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NET1 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  NET1
Gene Name:  neuroepithelial cell transforming 1
Sequence             
Original Clone ID: ccsbBroad304_02379
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00438981

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1789nts         Open reading frame : 1 to 1789
Reference Sequence Alignment

ATGGAGCCCGAGCTGGCGGCTCAGAAGCAGCCTCGACCGCGGAGGCGAAGCCGCCGGGCC
TCTGGGCTCAGCACGGAGGGAGCGACGGGGCCTTCGGCCGACACCTCCGGGTCGGAGCTG
GACGGGAGATGTTCCCTTCGGAGAGGCAGCTCCTTCACATTCTTAACACCTGGCCCCAAC
TGGGACTTCACTTTGAAAAGAAAACGCAGAGAGAAAGATGATGATGTTGTAAGCCTTAGC
AGCCTTGATCTGAAGGAGCCAAGCAATAAAAGAGTTCGACCTCTGGCTCGTGTCACGTCC
TTGGCAAATTTAATCTCTCCTGTAAGAAATGGAGCTGTCAGACGTTTTGGTCAAACAATA
CAGTCATTTACCCTTCGTGGTGACCACAGATCCCCAGCCTCTGCCCAGAAGTTTTCTAGC
AGGTCAACAGTCCCAACACCCGCCAAGAGAAGGAGCAGTGCACTGTGGTCAGAGATGCTG
GACATCACCATGAAGGAGTCTCTCACCACCAGGGAGATCAGACGGCAGGAGGCAATATAT
GAAATGTCCCGAGGTGAACAGGATTTAATTGAGGATCTCAAACTTGCAAGAAAGGCCTAC
CATGACCCCATGTTAAAGTTGTCCATCATGTCAGAAGAGGAACTCACACATATATTTGGT
GATCTGGACTCTTACATACCTCTGCATGAAGATTTGTTGACAAGAATAGGAGAAGCAACC
AAGCCTGATGGAACAGTGGAGCAGATTGGTCACATTCTCGTGAGCTGGTTACCGCGCTTG
AATGCCTACAGAGGTTACTGTAGTAACCAGCTGGCAGCCAAAGCTCTTCTTGATCAAAAG
AAACAGGATCCAAGAGTCCAAGACTTCCTCCAGCGATGTCTCGAGTCTCCCTTCAGTCGA
AAACTAGATCTTTGGAGTTTCCTAGATATCCCTCGAAGTCGCCTAGTCAAATACCCTTTA
CTGTTAAAAGAAATTCTTAAACACACTCCAAAAGAGCACCCTGATGTTCAGCTTCTGGAG
GATGCTATATTGATAATACAGGGAGTCCTCTCTGATATCAACTTGAAGAAAGGTGAATCC
GAGTGCCAGTATTACATCGACAAGCTGGAGTACCTGGATGAAAAGCAGAGGGACCCCAGA
ATCGAAGCGAGCAAAGTGCTGCTGTGCCATGGGGAGCTGCGGAGCAAGAGTGGACATAAA
CTTTACATTTTCCTGTTTCAAGACATCTTGGTTCTGACTCGGCCCGTCACACGGAACGAA
CGGCACTCTTACCAGGTTTACCGGCAGCCAATCCCAGTCCAAGAGCTAGTCCTAGAAGAC
CTGCAGGATGGAGATGTGAGAATGGGAGGCTCCTTTCGAGGAGCTTTCAGTAACTCAGAG
AAAGCTAAAAATATCTTTAGAATTCGCTTCCATGACCCCTCTCCAGCCCAGTCTCACACT
CTGCAAGCCAATGACGTGTTCCACAAGCAGCAGTGGTTCAACTGTATTCGAGCGGCCATT
GCCCCCTTCCAGTCGGCAGGCAGTCCACCTGAGCTGCAGGGCCTGCCGGAGCTGCACGAA
GAGTGTGAGGGGAACCACCCCTCTGCGAGGAAACTCACAGCCCAGAGGAGGGCATCCACA
GTTTCCAGTGTTACTCAGGTAGAAGTTGATGAAAACGCTTACAGATGTGGCTCTGGCATG
CAGATGGCAGAGGACAGCAAGAGCTTAAAGACACACCAGACACAGCCCGGCATCCGAAGA
GCGAGGGACAAAGCCCTTTCTGGTGGCAAACGGAAAGAGACTTTGGTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : NET1
Symbol Nomenclature : NET1
SYNONYM : ARHGEF8; NET1A
Designation : Rho guanine nucleotide exchange factor (GEF) 8|guanine nucleotide regulatory protein (oncogene)|neuroepithelial cell-transforming gene 1 protein|neuroepithelioma transforming gene 1|p65 Net1 proto-oncogene protein|small GTP-binding protein regulator
Full Nomenclature : neuroepithelial cell transforming 1
GENEID : 10276
Locus Tag : RP11-116G8.1
HGNC : 14592
MIM : 606450
Vega : OTTHUMG00000017596
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00438981        ATGGAGCCCGAGCTGGCGGCTCAGAAGCAGCCTCGACCGCGGAGGCGAAGCCGCCGGGCC
NM_001047160.2      ATGGAGCCCGAGCTGGCGGCTCAGAAGCAGCCTCGACCGCGGAGGCGAAGCCGCCGGGCC
NM_005863.4         ------------------------------------------------------------
                                                                                

HsCD00438981        TCTGGGCTCAGCACGGAGGGAGCGACGGGGCCTTCGGCCGACACCTCCGGGTCGGAGCTG
NM_001047160.2      TCTGGGCTCAGCACGGAGGGAGCGACGGGGCCTTCGGCCGACACCTCCGGGTCGGAGCTG
NM_005863.4         ------------------------------------------------------------
                                                                                

HsCD00438981        GACGGGAGATGTTCCCTTCGGAGAGGCAGCTCCTTCACATTCTTAACACCTGGCCCCAAC
NM_001047160.2      GACGGGAGATGTTCCCTTCGGAGAGGCAGCTCCTTCACATTCTTAACACCTGGCCCCAAC
NM_005863.4         ------------------------------------------ATGGTGGCACATGATGAG
                                                              :*.. . *: .  . .* 

HsCD00438981        TGGGACTTCACTTTGAAAAGAAAACGCAGAGAGAAAGATGATGATGTTGTAAGCCTTAGC
NM_001047160.2      TGGGACTTCACTTTGAAAAGAAAACGCAGAGAGAAAGATGATGATGTTGTAAGCCTTAGC
NM_005863.4         ACTGGAGGTCTCCTACCTATTAAAAGGACCATACGAGTCCTAGATGTCAATAACCAGTCC
                    :  *..   .   *...:* :***.* * ..:...**:  ::***** .::*.**: : *

HsCD00438981        AGCCTTGATCTGAAGGAGCCAAGCAATAAAAGAGTTCGACCTCTGGCTCGTGTCACGTCC
NM_001047160.2      AGCCTTGATCTGAAGGAGCCAAGCAATAAAAGAGTTCGACCTCTGGCTCGTGTCACGTCC
NM_005863.4         TTCAGAGAACAAGAGGAGCCAAGCAATAAAAGAGTTCGACCTCTGGCTCGTGTCACGTCC
                    : *. :**:*:..***********************************************

HsCD00438981        TTGGCAAATTTAATCTCTCCTGTAAGAAATGGAGCTGTCAGACGTTTTGGTCAAACAATA
NM_001047160.2      TTGGCAAATTTAATCTCTCCTGTAAGAAATGGAGCTGTCAGACGTTTTGGTCAAACAATA
NM_005863.4         TTGGCAAATTTAATCTCTCCTGTAAGAAATGGAGCTGTCAGACGTTTTGGTCAAACAATA
                    ************************************************************

HsCD00438981        CAGTCATTTACCCTTCGTGGTGACCACAGATCCCCAGCCTCTGCCCAGAAGTTTTCTAGC
NM_001047160.2      CAGTCATTTACCCTTCGTGGTGACCACAGATCCCCAGCCTCTGCCCAGAAGTTTTCTAGC
NM_005863.4         CAGTCATTTACCCTTCGTGGTGACCACAGATCCCCAGCCTCTGCCCAGAAGTTTTCTAGC
                    ************************************************************

HsCD00438981        AGGTCAACAGTCCCAACACCCGCCAAGAGAAGGAGCAGTGCACTGTGGTCAGAGATGCTG
NM_001047160.2      AGGTCAACAGTCCCAACACCCGCCAAGAGAAGGAGCAGTGCACTGTGGTCAGAGATGCTG
NM_005863.4         AGGTCAACAGTCCCAACACCCGCCAAGAGAAGGAGCAGTGCACTGTGGTCAGAGATGCTG
                    ************************************************************

HsCD00438981        GACATCACCATGAAGGAGTCTCTCACCACCAGGGAGATCAGACGGCAGGAGGCAATATAT
NM_001047160.2      GACATCACCATGAAGGAGTCTCTCACCACCAGGGAGATCAGACGGCAGGAGGCAATATAT
NM_005863.4         GACATCACCATGAAGGAGTCTCTCACCACCAGGGAGATCAGACGGCAGGAGGCAATATAT
                    ************************************************************

HsCD00438981        GAAATGTCCCGAGGTGAACAGGATTTAATTGAGGATCTCAAACTTGCAAGAAAGGCCTAC
NM_001047160.2      GAAATGTCCCGAGGTGAACAGGATTTAATTGAGGATCTCAAACTTGCAAGAAAGGCCTAC
NM_005863.4         GAAATGTCCCGAGGTGAACAGGATTTAATTGAGGATCTCAAACTTGCAAGAAAGGCCTAC
                    ************************************************************

HsCD00438981        CATGACCCCATGTTAAAGTTGTCCATCATGTCAGAAGAGGAACTCACACATATATTTGGT
NM_001047160.2      CATGACCCCATGTTAAAGTTGTCCATCATGTCAGAAGAGGAACTCACACATATATTTGGT
NM_005863.4         CATGACCCCATGTTAAAGTTGTCCATCATGTCAGAAGAGGAACTCACACATATATTTGGT
                    ************************************************************

HsCD00438981        GATCTGGACTCTTACATACCTCTGCATGAAGATTTGTTGACAAGAATAGGAGAAGCAACC
NM_001047160.2      GATCTGGACTCTTACATACCTCTGCATGAAGATTTGTTGACAAGAATAGGAGAAGCAACC
NM_005863.4         GATCTGGACTCTTACATACCTCTGCATGAAGATTTGTTGACAAGAATAGGAGAAGCAACC
                    ************************************************************

HsCD00438981        AAGCCTGATGGAACAGTGGAGCAGATTGGTCACATTCTCGTGAGCTGGTTACCGCGCTTG
NM_001047160.2      AAGCCTGATGGAACAGTGGAGCAGATTGGTCACATTCTCGTGAGCTGGTTACCGCGCTTG
NM_005863.4         AAGCCTGATGGAACAGTGGAGCAGATTGGTCACATTCTCGTGAGCTGGTTACCGCGCTTG
                    ************************************************************

HsCD00438981        AATGCCTACAGAGGTTACTGTAGTAACCAGCTGGCAGCCAAAGCTCTTCTTGATCAAAAG
NM_001047160.2      AATGCCTACAGAGGTTACTGTAGTAACCAGCTGGCAGCCAAAGCTCTTCTTGATCAAAAG
NM_005863.4         AATGCCTACAGAGGTTACTGTAGTAACCAGCTGGCAGCCAAAGCTCTTCTTGATCAAAAG
                    ************************************************************

HsCD00438981        AAACAGGATCCAAGAGTCCAAGACTTCCTCCAGCGATGTCTCGAGTCTCCCTTCAGTCGA
NM_001047160.2      AAACAGGATCCAAGAGTCCAAGACTTCCTCCAGCGATGTCTCGAGTCTCCCTTCAGTCGA
NM_005863.4         AAACAGGATCCAAGAGTCCAAGACTTCCTCCAGCGATGTCTCGAGTCTCCCTTCAGTCGA
                    ************************************************************

HsCD00438981        AAACTAGATCTTTGGAGTTTCCTAGATATCCCTCGAAGTCGCCTAGTCAAATACCCTTTA
NM_001047160.2      AAACTAGATCTTTGGAGTTTCCTAGATATCCCTCGAAGTCGCCTAGTCAAATACCCTTTA
NM_005863.4         AAACTAGATCTTTGGAGTTTCCTAGATATCCCTCGAAGTCGCCTAGTCAAATACCCTTTA
                    ************************************************************

HsCD00438981        CTGTTAAAAGAAATTCTTAAACACACTCCAAAAGAGCACCCTGATGTTCAGCTTCTGGAG
NM_001047160.2      CTGTTAAAAGAAATTCTTAAACACACTCCAAAAGAGCACCCTGATGTTCAGCTTCTGGAG
NM_005863.4         CTGTTAAAAGAAATTCTTAAACACACTCCAAAAGAGCACCCTGATGTTCAGCTTCTGGAG
                    ************************************************************

HsCD00438981        GATGCTATATTGATAATACAGGGAGTCCTCTCTGATATCAACTTGAAGAAAGGTGAATCC
NM_001047160.2      GATGCTATATTGATAATACAGGGAGTCCTCTCTGATATCAACTTGAAGAAAGGTGAATCC
NM_005863.4         GATGCTATATTGATAATACAGGGAGTCCTCTCTGATATCAACTTGAAGAAAGGTGAATCC
                    ************************************************************

HsCD00438981        GAGTGCCAGTATTACATCGACAAGCTGGAGTACCTGGATGAAAAGCAGAGGGACCCCAGA
NM_001047160.2      GAGTGCCAGTATTACATCGACAAGCTGGAGTACCTGGATGAAAAGCAGAGGGACCCCAGA
NM_005863.4         GAGTGCCAGTATTACATCGACAAGCTGGAGTACCTGGATGAAAAGCAGAGGGACCCCAGA
                    ************************************************************

HsCD00438981        ATCGAAGCGAGCAAAGTGCTGCTGTGCCATGGGGAGCTGCGGAGCAAGAGTGGACATAAA
NM_001047160.2      ATCGAAGCGAGCAAAGTGCTGCTGTGCCATGGGGAGCTGCGGAGCAAGAGTGGACATAAA
NM_005863.4         ATCGAAGCGAGCAAAGTGCTGCTGTGCCATGGGGAGCTGCGGAGCAAGAGTGGACATAAA
                    ************************************************************

HsCD00438981        CTTTACATTTTCCTGTTTCAAGACATCTTGGTTCTGACTCGGCCCGTCACACGGAACGAA
NM_001047160.2      CTTTACATTTTCCTGTTTCAAGACATCTTGGTTCTGACTCGGCCCGTCACACGGAACGAA
NM_005863.4         CTTTACATTTTCCTGTTTCAAGACATCTTGGTTCTGACTCGGCCCGTCACACGGAACGAA
                    ************************************************************

HsCD00438981        CGGCACTCTTACCAGGTTTACCGGCAGCCAATCCCAGTCCAAGAGCTAGTCCTAGAAGAC
NM_001047160.2      CGGCACTCTTACCAGGTTTACCGGCAGCCAATCCCAGTCCAAGAGCTAGTCCTAGAAGAC
NM_005863.4         CGGCACTCTTACCAGGTTTACCGGCAGCCAATCCCAGTCCAAGAGCTAGTCCTAGAAGAC
                    ************************************************************

HsCD00438981        CTGCAGGATGGAGATGTGAGAATGGGAGGCTCCTTTCGAGGAGCTTTCAGTAACTCAGAG
NM_001047160.2      CTGCAGGATGGAGATGTGAGAATGGGAGGCTCCTTTCGAGGAGCTTTCAGTAACTCAGAG
NM_005863.4         CTGCAGGATGGAGATGTGAGAATGGGAGGCTCCTTTCGAGGAGCTTTCAGTAACTCAGAG
                    ************************************************************

HsCD00438981        AAAGCTAAAAATATCTTTAGAATTCGCTTCCATGACCCCTCTCCAGCCCAGTCTCACACT
NM_001047160.2      AAAGCTAAAAATATCTTTAGAATTCGCTTCCATGACCCCTCTCCAGCCCAGTCTCACACT
NM_005863.4         AAAGCTAAAAATATCTTTAGAATTCGCTTCCATGACCCCTCTCCAGCCCAGTCTCACACT
                    ************************************************************

HsCD00438981        CTGCAAGCCAATGACGTGTTCCACAAGCAGCAGTGGTTCAACTGTATTCGAGCGGCCATT
NM_001047160.2      CTGCAAGCCAATGACGTGTTCCACAAGCAGCAGTGGTTCAACTGTATTCGAGCGGCCATT
NM_005863.4         CTGCAAGCCAATGACGTGTTCCACAAGCAGCAGTGGTTCAACTGTATTCGAGCGGCCATT
                    ************************************************************

HsCD00438981        GCCCCCTTCCAGTCGGCAGGCAGTCCACCTGAGCTGCAGGGCCTGCCGGAGCTGCACGAA
NM_001047160.2      GCCCCCTTCCAGTCGGCAGGCAGTCCACCTGAGCTGCAGGGCCTGCCGGAGCTGCACGAA
NM_005863.4         GCCCCCTTCCAGTCGGCAGGCAGTCCACCTGAGCTGCAGGGCCTGCCGGAGCTGCACGAA
                    ************************************************************

HsCD00438981        GAGTGTGAGGGGAACCACCCCTCTGCGAGGAAACTCACAGCCCAGAGGAGGGCATCCACA
NM_001047160.2      GAGTGTGAGGGGAACCACCCCTCTGCGAGGAAACTCACAGCCCAGAGGAGGGCATCCACA
NM_005863.4         GAGTGTGAGGGGAACCACCCCTCTGCGAGGAAACTCACAGCCCAGAGGAGGGCATCCACA
                    ************************************************************

HsCD00438981        GTTTCCAGTGTTACTCAGGTAGAAGTTGATGAAAACGCTTACAGATGTGGCTCTGGCATG
NM_001047160.2      GTTTCCAGTGTTACTCAGGTAGAAGTTGATGAAAACGCTTACAGATGTGGCTCTGGCATG
NM_005863.4         GTTTCCAGTGTTACTCAGGTAGAAGTTGATGAAAACGCTTACAGATGTGGCTCTGGCATG
                    ************************************************************

HsCD00438981        CAGATGGCAGAGGACAGCAAGAGCTTAAAGACACACCAGACACAGCCCGGCATCCGAAGA
NM_001047160.2      CAGATGGCAGAGGACAGCAAGAGCTTAAAGACACACCAGACACAGCCCGGCATCCGAAGA
NM_005863.4         CAGATGGCAGAGGACAGCAAGAGCTTAAAGACACACCAGACACAGCCCGGCATCCGAAGA
                    ************************************************************

HsCD00438981        GCGAGGGACAAAGCCCTTTCTGGTGGCAAACGGAAAGAGACTTTGGTG---
NM_001047160.2      GCGAGGGACAAAGCCCTTTCTGGTGGCAAACGGAAAGAGACTTTGGTGTAG
NM_005863.4         GCGAGGGACAAAGCCCTTTCTGGTGGCAAACGGAAAGAGACTTTGGTGTAG
                    ************************************************   

Pathways

NCI : Regulation of RhoA activity
Reactome : Cell death signalling via NRAGE, NRIF and NADE
Reactome : G alpha (12/13) signalling events
Reactome : GPCR downstream signaling
Reactome : NRAGE signals death through JNK
Reactome : Rho GTPase cycle
Reactome : Signal Transduction
Reactome : Signaling by GPCR
Reactome : Signaling by Rho GTPases
Reactome : Signalling by NGF
Reactome : p75 NTR receptor-mediated signalling

Clone

Original Clone ID : ccsbBroad304_02379

Organism


TAX_ID : 9606
Species Specific ID: 10276

Genome

Chromosome : 10
Map Location : 10p15
Ensembl : ENSG00000173848

Reagents

Labome : NET1-antibody