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SRPR (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  SRPR
Gene Name:  signal recognition particle receptor (docking protein)
Sequence             
Original Clone ID: ccsbBroad304_11158
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00437494

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1729nts         Open reading frame : 1 to 1729
Reference Sequence Alignment

ATGCTCGACTTCTTCACCATTTTCTCCAAGGGCGGGCTTGTGCTCTGGTGCTTCCAGGGC
GTTAGCGACTCATGCACCGGACCCGTTAACGCGTTGATTCGTTCCGTGCTGCTGCAGGAA
CGGGGAGGTAACAACTCCTTCACCCATGAGGCACTCACACTCAAGTATAAACTGGACAAC
CAGTTTGAGCTGGTGTTTGTGGTTGGTTTTCAGAAGATCCTGACACTGACATATGTAGAC
AAATTGATAGATGACGTGCATCGGCTGTTTCGGGACAAGTACCGCACAGAGATCCAACAG
CAAAGTGCTTTAAGTTTATTAAATGGCACTTTTGATTTCCAAAATGACTTCCTGCGGCTC
CTTCGTGAAGCAGAGGAGAGCAGTAAGATCCGTGCTCCCACTACCATGAAGAAATTTGAA
GATTCTGAAAAGGCCAAGAAACCTGTGAGGTCCATGATTGAGACACGGGGGGAAAAGCCC
AAGGAAAAAGCAAAGAATAGCAAAAAAAAGGGGGCCAAGAAGGAAGGTTCTGATGGTCCT
TTGGCTACCAGCAAACCAGTCCCTGCAGAAAAGTCAGGTCTTCCAGTGGGTCCTGAGAAC
GGAGTAGAACTTTCCAAAGAGGAGCTGATCCGCAGGAAGCGCGAGGAGTTCATTCAGAAG
CATGGGAGGGGTATGGAGAAGTCCAACAAGTCCACGAAGTCAGATGCTCCAAAGGAGAAG
GGCAAAAAAGCACCCCGGGTGTGGGAACTGGGTGGCTGTGCTAACAAAGAAGTGTTGGAT
TACAGTACTCCCACCACCAATGGAACCCCTGAGGCTGCCTTGTCTGAGGACATCAACCTG
ATTCGAGGGACTGGGTCTGGGGGGCAGCTTCAGGATCTGGACTGCAGCAGCTCTGATGAC
GAAGGGGCTGCTCAAAACTCTACCAAACCTAGTGCGACCAAGGGAACACTGGGTGGCATG
TTTGGTATGCTGAAGGGCCTTGTGGGTTCAAAGAGCTTGAGTCGTGAAGACATGGAATCT
GTGCTGGACAAGATGCGTGATCATCTCATTGCTAAGAACGTGGCTGCAGACATTGCCGTC
CAGCTCTGTGAATCTGTTGCCAACAAGTTGGAAGGGAAGGTGATGGGGACGTTCAGCACG
GTGACTTCCACAGTAAAGCAAGCCCTACAGGAGTCCCTGGTGCAGATTCTGCAGCCACAG
CGTCGTGTAGACATGCTCCGGGACATCATGGATGCCCAGCGTCGCCAGCGCCCTTATGTC
GTCACCTTCTGCGGCGTTAATGGAGTGGGGAAATCTACTAATCTTGCCAAGATTTCCTTC
TGGTTGTTAGAGAATGGCTTCAGTGTCCTCATTGCTGCCTGTGATACATTTCGTGCTGGG
GCCGTGGAGCAGCTGCGTACACACACCCGGCGTTTGAGTGCCCTACACCCTCCAGAGAAG
CATGGTGGCCGCACCATGGTGCAGTTGTTTGAAAAGGGCTATGGCAAGGATGCTGCTGGC
ATTGCCATGGAAGCCATTGCTTTTGCACGTAACCAAGGCTTTGACGTGGTGCTGGTGGAC
ACGGCAGGCCGCATGCAAGACAATGCCCCTCTGATGACTGCCCTGGCCAAACTCATTACT
GTCAATACACCTGATTTGGTGCTGTTTGTAGGAGAAGCCTTAGTAGGCAATGAAGCCGTG
GACCAGCTGGTCAAGTTCAACAGAGCCTTGGCTGACCATTCTATGGCT


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : SRPR
Symbol Nomenclature : SRPR
SYNONYM : DP; Sralpha
Designation : DP-alpha|SR-alpha|SRP-alpha|docking protein alpha|signal recognition particle receptor subunit alpha
Full Nomenclature : signal recognition particle receptor (docking protein)
GENEID : 6734
HGNC : 11307
MIM : 182180
Vega : OTTHUMG00000165826
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00437494        ATGCTCGACTTCTTCACCATTTTCTCCAAGGGCGGGCTTGTGCTCTGGTGCTTCCAGGGC
NM_003139.3         ATGCTCGACTTCTTCACCATTTTCTCCAAGGGCGGGCTTGTGCTCTGGTGCTTCCAGGGC
NM_001177842.1      ATGCTCGACTTCTTCACCATTTTCTCCAAGGGCGGGCTTGTGCTCTGGTGCTTCCAGGGC
                    ************************************************************

HsCD00437494        GTTAGCGACTCATGCACCGGACCCGTTAACGCGTTGATTCGTTCCGTGCTGCTGCAGGAA
NM_003139.3         GTTAGCGACTCATGCACCGGACCCGTTAACGCGTTGATTCGTTCCGTGCTGCTGCAGGAA
NM_001177842.1      GTTAGCGACTCATGCACCGGACCCGTTAACGCGTTGATTCGTTCCGTGCTGCTGCAG---
                    *********************************************************   

HsCD00437494        CGGGGAGGTAACAACTCCTTCACCCATGAGGCACTCACACTCAAGTATAAACTGGACAAC
NM_003139.3         CGGGGAGGTAACAACTCCTTCACCCATGAGGCACTCACACTCAAGTATAAACTGGACAAC
NM_001177842.1      ------------------------------------------------------------
                                                                                

HsCD00437494        CAGTTTGAGCTGGTGTTTGTGGTTGGTTTTCAGAAGATCCTGACACTGACATATGTAGAC
NM_003139.3         CAGTTTGAGCTGGTGTTTGTGGTTGGTTTTCAGAAGATCCTGACACTGACATATGTAGAC
NM_001177842.1      ---------------------GTTGGTTTTCAGAAGATCCTGACACTGACATATGTAGAC
                                         ***************************************

HsCD00437494        AAATTGATAGATGACGTGCATCGGCTGTTTCGGGACAAGTACCGCACAGAGATCCAACAG
NM_003139.3         AAATTGATAGATGACGTGCATCGGCTGTTTCGGGACAAGTACCGCACAGAGATCCAACAG
NM_001177842.1      AAATTGATAGATGACGTGCATCGGCTGTTTCGGGACAAGTACCGCACAGAGATCCAACAG
                    ************************************************************

HsCD00437494        CAAAGTGCTTTAAGTTTATTAAATGGCACTTTTGATTTCCAAAATGACTTCCTGCGGCTC
NM_003139.3         CAAAGTGCTTTAAGTTTATTAAATGGCACTTTTGATTTCCAAAATGACTTCCTGCGGCTC
NM_001177842.1      CAAAGTGCTTTAAGTTTATTAAATGGCACTTTTGATTTCCAAAATGACTTCCTGCGGCTC
                    ************************************************************

HsCD00437494        CTTCGTGAAGCAGAGGAGAGCAGTAAGATCCGTGCTCCCACTACCATGAAGAAATTTGAA
NM_003139.3         CTTCGTGAAGCAGAGGAGAGCAGTAAGATCCGTGCTCCCACTACCATGAAGAAATTTGAA
NM_001177842.1      CTTCGTGAAGCAGAGGAGAGCAGTAAGATCCGTGCTCCCACTACCATGAAGAAATTTGAA
                    ************************************************************

HsCD00437494        GATTCTGAAAAGGCCAAGAAACCTGTGAGGTCCATGATTGAGACACGGGGGGAAAAGCCC
NM_003139.3         GATTCTGAAAAGGCCAAGAAACCTGTGAGGTCCATGATTGAGACACGGGGGGAAAAGCCC
NM_001177842.1      GATTCTGAAAAGGCCAAGAAACCTGTGAGGTCCATGATTGAGACACGGGGGGAAAAGCCC
                    ************************************************************

HsCD00437494        AAGGAAAAAGCAAAGAATAGCAAAAAAAAGGGGGCCAAGAAGGAAGGTTCTGATGGTCCT
NM_003139.3         AAGGAAAAAGCAAAGAATAGCAAAAAAAAGGGGGCCAAGAAGGAAGGTTCTGATGGTCCT
NM_001177842.1      AAGGAAAAAGCAAAGAATAGCAAAAAAAAGGGGGCCAAGAAGGAAGGTTCTGATGGTCCT
                    ************************************************************

HsCD00437494        TTGGCTACCAGCAAACCAGTCCCTGCAGAAAAGTCAGGTCTTCCAGTGGGTCCTGAGAAC
NM_003139.3         TTGGCTACCAGCAAACCAGTCCCTGCAGAAAAGTCAGGTCTTCCAGTGGGTCCTGAGAAC
NM_001177842.1      TTGGCTACCAGCAAACCAGTCCCTGCAGAAAAGTCAGGTCTTCCAGTGGGTCCTGAGAAC
                    ************************************************************

HsCD00437494        GGAGTAGAACTTTCCAAAGAGGAGCTGATCCGCAGGAAGCGCGAGGAGTTCATTCAGAAG
NM_003139.3         GGAGTAGAACTTTCCAAAGAGGAGCTGATCCGCAGGAAGCGCGAGGAGTTCATTCAGAAG
NM_001177842.1      GGAGTAGAACTTTCCAAAGAGGAGCTGATCCGCAGGAAGCGCGAGGAGTTCATTCAGAAG
                    ************************************************************

HsCD00437494        CATGGGAGGGGTATGGAGAAGTCCAACAAGTCCACGAAGTCAGATGCTCCAAAGGAGAAG
NM_003139.3         CATGGGAGGGGTATGGAGAAGTCCAACAAGTCCACGAAGTCAGATGCTCCAAAGGAGAAG
NM_001177842.1      CATGGGAGGGGTATGGAGAAGTCCAACAAGTCCACGAAGTCAGATGCTCCAAAGGAGAAG
                    ************************************************************

HsCD00437494        GGCAAAAAAGCACCCCGGGTGTGGGAACTGGGTGGCTGTGCTAACAAAGAAGTGTTGGAT
NM_003139.3         GGCAAAAAAGCACCCCGGGTGTGGGAACTGGGTGGCTGTGCTAACAAAGAAGTGTTGGAT
NM_001177842.1      GGCAAAAAAGCACCCCGGGTGTGGGAACTGGGTGGCTGTGCTAACAAAGAAGTGTTGGAT
                    ************************************************************

HsCD00437494        TACAGTACTCCCACCACCAATGGAACCCCTGAGGCTGCCTTGTCTGAGGACATCAACCTG
NM_003139.3         TACAGTACTCCCACCACCAATGGAACCCCTGAGGCTGCCTTGTCTGAGGACATCAACCTG
NM_001177842.1      TACAGTACTCCCACCACCAATGGAACCCCTGAGGCTGCCTTGTCTGAGGACATCAACCTG
                    ************************************************************

HsCD00437494        ATTCGAGGGACTGGGTCTGGGGGGCAGCTTCAGGATCTGGACTGCAGCAGCTCTGATGAC
NM_003139.3         ATTCGAGGGACTGGGTCTGGGGGGCAGCTTCAGGATCTGGACTGCAGCAGCTCTGATGAC
NM_001177842.1      ATTCGAGGGACTGGGTCTGGGGGGCAGCTTCAGGATCTGGACTGCAGCAGCTCTGATGAC
                    ************************************************************

HsCD00437494        GAAGGGGCTGCTCAAAACTCTACCAAACCTAGTGCGACCAAGGGAACACTGGGTGGCATG
NM_003139.3         GAAGGGGCTGCTCAAAACTCTACCAAACCTAGTGCGACCAAGGGAACACTGGGTGGCATG
NM_001177842.1      GAAGGGGCTGCTCAAAACTCTACCAAACCTAGTGCGACCAAGGGAACACTGGGTGGCATG
                    ************************************************************

HsCD00437494        TTTGGTATGCTGAAGGGCCTTGTGGGTTCAAAGAGCTTGAGTCGTGAAGACATGGAATCT
NM_003139.3         TTTGGTATGCTGAAGGGCCTTGTGGGTTCAAAGAGCTTGAGTCGTGAAGACATGGAATCT
NM_001177842.1      TTTGGTATGCTGAAGGGCCTTGTGGGTTCAAAGAGCTTGAGTCGTGAAGACATGGAATCT
                    ************************************************************

HsCD00437494        GTGCTGGACAAGATGCGTGATCATCTCATTGCTAAGAACGTGGCTGCAGACATTGCCGTC
NM_003139.3         GTGCTGGACAAGATGCGTGATCATCTCATTGCTAAGAACGTGGCTGCAGACATTGCCGTC
NM_001177842.1      GTGCTGGACAAGATGCGTGATCATCTCATTGCTAAGAACGTGGCTGCAGACATTGCCGTC
                    ************************************************************

HsCD00437494        CAGCTCTGTGAATCTGTTGCCAACAAGTTGGAAGGGAAGGTGATGGGGACGTTCAGCACG
NM_003139.3         CAGCTCTGTGAATCTGTTGCCAACAAGTTGGAAGGGAAGGTGATGGGGACGTTCAGCACG
NM_001177842.1      CAGCTCTGTGAATCTGTTGCCAACAAGTTGGAAGGGAAGGTGATGGGGACGTTCAGCACG
                    ************************************************************

HsCD00437494        GTGACTTCCACAGTAAAGCAAGCCCTACAGGAGTCCCTGGTGCAGATTCTGCAGCCACAG
NM_003139.3         GTGACTTCCACAGTAAAGCAAGCCCTACAGGAGTCCCTGGTGCAGATTCTGCAGCCACAG
NM_001177842.1      GTGACTTCCACAGTAAAGCAAGCCCTACAGGAGTCCCTGGTGCAGATTCTGCAGCCACAG
                    ************************************************************

HsCD00437494        CGTCGTGTAGACATGCTCCGGGACATCATGGATGCCCAGCGTCGCCAGCGCCCTTATGTC
NM_003139.3         CGTCGTGTAGACATGCTCCGGGACATCATGGATGCCCAGCGTCGCCAGCGCCCTTATGTC
NM_001177842.1      CGTCGTGTAGACATGCTCCGGGACATCATGGATGCCCAGCGTCGCCAGCGCCCTTATGTC
                    ************************************************************

HsCD00437494        GTCACCTTCTGCGGCGTTAATGGAGTGGGGAAATCTACTAATCTTGCCAAGATTTCCTTC
NM_003139.3         GTCACCTTCTGCGGCGTTAATGGAGTGGGGAAATCTACTAATCTTGCCAAGATTTCCTTC
NM_001177842.1      GTCACCTTCTGCGGCGTTAATGGAGTGGGGAAATCTACTAATCTTGCCAAGATTTCCTTC
                    ************************************************************

HsCD00437494        TGGTTGTTAGAGAATGGCTTCAGTGTCCTCATTGCTGCCTGTGATACATTTCGTGCTGGG
NM_003139.3         TGGTTGTTAGAGAATGGCTTCAGTGTCCTCATTGCTGCCTGTGATACATTTCGTGCTGGG
NM_001177842.1      TGGTTGTTAGAGAATGGCTTCAGTGTCCTCATTGCTGCCTGTGATACATTTCGTGCTGGG
                    ************************************************************

HsCD00437494        GCCGTGGAGCAGCTGCGTACACACACCCGGCGTTTGAGTGCCCTACACCCTCCAGAGAAG
NM_003139.3         GCCGTGGAGCAGCTGCGTACACACACCCGGCGTTTGAGTGCCCTACACCCTCCAGAGAAG
NM_001177842.1      GCCGTGGAGCAGCTGCGTACACACACCCGGCGTTTGAGTGCCCTACACCCTCCAGAGAAG
                    ************************************************************

HsCD00437494        CATGGTGGCCGCACCATGGTGCAGTTGTTTGAAAAGGGCTATGGCAAGGATGCTGCTGGC
NM_003139.3         CATGGTGGCCGCACCATGGTGCAGTTGTTTGAAAAGGGCTATGGCAAGGATGCTGCTGGC
NM_001177842.1      CATGGTGGCCGCACCATGGTGCAGTTGTTTGAAAAGGGCTATGGCAAGGATGCTGCTGGC
                    ************************************************************

HsCD00437494        ATTGCCATGGAAGCCATTGCTTTTGCACGTAACCAAGGCTTTGACGTGGTGCTGGTGGAC
NM_003139.3         ATTGCCATGGAAGCCATTGCTTTTGCACGTAACCAAGGCTTTGACGTGGTGCTGGTGGAC
NM_001177842.1      ATTGCCATGGAAGCCATTGCTTTTGCACGTAACCAAGGCTTTGACGTGGTGCTGGTGGAC
                    ************************************************************

HsCD00437494        ACGGCAGGCCGCATGCAAGACAATGCCCCTCTGATGACTGCCCTGGCCAAACTCATTACT
NM_003139.3         ACGGCAGGCCGCATGCAAGACAATGCCCCTCTGATGACTGCCCTGGCCAAACTCATTACT
NM_001177842.1      ACGGCAGGCCGCATGCAAGACAATGCCCCTCTGATGACTGCCCTGGCCAAACTCATTACT
                    ************************************************************

HsCD00437494        GTCAATACACCTGATTTGGTGCTGTTTGTAGGAGAAGCCTTAGTAGGCAATGAAGCCGTG
NM_003139.3         GTCAATACACCTGATTTGGTGCTGTTTGTAGGAGAAGCCTTAGTAGGCAATGAAGCCGTG
NM_001177842.1      GTCAATACACCTGATTTGGTGCTGTTTGTAGGAGAAGCCTTAGTAGGCAATGAAGCCGTG
                    ************************************************************

HsCD00437494        GACCAGCTGGTCAAGTTCAACAGAGCCTTGGCTGACCATTCTATGGCT------------
NM_003139.3         GACCAGCTGGTCAAGTTCAACAGAGCCTTGGCTGACCATTCTATGGCTCAGACACCTCGG
NM_001177842.1      GACCAGCTGGTCAAGTTCAACAGAGCCTTGGCTGACCATTCTATGGCTCAGACACCTCGG
                    ************************************************            

HsCD00437494        ------------------------------------------------------------
NM_003139.3         CTCATTGATGGCATTGTTCTTACCAAATTTGATACCATTGATGACAAGGTGGGAGCTGCT
NM_001177842.1      CTCATTGATGGCATTGTTCTTACCAAATTTGATACCATTGATGACAAGGTGGGAGCTGCT
                                                                                

HsCD00437494        ------------------------------------------------------------
NM_003139.3         ATTTCTATGACGTACATCACAAGCAAACCCATCGTCTTTGTGGGCACCGGCCAGACCTAC
NM_001177842.1      ATTTCTATGACGTACATCACAAGCAAACCCATCGTCTTTGTGGGCACCGGCCAGACCTAC
                                                                                

HsCD00437494        ---------------------------------------------------------
NM_003139.3         TGTGACCTACGCAGCCTCAATGCCAAGGCTGTGGTGGCTGCCCTCATGAAGGCTTAA
NM_001177842.1      TGTGACCTACGCAGCCTCAATGCCAAGGCTGTGGTGGCTGCCCTCATGAAGGCTTAA
                                                                             

Pathways

Reactome : Gene Expression
Reactome : IRE1alpha activates chaperones
Reactome : Metabolism of proteins
Reactome : SRP-dependent cotranslational protein targeting to membrane
Reactome : Translation
Reactome : Unfolded Protein Response (UPR)
Reactome : XBP1(S) activates chaperone genes

Clone

Original Clone ID : ccsbBroad304_11158

Organism


TAX_ID : 9606
Species Specific ID: 6734

Genome

Chromosome : 11
Map Location : 11q24.2
Ensembl : ENSG00000182934

Reagents

Labome : SRPR-antibody