DNASU Plasmid Repository • 480.965.5697 | Email

PVRL2 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  PVRL2
Gene Name:  poliovirus receptor-related 2 (herpesvirus entry mediator B)
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH180345.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00043912

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1440nts         Open reading frame : 1 to 1440
Reference Sequence Alignment

ATGGCCCGGGCCGCTGCCCTCCTGCCGTCGAGATCGCCGCCGACGCCGCTGCTGTGGCCG
CTGCTGCTGCTGCTGCTCCTGGAAACCGGAGCCCAGGATGTGCGAGTTCAAGTGCTACCC
GAGGTGCGAGGCCAGCTCGGGGGCACCGTGGAGCTGCCGTGCCACCTGCTGCCACCTGTT
CCTGGACTGTACATCTCCCTGGTGACCTGGCAGCGCCCAGATGCACCTGCGAACCACCAG
AATGTGGCCGCCTTCCACCCTAAGATGGGTCCCAGCTTCCCCAGCCCGAAGCCTGGCAGC
GAGCGGCTGTCCTTCGTCTCTGCCAAGCAGAGCACTGGGCAAGACACAGAGGCAGAGCTC
CAGGACGCCACGCTGGCCCTCCACGGGCTCACGGTGGAGGACGAGGGCAACTACACTTGC
GAGTTTGCCACCTTCCCCAAGGGGTCCGTCCGAGGGATGACCTGGCTCAGAGTCATAGCC
AAGCCCAAGAACCAAGCTGAGGCCCAGAAGGTCACGTTCAGCCAGGACCCTACGACAGTG
GCCCTCTGCATCTCCAAAGAGGGCCGCCCACCTGCCCGGATCTCCTGGCTCTCATCCCTG
GACTGGGAAGCCAAAGAGACTCAGGTGTCAGGGACCCTGGCCGGAACTGTCACTGTCACC
AGCCGCTTCACCTTGGTGCCCTCGGGCCGAGCAGATGGTGTCACGGTCACCTGCAAAGTG
GAGCATGAGAGCTTCGAGGAACCAGCCCTGATACCTGTGACCCTCTCTGTACGCTACCCT
CCTGAAGTGTCCATCTCCGGCTATGATGACAACTGGTACCTCGGCCGTACTGATGCCACC
CTGAGCTGTGACGTCCGCAGCAACCCAGAGCCCACGGGCTATGACTGGAGCACGACCTCA
GGCACCTTCCCGACCTCCGCAGTGGCCCAGGGCTCCCAGCTGGTCATCCACGCAGTGGAC
AGTCTGTTCAATACCACCTTCGTCTGCACAGTCACCAATGCCGTGGGCATGGGCCGCGCT
GAGCAGGTCATCTTTGTCCGAGAAACCCCCAGGGCCTCGCCCCGAGATGTGGGCCCGCTG
GTGTGGGGGGCCGTGGGGGGGACACTGCTGGTGCTGCTGCTTCTGGCTGGGGGGTCCTTG
GCCTTCATCCTGCTGAGGGTGAGGAGGAGGAGGAAGAGCCCTGGAGGAGCAGGAGGAGGA
GCCAGTGGCGACGGGGGATTCTACGATCCGAAAGCTCAGGTGTTGGGAAATGGGGACCCC
GTCTTCTGGACACCAGTAGTCCCTGGTCCCATGGAACCAGATGGCAAGGATGAGGAGGAG
GAGGAGGAGGAAGAGAAGGCAGAGAAAGGCCTCATGTTGCCTCCACCCCCAGCACTCGAG
GATGACATGGAGTCCCAGCTGGACGGCTCCCTCATCTCACGGCGGGCAGTTTATGTGTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1638
Start on reference sequence 199
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : PVRL2
Symbol Nomenclature : PVRL2
SYNONYM : CD112; HVEB; PRR2; PVRR2
Designation : herpesvirus entry protein B|nectin-2|poliovirus receptor-like 2|poliovirus receptor-related protein 2
Full Nomenclature : poliovirus receptor-related 2 (herpesvirus entry mediator B)
GENEID : 5819
HGNC : 9707
MIM : 600798
Vega : OTTHUMG00000180839
Target GenBank: BC003091

 

Reference Sequence Alignment

  

HsCD00043912      ATGGCCCGGGCCGCTGCCCTCCTGCCGTCGAGATCGCCGCCGACGCCGCTGCTGTGGCCG
NM_002856.2       ATGGCCCGGGCCGCTGCCCTCCTGCCGTCGAGATCGCCGCCGACGCCGCTGCTGTGGCCG
                  ************************************************************

HsCD00043912      CTGCTGCTGCTGCTGCTCCTGGAAACCGGAGCCCAGGATGTGCGAGTTCAAGTGCTACCC
NM_002856.2       CTGCTGCTGCTGCTGCTCCTGGAAACCGGAGCCCAGGATGTGCGAGTTCAAGTGCTACCC
                  ************************************************************

HsCD00043912      GAGGTGCGAGGCCAGCTCGGGGGCACCGTGGAGCTGCCGTGCCACCTGCTGCCACCTGTT
NM_002856.2       GAGGTGCGAGGCCAGCTCGGGGGCACCGTGGAGCTGCCGTGCCACCTGCTGCCACCTGTT
                  ************************************************************

HsCD00043912      CCTGGACTGTACATCTCCCTGGTGACCTGGCAGCGCCCAGATGCACCTGCGAACCACCAG
NM_002856.2       CCTGGACTGTACATCTCCCTGGTGACCTGGCAGCGCCCAGATGCACCTGCGAACCACCAG
                  ************************************************************

HsCD00043912      AATGTGGCCGCCTTCCACCCTAAGATGGGTCCCAGCTTCCCCAGCCCGAAGCCTGGCAGC
NM_002856.2       AATGTGGCCGCCTTCCACCCTAAGATGGGTCCCAGCTTCCCCAGCCCGAAGCCTGGCAGC
                  ************************************************************

HsCD00043912      GAGCGGCTGTCCTTCGTCTCTGCCAAGCAGAGCACTGGGCAAGACACAGAGGCAGAGCTC
NM_002856.2       GAGCGGCTGTCCTTCGTCTCTGCCAAGCAGAGCACTGGGCAAGACACAGAGGCAGAGCTC
                  ************************************************************

HsCD00043912      CAGGACGCCACGCTGGCCCTCCACGGGCTCACGGTGGAGGACGAGGGCAACTACACTTGC
NM_002856.2       CAGGACGCCACGCTGGCCCTCCACGGGCTCACGGTGGAGGACGAGGGCAACTACACTTGC
                  ************************************************************

HsCD00043912      GAGTTTGCCACCTTCCCCAAGGGGTCCGTCCGAGGGATGACCTGGCTCAGAGTCATAGCC
NM_002856.2       GAGTTTGCCACCTTCCCCAAGGGGTCCGTCCGAGGGATGACCTGGCTCAGAGTCATAGCC
                  ************************************************************

HsCD00043912      AAGCCCAAGAACCAAGCTGAGGCCCAGAAGGTCACGTTCAGCCAGGACCCTACGACAGTG
NM_002856.2       AAGCCCAAGAACCAAGCTGAGGCCCAGAAGGTCACGTTCAGCCAGGACCCTACGACAGTG
                  ************************************************************

HsCD00043912      GCCCTCTGCATCTCCAAAGAGGGCCGCCCACCTGCCCGGATCTCCTGGCTCTCATCCCTG
NM_002856.2       GCCCTCTGCATCTCCAAAGAGGGCCGCCCACCTGCCCGGATCTCCTGGCTCTCATCCCTG
                  ************************************************************

HsCD00043912      GACTGGGAAGCCAAAGAGACTCAGGTGTCAGGGACCCTGGCCGGAACTGTCACTGTCACC
NM_002856.2       GACTGGGAAGCCAAAGAGACTCAGGTGTCAGGGACCCTGGCCGGAACTGTCACTGTCACC
                  ************************************************************

HsCD00043912      AGCCGCTTCACCTTGGTGCCCTCGGGCCGAGCAGATGGTGTCACGGTCACCTGCAAAGTG
NM_002856.2       AGCCGCTTCACCTTGGTGCCCTCGGGCCGAGCAGATGGTGTCACGGTCACCTGCAAAGTG
                  ************************************************************

HsCD00043912      GAGCATGAGAGCTTCGAGGAACCAGCCCTGATACCTGTGACCCTCTCTGTACGCTACCCT
NM_002856.2       GAGCATGAGAGCTTCGAGGAACCAGCCCTGATACCTGTGACCCTCTCTGTACGCTACCCT
                  ************************************************************

HsCD00043912      CCTGAAGTGTCCATCTCCGGCTATGATGACAACTGGTACCTCGGCCGTACTGATGCCACC
NM_002856.2       CCTGAAGTGTCCATCTCCGGCTATGATGACAACTGGTACCTCGGCCGTACTGATGCCACC
                  ************************************************************

HsCD00043912      CTGAGCTGTGACGTCCGCAGCAACCCAGAGCCCACGGGCTATGACTGGAGCACGACCTCA
NM_002856.2       CTGAGCTGTGACGTCCGCAGCAACCCAGAGCCCACGGGCTATGACTGGAGCACGACCTCA
                  ************************************************************

HsCD00043912      GGCACCTTCCCGACCTCCGCAGTGGCCCAGGGCTCCCAGCTGGTCATCCACGCAGTGGAC
NM_002856.2       GGCACCTTCCCGACCTCCGCAGTGGCCCAGGGCTCCCAGCTGGTCATCCACGCAGTGGAC
                  ************************************************************

HsCD00043912      AGTCTGTTCAATACCACCTTCGTCTGCACAGTCACCAATGCCGTGGGCATGGGCCGCGCT
NM_002856.2       AGTCTGTTCAATACCACCTTCGTCTGCACAGTCACCAATGCCGTGGGCATGGGCCGCGCT
                  ************************************************************

HsCD00043912      GAGCAGGTCATCTTTGTCCGAGAAACCCCCAGGGCCTCGCCCCGAGATGTGGGCCCGCTG
NM_002856.2       GAGCAGGTCATCTTTGTCCGAGAAACCCCCAGGGCCTCGCCCCGAGATGTGGGCCCGCTG
                  ************************************************************

HsCD00043912      GTGTGGGGGGCCGTGGGGGGGACACTGCTGGTGCTGCTGCTTCTGGCTGGGGGGTCCTTG
NM_002856.2       GTGTGGGGGGCCGTGGGGGGGACACTGCTGGTGCTGCTGCTTCTGGCTGGGGGGTCCTTG
                  ************************************************************

HsCD00043912      GCCTTCATCCTGCTGAGGGTGAGGAGGAGGAGGAAGAGCCCTGGAGGAGCAGGAGGAGGA
NM_002856.2       GCCTTCATCCTGCTGAGGGTGAGGAGGAGGAGGAAGAGCCCTGGAGGAGCAGGAGGAGGA
                  ************************************************************

HsCD00043912      GCCAGTGGCGACGGGGGATTCTACGATCCGAAAGCTCAGGTGTTGGGAAATGGGGACCCC
NM_002856.2       GCCAGTGGCGACGGGGGATTCTACGATCCGAAAGCTCAGGTGTTGGGAAATGGGGACCCC
                  ************************************************************

HsCD00043912      GTCTTCTGGACACCAGTAGTCCCTGGTCCCATGGAACCAGATGGCAAGGATGAGGAGGAG
NM_002856.2       GTCTTCTGGACACCAGTAGTCCCTGGTCCCATGGAACCAGATGGCAAGGATGAGGAGGAG
                  ************************************************************

HsCD00043912      GAGGAGGAGGAAGAGAAGGCAGAGAAAGGCCTCATGTTGCCTCCACCCCCAGCACTCGAG
NM_002856.2       GAGGAGGAGGAAGAGAAGGCAGAGAAAGGCCTCATGTTGCCTCCACCCCCAGCACTCGAG
                  ************************************************************

HsCD00043912      GATGACATGGAGTCCCAGCTGGACGGCTCCCTCATCTCACGGCGGGCAGTTTATGTGTAG
NM_002856.2       GATGACATGGAGTCCCAGCTGGACGGCTCCCTCATCTCACGGCGGGCAGTTTATGTGTGA
                  **********************************************************..

Pathways

NCI : Nectin adhesion pathway
NCI : Stabilization and expansion of the E-cadherin adherens junction
Reactome : Adaptive Immune System
Reactome : Adherens junctions interactions
Reactome : Cell junction organization
Reactome : Cell-Cell communication
Reactome : Cell-cell junction organization
Reactome : Immune System
Reactome : Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell
Reactome : Nectin/Necl trans heterodimerization
WikiPathway : Monoamine Transport

Protein

SMART domain : IG : Immunoglobulin
SMART domain : IGc2 : Immunoglobulin C-2 Type
SMART domain : IGv : Immunoglobulin V-Type
UniProt : Q92692
HPRD : 02879

Clone

Cloning Information : 180345
HIP Master Clone ID : 155446
Original Clone ID : FLH180345.01X

Organism


TAX_ID : 9606
Species Specific ID: 5819

Genome

Chromosome : 19
Map Location : 19q13.2
Ensembl : ENSG00000130202

Reagents

Labome : PVRL2-antibody