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LGALS9C (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  LGALS9C
Gene Name:  lectin, galactoside-binding, soluble, 9C
Sequence             
Original Clone ID: ccsbBroad304_10195
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00439967

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1069nts         Open reading frame : 1 to 1069
Reference Sequence Alignment

ATGGCCTTCAGCGGTTGCCAGGCTCCCTATCTGAGCCCAGCCGTCCCCTTTTCTGGGACT
ATCCAAGGGGGTCTCCAGGACGGATTTCAGATCACTGTCAATGGGGCCGTTCTCAGCTGC
AGTGGAACCAGGTTTGCTGTGGACTTTCAGACGGGCTTCAGTGGAAACGACATTGCCTTC
CACTTCAACCCTCGGTTTGAAGACGGAGGGTATGTGGTGTGCAACACGAGGCAGAAAGGA
ACATGGGGGCCCGAGGAGAGGAAGATGCACATGCCCTTCCAGAAGGGGATGCCCTTTGAC
CTCTGCTTCCTGGTGCAGAGCTCAGATTTCAAGGTGATGGTGAACGGGAGCCTCTTCGTG
CAGTACTTCCACCGCGTGCCCTTCCACCGTGTGGACACCATCTCCGTCAATGGCTCTGTG
CAGCTGTCCTACATCAGCTTCCAGAATCCCCGCGCAGTCCCCGTTCAGCCTGCCTTCTCC
ACGGTGCCGTTCTCCCAGCCTGTCTGTTTCCCACCCAGGCCCAGGGGGCGCAGACAAAAA
CCTCCCATCGTGCGGCCTGCCAACCCAGCTCCCATTACCCAGACAGTCATCCACACGGTG
CAGAGTGCCTCTGGACAGATGTTCTCTCAGACTCCCGCCATCCCACCTATGATGTACCCC
CACCCTGCCTATCCGATGCCTTTCATCACCACCATTCCGGGAGGGCTGTACCCATCCAAG
TCCATCATCCTGTCAGGCACTGTCCTGCCCAGTGCTCAGAGGTTCCACATCAACCTGTGC
TCTGGGAGCCACATCGCCTTCCACATGAACCCCCGTTTTGATGAGAATGCTGTGGTCCGT
AACACCCAGATCAACAACTCTTGGGGGTCTGAGGAGCGAAGTCTGCCCCGAAAAATGCCC
TTCGTCCGAGGCCAGAGCTTCTCGGTGTGGATCTTGTGTGAAGCTCACTGCCTCAAGGTG
GCCGTGGATGGTCAGCACGTGTTTGAATACTACCATCGCCTGAGGAACCTGCCCACCATC
AACAAACTGGAAGTGGGTGGCGACATCCAGCTGACCCACGTGCAGACA


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : LGALS9C
Symbol Nomenclature : LGALS9C
Designation : gal-9C|galectin 9 like|galectin-9-like protein B|galectin-9C|lectin, galactoside-binding, soluble, 9 (galectin 9) pseudogene
Full Nomenclature : lectin, galactoside-binding, soluble, 9C
GENEID : 654346
GenBank Accession : HQ448175
HGNC : 33874
Vega : OTTHUMG00000059251
Target GenBank: HQ448175

 

Reference Sequence Alignment

  

HsCD00439967        ATGGCCTTCAGCGGTTGCCAGGCTCCCTATCTGAGCCCAGCCGTCCCCTTTTCTGGGACT
NM_009587.2         ATGGCCTTCAGCGGTTCCCAGGCTCCCTACCTGAGTCCAGCTGTCCCCTTTTCTGGGACT
NM_001040078.2      ATGGCCTTCAGCGGTTGCCAGGCTCCCTATCTGAGCCCAGCCGTCCCCTTTTCTGGGACT
XM_005256769.1      ATGGCCTTCAGCGGTTGCCAGGCTCCCTATCTGAGCCCAGCCGTCCCCTTTTCTGGGACT
XM_005256770.1      ATGGCCTTCAGCGGTTGCCAGGCTCCCTATCTGAGCCCAGCCGTCCCCTTTTCTGGGACT
NM_001042685.1      ATGGCCTTCAGCGGTTCCCAGGCTCCCTATCTGAGCCCAGCCGTCCCCTTTTCTGGGACT
                    **************** ************ ***** ***** ******************

HsCD00439967        ATCCAAGGGGGTCTCCAGGACGGATTTCAGATCACTGTCAATGGGGCCGTTCTCAGCTGC
NM_009587.2         ATTCAAGGAGGTCTCCAGGACGGACTTCAGATCACTGTCAATGGGACCGTTCTCAGCTCC
NM_001040078.2      ATCCAAGGGGGTCTCCAGGACGGATTTCAGATCACTGTCAATGGGGCCGTTCTCAGCTGC
XM_005256769.1      ATCCAAGGGGGTCTCCAGGACGGATTTCAGATCACTGTCAATGGGGCCGTTCTCAGCTGC
XM_005256770.1      ATCCAAGGGGGTCTCCAGGACGGATTTCAGATCACTGTCAATGGGGCCGTTCTCAGCTGC
NM_001042685.1      ATCCAAGGGGGTCTCCAGGACGGATTTCAGATCACTGTCAATGGGGCCGTTCTCAGCTCC
                    ** *****.*************** ********************.************ *

HsCD00439967        AGTGGAACCAGGTTTGCTGTGGACTTTCAGACGGGCTTCAGTGGAAACGACATTGCCTTC
NM_009587.2         AGTGGAACCAGGTTTGCTGTGAACTTTCAGACTGGCTTCAGTGGAAATGACATTGCCTTC
NM_001040078.2      AGTGGAACCAGGTTTGCTGTGGACTTTCAGACGGGCTTCAGTGGAAACGACATTGCCTTC
XM_005256769.1      AGTGGAACCAGGTTTGCTGTGGACTTTCAGACGGGCTTCAGTGGAAACGACATTGCCTTC
XM_005256770.1      AGTGGAACCAGGTTTGCTGTGGACTTTCAGACGGGCTTCAGTGGAAACGACATTGCCTTC
NM_001042685.1      AGTGGAACCAGGTTTGCTGTGGACTTTCAGACGGGCTTCAGTGGAAACGACATTGCCTTC
                    *********************.********** ************** ************

HsCD00439967        CACTTCAACCCTCGGTTTGAAGACGGAGGGTATGTGGTGTGCAACACGAGGCAGAAAGGA
NM_009587.2         CACTTCAACCCTCGGTTTGAAGATGGAGGGTACGTGGTGTGCAACACGAGGCAGAACGGA
NM_001040078.2      CACTTCAACCCTCGGTTTGAAGACGGAGGGTATGTGGTGTGCAACACGAGGCAGAAAGGA
XM_005256769.1      CACTTCAACCCTCGGTTTGAAGACGGAGGGTATGTGGTGTGCAACACGAGGCAGAAAGGA
XM_005256770.1      CACTTCAACCCTCGGTTTGAAGACGGAGGGTATGTGGTGTGCAACACGAGGCAGAAAGGA
NM_001042685.1      CACTTCAACCCTCGGTTTGAAGACGGAGGGTATGTGGTGTGCAACACGAGGCAGAAAGGA
                    *********************** ******** ***********************.***

HsCD00439967        ACATGGGGGCCCGAGGAGAGGAAGATGCACATGCCCTTCCAGAAGGGGATGCCCTTTGAC
NM_009587.2         AGCTGGGGGCCCGAGGAGAGGAAGACACACATGCCTTTCCAGAAGGGGATGCCCTTTGAC
NM_001040078.2      ACATGGGGGCCCGAGGAGAGGAAGATGCACATGCCCTTCCAGAAGGGGATGCCCTTTGAC
XM_005256769.1      ACATGGGGGCCCGAGGAGAGGAAGATGCACATGCCCTTCCAGAAGGGGATGCCCTTTGAC
XM_005256770.1      ACATGGGGGCCCGAGGAGAGGAAGATGCACATGCCCTTCCAGAAGGGGATGCCCTTTGAC
NM_001042685.1      AGATGGGGGCCCGAGGAGAGGAAGATGCACATGCCCTTCCAGAAGGGGATGCCCTTTGAC
                    * .********************** .******** ************************

HsCD00439967        CTCTGCTTCCTGGTGCAGAGCTCAGATTTCAAGGTGATGGTGAACGGGAGCCTCTTCGTG
NM_009587.2         CTCTGCTTCCTGGTGCAGAGCTCAGATTTCAAGGTGATGGTGAACGGGATCCTCTTCGTG
NM_001040078.2      CTCTGCTTCCTGGTGCAGAGCTCAGATTTCAAGGTGATGGTGAACGGGAGCCTCTTCGTG
XM_005256769.1      CTCTGCTTCCTGGTGCAGAGCTCAGATTTCAAGGTGATGGTGAACGGGAGCCTCTTCGTG
XM_005256770.1      CTCTGCTTCCTGGTGCAGAGCTCAGATTTCAAGGTGATGGTGAACGGGAGCCTCTTCGTG
NM_001042685.1      CTCTGCTTCCTGGTGCAGAGCTCAGATTTCAAGGTGATGGTGAACGGGAGCCTCTTCGTG
                    ************************************************* **********

HsCD00439967        CAGTACTTCCACCGCGTGCCCTTCCACCGTGTGGACACCATCTCCGTCAATGGCTCTGTG
NM_009587.2         CAGTACTTCCACCGCGTGCCCTTCCACCGTGTGGACACCATCTCCGTCAATGGCTCTGTG
NM_001040078.2      CAGTACTTCCACCGCGTGCCCTTCCACCGTGTGGACACCATCTCCGTCAATGGCTCTGTG
XM_005256769.1      CAGTACTTCCACCGCGTGCCCTTCCACCGTGTGGACACCATCTCCGTCAATGGCTCTGTG
XM_005256770.1      CAGTACTTCCACCGCGTGCCCTTCCACCGTGTGGACACCATCTCCGTCAATGGCTCTGTG
NM_001042685.1      CAGTACTTCCACCGCGTGCCCTTCCACCGTGTGGACACCATCTCCGTCAATGGCTCTGTG
                    ************************************************************

HsCD00439967        CAGCTGTCCTACATCAGCTTCCAGAATCCCCGCGCAGTCCCCGTTCAGCCTGCCTTCTCC
NM_009587.2         CAGCTGTCCTACATCAGCTTCCAGAACCCCCGCACAGTCCCTGTTCAGCCTGCCTTCTCC
NM_001040078.2      CAGCTGTCCTACATCAGCTTCCAGAATCCCCGCGCAGTCCCCGTTCAGCCTGCCTTCTCC
XM_005256769.1      CAGCTGTCCTACATCAGCTTCCAGAATCCCCGCGCAGTCCCCGTTCAGCCTGCCTTCTCC
XM_005256770.1      CAGCTGTCCTACATCAGCTTCCAGCCTCCCAGCGTG------------------------
NM_001042685.1      CAGCTGTCCTACATCAGCTTCCAGAATCCCCGCACAGTCCCCGTTCAGCCTGCCTTCTCC
                    ************************.. ***.**. .                        

HsCD00439967        ACGGTGCCGTTCTCCCAGCCTGTCTGTTTCCCACCCAGGCCCAGGGGGCGCAGACAAAAA
NM_009587.2         ACGGTGCCGTTCTCCCAGCCTGTCTGTTTCCCACCCAGGCCCAGGGGGCGCAGACAAAAA
NM_001040078.2      ACGGTGCCGTTCTCCCAGCCTGTCTGTTTCCCACCCAGGCCCAGGGGGCGCAGACAAAAA
XM_005256769.1      ACGGTGCCGTTCTCCCAGCCTGTCTGTTTCCCACCCAGGCCCAGGGGGCGCAGACAAAAA
XM_005256770.1      ------------------------------------------------------------
NM_001042685.1      ACGGTGCCGTTCTCCCAGCCTGTCTGTTTCCCACCCAGGCCCAGGGGGCGCAGACAAAAA
                                                                                

HsCD00439967        CCTCCCATCGTGCGGCCTGCCAACCCAGCTCCCATTACCCAGACAGTCATCCACACGGTG
NM_009587.2         CCTCCCGGCGTGTGGCCTGCCAACCCGGCTCCCATTACCCAGACAGTCATCCACACAGTG
NM_001040078.2      CCTCCCAGCGTGCGGCCTGCCAACCCAGCTCCCATTACCCAGACAGTCATCCACACGGTG
XM_005256769.1      CCTCCCAGCGTGCGGCCTGCCAACCCAGCTCCCATTACCCAGACAGTCATCCACACGGTG
XM_005256770.1      ------------CGGCCTGCCAACCCAGCTCCCATTACCCAGACAGTCATCCACACGGTG
NM_001042685.1      CCTCCCAGCGTGCGGCCTGCCAACCCAGCTCCCATTACCCAGACAGTCATCCACACGGTG
                                 *************.*****************************.***

HsCD00439967        CAGAGTGCCTCTGGACAGATGTTCTCTCAGACTCCCGCCATCCCACCTATGATGTACCCC
NM_009587.2         CAGAGCGCCCCTGGACAGATGTTCTCTACT---CCCGCCATCCCACCTATGATGTACCCC
NM_001040078.2      CAGAGTGCCTCTGGACAGATGTTCTCTCAGACTCCCGCCATCCCACCTATGATGTACCCC
XM_005256769.1      CAGAGTGCCTCTGGACAGATGTTCTCTAC---TCCCGCCATCCCACCTATGATGTACCCC
XM_005256770.1      CAGAGTGCCTCTGGACAGATGTTCTCTAC---TCCCGCCATCCCACCTATGATGTACCCC
NM_001042685.1      CAGAGCGCCTCTGGACAGATGTTCTCTACT---CCCGCCATCCCACCTATGATGTACCCC
                    ***** *** *****************..    ***************************

HsCD00439967        CACCCTGCCTATCCGATGCCTTTCATCACCACCATTCCGGGAGGGCTGTACCCATCCAAG
NM_009587.2         CACCCCGCCTATCCGATGCCTTTCATCACCACCATTCTGGGAGGGCTGTACCCATCCAAG
NM_001040078.2      CACCCTGCCTATCCGATGCCTTTCATCACCACCATTCCGGGAGGGCTGTACCCATCCAAG
XM_005256769.1      CACCCTGCCTATCCGATGCCTTTCATCACCACCATTCCGGGAGGGCTGTACCCATCCAAG
XM_005256770.1      CACCCTGCCTATCCGATGCCTTTCATCACCACCATTCCGGGAGGGCTGTACCCATCCAAG
NM_001042685.1      CACCCTGCCTATCCGATGCCTTTCATCACCACCATTCCGGGAGGGCTGTACCCATCCAAG
                    ***** ******************************* **********************

HsCD00439967        TCCATCATCCTGTCAGGCACTGTCCTGCCCAGTGCTCAGAGGTTCCACATCAACCTGTGC
NM_009587.2         TCCATCCTCCTGTCAGGCACTGTCCTGCCCAGTGCTCAGAGGTTCCACATCAACCTGTGC
NM_001040078.2      TCCATCATCCTGTCAGGCACTGTCCTGCCCAGTGCTCAGAGGTTCCACATCAACCTGTGC
XM_005256769.1      TCCATCATCCTGTCAGGCACTGTCCTGCCCAGTGCTCAGAGGTTCCACATCAACCTGTGC
XM_005256770.1      TCCATCATCCTGTCAGGCACTGTCCTGCCCAGTGCTCAGAGGTTCCACATCAACCTGTGC
NM_001042685.1      TCCATCATCCTGTCAGGCACTGTCCTGCCCAGTGCTCAGAGGTTCCACATCAACCTGTGC
                    ******.*****************************************************

HsCD00439967        TCTGGGAGCCACATCGCCTTCCACATGAACCCCCGTTTTGATGAGAATGCTGTGGTCCGT
NM_009587.2         TCTGGGAACCACATCGCCTTCCACCTGAACCCCCGTTTTGATGAGAATGCTGTGGTCCGC
NM_001040078.2      TCTGGGAGCCACATCGCCTTCCACATGAACCCCCGTTTTGATGAGAATGCTGTGGTCCGT
XM_005256769.1      TCTGGGAGCCACATCGCCTTCCACATGAACCCCCGTTTTGATGAGAATGCTGTGGTCCGT
XM_005256770.1      TCTGGGAGCCACATCGCCTTCCACATGAACCCCCGTTTTGATGAGAATGCTGTGGTCCGT
NM_001042685.1      TCTGGGAGCCACATCGCCTTCCACATGAACCCCCGTTTTGATGAGAATGCTGTGGTCCGT
                    *******.****************.********************************** 

HsCD00439967        AACACCCAGATCAACAACTCTTGGGGGTCTGAGGAGCGAAGTCTGCCCCGAAAAATGCCC
NM_009587.2         AACACCCAGATCGACAACTCCTGGGGGTCTGAGGAGCGAAGTCTGCCCCGAAAAATGCCC
NM_001040078.2      AACACCCAGATCAACAACTCTTGGGGGTCTGAGGAGCGAAGTCTGCCCCGAAAAATGCCC
XM_005256769.1      AACACCCAGATCAACAACTCTTGGGGGTCTGAGGAGCGAAGTCTGCCCCGAAAAATGCCC
XM_005256770.1      AACACCCAGATCAACAACTCTTGGGGGTCTGAGGAGCGAAGTCTGCCCCGAAAAATGCCC
NM_001042685.1      AACACCCAGATCAACAACTCTTGGGGGTCTGAGGAGCGAAGTCTGCCCCGAAAAATGCCC
                    ************.******* ***************************************

HsCD00439967        TTCGTCCGAGGCCAGAGCTTCTCGGTGTGGATCTTGTGTGAAGCTCACTGCCTCAAGGTG
NM_009587.2         TTCGTCCGTGGCCAGAGCTTCTCAGTGTGGATCTTGTGTGAAGCTCACTGCCTCAAGGTG
NM_001040078.2      TTCGTCCGAGGCCAGAGCTTCTCGGTGTGGATCTTGTGTGAAGCTCACTGCCTCAAGGTG
XM_005256769.1      TTCGTCCGAGGCCAGAGCTTCTCGGTGTGGATCTTGTGTGAAGCTCACTGCCTCAAGGTG
XM_005256770.1      TTCGTCCGAGGCCAGAGCTTCTCGGTGTGGATCTTGTGTGAAGCTCACTGCCTCAAGGTG
NM_001042685.1      TTCGTCCGAGGCCAGAGCTTCTCGGTGTGGATCTTGTGTGAAGCTCACTGCCTCAAGGTG
                    ********:**************.************************************

HsCD00439967        GCCGTGGATGGTCAGCACGTGTTTGAATACTACCATCGCCTGAGGAACCTGCCCACCATC
NM_009587.2         GCCGTGGATGGTCAGCACCTGTTTGAATACTACCATCGCCTGAGGAACCTGCCCACCATC
NM_001040078.2      GCCGTGGATGGTCAGCACGTGTTTGAATACTACCATCGCCTGAGGAACCTGCCCACCATC
XM_005256769.1      GCCGTGGATGGTCAGCACGTGTTTGAATACTACCATCGCCTGAGGAACCTGCCCACCATC
XM_005256770.1      GCCGTGGATGGTCAGCACGTGTTTGAATACTACCATCGCCTGAGGAACCTGCCCACCATC
NM_001042685.1      GCCGTGGATGGTCAGCACGTGTTTGAATACTACCATCGCCTGAGGAACCTGCCCACCATC
                    ****************** *****************************************

HsCD00439967        AACAAACTGGAAGTGGGTGGCGACATCCAGCTGACCCACGTGCAGACA---
NM_009587.2         AACAGACTGGAAGTGGGGGGCGACATCCAGCTGACCCATGTGCAGACATAG
NM_001040078.2      AACAAACTGGAAGTGGGTGGCGACATCCAGCTGACCCACGTGCAGACATAG
XM_005256769.1      AACAAACTGGAAGTGGGTGGCGACATCCAGCTGACCCACGTGCAGACATAG
XM_005256770.1      AACAAACTGGAAGTGGGTGGCGACATCCAGCTGACCCACGTGCAGACATAG
NM_001042685.1      AACAAACTGGAAGTGGGTGGCGACATCCAGCTGACCCACGTGCAGACATAG
                    ****.************ ******************** *********   

Clone

Original Clone ID : ccsbBroad304_10195

Organism


TAX_ID : 9606
Species Specific ID: 654346

Genome

Chromosome : 17
Map Location : 17p11.2
Ensembl : ENSG00000171916

Reagents

Labome : LGALS9C-antibody