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SARNP (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  SARNP
Gene Name:  SAP domain containing ribonucleoprotein
Sequence             
Original Clone ID: ccsbBroad304_12810
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00437451

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1168nts         Open reading frame : 1 to 1168

ATGAAACGAATGGCAGAGAATGAGCTGAGCCGGTCAGTAAATGAGTTTCTGTCCAAGCTG
CAAGATGACCTCAAGGAGGCAATGAATACTATGATGTGTAGCCGATGCCAAGGAAAGCAT
AGGAGGTTTGAAATGGACCGGGAACCTAAGAGTGCCAGATACTGTGCTGAGTGTAATAGG
CTGCATCCTGCTGAGGAAGGAGACTTTTGGGCAGAGTCAAGCATGTTGGGCCTCAAGATC
ACCTACTTTGCACTGATGGATGGAAAGGTGTATGACATCACAGAGTGGGCTGGATGCCAG
CGTGTAGGTATCTCCCCAGATACCCACAGAGTCCCCTATCACATCTCATTTGGTTCTCGG
ATTCCAGGCACCAGAGGGCGGCAGAGAGCCACCCCAGATGCCCCTCCTGCTGATCTTCAG
GATTTCTTGAGTCGGATCTTTCAAGTACCCCCAGGGCAGATGCCCAATGGGAACTTCTTT
GCAGCTCCTCAGCCTGCCCCTGGAGCCGCTGCAGCCTCTAAGCCCAACAGCACAGTACCC
AAGGGAGAAGCCAAACCTAAGCGGCGGAAGAAACTTGCCGAACTAAAGCAAGAATGTCTT
GCTCGTGGTTTGGAGACCAAGGGAATAAAGCAAGATCTTATCCACAGACTCCAGGCATAT
CTTGAAGAACATGCTGAAGAGGAGGCAAATGAAGAAGATGTACTGGGAGATGAAACAGAG
GAAGAAGAAACAAAGCCCATTGAGCTCCCTGTCAAAGAGGAAGAACCCCCTGAAAAAACT
GTTGATGTGGCAGCAGAGAAGAAAGTGGTGAAAATTACATCTGAAATACCACAGACTGAG
AGAATGCAGAAGAGGGCTGAACGATTCAATGTACCTGTGAGCTTGGAGAGTAAGAAAGTT
GCTCGGGCAGCTAGGTTTGGGATTTCTTCAGTTCCAACAAAAGGTCTGTCATCTGATAAC
AAACCTATGGTTAACTTGGATAAGCTGAAGGAAAGAGCTCAAAGATTTGGTTTGAATGTC
TCTTCAATCTCCAGAAAGTCTGAAGATGATGAGAAACTGAAAAAGAGGAAGGAGCGATTT
GGGATTGTCACAAGTTCAGCTGGAACTGGAACCACAGAGGATACAGAGGCAAAGAAGAGG
AAAAGAGCAGAGCGCTTTGGGATTGCC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : SARNP
Symbol Nomenclature : SARNP
SYNONYM : CIP29; HCC1; HSPC316; THO1
Designation : SAP domain-containing ribonucleoprotein|cytokine induced protein 29 kDa|cytokine-induced protein of 29 kDa|hepatocellular carcinoma 1|nuclear protein Hcc-1|proliferation associated cytokine-inducible protein CIP29|proliferation-associated cytokine-inducible protein CIP29
Full Nomenclature : SAP domain containing ribonucleoprotein
GENEID : 84324
GenBank Accession : EU446946
HGNC : 24432
MIM : 610049
Vega : OTTHUMG00000170441
Target GenBank: EU446946

 

Clone

Original Clone ID : ccsbBroad304_12810

Organism


TAX_ID : 9606
Species Specific ID: 84324

Genome

Chromosome : 12
Map Location : 12q13.2
Ensembl : ENSG00000205323

Reagents

Labome : SARNP-antibody