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CYP2W1 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  CYP2W1
Gene Name:  cytochrome P450, family 2, subfamily W, polypeptide 1
Sequence             
Original Clone ID: ccsbBroad304_12109
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00438261

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1303nts         Open reading frame : 1 to 1303
Reference Sequence Alignment

ATGGAGCTCTCAGAACGCTACGGGCCGGTGTTCACCGTGCACCTGGGGCGCCAGAAGACG
GTGGTGCTGACGGGGTTCGAGGCGGTCAAAGAGGCGCTGGCGGGCCCCGGGCAGGAGCTG
GCCGACCGGCCTCCCATCGCCATCTTCCAGCTCATCCAGCGAGGTGGAGGCATCTTCTTC
TCATCTGGGGCGCGCTGGAGGGCTGCCCGCCAGTTCACGGTGCGTGCCCTGCACAGCCTG
GGCGTGGGCCGGGAGCCGGTGGCTGACAAGATTCTGCAGGAGCTGAAATGCCTCTCTGGG
CAGCTGGATGGCTACAGAGGCCGGCCCTTCCCGCTGGCCCTACTGGGCTGGGCTCCCTCC
AATATCACCTTCGCGCTCCTCTTCGGCCGCCGATTTGACTACCGGGACCCCGTGTTTGTG
TCCCTGCTGGGTCTCATCGATGAGGTCATGGTCCTCTTGGGGTCCCCTGGCCTGCAGCTG
TTCAACGTCTACCCATGGCTCGGGGCCCTGCTCCAGCTGCACCGGCCCGTCCTGCGCAAG
ATCGAGGAGGTCCGTGCCATTCTGAGGACCCTCCTGGAGGCGCGGAGGCCCCACGTGTGC
CCGGGGGACCCCGTGTGCAGCTATGTGGACGCCCTGATCCAGCAGGGACAGGGGGATGAC
CCCGAGGGCCTGTTTGCTGAGGCCAACGCGGTGGCCTGCACCCTGGACATGGTCATGGCC
GGGACGGAGACGACCTCGGCCACGCTGCAGTGGGCCGCACTTCTGATGGGCCGGCACCCG
GACGTGCAGGGCCGGGTGCAGGAGGAGCTAGACCGCGTGCTGGGCCCTGGGCGGACTCCC
CGGCTGGAGGACCAGCAGGCTCTGCCCTACACAAGCGCCGTGCTCCACGAGGTGCAGCGG
TTCATCACGCTCCTGCCGCACGTGCCCCGCTGCACCGCGGCCGACACACAGCTGGGCGGC
TTCCTGCTCCCCAAGGGCACGCCCGTGATTCCCCTGCTGACCTCGGTGCTCCTGGATGAG
ACACAGTGGCAGACCCCAGGCCAGTTCAACCCCGGCCATTTCCTGGACGCGAATGGGCAC
TTTGTGAAGCGGGAGGCCTTCCTGCCTTTCTCTGCAGGCCGCCGCGTCTGTGTTGGGGAG
CGCCTGGCCAGGACCGAGCTCTTCCTGCTGTTTGCCGGCCTCCTGCAGAGGTACCGCCTG
CTGCCCCCGCCTGGCGTCAGTCCGGCCTCCCTGGACACCACGCCCGCCCGGGCTTTTACC
ATGAGGCCGAGGGCCCAGGCCCTGTGTGCGGTGCCCAGGCCC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CYP2W1
Symbol Nomenclature : CYP2W1
Designation : CYPIIW1|cytochrome P450 2W1
Full Nomenclature : cytochrome P450, family 2, subfamily W, polypeptide 1
GENEID : 54905
HGNC : 20243
Vega : OTTHUMG00000074071
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00438261        ------------------------------------------------------------
NM_017781.2         ATGGCCCTGCTGCTCTTGCTGTTCCTGGGCCTCCTGGGGCTCTGGGGGCTGCTCTGCGCC
XM_005249792.1      ATGGCCCTGCTGCTCTTGCTGTTCCTGGGCCTCCTGGGGCTCTGGGGGCTGCTCTGCGCC
                                                                                

HsCD00438261        ------------------------------------------------------------
NM_017781.2         TGCGCCCAAGACCCCTCCCCAGCTGCCCGGTGGCCCCCGGGGCCTCGCCCGCTGCCGCTC
XM_005249792.1      TGCGCCCAAGACCCCTCCCCAGCTGCCCGGTGGCCCCCGGGGCCTCGCCCGCTGCCGCTC
                                                                                

HsCD00438261        ------------------------------------------------ATGGAGCTCTCA
NM_017781.2         GTCGGGAACCTGCACTTGCTGCGTCTGTCGCAACAGGACCGGTCCCTGATGGAGCTCTCA
XM_005249792.1      GTCGGGAACCTGCACTTGCTGCGTCTGTCGCAACAGGACCGGTCCCTGATGGAGCTCTCA
                                                                    ************

HsCD00438261        GAACGCTACGGGCCGGTGTTCACCGTGCACCTGGGGCGCCAGAAGACGGTGGTGCTGACG
NM_017781.2         GAACGCTACGGGCCGGTGTTCACCGTGCACCTGGGGCGCCAGAAGACGGTGGTGCTGACG
XM_005249792.1      GAACGCTACGGGCCGGTGTTCACCGTGCACCTGGGGCGCCAGAAGACGGTGGTGCTGACG
                    ************************************************************

HsCD00438261        GGGTTCGAGGCGGTCAAAGAGGCGCTGGCGGGCCCCGGGCAGGAGCTGGCCGACCGGCCT
NM_017781.2         GGGTTCGAGGCGGTCAAAGAGGCGCTGGCGGGCCCCGGGCAGGAGCTGGCCGACCGGCCT
XM_005249792.1      GGGTTCGAGGCGGTCAAAGAGGCGCTGGCGGGCCCCGGGCAGGAGCTGGCCGACCGGCCT
                    ************************************************************

HsCD00438261        CCCATCGCCATCTTCCAGCTCATCCAGCGAGGTGGAGGCATCTTCTTCTCATCTGGGGCG
NM_017781.2         CCCATCGCCATCTTCCAGCTCATCCAGCGAGGTGGAGGCATCTTCTTCTCATCTGGGGCG
XM_005249792.1      CCCATCGCCATCTTCCAGCTCATCCAGCGAGGTGGAGGCATCTTCTTCTCATCTGGGGCG
                    ************************************************************

HsCD00438261        CGCTGGAGGGCTGCCCGCCAGTTCACGGTGCGTGCCCTGCACAGCCTGGGCGTGGGCCGG
NM_017781.2         CGCTGGAGGGCTGCCCGCCAGTTCACGGTGCGTGCCCTGCACAGCCTGGGCGTGGGCCGG
XM_005249792.1      CGCTGGAGGGCTGCCCGCCAGTTCACGGTGCGTGCCCTGCACAGCCTGGGCGTGGGCCGG
                    ************************************************************

HsCD00438261        GAGCCGGTGGCTGACAAGATTCTGCAGGAGCTGAAATGCCTCTCTGGGCAGCTGGATGGC
NM_017781.2         GAGCCGGTGGCTGACAAGATTCTGCAGGAGCTGAAATGCCTCTCTGGGCAGCTGGATGGC
XM_005249792.1      GAGCCGGTGGCTGACAAGATTCTGCAGGAGCTGAAATGCCTCTCTGGGCAGCTGGATGGC
                    ************************************************************

HsCD00438261        TACAGAGGCCGGCCCTTCCCGCTGGCCCTACTGGGCTGGGCTCCCTCCAATATCACCTTC
NM_017781.2         TACAGAGGCCGGCCCTTCCCGCTGGCCCTACTGGGCTGGGCTCCCTCCAATATCACCTTC
XM_005249792.1      TACAGAGGCCGGCCCTTCCCGCTGGCCCTACTGGGCTGGGCTCCCTCCAATATCACCTTC
                    ************************************************************

HsCD00438261        GCGCTCCTCTTCGGCCGCCGATTTGACTACCGGGACCCCGTGTTTGTGTCCCTGCTGGGT
NM_017781.2         GCGCTCCTCTTCGGCCGCCGATTTGACTACCGGGACCCCGTGTTTGTGTCCCTGCTGGGT
XM_005249792.1      GCGCTCCTCTTCGGCCGCCGATTTGACTACCGGGACCCCGTGTTTGTGTCCCTGCTGGGT
                    ************************************************************

HsCD00438261        CTCATCGATGAGGTCATGGTCCTCTTGGGGTCCCCTGGCCTGCAGCTGTTCAACGTCTAC
NM_017781.2         CTCATCGATGAGGTCATGGTCCTCTTGGGGTCCCCTGGCCTGCAGCTGTTCAACGTCTAC
XM_005249792.1      CTCATCGATGAGGTCATGGTCCTCTTGGGGTCCCCTGGCCTGCAGCTGTTCAACGTCTAC
                    ************************************************************

HsCD00438261        CCATGGCTCGGGGCCCTGCTCCAGCTGCACCGGCCCGTCCTGCGCAAGATCGAGGAGGTC
NM_017781.2         CCATGGCTCGGGGCCCTGCTCCAGCTGCACCGGCCCGTCCTGCGCAAGATCGAGGAGGTC
XM_005249792.1      CCATGGCTCGGGGCCCTGCTCCAGCTGCACCGGCCCGTCCTGCGCAAGATCGAGGAGGTC
                    ************************************************************

HsCD00438261        CGTGCCATTCTGAGGACCCTCCTGGAGGCGCGGAGGCCCCACGTGTGCCCGGGGGACCCC
NM_017781.2         CGTGCCATTCTGAGGACCCTCCTGGAGGCGCGGAGGCCCCACGTGTGCCCGGGGGACCCC
XM_005249792.1      CGTGCCATTCTGAGGACCCTCCTGGAGGCGCGGAGGCCCCACGTGTGCCCGGGGGACCCC
                    ************************************************************

HsCD00438261        GTGTGCAGCTATGTGGACGCCCTGATCCAGCAGGGACAGGGGGATGACCCCGAGGGCCTG
NM_017781.2         GTGTGCAGCTATGTGGACGCCCTGATCCAGCAGGGACAGGGGGATGACCCCGAGGGCCTG
XM_005249792.1      GTGTGCAGCTATGTGGACGCCCTGATCCAGCAGGGACAGGGGGATGACCCCGAGGGCCTG
                    ************************************************************

HsCD00438261        TTTGCTGAGGCCAACGCGGTGGCCTGCACCCTGGACATGGTCATGGCCGGGACGGAGACG
NM_017781.2         TTTGCTGAGGCCAACGCGGTGGCCTGCACCCTGGACATGGTCATGGCCGGGACGGAGACG
XM_005249792.1      TTTGCTGAGGCCAACGCGGTGGCCTGCACCCTGGACATGGTCATGGCCGGGACGGAGACG
                    ************************************************************

HsCD00438261        ACCTCGGCCACGCTGCAGTGGGCCGCACTTCTGATGGGCCGGCACCCGGACGTGCAGGGC
NM_017781.2         ACCTCGGCCACGCTGCAGTGGGCCGCACTTCTGATGGGCCGGCACCCGGACGTGCAGGGC
XM_005249792.1      ACCTCGGCCACGCTGCAGTGGGCCGCACTTCTGATGGGCCGGCACCCGGACGTGCAGGGC
                    ************************************************************

HsCD00438261        CGGGTGCAGGAGGAGCTAGACCGCGTGCTGGGCCCTGGGCGGACTCCCCGGCTGGAGGAC
NM_017781.2         CGGGTGCAGGAGGAGCTAGACCGCGTGCTGGGCCCTGGGCGGACTCCCCGGCTGGAGGAC
XM_005249792.1      CGGGTGCAGGAGGAGCTAGACCGCGTGCTGGGCCCTGGGCGGACTCCCCGGCTGGAGGAC
                    ************************************************************

HsCD00438261        CAGCAGGCTCTGCCCTACACAAGCGCCGTGCTCCACGAGGTGCAGCGGTTCATCACGCTC
NM_017781.2         CAGCAGGCTCTGCCCTACACAAGCGCCGTGCTCCACGAGGTGCAGCGGTTCATCACGCTC
XM_005249792.1      CAGCAGGCTCTGCCCTACACAAGCGCCGTGCTCCACGAGGTGCAGCGGTTCATCACGCTC
                    ************************************************************

HsCD00438261        CTGCCGCACGTGCCCCGCTGCACCGCGGCCGACACACAGCTGGGCGGCTTCCTGCTCCCC
NM_017781.2         CTGCCGCACGTGCCCCGCTGCACCGCGGCCGACACACAGCTGGGCGGCTTCCTGCTCCCC
XM_005249792.1      CTGCCGCACGTGCCCCGCTGCACCGCGGCCGACACACAGCTGGGCGGCTTCCTGCTCCCC
                    ************************************************************

HsCD00438261        AAGGGCACGCCCGTGATTCCCCTGCTGACCTCGGTGCTCCTGGATGAGACACAGTGGCAG
NM_017781.2         AAGGGCACGCCCGTGATTCCCCTGCTGACCTCGGTGCTCCTGGATGAGACACAGTGGCAG
XM_005249792.1      AAGGGCACGCCCGTGATTCCCCTGCTGACCTCGGTGCTCCTGGATGAGACACAGTGGCAG
                    ************************************************************

HsCD00438261        ACCCCAGGCCAGTTCAACCCCGGCCATTTCCTGGACGCGAATGGGCACTTTGTGAAGCGG
NM_017781.2         ACCCCAGGCCAGTTCAACCCCGGCCATTTCCTGGACGCGAATGGGCACTTTGTGAAGCGG
XM_005249792.1      ACCCCAGGCCAGTTCAACCCCGGCCATTTCCTGGACGCGAATGGGCACTTTGTGAAGCGG
                    ************************************************************

HsCD00438261        GAGGCCTTCCTGCCTTTCTCTGCAGGCCGCCGCGTCTGTGTTGGGGAGCGCCTGGCCAGG
NM_017781.2         GAGGCCTTCCTGCCTTTCTCTGCAGGCCGCCGCGTCTGTGTTGGGGAGCGCCTGGCCAGG
XM_005249792.1      GAGGCCTTCCTGCCTTTCTCTGCAGGCCGCCGCGTCTGTGTTGGGGAGCGCCTGGCCAGG
                    ************************************************************

HsCD00438261        ACCGAGCTCTTCCTGCTGTTTGCCGGCCTCCTGCAGAGGTACCGCCTGCTGCCCCCGCCT
NM_017781.2         ACCGAGCTCTTCCTGCTGTTTGCCGGCCTCCTGCAGAGGTACCGCCTGCTGCCCCCGCCT
XM_005249792.1      ACCGAGCTCTTCCTGCTGTTTGCCGGCCTCCTGCAGAGGTACCGCCTGCTGCCCCCGCCT
                    ************************************************************

HsCD00438261        GGCGTCAGTCCGGCCTCCCTGGACACCACGCCCGCCCGGGCTTTTACCATGAGGCCGAGG
NM_017781.2         GGCGTCAGTCCGGCCTCCCTGGACACCACGCCCGCCCGGGCTTTTACCATGAGGCCGAGG
XM_005249792.1      GGCGTCAGTCCGGCCTCCCTGGACACCACGCCCGCCCGGGCTTTTACCATGAGGCCGAGG
                    ************************************************************

HsCD00438261        GCCCAGGCCCTGTGTGCGGTGCCCAGGCCC---
NM_017781.2         GCCCAGGCCCTGTGTGCGGTGCCCAGGCCCTAG
XM_005249792.1      GCCCAGGCCCTGTGTGCGGTGCCCAGGCCCTAG
                    ******************************   

Pathways

Reactome : Biological oxidations
Reactome : Cytochrome P450 - arranged by substrate type
Reactome : Metabolism
Reactome : Miscellaneous substrates
Reactome : Phase 1 - Functionalization of compounds
Reactome : Xenobiotics
WikiPathway : cytochrome P450
WikiPathway : metapathway biotransformation

Protein

HPRD : 07901

Clone

Original Clone ID : ccsbBroad304_12109

Organism


TAX_ID : 9606
Species Specific ID: 54905

Genome

Chromosome : 7
Map Location : 7p22.3
Ensembl : ENSG00000073067

Reagents

Labome : CYP2W1-antibody