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LGALS8 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  LGALS8
Gene Name:  lectin, galactoside-binding, soluble, 8
Sequence             
Original Clone ID: ccsbBroad304_10438
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00438147

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1075nts         Open reading frame : 1 to 1075
Reference Sequence Alignment

ATGTTGTCCTTAAACAACCTACAGAATATCATCTATAACCCGGTAATCCCGTATGTTGGC
ACCATTCCCGATCAGCTGGATCCTGGAACTTTGATTGTGATATGTGGGCATGTTCCTAGT
GACGCAGACAGATTCCAGGTGGATCTGCAGAATGGCAGCAGTGTGAAACCTCGAGCCGAT
GTGGCCTTTCATTTCAATCCTCGTTTCAAAAGGGCCGGCTGCATTGTTTGCAATACTTTG
ATAAATGAAAAATGGGGACGGGAAGAGATCACCTATGACACGCCTTTCAAAAGAGAAAAG
TCTTTTGAGATCGTGATTATGGTGCTAAAGGACAAATTCCAGGTGGCTGTAAATGGAAAA
CATACTCTGCTCTATGGCCACAGGATCGGCCCAGAGAAAATAGACACTCTGGGCATTTAT
GGCAAAGTGAATATTCACTCAATTGGTTTTAGCTTCAGCTCGGACTTACAAAGTACCCAA
GCATCTAGTCTGGAACTGACAGAGATAAGTAGAGAAAATGTTCCAAAGTCTGGCACGCCC
CAGCTTCCTAGTAATAGAGGAGGAGACATTTCTAAAATCGCACCCAGAACTGTCTACACC
AAGAGCAAAGATTCGACTGTCAATCACACTTTGACTTGCACCAAAATACCACCTATGAAC
TATGTGTCAAAGAGCCTGCCATTCGCTGCAAGGTTGAACACCCCCATGGGCCCTGGACGA
ACTGTCGTCGTTAAAGGAGAAGTGAATGCAAATGCCAAAAGCTTTAATGTTGACCTACTA
GCAGGAAAATCAAAGGATATTGCTCTACACTTGAACCCACGCCTGAATATTAAAGCATTT
GTAAGAAATTCTTTTCTTCAGGAGTCCTGGGGAGAAGAAGAGAGAAATATTACCTCTTTC
CCATTTAGTCCTGGGATGTACTTTGAGATGATAATTTACTGTGATGTTAGAGAATTCAAG
GTTGCAGTAAATGGCGTACACAGCCTGGAGTACAAACACAGATTTAAAGAGCTCAGCAGT
ATTGACACGCTGGAAATTAATGGAGACATCCACTTACTGGAAGTATGGAGCTGG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
1 error; 1 non-synonomous errors
                                

5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : LGALS8
Symbol Nomenclature : LGALS8
SYNONYM : Gal-8; PCTA-1; PCTA1; Po66-CBP
Designation : Po66 carbohydrate binding protein|galectin 8|galectin-8|galectin-8g|po66 carbohydrate-binding protein|prostate carcinoma tumor antigen 1
Full Nomenclature : lectin, galactoside-binding, soluble, 8
GENEID : 3964
Locus Tag : RP11-385F5.1
HGNC : 6569
MIM : 606099
Vega : OTTHUMG00000039953
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00438147      ---ATGTTGTCCTTAAACAACCTACAGAATATCATCTATAACCCGGTAATCCCGTATGTT
NM_006499.4       ATGATGTTGTCCTTAAACAACCTACAGAATATCATCTATAACCCGGTAATCCCGTTTGTT
NM_201545.2       ATGATGTTGTCCTTAAACAACCTACAGAATATCATCTATAACCCGGTAATCCCGTTTGTT
                     ****************************************************:****

HsCD00438147      GGCACCATTCCCGATCAGCTGGATCCTGGAACTTTGATTGTGATATGTGGGCATGTTCCT
NM_006499.4       GGCACCATTCCTGATCAGCTGGATCCTGGAACTTTGATTGTGATACGTGGGCATGTTCCT
NM_201545.2       GGCACCATTCCTGATCAGCTGGATCCTGGAACTTTGATTGTGATACGTGGGCATGTTCCT
                  *********** ********************************* **************

HsCD00438147      AGTGACGCAGACAGATTCCAGGTGGATCTGCAGAATGGCAGCAGTGTGAAACCTCGAGCC
NM_006499.4       AGTGACGCAGACAGATTCCAGGTGGATCTGCAGAATGGCAGCAGCATGAAACCTCGAGCC
NM_201545.2       AGTGACGCAGACAGATTCCAGGTGGATCTGCAGAATGGCAGCAGCATGAAACCTCGAGCC
                  ******************************************** .**************

HsCD00438147      GATGTGGCCTTTCATTTCAATCCTCGTTTCAAAAGGGCCGGCTGCATTGTTTGCAATACT
NM_006499.4       GATGTGGCCTTTCATTTCAATCCTCGTTTCAAAAGGGCCGGCTGCATTGTTTGCAATACT
NM_201545.2       GATGTGGCCTTTCATTTCAATCCTCGTTTCAAAAGGGCCGGCTGCATTGTTTGCAATACT
                  ************************************************************

HsCD00438147      TTGATAAATGAAAAATGGGGACGGGAAGAGATCACCTATGACACGCCTTTCAAAAGAGAA
NM_006499.4       TTGATAAATGAAAAATGGGGACGGGAAGAGATCACCTATGACACGCCTTTCAAAAGAGAA
NM_201545.2       TTGATAAATGAAAAATGGGGACGGGAAGAGATCACCTATGACACGCCTTTCAAAAGAGAA
                  ************************************************************

HsCD00438147      AAGTCTTTTGAGATCGTGATTATGGTGCTAAAGGACAAATTCCAGGTGGCTGTAAATGGA
NM_006499.4       AAGTCTTTTGAGATCGTGATTATGGTGCTGAAGGACAAATTCCAGGTGGCTGTAAATGGA
NM_201545.2       AAGTCTTTTGAGATCGTGATTATGGTGCTGAAGGACAAATTCCAGGTGGCTGTAAATGGA
                  *****************************.******************************

HsCD00438147      AAACATACTCTGCTCTATGGCCACAGGATCGGCCCAGAGAAAATAGACACTCTGGGCATT
NM_006499.4       AAACATACTCTGCTCTATGGCCACAGGATCGGCCCAGAGAAAATAGACACTCTGGGCATT
NM_201545.2       AAACATACTCTGCTCTATGGCCACAGGATCGGCCCAGAGAAAATAGACACTCTGGGCATT
                  ************************************************************

HsCD00438147      TATGGCAAAGTGAATATTCACTCAATTGGTTTTAGCTTCAGCTCGGACTTACAAAGTACC
NM_006499.4       TATGGCAAAGTGAATATTCACTCAATTGGTTTTAGCTTCAGCTCGGACTTACAAAGTACC
NM_201545.2       TATGGCAAAGTGAATATTCACTCAATTGGTTTTAGCTTCAGCTCGGACTTACAAAGTACC
                  ************************************************************

HsCD00438147      CAAGCATCTAGTCTGGAACTGACAGAGATAAGTAGAGAAAATGTTCCAAAGTCTGGCACG
NM_006499.4       CAAGCATCTAGTCTGGAACTGACAGAGATAAGTAGAGAAAATGTTCCAAAGTCTGGCACG
NM_201545.2       CAAGCATCTAGTCTGGAACTGACAGAGATAAGTAGAGAAAATGTTCCAAAGTCTGGCACG
                  ************************************************************

HsCD00438147      CCCCAGCTTCCTAGTAATAGAGGAGGAGACATTTCTAAAATCGCACCCAGAACTGTCTAC
NM_006499.4       CCCCAGCTTCCTAGTAATAGAGGAGGAGACATTTCTAAAATCGCACCCAGAACTGTCTAC
NM_201545.2       CCCCAGCTTCCTAGTAATAGAGGAGGAGACATTTCTAAAATCGCACCCAGAACTGTCTAC
                  ************************************************************

HsCD00438147      ACCAAGAGCAAAGATTCGACTGTCAATCACACTTTGACTTGCACCAAAATACCACCTATG
NM_006499.4       ACCAAGAGCAAAGATTCGACTGTCAATCACACTTTGACTTGCACCAAAATACCACCTATG
NM_201545.2       ACCAAGAGCAAAGATTCGACTGTCAATCACACTTTGACTTGCACCAAAATACCACCTATG
                  ************************************************************

HsCD00438147      AACTATGTGTCAAAGAGCCTGCCATTCGCTGCAAGGTTGAACACCCCCATGGGCCCTGGA
NM_006499.4       AACTATGTGTCAAAGAGGCTGCCATTCGCTGCAAGGTTGAACACCCCCATGGGCCCTGGA
NM_201545.2       AACTATGTGTCAAAGAGGCTGCCATTCGCTGCAAGGTTGAACACCCCCATGGGCCCTGGA
                  ***************** ******************************************

HsCD00438147      CGAACTGTCGTCGTTAAAGGAGAAGTGAATGCAAATGCCAAAAGCTTTAATGTTGACCTA
NM_006499.4       CGAACTGTCGTCGTTAAAGGAGAAGTGAATGCAAATGCCAAAAGCTTTAATGTTGACCTA
NM_201545.2       CGAACTGTCGTCGTTAAAGGAGAAGTGAATGCAAATGCCAAAAGCTTTAATGTTGACCTA
                  ************************************************************

HsCD00438147      CTAGCAGGAAAATCAAAGGATATTGCTCTACACTTGAACCCACGCCTGAATATTAAAGCA
NM_006499.4       CTAGCAGGAAAATCAAAGGATATTGCTCTACACTTGAACCCACGCCTGAATATTAAAGCA
NM_201545.2       CTAGCAGGAAAATCAAAGGATATTGCTCTACACTTGAACCCACGCCTGAATATTAAAGCA
                  ************************************************************

HsCD00438147      TTTGTAAGAAATTCTTTTCTTCAGGAGTCCTGGGGAGAAGAAGAGAGAAATATTACCTCT
NM_006499.4       TTTGTAAGAAATTCTTTTCTTCAGGAGTCCTGGGGAGAAGAAGAGAGAAATATTACCTCT
NM_201545.2       TTTGTAAGAAATTCTTTTCTTCAGGAGTCCTGGGGAGAAGAAGAGAGAAATATTACCTCT
                  ************************************************************

HsCD00438147      TTCCCATTTAGTCCTGGGATGTACTTTGAGATGATAATTTACTGTGATGTTAGAGAATTC
NM_006499.4       TTCCCATTTAGTCCTGGGATGTACTTTGAGATGATAATTTATTGTGATGTTAGAGAATTC
NM_201545.2       TTCCCATTTAGTCCTGGGATGTACTTTGAGATGATAATTTATTGTGATGTTAGAGAATTC
                  ***************************************** ******************

HsCD00438147      AAGGTTGCAGTAAATGGCGTACACAGCCTGGAGTACAAACACAGATTTAAAGAGCTCAGC
NM_006499.4       AAGGTTGCAGTAAATGGCGTACACAGCCTGGAGTACAAACACAGATTTAAAGAGCTCAGC
NM_201545.2       AAGGTTGCAGTAAATGGCGTACACAGCCTGGAGTACAAACACAGATTTAAAGAGCTCAGC
                  ************************************************************

HsCD00438147      AGTATTGACACGCTGGAAATTAATGGAGACATCCACTTACTGGAAGTATGGAGCTGG---
NM_006499.4       AGTATTGACACGCTGGAAATTAATGGAGACATCCACTTACTGGAAGTAAGGAGCTGGTAG
NM_201545.2       AGTATTGACACGCTGGAAATTAATGGAGACATCCACTTACTGGAAGTAAGGAGCTGGTAG
                  ************************************************:********   

Protein

SMART domain : Gal-bind_lectin : Galactoside-binding lectin
SMART domain : GLECT : Galectin
HPRD : 09356

Clone

Original Clone ID : ccsbBroad304_10438

Organism


TAX_ID : 9606
Species Specific ID: 3964

Genome

Chromosome : 1
Map Location : 1q43
Ensembl : ENSG00000116977

Reagents

Labome : LGALS8-antibody