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BPIFB2 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  BPIFB2
Gene Name:  BPI fold containing family B, member 2
Sequence             
Original Clone ID: ccsbBroad304_09032
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00438621

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1375nts         Open reading frame : 1 to 1375
Reference Sequence Alignment

ATGGCTTGGGCAAGTAGGCTGGGCCTGCTGCTGGCACTGCTGCTGCCCGTGGTCGGTGCC
TCCACGCCAGGCACCGTGGTCCGACTCAACAAGGCAGCATTGAGCTACGTGTCTGAAATT
GGGAAAGCCCCTCTCCAGCGGGCCCTGCAGGTCACTGTCCCTCATTTCCTGGACTGGAGT
GGAGAGGCGCTTCAGCCCACCAGGATCCGGATTCTGAATGTCCATGTGCCCCGCCTCCAC
CTGAAATTCATTGCTGGTTTCGGAGTGCGCCTGCTGGCAGCAGCTAATTTTACTTTCAAG
GTCTTTCGCGCCCCAGAGCCCCTGGAGCTGACGCTGCCTGTGGAACTGCTGGCTGACACC
CGCGTGACCCAGAGCTCCATCAGGACCCCTGTGGTCAGCATCTCTGCCTGCTCTTTATTC
TCGGGCCACGCCAACGAGTTTGATGGCAGTAACAGCACCTCCCACGCGCTGCTGGTCCTG
GTGCAGAAGCACATTAAAGCTGTCTTGAGTAACAAGCTGTGCCTGAGCATCTCCAACCTG
GTGCAGGGTGTCAATGTCCACCTGGGCACCTTAATTGGCCTCAACCCCGTGGGTCCTGAG
TCCCAGATCCGCTATTCCATGGTCAGTGTGCCCACTGTCACCAGTGACTACATTTCCCTG
GAAGTCAATGCTGTTCTCTTCCTGCTGGGCAAGCCCATCATCCTGCCCACGGATGCCACC
CCTTTTGTGTTGCCAAGGCATGTGGGTACCGAGGGCTCCATGGCCACCGGGGGCCTCTCC
CAGCAGCTGTTTGACTCTGCGCTCCTGCTGCTGCAGAAGGCCGGTGCCCTCAACCTGGAC
ATCACAGGGCAGCTGAGGTCGGATGACAACCTGCTGAACACCTCTGCTCTGGGCCGGCTC
ATCCCGGAGGTGGCCCGCCAGTTTCCCGAGCCCATGCCTGTGGTGCTCAAGGTGCGGCTG
GGTGCCACACCTGTGGCCATGCTCCACACAAACAACGCCACCCTGCGGCTGCAGCCCTTC
GTGGAGGTCCTGGCCACAGCCTCCAACTCGGCTTTCCAGTCCCTCTTCTCCCTGGATGTG
GTAGTGAACTTGAGACTCCAGCTCTCTGTGTCCAAGGTGAAGCTTCAGGGGACCACGTCT
GTGCTGGGGGATGTCCAGCTCACGGTGGCCTCCTCCAACGTGGGCTTCATTGATACAGAT
CAGGTGCGCACACTGATGGGCACCGTTTTTGAGAAGCCCCTGCTGGACCATCTCAATGCT
CTCTTGGCCATGGGAATTGCCCTCCCTGGTGTGGTCAACCTCCACTATGTCGCCCCTGAG
ATCTTTGTCTATGAGGGCTACGTGGTGATATCCAGTGGACACTTCTACCAGAGC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
1 error; 1 non-synonomous errors
                                

5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : BPIFB2
Symbol Nomenclature : BPIFB2
SYNONYM : BPIL1; C20orf184; LPLUNC2; RYSR; dJ726C3.2
Designation : BPI fold-containing family B member 2|BPI-like 1|bactericidal/permeability-increasing protein-like 1|long palate, lung and nasal epithelium carcinoma-associated protein 2
Full Nomenclature : BPI fold containing family B, member 2
GENEID : 80341
Locus Tag : RP4-726C3.7
HGNC : 16177
MIM : 614108
Vega : OTTHUMG00000032232
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00438621      ATGGCTTGGGCAAGTAGGCTGGGCCTGCTGCTGGCACTGCTGCTGCCCGTGGTCGGTGCC
NM_025227.2       ATGGCTTGGGCAAGTAGGCTGGGCCTGCTGCTGGCACTGCTGCTGCCCGTGGTCGGTGCC
                  ************************************************************

HsCD00438621      TCCACGCCAGGCACCGTGGTCCGACTCAACAAGGCAGCATTGAGCTACGTGTCTGAAATT
NM_025227.2       TCCACGCCAGGCACCGTGGTCCGACTCAACAAGGCAGCATTGAGCTACGTGTCTGAAATT
                  ************************************************************

HsCD00438621      GGGAAAGCCCCTCTCCAGCGGGCCCTGCAGGTCACTGTCCCTCATTTCCTGGACTGGAGT
NM_025227.2       GGGAAAGCCCCTCTCCAGCGGGCCCTGCAGGTCACTGTCCCTCATTTCCTGGACTGGAGT
                  ************************************************************

HsCD00438621      GGAGAGGCGCTTCAGCCCACCAGGATCCGGATTCTGAATGTCCATGTGCCCCGCCTCCAC
NM_025227.2       GGAGAGGCGCTTCAGCCCACCAGGATCCGGATTCTGAATGTCCATGTGCCCCGCCTCCAC
                  ************************************************************

HsCD00438621      CTGAAATTCATTGCTGGTTTCGGAGTGCGCCTGCTGGCAGCAGCTAATTTTACTTTCAAG
NM_025227.2       CTGAAATTCATTGCTGGTTTCGGAGTGCGCCTGCTGGCAGCAGCTAATTTTACTTTCAAG
                  ************************************************************

HsCD00438621      GTCTTTCGCGCCCCAGAGCCCCTGGAGCTGACGCTGCCTGTGGAACTGCTGGCTGACACC
NM_025227.2       GTCTTTCGCGCCCCAGAGCCCCTGGAGCTGACGCTGCCTGTGGAACTGCTGGCTGACACC
                  ************************************************************

HsCD00438621      CGCGTGACCCAGAGCTCCATCAGGACCCCTGTGGTCAGCATCTCTGCCTGCTCTTTATTC
NM_025227.2       CGCGTGACCCAGAGCTCCATCAGGACCCCTGTGGTCAGCATCTCTGCCTGCTCTTTATTC
                  ************************************************************

HsCD00438621      TCGGGCCACGCCAACGAGTTTGATGGCAGTAACAGCACCTCCCACGCGCTGCTGGTCCTG
NM_025227.2       TCGGGCCACGCCAACGAGTTTGATGGCAGTAACAGCACCTCCCACGCGCTGCTGGTCCTG
                  ************************************************************

HsCD00438621      GTGCAGAAGCACATTAAAGCTGTCTTGAGTAACAAGCTGTGCCTGAGCATCTCCAACCTG
NM_025227.2       GTGCAGAAGCACATTAAAGCTGTCTTGAGTAACAAGCTGTGCCTGAGCATCTCCAACCTG
                  ************************************************************

HsCD00438621      GTGCAGGGTGTCAATGTCCACCTGGGCACCTTAATTGGCCTCAACCCCGTGGGTCCTGAG
NM_025227.2       GTGCAGGGTGTCAATGTCCACCTGGGCACCTTAATTGGCCTCAACCCCGTGGGTCCTGAG
                  ************************************************************

HsCD00438621      TCCCAGATCCGCTATTCCATGGTCAGTGTGCCCACTGTCACCAGTGACTACATTTCCCTG
NM_025227.2       TCCCAGATCCGCTATTCCATGGTCAGTGTGCCCACTGTCACCAGTGACTACATTTCCCTG
                  ************************************************************

HsCD00438621      GAAGTCAATGCTGTTCTCTTCCTGCTGGGCAAGCCCATCATCCTGCCCACGGATGCCACC
NM_025227.2       GAAGTCAATGCTGTTCTCTTCCTGCTGGGCAAGCCCATCATCCTGCCCACGGATGCCACC
                  ************************************************************

HsCD00438621      CCTTTTGTGTTGCCAAGGCATGTGGGTACCGAGGGCTCCATGGCCACCGGGGGCCTCTCC
NM_025227.2       CCTTTTGTGTTGCCAAGGCATGTGGGTACCGAGGGCTCCATGGCCACCGTGGGCCTCTCC
                  ************************************************* **********

HsCD00438621      CAGCAGCTGTTTGACTCTGCGCTCCTGCTGCTGCAGAAGGCCGGTGCCCTCAACCTGGAC
NM_025227.2       CAGCAGCTGTTTGACTCTGCGCTCCTGCTGCTGCAGAAGGCCGGTGCCCTCAACCTGGAC
                  ************************************************************

HsCD00438621      ATCACAGGGCAGCTGAGGTCGGATGACAACCTGCTGAACACCTCTGCTCTGGGCCGGCTC
NM_025227.2       ATCACAGGGCAGCTGAGGTCGGATGACAACCTGCTGAACACCTCTGCTCTGGGCCGGCTC
                  ************************************************************

HsCD00438621      ATCCCGGAGGTGGCCCGCCAGTTTCCCGAGCCCATGCCTGTGGTGCTCAAGGTGCGGCTG
NM_025227.2       ATCCCGGAGGTGGCCCGCCAGTTTCCCGAGCCCATGCCTGTGGTGCTCAAGGTGCGGCTG
                  ************************************************************

HsCD00438621      GGTGCCACACCTGTGGCCATGCTCCACACAAACAACGCCACCCTGCGGCTGCAGCCCTTC
NM_025227.2       GGTGCCACACCTGTGGCCATGCTCCACACAAACAACGCCACCCTGCGGCTGCAGCCCTTC
                  ************************************************************

HsCD00438621      GTGGAGGTCCTGGCCACAGCCTCCAACTCGGCTTTCCAGTCCCTCTTCTCCCTGGATGTG
NM_025227.2       GTGGAGGTCCTGGCCACAGCCTCCAACTCGGCTTTCCAGTCCCTCTTCTCCCTGGATGTG
                  ************************************************************

HsCD00438621      GTAGTGAACTTGAGACTCCAGCTCTCTGTGTCCAAGGTGAAGCTTCAGGGGACCACGTCT
NM_025227.2       GTAGTGAACTTGAGACTCCAGCTCTCTGTGTCCAAGGTGAAGCTTCAGGGGACCACGTCT
                  ************************************************************

HsCD00438621      GTGCTGGGGGATGTCCAGCTCACGGTGGCCTCCTCCAACGTGGGCTTCATTGATACAGAT
NM_025227.2       GTGCTGGGGGATGTCCAGCTCACGGTGGCCTCCTCCAACGTGGGCTTCATTGATACAGAT
                  ************************************************************

HsCD00438621      CAGGTGCGCACACTGATGGGCACCGTTTTTGAGAAGCCCCTGCTGGACCATCTCAATGCT
NM_025227.2       CAGGTGCGCACACTGATGGGCACCGTTTTTGAGAAGCCCCTGCTGGACCATCTCAATGCT
                  ************************************************************

HsCD00438621      CTCTTGGCCATGGGAATTGCCCTCCCTGGTGTGGTCAACCTCCACTATGTCGCCCCTGAG
NM_025227.2       CTCTTGGCCATGGGAATTGCCCTCCCTGGTGTGGTCAACCTCCACTATGTCGCCCCTGAG
                  ************************************************************

HsCD00438621      ATCTTTGTCTATGAGGGCTACGTGGTGATATCCAGTGGACACTTCTACCAGAGC---
NM_025227.2       ATCTTTGTCTATGAGGGCTACGTGGTGATATCCAGTGGACTCTTCTACCAGAGCTGA
                  ****************************************:*************   

Protein

SMART domain : BPI2 : BPI/LBP/CETP C-terminal domain
HPRD : 10690

Clone

Original Clone ID : ccsbBroad304_09032

Organism


TAX_ID : 9606
Species Specific ID: 80341

Genome

Chromosome : 20
Map Location : 20q11.21
Ensembl : ENSG00000078898

Reagents

Labome : BPIFB2-antibody