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GPC4 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  GPC4
Gene Name:  glypican 4
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH180143.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00043892

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1671nts         Open reading frame : 1 to 1671
Reference Sequence Alignment

ATGGCACGGTTCGGCTTGCCCGCGCTTCTCTGCACCCTGGCAGTGCTCAGCGCCGCGCTG
CTGGCTGCCGAGCTCAAGTCGAAAAGTTGCTCGGAAGTGCGACGTCTTTACGTGTCCAAA
GGCTTCAACAAGAACGATGCCCCCCTCCACGAGATCAACGGTGATCATTTGAAGATCTGT
CCCCAGGGTTCTACCTGCTGCTCTCAAGAGATGGAGGAGAAGTACAGCCTGCAAAGTAAA
GATGATTTCAAAAGTGTGGTCAGCGAACAGTGCAATCATTTGCAAGCTGTCTTTGCTTCA
CGTTACAAGAAGTTTGATGAATTCTTCAAAGAACTACTTGAAAATGCAGAGAAATCCCTG
AATGATATGTTTGTGAAGACATATGGCCATTTATACATGCAAAATTCTGAGCTATTTAAA
GATCTCTTCGTAGAGTTGAAACGTTACTACGTGGTGGGAAATGTGAACCTGGAAGAAATG
CTAAATGACTTCTGGGCTCGCCTCCTGGAGCGGATGTTCCGCCTGGTGAACTCCCAGTAC
CACTTTACAGATGAGTATCTGGAATGTGTGAGCAAGTATACGGAGCAGCTGAAGCCCTTC
GGAGATGTCCCTCGCAAATTGAAGCTCCAGGTTACTCGTGCTTTTGTAGCAGCCCGTACT
TTCGCTCAAGGCTTAGCGGTTGCGGGAGATGTCGTGAGCAAGGTCTCCGTGGTAAACCCC
ACAGCCCAGTGTACCCATGCCCTGTTGAAGATGATCTACTGCTCCCACTGCCGGGGTCTC
GTGACTGTGAAGCCATGTTACAACTACTGCTCAAACATCATGAGAGGCTGTTTGGCCAAC
CAAGGGGATCTCGATTTTGAATGGAACAATTTCATAGATGCTATGCTGATGGTGGCAGAG
AGGCTAGAGGGTCCTTTCAACATTGAATCGGTCATGGATCCCATCGATGTGAAGATTTCT
GATGCTATTATGAACATGCAGGATAATAGTGTTCAAGTGTCTCAGAAGGTTTTCCAGGGA
TGTGGACCCCCCAAGCCCCTCCCAGCTGGACGAATTTCTCGTTCCATCTCTGAAAGTGCC
TTCAGTGCTCGCTTCAGACCACATCACCCCGAGGAACGCCCAACCACAGCAGCTGGCACT
AGTTTGGACCGACTGGTTACTGATGTCAAGGAGAAACTGAAACAGGCCAAGAAATTCTGG
TCCTCCCTTCCGAGCAACGTTTGCAACGATGAGAGGATGGCTGCAGGAAACGGCAATGAG
GATGACTGTTGGAATGGGAAAGGCAAAAGCAGGTACCTGTTTGCAGTGACAGGAAATGGA
TTAGCCAACCAGGGCAACAACCCAGAGGTCCAGGTTGACACCAGCAAACCAGACATACTG
ATCCTTCGTCAAATCATGGCTCTTCGAGTGATGACCAGCAAGATGAAGAATGCATACAAT
GGGAACGACGTGGACTTCTTTGATATCAGTGATGAAAGTAGTGGAGAAGGAAGTGGAAGT
GGCTGTGAGTATCAGCAGTGCCCTTCAGAGTTTGACTACAATGCCACTGACCATGCTGGG
AAGAGTGCCAATGAGAAAGCCGACAGTGCTGGTGTCCGTCCTGGGGCACAGGCCTACCTC
CTCACTGTCTTCTGCATCTTGTTCCTGGTTATGCAGAGAGAGTGGAGATAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 2177
Start on reference sequence 507
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : GPC4
Symbol Nomenclature : GPC4
SYNONYM : K-glypican
Designation : dJ900E8.1 (glypican 4)|glypican proteoglycan 4|glypican-4
Full Nomenclature : glypican 4
GENEID : 2239
Locus Tag : RP6-198C21.1
HGNC : 4452
MIM : 300168
Target GenBank: BC017166

 

Reference Sequence Alignment

  

HsCD00043892      ATGGCACGGTTCGGCTTGCCCGCGCTTCTCTGCACCCTGGCAGTGCTCAGCGCCGCGCTG
NM_001448.2       ATGGCACGGTTCGGCTTGCCCGCGCTTCTCTGCACCCTGGCAGTGCTCAGCGCCGCGCTG
                  ************************************************************

HsCD00043892      CTGGCTGCCGAGCTCAAGTCGAAAAGTTGCTCGGAAGTGCGACGTCTTTACGTGTCCAAA
NM_001448.2       CTGGCTGCCGAGCTCAAGTCGAAAAGTTGCTCGGAAGTGCGACGTCTTTACGTGTCCAAA
                  ************************************************************

HsCD00043892      GGCTTCAACAAGAACGATGCCCCCCTCCACGAGATCAACGGTGATCATTTGAAGATCTGT
NM_001448.2       GGCTTCAACAAGAACGATGCCCCCCTCCACGAGATCAACGGTGATCATTTGAAGATCTGT
                  ************************************************************

HsCD00043892      CCCCAGGGTTCTACCTGCTGCTCTCAAGAGATGGAGGAGAAGTACAGCCTGCAAAGTAAA
NM_001448.2       CCCCAGGGTTCTACCTGCTGCTCTCAAGAGATGGAGGAGAAGTACAGCCTGCAAAGTAAA
                  ************************************************************

HsCD00043892      GATGATTTCAAAAGTGTGGTCAGCGAACAGTGCAATCATTTGCAAGCTGTCTTTGCTTCA
NM_001448.2       GATGATTTCAAAAGTGTGGTCAGCGAACAGTGCAATCATTTGCAAGCTGTCTTTGCTTCA
                  ************************************************************

HsCD00043892      CGTTACAAGAAGTTTGATGAATTCTTCAAAGAACTACTTGAAAATGCAGAGAAATCCCTG
NM_001448.2       CGTTACAAGAAGTTTGATGAATTCTTCAAAGAACTACTTGAAAATGCAGAGAAATCCCTG
                  ************************************************************

HsCD00043892      AATGATATGTTTGTGAAGACATATGGCCATTTATACATGCAAAATTCTGAGCTATTTAAA
NM_001448.2       AATGATATGTTTGTGAAGACATATGGCCATTTATACATGCAAAATTCTGAGCTATTTAAA
                  ************************************************************

HsCD00043892      GATCTCTTCGTAGAGTTGAAACGTTACTACGTGGTGGGAAATGTGAACCTGGAAGAAATG
NM_001448.2       GATCTCTTCGTAGAGTTGAAACGTTACTACGTGGTGGGAAATGTGAACCTGGAAGAAATG
                  ************************************************************

HsCD00043892      CTAAATGACTTCTGGGCTCGCCTCCTGGAGCGGATGTTCCGCCTGGTGAACTCCCAGTAC
NM_001448.2       CTAAATGACTTCTGGGCTCGCCTCCTGGAGCGGATGTTCCGCCTGGTGAACTCCCAGTAC
                  ************************************************************

HsCD00043892      CACTTTACAGATGAGTATCTGGAATGTGTGAGCAAGTATACGGAGCAGCTGAAGCCCTTC
NM_001448.2       CACTTTACAGATGAGTATCTGGAATGTGTGAGCAAGTATACGGAGCAGCTGAAGCCCTTC
                  ************************************************************

HsCD00043892      GGAGATGTCCCTCGCAAATTGAAGCTCCAGGTTACTCGTGCTTTTGTAGCAGCCCGTACT
NM_001448.2       GGAGATGTCCCTCGCAAATTGAAGCTCCAGGTTACTCGTGCTTTTGTAGCAGCCCGTACT
                  ************************************************************

HsCD00043892      TTCGCTCAAGGCTTAGCGGTTGCGGGAGATGTCGTGAGCAAGGTCTCCGTGGTAAACCCC
NM_001448.2       TTCGCTCAAGGCTTAGCGGTTGCGGGAGATGTCGTGAGCAAGGTCTCCGTGGTAAACCCC
                  ************************************************************

HsCD00043892      ACAGCCCAGTGTACCCATGCCCTGTTGAAGATGATCTACTGCTCCCACTGCCGGGGTCTC
NM_001448.2       ACAGCCCAGTGTACCCATGCCCTGTTGAAGATGATCTACTGCTCCCACTGCCGGGGTCTC
                  ************************************************************

HsCD00043892      GTGACTGTGAAGCCATGTTACAACTACTGCTCAAACATCATGAGAGGCTGTTTGGCCAAC
NM_001448.2       GTGACTGTGAAGCCATGTTACAACTACTGCTCAAACATCATGAGAGGCTGTTTGGCCAAC
                  ************************************************************

HsCD00043892      CAAGGGGATCTCGATTTTGAATGGAACAATTTCATAGATGCTATGCTGATGGTGGCAGAG
NM_001448.2       CAAGGGGATCTCGATTTTGAATGGAACAATTTCATAGATGCTATGCTGATGGTGGCAGAG
                  ************************************************************

HsCD00043892      AGGCTAGAGGGTCCTTTCAACATTGAATCGGTCATGGATCCCATCGATGTGAAGATTTCT
NM_001448.2       AGGCTAGAGGGTCCTTTCAACATTGAATCGGTCATGGATCCCATCGATGTGAAGATTTCT
                  ************************************************************

HsCD00043892      GATGCTATTATGAACATGCAGGATAATAGTGTTCAAGTGTCTCAGAAGGTTTTCCAGGGA
NM_001448.2       GATGCTATTATGAACATGCAGGATAATAGTGTTCAAGTGTCTCAGAAGGTTTTCCAGGGA
                  ************************************************************

HsCD00043892      TGTGGACCCCCCAAGCCCCTCCCAGCTGGACGAATTTCTCGTTCCATCTCTGAAAGTGCC
NM_001448.2       TGTGGACCCCCCAAGCCCCTCCCAGCTGGACGAATTTCTCGTTCCATCTCTGAAAGTGCC
                  ************************************************************

HsCD00043892      TTCAGTGCTCGCTTCAGACCACATCACCCCGAGGAACGCCCAACCACAGCAGCTGGCACT
NM_001448.2       TTCAGTGCTCGCTTCAGACCACATCACCCCGAGGAACGCCCAACCACAGCAGCTGGCACT
                  ************************************************************

HsCD00043892      AGTTTGGACCGACTGGTTACTGATGTCAAGGAGAAACTGAAACAGGCCAAGAAATTCTGG
NM_001448.2       AGTTTGGACCGACTGGTTACTGATGTCAAGGAGAAACTGAAACAGGCCAAGAAATTCTGG
                  ************************************************************

HsCD00043892      TCCTCCCTTCCGAGCAACGTTTGCAACGATGAGAGGATGGCTGCAGGAAACGGCAATGAG
NM_001448.2       TCCTCCCTTCCGAGCAACGTTTGCAACGATGAGAGGATGGCTGCAGGAAACGGCAATGAG
                  ************************************************************

HsCD00043892      GATGACTGTTGGAATGGGAAAGGCAAAAGCAGGTACCTGTTTGCAGTGACAGGAAATGGA
NM_001448.2       GATGACTGTTGGAATGGGAAAGGCAAAAGCAGGTACCTGTTTGCAGTGACAGGAAATGGA
                  ************************************************************

HsCD00043892      TTAGCCAACCAGGGCAACAACCCAGAGGTCCAGGTTGACACCAGCAAACCAGACATACTG
NM_001448.2       TTAGCCAACCAGGGCAACAACCCAGAGGTCCAGGTTGACACCAGCAAACCAGACATACTG
                  ************************************************************

HsCD00043892      ATCCTTCGTCAAATCATGGCTCTTCGAGTGATGACCAGCAAGATGAAGAATGCATACAAT
NM_001448.2       ATCCTTCGTCAAATCATGGCTCTTCGAGTGATGACCAGCAAGATGAAGAATGCATACAAT
                  ************************************************************

HsCD00043892      GGGAACGACGTGGACTTCTTTGATATCAGTGATGAAAGTAGTGGAGAAGGAAGTGGAAGT
NM_001448.2       GGGAACGACGTGGACTTCTTTGATATCAGTGATGAAAGTAGTGGAGAAGGAAGTGGAAGT
                  ************************************************************

HsCD00043892      GGCTGTGAGTATCAGCAGTGCCCTTCAGAGTTTGACTACAATGCCACTGACCATGCTGGG
NM_001448.2       GGCTGTGAGTATCAGCAGTGCCCTTCAGAGTTTGACTACAATGCCACTGACCATGCTGGG
                  ************************************************************

HsCD00043892      AAGAGTGCCAATGAGAAAGCCGACAGTGCTGGTGTCCGTCCTGGGGCACAGGCCTACCTC
NM_001448.2       AAGAGTGCCAATGAGAAAGCCGACAGTGCTGGTGTCCGTCCTGGGGCACAGGCCTACCTC
                  ************************************************************

HsCD00043892      CTCACTGTCTTCTGCATCTTGTTCCTGGTTATGCAGAGAGAGTGGAGATAG
NM_001448.2       CTCACTGTCTTCTGCATCTTGTTCCTGGTTATGCAGAGAGAGTGGAGATAA
                  **************************************************.

Pathways

Reactome : A tetrasaccharide linker sequence is required for GAG synthesis
Reactome : Chondroitin sulfate/dermatan sulfate metabolism
Reactome : Disease
Reactome : Diseases associated with visual transduction
Reactome : Glycogen storage diseases
Reactome : Glycosaminoglycan metabolism
Reactome : HS-GAG biosynthesis
Reactome : HS-GAG degradation
Reactome : Heparan sulfate/heparin (HS-GAG) metabolism
Reactome : MPS I - Hurler syndrome
Reactome : MPS II - Hunter syndrome
Reactome : MPS IIIA - Sanfilippo syndrome A
Reactome : MPS IIIB - Sanfilippo syndrome B
Reactome : MPS IIIC - Sanfilippo syndrome C
Reactome : MPS IIID - Sanfilippo syndrome D
Reactome : MPS IV - Morquio syndrome A
Reactome : MPS IV - Morquio syndrome B
Reactome : MPS IX - Natowicz syndrome
Reactome : MPS VI - Maroteaux-Lamy syndrome
Reactome : MPS VII - Sly syndrome
Reactome : Metabolism
Reactome : Metabolism of carbohydrates
Reactome : Mucopolysaccharidoses
Reactome : Myoclonic epilepsy of Lafora
Reactome : Retinoid metabolism and transport
Reactome : Signal Transduction
Reactome : Visual phototransduction

Protein

UniProt : O75487
HPRD : 02160

Clone

Cloning Information : 180143
HIP Master Clone ID : 155244
Original Clone ID : FLH180143.01X

Organism


TAX_ID : 9606
Species Specific ID: 2239

Genome

Chromosome : X
Map Location : Xq26.1

Reagents

Labome : GPC4-antibody