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ARPM1 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ACTRT3
Gene Name:  actin-related protein T3
Sequence             
Original Clone ID: ccsbBroad304_09196
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00438492

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1117nts        
Open reading frame : 1 to 1117
Reference Sequence Alignment

ATGAACCACTGCCAGCTACCGGTGGTGATCGACAACGGCTCGGGAATGATCAAGGCGGGC
GTGGCTGGGTGCCGGGAGCCCCAGTTTATCTACCCGAACATTATCGGCCGCGCCAAGGGC
CAGAGCCGCGCGGCCCAGGGCGGGCTAGAACTCTGCGTGGGCGACCAAGCTCAGGACTGG
AGGAGCTCGCTGTTCATCAGTTACCCAGTGGAGCGTGGTCTCATTACTTCATGCGAGGAC
ATGGAGATCATGTGGAAGCATATCTATGACTATAACCTAAAGCTGAAGCCGTGTGATGGC
CCAGTCTTGATTACTGAGCCAGCGCTGAACCCACTGGCCAACCGGCAACAGATCACGGAA
ATGTTTTTTGAGCATCTGGGTGTTCCTGCCTTCTATATGTCCATCCAGGCTGTGCTGGCT
CTCTTTGCTGCTGGCTTCACTACTGGCCTTGTGCTGAATTCAGGTGCTGGGGTTACCCAG
AGTGTGCCCATCTTTGAGGGTTACTGTCTGCCTCATGGTGTGCAGCAACTGGATCTGGCA
GGCCTTGACCTCACCAACTACCTCATGGTGCTAATGAAGAACCATGGTATCATGTTGCTC
AGTGCTTCAGACAGAAAGATTGTTGAAGACATCAAGGAGAGCTTTTGTTATGTGGCAATG
AACTACGAAGAGGAAATGGCCAAGAAACCCGATTGTCTAGAGAAAGTTTACCAACTACCT
GATGGGAAGGTCATCCAGCTCCATGACCAGCTCTTTTCTTGTCCAGAGGCCCTCTTCTCT
CCGTGTCATATGAACCTTGAGGCCCCTGGCATTGATAAGATATGCTTCAGCAGCATAATG
AAATGTGATACAGGCCTGAGGAATTCCTTCTTTTCCAATATTATCCTTGCCGGGGGATCA
ACCTCTTTCCCTGGTTTAGACAAGCGGTTAGTTAAGGATATAGCAAAGGTGGCTCCTGCC
AACACCGCTGTGCAAGTTATAGCTCCTCCAGAAAGGAAAATATCAGTGTGGATGGGAGGT
TCTATTCTTGCATCCTTGTCTGCCTTCCAGGACATGTGGATCACTGCTGCAGAATTTAAA
GAAGTTGGACCCAACATAGTACACCAAAGATGCTTC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: ''
Sequencing Primer: Forward:  CMV forward
Reverse:  None
Description: with tag/fusion/marker assay, Gateway®, acceptor (destination), recombinational cloning clone, lentiviral infection expression with BlastR and T7 and attR1 and CMV and cPPT and V5 and AmpR and attR2 and CAT and ccdB and WPRE; ampicillin resistance in bacterial, blasticidin resistance in mammalian;
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: EvNO00877063
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter CMV CMV promoter 14 566
promoter T7 T7 promoter 3690 3672
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker BlastR Blasticidin resistance gene 8753 9148
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ACTRT3
Symbol Nomenclature : ACTRT3
SYNONYM : ARP-T3; ARPM1
Designation : actin related protein M1|actin-related protein M1
Full Nomenclature : actin-related protein T3
GENEID : 84517
GenBank Accession : EU176440
HGNC : 24022
MIM : 608534
Vega : OTTHUMG00000184025
Target GenBank: EU176440

 

Reference Sequence Alignment

  

HsCD00438492      ATGAACCACTGCCAGCTACCGGTGGTGATCGACAACGGCTCGGGAATGATCAAGGCGGGC
NM_032487.4       ATGAACCACTGCCAGCTACCGGTGGTGATCGACAACGGCTCGGGAATGATCAAGGCGGGC
                  ************************************************************

HsCD00438492      GTGGCTGGGTGCCGGGAGCCCCAGTTTATCTACCCGAACATTATCGGCCGCGCCAAGGGC
NM_032487.4       GTGGCTGGGTGCCGGGAGCCCCAGTTTATCTACCCGAACATTATCGGCCGCGCCAAGGGC
                  ************************************************************

HsCD00438492      CAGAGCCGCGCGGCCCAGGGCGGGCTAGAACTCTGCGTGGGCGACCAAGCTCAGGACTGG
NM_032487.4       CAGAGCCGCGCGGCCCAGGGCGGGCTAGAACTCTGCGTGGGCGACCAAGCTCAGGACTGG
                  ************************************************************

HsCD00438492      AGGAGCTCGCTGTTCATCAGTTACCCAGTGGAGCGTGGTCTCATTACTTCATGCGAGGAC
NM_032487.4       AGGAGCTCGCTGTTCATCAGTTACCCAGTGGAGCGTGGTCTCATTACTTCATGGGAGGAC
                  ***************************************************** ******

HsCD00438492      ATGGAGATCATGTGGAAGCATATCTATGACTATAACCTAAAGCTGAAGCCGTGTGATGGC
NM_032487.4       ATGGAGATCATGTGGAAGCATATCTATGACTATAACCTAAAGCTGAAGCCGTGTGATGGC
                  ************************************************************

HsCD00438492      CCAGTCTTGATTACTGAGCCAGCGCTGAACCCACTGGCCAACCGGCAACAGATCACGGAA
NM_032487.4       CCAGTCTTGATTACTGAGCCAGCGCTGAACCCACTGGCCAACCGGCAACAGATCACGGAA
                  ************************************************************

HsCD00438492      ATGTTTTTTGAGCATCTGGGTGTTCCTGCCTTCTATATGTCCATCCAGGCTGTGCTGGCT
NM_032487.4       ATGTTTTTTGAGCATCTGGGTGTTCCTGCCTTCTATATGTCCATCCAGGCTGTGCTGGCT
                  ************************************************************

HsCD00438492      CTCTTTGCTGCTGGCTTCACTACTGGCCTTGTGCTGAATTCAGGTGCTGGGGTTACCCAG
NM_032487.4       CTCTTTGCTGCTGGCTTCACTACTGGCCTTGTGCTGAATTCAGGTGCTGGGGTTACCCAG
                  ************************************************************

HsCD00438492      AGTGTGCCCATCTTTGAGGGTTACTGTCTGCCTCATGGTGTGCAGCAACTGGATCTGGCA
NM_032487.4       AGTGTGCCCATCTTTGAGGGTTACTGTCTGCCTCATGGTGTGCAGCAACTGGATCTGGCA
                  ************************************************************

HsCD00438492      GGCCTTGACCTCACCAACTACCTCATGGTGCTAATGAAGAACCATGGTATCATGTTGCTC
NM_032487.4       GGCCTTGACCTCACCAACTACCTCATGGTGCTAATGAAGAACCATGGTATCATGTTGCTC
                  ************************************************************

HsCD00438492      AGTGCTTCAGACAGAAAGATTGTTGAAGACATCAAGGAGAGCTTTTGTTATGTGGCAATG
NM_032487.4       AGTGCTTCAGACAGAAAGATTGTTGAAGACATCAAGGAGAGCTTTTGTTATGTGGCAATG
                  ************************************************************

HsCD00438492      AACTACGAAGAGGAAATGGCCAAGAAACCCGATTGTCTAGAGAAAGTTTACCAACTACCT
NM_032487.4       AACTACGAAGAGGAAATGGCCAAGAAACCCGATTGTCTAGAGAAAGTTTACCAACTACCT
                  ************************************************************

HsCD00438492      GATGGGAAGGTCATCCAGCTCCATGACCAGCTCTTTTCTTGTCCAGAGGCCCTCTTCTCT
NM_032487.4       GATGGGAAGGTCATCCAGCTCCATGACCAGCTCTTTTCTTGTCCAGAGGCCCTCTTCTCT
                  ************************************************************

HsCD00438492      CCGTGTCATATGAACCTTGAGGCCCCTGGCATTGATAAGATATGCTTCAGCAGCATAATG
NM_032487.4       CCGTGTCATATGAACCTTGAGGCCCCTGGCATTGATAAGATATGCTTCAGCAGCATAATG
                  ************************************************************

HsCD00438492      AAATGTGATACAGGCCTGAGGAATTCCTTCTTTTCCAATATTATCCTTGCCGGGGGATCA
NM_032487.4       AAATGTGATACAGGCCTGAGGAATTCCTTCTTTTCCAATATTATCCTTGCCGGGGGATCA
                  ************************************************************

HsCD00438492      ACCTCTTTCCCTGGTTTAGACAAGCGGTTAGTTAAGGATATAGCAAAGGTGGCTCCTGCC
NM_032487.4       ACCTCTTTCCCTGGTTTAGACAAGCGGTTAGTTAAGGATATAGCAAAGGTGGCTCCTGCC
                  ************************************************************

HsCD00438492      AACACCGCTGTGCAAGTTATAGCTCCTCCAGAAAGGAAAATATCAGTGTGGATGGGAGGT
NM_032487.4       AACACCGCTGTGCAAGTTATAGCTCCTCCAGAAAGGAAAATATCAGTGTGGATGGGAGGT
                  ************************************************************

HsCD00438492      TCTATTCTTGCATCCTTGTCTGCCTTCCAGGACATGTGGATCACTGCTGCAGAATTTAAA
NM_032487.4       TCTATTCTTGCATCCTTGTCTGCCTTCCAGGACATGTGGATCACTGCTGCAGAATTTAAA
                  ************************************************************

HsCD00438492      GAAGTTGGACCCAACATAGTACACCAAAGATGCTTC---
NM_032487.4       GAAGTTGGACCCAACATAGTACACCAAAGATGCTTCTGA
                  ************************************   

Protein

SMART domain : ACTIN : Actin
HPRD : 12253

Clone

Original Clone ID : ccsbBroad304_09196

Organism


TAX_ID : 9606
Species Specific ID: 84517

Genome

Chromosome : 3
Map Location : 3q26.2
Ensembl : ENSG00000184378

Reagents

Labome : ACTRT3-antibody