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CRISPLD2 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  CRISPLD2
Gene Name:  cysteine-rich secretory protein LCCL domain containing 2
Sequence             
Original Clone ID: ccsbBroad304_12752
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00438942

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1348nts         Open reading frame : 1 to 1348
Reference Sequence Alignment

ATGAGCTGCGTCCTGGGTGGTGTCATCCCCTTGGGGCTGCTGTTCCTGGTCTGCGGATCC
CAAGGCTACCTCCTGCCCAACGTCACTCTCTTAGAGGAGCTGCTCAGCAAATACCAGCAC
AACGAGTCTCACTCCCGGGTCCGCAGAGCCATCCCCAGGGAGGACAAGGAGGAGATCCTC
ATGCTGCACAACAAGCTTCGGGGCCAGGTGCAGCCTCAGGCCTCCAACATGGAGTACATG
ACCTGGGATGACGAACTGGAGAAGTCTGCTGCAGCGTGGGCCAGTCAGTGCATCTGGGAG
CACGGGCCCACCGGTCTGCTGGTGTCCATCGGGCAGAACCTGGGCGCTCACTGGGGCAGG
TATCGCTCTCCGGGGTTCCATGTGCAGTCCTGGTATGACGAGGTGAAGGACTACACCTAC
CCCTACCCGAGCGAGTGCAACCCCTGGTGTCCAGAGAGGTGCTCGGGGCCTATGTGCACG
CACTACACACAGATAGTTTGGGCCACCACCAACAAGATCGGTTGTGCTGTGAACACCTGC
CGGAAGATGACTGTCTGGGGAGAAGTTTGGGAGAACGCGGTCTACTTTGTCTGCAATTAT
TCTCCAAAGGGGAACTGGATTGGAGAAGCCCCCTACAAGAATGGCCGGCCCTGCTCTGAG
TGCCCACCCAGCTATGGAGGCAGCTGCAGGAACAACTTGTGTTACCGAGAAGAAACCTAC
ACTCCAAAACCTGAAACGGACGAGATGAATGAGGTGGAAACGGCTCCCATTCCTGAAGAA
AACCATGTTTGGCTCCAACCGAGGGTGATGAGACCCACCAAGCCCAAGAAAACCTCTGCG
GTCAACTACATGACCCAAGTCGTCAGATGTGACACCAAGATGAAGGACAGGTGCAAAGGG
TCCACGTGTAACAGGTACCAGTGCCCAGCAGGCTGCCTGAACCACAAGGCGAAGATCTTT
GGAAGTCTGTTCTATGAAAGCTCGTCTAGCATATGCCGCGCCGCCATCCACTACGGGATC
CTGGATGACAAGGGAGGCCTGGTGGATATCACCAGGAACGGGAAGGTCCCCTTCTTCGTG
AAGTCTGAGAGACACGGCGTGCAGTCCCTCAGCAAATACAAACCTTCCAGCTCATTCATG
GTGTCAAAAGTGAAAGTGCAGGATTTGGACTGCTACACGACCGTTGCTCAGCTGTGCCCG
TTTGAAAAGCCAGCAACTCACTGCCCAAGAATCCATTGTCCGGCACACTGCAAAGACGAA
CCTTCCTACTGGGCTCCGGTGTTTGGAACCAACATCTATGCAGATGTGAGTAGGATGCAT
TTTCAACAACTATCTCGCAAAGCCTGT


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  None
Description: with tag/fusion/marker assay, Gateway®, acceptor (destination), recombinational cloning clone, lentiviral infection expression with BlastR and T7 and attR1 and CMV and cPPT and V5 and AmpR and attR2 and CAT and ccdB and WPRE; ampicillin resistance in bacterial, blasticidin resistance in mammalian;
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: EvNO00877063
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CRISPLD2
Symbol Nomenclature : CRISPLD2
SYNONYM : CRISP11; LCRISP2
Designation : CRISP-11|LCCL domain containing cysteine-rich secretory protein 2|LCCL domain-containing cysteine-rich secretory protein 2|cysteine-rich secretory protein 11|cysteine-rich secretory protein LCCL domain-containing 2
Full Nomenclature : cysteine-rich secretory protein LCCL domain containing 2
GENEID : 83716
Locus Tag : UNQ2914/PRO1156/PRO9783
GenBank Accession : AM393137
HGNC : 25248
MIM : 612434
Vega : OTTHUMG00000137644
Target GenBank: AM393137

 

Reference Sequence Alignment

  

HsCD00438942        ATGAGCTGCGTCCTGGGTGGTGTCATCCCCTTGGGGCTGCTGTTCCTGGTCTGCGGATCC
XM_005256190.1      ATGAGCTGCGTCCTGGGTGGTGTCATCCCCTTGGGGCTGCTGTTCCTGGTCTGCGGATCC
NM_031476.3         ATGAGCTGCGTCCTGGGTGGTGTCATCCCCTTGGGGCTGCTGTTCCTGGTCTGCGGATCC
                    ************************************************************

HsCD00438942        CAAGGCTACCTCCTGCCCAACGTCACTCTCTTAGAGGAGCTGCTCAGCAAATACCAGCAC
XM_005256190.1      CAAGGCTACCTCCTGCCCAACGTCACTCTCTTAGAGGAGCTGCTCAGCAAATACCAGCAC
NM_031476.3         CAAGGCTACCTCCTGCCCAACGTCACTCTCTTAGAGGAGCTGCTCAGCAAATACCAGCAC
                    ************************************************************

HsCD00438942        AACGAGTCTCACTCCCGGGTCCGCAGAGCCATCCCCAGGGAGGACAAGGAGGAGATCCTC
XM_005256190.1      AACGAGTCTCACTCCCGGGTCCGCAGAGCCATCCCCAGGGAGGACAAGGAGGAGATCCTC
NM_031476.3         AACGAGTCTCACTCCCGGGTCCGCAGAGCCATCCCCAGGGAGGACAAGGAGGAGATCCTC
                    ************************************************************

HsCD00438942        ATGCTGCACAACAAGCTTCGGGGCCAGGTGCAGCCTCAGGCCTCCAACATGGAGTACATG
XM_005256190.1      ATGCTGCACAACAAGCTTCGGGGCCAGGTGCAGCCTCAGGCCTCCAACATGGAGTACATG
NM_031476.3         ATGCTGCACAACAAGCTTCGGGGCCAGGTGCAGCCTCAGGCCTCCAACATGGAGTACATG
                    ************************************************************

HsCD00438942        ACCTGGGATGACGAACTGGAGAAGTCTGCTGCAGCGTGGGCCAGTCAGTGCATCTGGGAG
XM_005256190.1      ACCTGGGATGACGAACTGGAGAAGTCTGCTGCAGCGTGGGCCAGTCAGTGCATCTGGGAG
NM_031476.3         ACCTGGGATGACGAACTGGAGAAGTCTGCTGCAGCGTGGGCCAGTCAGTGCATCTGGGAG
                    ************************************************************

HsCD00438942        CACGGGCCCACCGGTCTGCTGGTGTCCATCGGGCAGAACCTGGGCGCTCACTGGGGCAGG
XM_005256190.1      CACGGGCCCACCAGTCTGCTGGTGTCCATCGGGCAGAACCTGGGCGCTCACTGGGGCAGG
NM_031476.3         CACGGGCCCACCAGTCTGCTGGTGTCCATCGGGCAGAACCTGGGCGCTCACTGGGGCAGG
                    ************.***********************************************

HsCD00438942        TATCGCTCTCCGGGGTTCCATGTGCAGTCCTGGTATGACGAGGTGAAGGACTACACCTAC
XM_005256190.1      TATCGCTCTCCGGGGTTCCATGTGCAGTCCTGGTATGACGAGGTGAAGGACTACACCTAC
NM_031476.3         TATCGCTCTCCGGGGTTCCATGTGCAGTCCTGGTATGACGAGGTGAAGGACTACACCTAC
                    ************************************************************

HsCD00438942        CCCTACCCGAGCGAGTGCAACCCCTGGTGTCCAGAGAGGTGCTCGGGGCCTATGTGCACG
XM_005256190.1      CCCTACCCGAGCGAGTGCAACCCCTGGTGTCCAGAGAGGTGCTCGGGGCCCATGTGCACG
NM_031476.3         CCCTACCCGAGCGAGTGCAACCCCTGGTGTCCAGAGAGGTGCTCGGGGCCCATGTGCACG
                    ************************************************** *********

HsCD00438942        CACTACACACAGATAGTTTGGGCCACCACCAACAAGATCGGTTGTGCTGTGAACACCTGC
XM_005256190.1      CACTACACACAGATAGTTTGGGCCACCACCAACAAGATCGGTTGTGCTGTGAACACCTGC
NM_031476.3         CACTACACACAGATAGTTTGGGCCACCACCAACAAGATCGGTTGTGCTGTGAACACCTGC
                    ************************************************************

HsCD00438942        CGGAAGATGACTGTCTGGGGAGAAGTTTGGGAGAACGCGGTCTACTTTGTCTGCAATTAT
XM_005256190.1      CGGAAGATGACTGTCTGGGGAGAAGTTTGGGAGAACGCGGTCTACTTTGTCTGCAATTAT
NM_031476.3         CGGAAGATGACTGTCTGGGGAGAAGTTTGGGAGAACGCGGTCTACTTTGTCTGCAATTAT
                    ************************************************************

HsCD00438942        TCTCCAAAGGGGAACTGGATTGGAGAAGCCCCCTACAAGAATGGCCGGCCCTGCTCTGAG
XM_005256190.1      TCTCCAAAGGGGAACTGGATTGGAGAAGCCCCCTACAAGAATGGCCGGCCCTGCTCTGAG
NM_031476.3         TCTCCAAAGGGGAACTGGATTGGAGAAGCCCCCTACAAGAATGGCCGGCCCTGCTCTGAG
                    ************************************************************

HsCD00438942        TGCCCACCCAGCTATGGAGGCAGCTGCAGGAACAACTTGTGTTACCGAGAAGAAACCTAC
XM_005256190.1      TGCCCACCCAGCTATGGAGGCAGCTGCAGGAACAACTTGTGTTACCGAGAAGAAACCTAC
NM_031476.3         TGCCCACCCAGCTATGGAGGCAGCTGCAGGAACAACTTGTGTTACCGAGAAGAAACCTAC
                    ************************************************************

HsCD00438942        ACTCCAAAACCTGAAACGGACGAGATGAATGAGGTGGAAACGGCTCCCATTCCTGAAGAA
XM_005256190.1      ACTCCAAAACCTGAAACGGACGAGATGAATGAGGTGGAAACGGCTCCCATTCCTGAAGAA
NM_031476.3         ACTCCAAAACCTGAAACGGACGAGATGAATGAGGTGGAAACGGCTCCCATTCCTGAAGAA
                    ************************************************************

HsCD00438942        AACCATGTTTGGCTCCAACCGAGGGTGATGAGACCCACCAAGCCCAAGAAAACCTCTGCG
XM_005256190.1      AACCATGTTTGGCTCCAACCGAGGGTGATGAGACCCACCAAGCCCAAGAAAACCTCTGCG
NM_031476.3         AACCATGTTTGGCTCCAACCGAGGGTGATGAGACCCACCAAGCCCAAGAAAACCTCTGCG
                    ************************************************************

HsCD00438942        GTCAACTACATGACCCAAGTCGTCAGATGTGACACCAAGATGAAGGACAGGTGCAAAGGG
XM_005256190.1      GTCAACTACATGACCCAAGTCGTCAGATGTGACACCAAGATGAAGGACAGGTGCAAAGGG
NM_031476.3         GTCAACTACATGACCCAAGTCGTCAGATGTGACACCAAGATGAAGGACAGGTGCAAAGGG
                    ************************************************************

HsCD00438942        TCCACGTGTAACAGGTACCAGTGCCCAGCAGGCTGCCTGAACCACAAGGCGAAGATCTTT
XM_005256190.1      TCCACGTGTAACAGGTACCAGTGCCCAGCAGGCTGCCTGAACCACAAGGCGAAGATCTTT
NM_031476.3         TCCACGTGTAACAGGTACCAGTGCCCAGCAGGCTGCCTGAACCACAAGGCGAAGATCTTT
                    ************************************************************

HsCD00438942        GGAAGTCTGTTCTATGAAAGCTCGTCTAGCATATGCCGCGCCGCCATCCACTACGGGATC
XM_005256190.1      GGAACTCTGTTCTATGAAAGCTCGTCTAGCATATGCCGCGCCGCCATCCACTACGGGATC
NM_031476.3         GGAACTCTGTTCTATGAAAGCTCGTCTAGCATATGCCGCGCCGCCATCCACTACGGGATC
                    **** *******************************************************

HsCD00438942        CTGGATGACAAGGGAGGCCTGGTGGATATCACCAGGAACGGGAAGGTCCCCTTCTTCGTG
XM_005256190.1      CTGGATGACAAGGGAGGCCTGGTGGATATCACCAGGAACGGGAAGGTCCCCTTCTTCGTG
NM_031476.3         CTGGATGACAAGGGAGGCCTGGTGGATATCACCAGGAACGGGAAGGTCCCCTTCTTCGTG
                    ************************************************************

HsCD00438942        AAGTCTGAGAGACACGGCGTGCAGTCCCTCAGCAAATACAAACCTTCCAGCTCATTCATG
XM_005256190.1      AAGTCTGAGAGACACGGCGTGCAGTCCCTCAGCAAATACAAACCTTCCAGCTCATTCATG
NM_031476.3         AAGTCTGAGAGACACGGCGTGCAGTCCCTCAGCAAATACAAACCTTCCAGCTCATTCATG
                    ************************************************************

HsCD00438942        GTGTCAAAAGTGAAAGTGCAGGATTTGGACTGCTACACGACCGTTGCTCAGCTGTGCCCG
XM_005256190.1      GTGTCAAAAGTGAAAGTGCAGGATTTGGACTGCTACACGACCGTTGCTCAGCTGTGCCCG
NM_031476.3         GTGTCAAAAGTGAAAGTGCAGGATTTGGACTGCTACACGACCGTTGCTCAGCTGTGCCCG
                    ************************************************************

HsCD00438942        TTTGAAAAGCCAGCAACTCACTGCCCAAGAATCCATTGTCCGGCACACTGCAAAGACGAA
XM_005256190.1      TTTGAAAAGCCAGCAACTCACTGCCCAAGAATCCATTGTCCGGCACACTGCAAAGACGAA
NM_031476.3         TTTGAAAAGCCAGCAACTCACTGCCCAAGAATCCATTGTCCGGCACACTGCAAAGACGAA
                    ************************************************************

HsCD00438942        CCTTCCTACTGGGCTCCGGTGTTTGGAACCAACATCTATGCAGATGTGAGTAGGATGCAT
XM_005256190.1      CCTTCCTACTGGGCTCCGGTGTTTGGAACCAACATCTATGCAGATACCTCAAGCATCTGC
NM_031476.3         CCTTCCTACTGGGCTCCGGTGTTTGGAACCAACATCTATGCAGATACCTCAAGCATCTGC
                    *********************************************.  : :** **  . 

HsCD00438942        TTTCAACAACTATCTCGCAAAGCCTGT---------------------------------
XM_005256190.1      AAGACAGCCGTGCACGCGGGAGTCATCAGCAACGAGAGTGGGGGTGACGTGGACGTGATG
NM_031476.3         AAGACAGCCGTGCACGCGGGAGTCATCAGCAACGAGAGTGGGGGTGACGTGGACGTGATG
                    :: ..* .. *. .    ..** *:                                   

HsCD00438942        ------------------------------------------------------------
XM_005256190.1      CCCGTGGATAAAAAGAAGACCTACGTGGGCTCGCTCAGGAATGGAGTTCAGTCTGAAAGC
NM_031476.3         CCCGTGGATAAAAAGAAGACCTACGTGGGCTCGCTCAGGAATGGAGTTCAGTCTGAAAGC
                                                                                

HsCD00438942        ------------------------------------------------------
XM_005256190.1      CTGGGGACTCCTCGGGATGGAAAGGCCTTCCGGATCTTTGCTGTCAGGCAGTGA
NM_031476.3         CTGGGGACTCCTCGGGATGGAAAGGCCTTCCGGATCTTTGCTGTCAGGCAGTGA
                                                                          

Clone

Original Clone ID : ccsbBroad304_12752

Organism


TAX_ID : 9606
Species Specific ID: 83716

Genome

Chromosome : 16
Map Location : 16q24.1
Ensembl : ENSG00000103196

Reagents

Labome : CRISPLD2-antibody