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ACTL7B (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ACTL7B
Gene Name:  actin-like 7B
Sequence             
Original Clone ID: ccsbBroad304_07698
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00438593

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1246nts        
Open reading frame : 1 to 1246
Reference Sequence Alignment

ATGGCGACAAGGAACAGCCCCATGCCCCTGGGCACGGCTCAGGGTGACCCTGGAGAGGCA
GGAACACGGCCCGGCCCTGACGCCAGCCTCCGGGACACAGGTGCGGCCACTCAGCTCAAG
ATGAAGCCCAGGAAGGTGCACAAGATCAAGGCGGTCATCATCGACCTGGGCTCCCAGTAC
TGCAAGTGCGGCTACGCGGGAGAGCCGAGGCCCACCTACTTCATCTCCTCCACCGTGGGC
AAACGCTGCCCCGAGGCGGCCGACGCTGGCGACACCCGCAAGTGGACTTTAGTGGGCCAT
GAGCTGCTCAACACGGAGGCGCCTCTCAAGCTGGTGAACCCGCTGAAGCACGGCATCGTG
GTGGACTGGGACTGCGTGCAGGACATCTGGGAGTACATCTTCCGCACCGCCATGAAGATC
CTCCCCGAGGAGCACGCTGTGCTGGTCTCCGACCCTCCGCTCAGCCCCAGCAGCAACCGG
GAGAAGTACGCGGAGCTCATGTTTGAGACCTTCGGCATCCCAGCCATGCACGTGACGTCC
CAGTCGTTGCTGTCCATCTACTCCTACGGCAAGACCTCGGGGCTGGTGGTGGAGAGCGGG
CACGGCGTCTCGCACGTGGTGCCCATATCCGAGGGCGACGTGCTGCCGGGCCTGACCAGC
CGCGCCGACTACGCTGGGGGTGACCTCACCAACTACCTGATGCAGCTGCTCAATGAGGCG
GGCCACGCATTCACGGACGACCACCTGCACATCATAGAGCACATCAAGAAGAAGTGCTGC
TATGCGGCCTTCCTGCCCGAGGAGGAGCTGGGCCTGGTCCCGGAGGAGCTGCGCGTGGAC
TACGAGCTCCCGGACGGCAAACTCATCACCATTGGCCAGGAGCGCTTCCGTTGCTCTGAG
ATGCTCTTCCAGCCCTCCCTGGCAGGCAGCACCCAGCCGGGCCTCCCGGAGCTCACAGCT
GCCTGCCTGGGCCGCTGCCAGGACACGGGCTTCAAGGAGGAGATGGCCGCCAACGTGCTA
CTGTGTGGCGGCTGCACTATGCTGGATGGCTTCCCCGAGCGCTTCCAGAGGGAGCTGAGC
CTCCTCTGCCCCGGGGACAGCCCTGCAGTGGCTGCCGCTCCTGAGAGGAAGACCTCCGTG
TGGACCGGCGGTTCCATCCTGGCCTCCCTGCAGGCCTTCCAACAGCTCTGGGTCAGCAAG
GAAGAGTTTGAGGAGCGGGGCAGCGTGGCCATCTACAGCAAGTGC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: ''
Sequencing Primer: Forward:  CMV forward
Reverse:  None
Description: with tag/fusion/marker assay, Gateway®, acceptor (destination), recombinational cloning clone, lentiviral infection expression with BlastR and T7 and attR1 and CMV and cPPT and V5 and AmpR and attR2 and CAT and ccdB and WPRE; ampicillin resistance in bacterial, blasticidin resistance in mammalian;
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: EvNO00877063
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter CMV CMV promoter 14 566
promoter T7 T7 promoter 3690 3672
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker BlastR Blasticidin resistance gene 8753 9148
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ACTL7B
Symbol Nomenclature : ACTL7B
SYNONYM : Tact1
Designation : actin-like 7-beta|actin-like protein 7B|actin-like-7-beta
Full Nomenclature : actin-like 7B
GENEID : 10880
Locus Tag : RP11-3J11.2
HGNC : 162
MIM : 604304
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00438593      ATGGCGACAAGGAACAGCCCCATGCCCCTGGGCACGGCTCAGGGTGACCCTGGAGAGGCA
NM_006686.3       ATGGCGACAAGGAACAGCCCCATGCCCCTGGGCACGGCTCAGGGTGACCCTGGAGAGGCA
                  ************************************************************

HsCD00438593      GGAACACGGCCCGGCCCTGACGCCAGCCTCCGGGACACAGGTGCGGCCACTCAGCTCAAG
NM_006686.3       GGAACACGGCCCGGCCCTGACGCCAGCCTCCGGGACACAGGTGCGGCCACTCAGCTCAAG
                  ************************************************************

HsCD00438593      ATGAAGCCCAGGAAGGTGCACAAGATCAAGGCGGTCATCATCGACCTGGGCTCCCAGTAC
NM_006686.3       ATGAAGCCCAGGAAGGTGCACAAGATCAAGGCGGTCATCATCGACCTGGGCTCCCAGTAC
                  ************************************************************

HsCD00438593      TGCAAGTGCGGCTACGCGGGAGAGCCGAGGCCCACCTACTTCATCTCCTCCACCGTGGGC
NM_006686.3       TGCAAGTGCGGCTACGCGGGAGAGCCGAGGCCCACCTACTTCATCTCCTCCACCGTGGGC
                  ************************************************************

HsCD00438593      AAACGCTGCCCCGAGGCGGCCGACGCTGGCGACACCCGCAAGTGGACTTTAGTGGGCCAT
NM_006686.3       AAACGCTGCCCCGAGGCGGCCGACGCTGGCGACACCCGCAAGTGGACTTTAGTGGGCCAT
                  ************************************************************

HsCD00438593      GAGCTGCTCAACACGGAGGCGCCTCTCAAGCTGGTGAACCCGCTGAAGCACGGCATCGTG
NM_006686.3       GAGCTGCTCAACACGGAGGCGCCTCTCAAGCTGGTGAACCCGCTGAAGCACGGCATCGTG
                  ************************************************************

HsCD00438593      GTGGACTGGGACTGCGTGCAGGACATCTGGGAGTACATCTTCCGCACCGCCATGAAGATC
NM_006686.3       GTGGACTGGGACTGCGTGCAGGACATCTGGGAGTACATCTTCCGCACCGCCATGAAGATC
                  ************************************************************

HsCD00438593      CTCCCCGAGGAGCACGCTGTGCTGGTCTCCGACCCTCCGCTCAGCCCCAGCAGCAACCGG
NM_006686.3       CTCCCCGAGGAGCACGCTGTGCTGGTCTCCGACCCTCCGCTCAGCCCCAGCAGCAACCGG
                  ************************************************************

HsCD00438593      GAGAAGTACGCGGAGCTCATGTTTGAGACCTTCGGCATCCCAGCCATGCACGTGACGTCC
NM_006686.3       GAGAAGTACGCGGAGCTCATGTTTGAGACCTTCGGCATCCCAGCCATGCACGTGACGTCC
                  ************************************************************

HsCD00438593      CAGTCGTTGCTGTCCATCTACTCCTACGGCAAGACCTCGGGGCTGGTGGTGGAGAGCGGG
NM_006686.3       CAGTCGTTGCTGTCCATCTACTCCTACGGCAAGACCTCGGGGCTGGTGGTGGAGAGCGGG
                  ************************************************************

HsCD00438593      CACGGCGTCTCGCACGTGGTGCCCATATCCGAGGGCGACGTGCTGCCGGGCCTGACCAGC
NM_006686.3       CACGGCGTCTCGCACGTGGTGCCCATATCCGAGGGCGACGTGCTGCCGGGCCTGACCAGC
                  ************************************************************

HsCD00438593      CGCGCCGACTACGCTGGGGGTGACCTCACCAACTACCTGATGCAGCTGCTCAATGAGGCG
NM_006686.3       CGCGCCGACTACGCTGGGGGTGACCTCACCAACTACCTGATGCAGCTGCTCAATGAGGCG
                  ************************************************************

HsCD00438593      GGCCACGCATTCACGGACGACCACCTGCACATCATAGAGCACATCAAGAAGAAGTGCTGC
NM_006686.3       GGCCACGCATTCACGGACGACCACCTGCACATCATAGAGCACATCAAGAAGAAGTGCTGC
                  ************************************************************

HsCD00438593      TATGCGGCCTTCCTGCCCGAGGAGGAGCTGGGCCTGGTCCCGGAGGAGCTGCGCGTGGAC
NM_006686.3       TATGCGGCCTTCCTGCCCGAGGAGGAGCTGGGCCTGGTCCCGGAGGAGCTGCGCGTGGAC
                  ************************************************************

HsCD00438593      TACGAGCTCCCGGACGGCAAACTCATCACCATTGGCCAGGAGCGCTTCCGTTGCTCTGAG
NM_006686.3       TACGAGCTCCCGGACGGCAAACTCATCACTATTGGCCAGGAGCGCTTCCGTTGCTCTGAG
                  ***************************** ******************************

HsCD00438593      ATGCTCTTCCAGCCCTCCCTGGCAGGCAGCACCCAGCCGGGCCTCCCGGAGCTCACAGCT
NM_006686.3       ATGCTCTTCCAGCCCTCCCTGGCAGGCAGCACCCAGCCGGGCCTCCCGGAGCTCACAGCT
                  ************************************************************

HsCD00438593      GCCTGCCTGGGCCGCTGCCAGGACACGGGCTTCAAGGAGGAGATGGCCGCCAACGTGCTA
NM_006686.3       GCCTGCCTGGGCCGCTGCCAGGACACGGGCTTCAAGGAGGAGATGGCCGCCAACGTGCTA
                  ************************************************************

HsCD00438593      CTGTGTGGCGGCTGCACTATGCTGGATGGCTTCCCCGAGCGCTTCCAGAGGGAGCTGAGC
NM_006686.3       CTGTGTGGCGGCTGCACTATGCTGGATGGCTTCCCCGAGCGCTTCCAGAGGGAGCTGAGC
                  ************************************************************

HsCD00438593      CTCCTCTGCCCCGGGGACAGCCCTGCAGTGGCTGCCGCTCCTGAGAGGAAGACCTCCGTG
NM_006686.3       CTCCTCTGCCCCGGGGACAGCCCTGCAGTGGCTGCCGCTCCTGAGAGGAAGACCTCCGTG
                  ************************************************************

HsCD00438593      TGGACCGGCGGTTCCATCCTGGCCTCCCTGCAGGCCTTCCAACAGCTCTGGGTCAGCAAG
NM_006686.3       TGGACCGGCGGTTCCATCCTGGCCTCCCTGCAGGCCTTCCAACAGCTCTGGGTCAGCAAG
                  ************************************************************

HsCD00438593      GAAGAGTTTGAGGAGCGGGGCAGCGTGGCCATCTACAGCAAGTGC---
NM_006686.3       GAAGAGTTTGAGGAGCGGGGCAGCGTGGCCATCTACAGCAAGTGCTGA
                  *********************************************   

Protein

SMART domain : ACTIN : Actin
HPRD : 11977

Clone

Original Clone ID : ccsbBroad304_07698

Organism


TAX_ID : 9606
Species Specific ID: 10880

Genome

Chromosome : 9
Map Location : 9q31

Reagents

Labome : ACTL7B-antibody