.*********.* **.*****.*****.*  ***

HsCD00437443      TACACGTTGCCAGACGGCAGCACGCTTGATGTGGGGCCTGCACGATTCCGGGCCCCCGAG
NM_005735.3       TACACGTTGCCAGACGGCAGCACGCTTGATGTGGGGCCTGCACGATTCCGGGCCCCCGAG
NM_005736.3       TACTACCTGCCTGATGGCAGCACCATTGAGATTGGTCCTTCCCGATTCCGGGCCCCTGAG
                  ***:.  ****:** ******** .**** .* ** *** *.************** ***

HsCD00437443      CTGCTGTTCCAGCCGGACCTTGTGGGGGATGAGAGTGAGGGGCTCCATGAGGTGGTGGCC
NM_005735.3       CTGCTGTTCCAGCCGGACCTTGTCGGGGATGAGAGTGAGGGGCTCCATGAGGTGGTGGCC
NM_005736.3       TTGCTCTTCAGGCCAGATTTGATTGGAGAGGAGAGTGAAGGCATCCACGAGGTCCTGGTG
                   **** ***..***.**  * .* **.** ********.** .**** *****  ***  

HsCD00437443      TTCGCCATACACAAGTCCGACATGGACCTGCGCCGGACGCTGTTCGCCAACATCGTGCTC
NM_005735.3       TTCGCCATACACAAGTCCGACATGGACCTGCGCCGGACGCTGTTCGCCAACATCGTGCTC
NM_005736.3       TTCGCCATTCAGAAGTCAGACATGGACCTGCGGCGCACGCTTTTCTCTAACATTGTCCTC
                  ********:** *****.************** ** ***** *** * ***** ** ***

HsCD00437443      TCAGGTGGCTCAACGCTTTTCAAAGGCTTCGGAGACCGATTACTCAGTGAAGTGAAGAAG
NM_005735.3       TCAGGTGGCTCAACGCTTTTCAAAGGCTTCGGAGACCGATTACTCAGTGAAGTGAAGAAG
NM_005736.3       TCAGGAGGCTCTACCCTGTTCAAAGGTTTTGGTGACAGGCTCCTGAGTGAAGTGAAGAAA
                  *****:*****:** ** ******** ** **:***.*. *.** **************.

HsCD00437443      CTTGCCCCAAAGGATATCAAAATCAAGATCTCAGCCCCGCAGGAACGGCTGTACTCCACA
NM_005735.3       CTTGCCCCAAAGGATATCAAAATCAAGATCTCAGCCCCGCAGGAACGGCTGTACTCCACA
NM_005736.3       CTAGCTCCAAAAGATGTGAAGATCAGGATATCTGCACCTCAGGAGAGACTGTATTCCACG
                  **:** *****.***.* **.****.***.**:**.** *****..*.***** *****.

HsCD00437443      TGGATTGGCGGCTCCATCCTGGCCTCGCTGGACACTTTTAAGAAGATGTGGGTGTCCAAA
NM_005735.3       TGGATTGGCGGCTCCATCCTGGCCTCGCTGGACACTTTTAAGAAGATGTGGGTGTCCAAA
NM_005736.3       TGGATTGGGGGCTCCATCCTTGCCTCCCTGGACACCTTTAAGAAGATGTGGGTCTCCAAA
                  ******** *********** ***** ******** ***************** ******

HsCD00437443      AAGGAGTATGAAGAGGATGGCTCCCGTGCTATTCATCGCAAAACTTTC---
NM_005735.3       AAGGAGTATGAAGAGGATGGCTCCCGTGCTATTCATCGCAAAACTTTCTAG
NM_005736.3       AAGGAATATGAGGAAGACGGTGCCCGATCCATCCACAGAAAAACCTTCTAA
                  *****.*****.**.** **  ****: * ** ** .*.***** ***   

Pathways

Panther : Nicotinic acetylcholine receptor signaling pathway
Reactome : Adaptive Immune System
Reactome : Immune System
Reactome : MHC class II antigen presentation

Protein

SMART domain : ACTIN : Actin
HPRD : 05508

Clone

Original Clone ID : ccsbBroad304_07554

Organism


TAX_ID : 9606
Species Specific ID: 10120

Genome

Chromosome : 2
Map Location : 2q11.1-q11.2
Ensembl : ENSG00000115073

Reagents

Labome : ACTR1B-antibody