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ACTRT1 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ACTRT1
Gene Name:  actin-related protein T1
Sequence             
Original Clone ID: ccsbBroad304_04932
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00438152

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1129nts        
Open reading frame : 1 to 1129
Reference Sequence Alignment

ATGTTTAATCCACATGCATTAGATGTTCCTGCTGTAATTTTTGACAATGGTTCAGGACTC
TGCAAAGCAGGCCTGTCTGGAGAGATTGGACCCCGCCATGTCATCAGCTCCGTCTTGGGA
CATTGTAAATTCAATGTGCCTTTAGCAAGACTTAATCAGAAGTACTTCGTGGGGCAAGAA
GCCCTGTACAAGTATGAGGCCCTACATTTGCACTACCCCATTGAGCGTGGACTGGTAACA
GGATGGGATGACATGGAGAAACTCTGGAAACATCTCTTTGAGCGGGAGCTTGGAGTAAAA
CCCAGCCAACAGCCTGTACTTATGACCGAGCCCTCTTTGAATCCTAGGGAAATTCGAGAA
AAGCTAGCAGAAATGATGTTTGAGACCTTCAGTGTGCCTGGTTTCTACCTGTCTAATCAT
GCGGTGGCAGCGCTCTATGCCTCTGCCTGTGTCACAGGCCTGGTGGTGGACAGTGGAGAT
GGGGTCACTTGCACTGTCCCCATCTTTGAGGGTTACTCCCTGCCTCACGCAGTCACCAAA
CTCTGTATGGCAGGGAGGGACATCACAGAGCACCTCACCCGGCTCCTCTTTGCTAGCGGG
TTTAACTTCCCTTGCATACTCAACAAGGCCGTGGTAAATAACATCAAAGAGAAGTTGTGC
TACATCGCCTTGGAGCCAGAGAAAGAGCTACGCAAGAGCCGGGGAGAGGTCCTGGGAGCA
TACAGACTGCCAGATGGACATGTCATCCACTTTGGGGATGAGCTGTACCAAGTGCCCGAG
GTTCTTTTTGCACCTGACCAGCTGGGCATCCACAGCCCAGGACTCTCAAAAATGGTCTCC
AGCAGCATCATGAAGTGTGACACTGACATCCAGAATAAACTTTATGCAGACATTGTACTC
TCCGGGGGCACCACTCTCCTCCCTGGGCTGGAGGAAAGGCTCATGAAGGAAGTGGAACAG
CTGGCTTCCAAAGGTACTCCCATCAAGATCACAGCTTCTCCTGATAGATGCTTCTCTGCA
TGGATTGGTGCATCCATCATGACCTCTATGAGCAGTTTCAAGCAGATGTGGGTCACCTCG
GCAGACTTCAAGGAGTATGGGACATCTGTGGTTCAAAGAAGGTGCTTT


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: ''
Sequencing Primer: Forward:  CMV forward
Reverse:  None
Description: with tag/fusion/marker assay, Gateway®, acceptor (destination), recombinational cloning clone, lentiviral infection expression with BlastR and T7 and attR1 and CMV and cPPT and V5 and AmpR and attR2 and CAT and ccdB and WPRE; ampicillin resistance in bacterial, blasticidin resistance in mammalian;
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: EvNO00877063
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter CMV CMV promoter 14 566
promoter T7 T7 promoter 3690 3672
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker BlastR Blasticidin resistance gene 8753 9148
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ACTRT1
Symbol Nomenclature : ACTRT1
SYNONYM : AIP1; ARIP1; ARPT1; HSD27
Full Nomenclature : actin-related protein T1
GENEID : 139741
Locus Tag : RP1-203C2.1
GenBank Accession : EU832268
HGNC : 24027
MIM : 300487
Vega : OTTHUMG00000022359
Target GenBank: EU832268

 

Reference Sequence Alignment

  

HsCD00438152      ATGTTTAATCCACATGCATTAGATGTTCCTGCTGTAATTTTTGACAATGGTTCAGGACTC
NM_138289.3       ATGTTTAATCCACATGCATTAGATGTTCCTGCTGTAATTTTTGACAATGGTTCAGGACTC
                  ************************************************************

HsCD00438152      TGCAAAGCAGGCCTGTCTGGAGAGATTGGACCCCGCCATGTCATCAGCTCCGTCTTGGGA
NM_138289.3       TGCAAAGCAGGCCTGTCTGGAGAGATTGGACCCCGCCATGTCATCAGCTCCGTCTTGGGA
                  ************************************************************

HsCD00438152      CATTGTAAATTCAATGTGCCTTTAGCAAGACTTAATCAGAAGTACTTCGTGGGGCAAGAA
NM_138289.3       CATTGTAAATTCAATGTGCCTTTAGCAAGACTTAATCAGAAGTACTTCGTGGGGCAAGAA
                  ************************************************************

HsCD00438152      GCCCTGTACAAGTATGAGGCCCTACATTTGCACTACCCCATTGAGCGTGGACTGGTAACA
NM_138289.3       GCCCTGTACAAGTATGAGGCCCTACATTTGCACTACCCCATTGAGCGTGGACTGGTAACA
                  ************************************************************

HsCD00438152      GGATGGGATGACATGGAGAAACTCTGGAAACATCTCTTTGAGCGGGAGCTTGGAGTAAAA
NM_138289.3       GGATGGGATGACATGGAGAAACTCTGGAAACATCTCTTTGAGCGGGAGCTTGGAGTAAAA
                  ************************************************************

HsCD00438152      CCCAGCCAACAGCCTGTACTTATGACCGAGCCCTCTTTGAATCCTAGGGAAATTCGAGAA
NM_138289.3       CCCAGCCAACAGCCTGTACTTATGACCGAGCCCTCTTTGAATCCTAGGGAAATTCGAGAA
                  ************************************************************

HsCD00438152      AAGCTAGCAGAAATGATGTTTGAGACCTTCAGTGTGCCTGGTTTCTACCTGTCTAATCAT
NM_138289.3       AAGCTAGCAGAAATGATGTTTGAGACCTTCAGTGTGCCTGGTTTCTACCTGTCTAATCAT
                  ************************************************************

HsCD00438152      GCGGTGGCAGCGCTCTATGCCTCTGCCTGTGTCACAGGCCTGGTGGTGGACAGTGGAGAT
NM_138289.3       GCGGTGGCAGCGCTCTATGCCTCTGCCTGTGTCACAGGCCTGGTGGTGGACAGTGGAGAT
                  ************************************************************

HsCD00438152      GGGGTCACTTGCACTGTCCCCATCTTTGAGGGTTACTCCCTGCCTCACGCAGTCACCAAA
NM_138289.3       GGGGTCACTTGCACTGTCCCCATCTTTGAGGGTTACTCCCTGCCTCACGCAGTCACCAAA
                  ************************************************************

HsCD00438152      CTCTGTATGGCAGGGAGGGACATCACAGAGCACCTCACCCGGCTCCTCTTTGCTAGCGGG
NM_138289.3       CTCTGTATGGCAGGGAGGGACATCACAGAGCACCTCACCCGGCTCCTCTTTGCTAGCGGG
                  ************************************************************

HsCD00438152      TTTAACTTCCCTTGCATACTCAACAAGGCCGTGGTAAATAACATCAAAGAGAAGTTGTGC
NM_138289.3       TTTAACTTCCCTTGCATACTCAACAAGGCCGTGGTAAATAACATCAAAGAGAAGTTGTGC
                  ************************************************************

HsCD00438152      TACATCGCCTTGGAGCCAGAGAAAGAGCTACGCAAGAGCCGGGGAGAGGTCCTGGGAGCA
NM_138289.3       TACATCGCCTTGGAGCCAGAGAAAGAGCTACGCAAGAGCCGGGGAGAGGTCCTGGGAGCA
                  ************************************************************

HsCD00438152      TACAGACTGCCAGATGGACATGTCATCCACTTTGGGGATGAGCTGTACCAAGTGCCCGAG
NM_138289.3       TACAGACTGCCAGATGGACATGTCATCCACTTTGGGGATGAGCTGTACCAAGTGCCCGAG
                  ************************************************************

HsCD00438152      GTTCTTTTTGCACCTGACCAGCTGGGCATCCACAGCCCAGGACTCTCAAAAATGGTCTCC
NM_138289.3       GTTCTTTTTGCACCTGACCAGCTGGGCATCCACAGCCCAGGACTCTCAAAAATGGTCTCC
                  ************************************************************

HsCD00438152      AGCAGCATCATGAAGTGTGACACTGACATCCAGAATAAACTTTATGCAGACATTGTACTC
NM_138289.3       AGCAGCATCATGAAGTGTGACACTGACATCCAGAATAAACTTTATGCAGACATTGTACTC
                  ************************************************************

HsCD00438152      TCCGGGGGCACCACTCTCCTCCCTGGGCTGGAGGAAAGGCTCATGAAGGAAGTGGAACAG
NM_138289.3       TCCGGGGGCACCACTCTCCTCCCTGGGCTGGAGGAAAGGCTCATGAAGGAAGTGGAACAG
                  ************************************************************

HsCD00438152      CTGGCTTCCAAAGGTACTCCCATCAAGATCACAGCTTCTCCTGATAGATGCTTCTCTGCA
NM_138289.3       CTGGCTTCCAAAGGTACTCCCATCAAGATCACAGCTTCTCCTGATAGATGCTTCTCTGCA
                  ************************************************************

HsCD00438152      TGGATTGGTGCATCCATCATGACCTCTATGAGCAGTTTCAAGCAGATGTGGGTCACCTCG
NM_138289.3       TGGATTGGTGCATCCATCATGACCTCTATGAGCAGTTTCAAGCAGATGTGGGTCACCTCG
                  ************************************************************

HsCD00438152      GCAGACTTCAAGGAGTATGGGACATCTGTGGTTCAAAGAAGGTGCTTT---
NM_138289.3       GCAGACTTCAAGGAGTATGGGACATCTGTGGTTCAAAGAAGGTGCTTTTAA
                  ************************************************   

Protein

SMART domain : ACTIN : Actin
HPRD : 06439

Clone

Original Clone ID : ccsbBroad304_04932

Organism


TAX_ID : 9606
Species Specific ID: 139741

Genome

Chromosome : X
Map Location : Xq25
Ensembl : ENSG00000123165

Reagents

Labome : ACTRT1-antibody