DNASU Plasmid Repository • 480.965.5697 | Email

NPFFR1 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  NPFFR1
Gene Name:  neuropeptide FF receptor 1
Sequence             
Original Clone ID: ccsbBroad304_08834
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 23430259
Title: Role of C-terminal Membrane-Proximal Basic Residues in Cell Surface Trafficking of HIV Coreceptor GPR15
PMID: 23430259
Title: Role of C-terminal Membrane-Proximal Basic Residues in Cell Surface Trafficking of HIV Coreceptor GPR15
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00440316

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1291nts         Open reading frame : 1 to 1291
Reference Sequence Alignment

ATGGAGGGGGAGCCCTCCCAGCCTCCCAACAGCAGTTGGCCCCTAAGTCAGAATGGGACT
AACACTGAGGCCACCCCGGCTACAAACCTCACCTTCTCCTCCTACTATCAGCACACCTCC
CCTGTGGCGGCCATGTTCATTGTGGCCTATGCGCTCATCTTCCTGCTCTGCATGGTGGGC
AACACCCTGGTCTGTTTCATCGTGCTCAAGAACCGGCACATGCATACTGTCACCAACATG
TTCATCCTCAACCTGGCTGTCAGTGACCTGCTGGTGGGCATCTTCTGCATGCCCACCACC
CTTGTGGACAACCTCATCACTGGGTGGCCCTTCGACAATGCCACATGCAAGATGAGCGGC
TTGGTGCAGGGCATGTCTGTGTCGGCTTCCGTTTTCACACTGGTGGCCATTGCTGTGGAA
AGGTTCCGCTGCATCGTGCACCCTTTCCGCGAGAAGCTGACCCTGCGGAAGGCGCTCGTC
ACCATCGCCGTCATCTGGGCCCTGGCGCTGCTCATCATGTGTCCCTCGGCCGTCACGCTG
ACCGTCACCCGTGAGGAGCACCACTTCATGGTGGACGCCCGCAACCGCTCCTACCCTCTC
TACTCCTGCTGGGAGGCCTGGCCCGAGAAGGGCATGCGCAGGGTCTACACCACTGTGCTC
TTCTCGCACATCTACCTGGCGCCGCTGGCGCTCATCGTGGTCATGTACGCCCGCATCGCG
CGCAAGCTCTGCCAGGCCCCGGGCCCGGCCCCCGGGGGCGAGGAGGCTGCGGACCCGCGA
GCATCGCGGCGCAGAGCGCGCGTGGTGCACATGCTGGTCATGGTGGCGCTGTTCTTCACG
CTGTCCTGGCTGCCGCTCTGGGCGCTGCTGCTGCTCATCGACTACGGGCAGCTCAGCGCG
CCGCAGCTGCACCTGGTCACCGTCTACGCCTTCCCCTTCGCGCACTGGCTGGCCTTCTTC
AACAGCAGCGCCAACCCCATCATCTACGGCTACTTCAACGAGAACTTCCGCCGCGGCTTC
CAGGCCGCCTTCCGCGCCCGCCTCTGCCCGCGCCCGTCGGGGAGCCACAAGGAGGCCTAC
TCCGAGCGGCCCGGCGGGCTTCTGCACAGGCGGGTCTTCGTGGTGGTGCGGCCCAGCGAC
TCCGGGCTGCCCTCTGAGTCGGGCCCTAGCAGTGGGGCCCCCAGGCCCGGCCGCCTCCCG
CTGCGGAATGGGCGGGTGGCTCACCACGGCTTGCCCAGGGAAGGGCCTGGCTGCTCCCAC
CTGCCCCTCACCATTCCAGCCTGGGATATC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : NPFFR1
Symbol Nomenclature : NPFFR1
SYNONYM : GPR147; NPFF1; NPFF1R1; OT7T022
Designation : G protein-coupled receptor 147|G-protein coupled receptor 147|RFamide-related peptide receptor OT7T022|neuropeptide FF 1
Full Nomenclature : neuropeptide FF receptor 1
GENEID : 64106
Locus Tag : RP11-203J14.1
HGNC : 17425
MIM : 607448
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00440316      ATGGAGGGGGAGCCCTCCCAGCCTCCCAACAGCAGTTGGCCCCTAAGTCAGAATGGGACT
NM_022146.4       ATGGAGGGGGAGCCCTCCCAGCCTCCCAACAGCAGTTGGCCCCTAAGTCAGAATGGGACT
                  ************************************************************

HsCD00440316      AACACTGAGGCCACCCCGGCTACAAACCTCACCTTCTCCTCCTACTATCAGCACACCTCC
NM_022146.4       AACACTGAGGCCACCCCGGCTACAAACCTCACCTTCTCCTCCTACTATCAGCACACCTCC
                  ************************************************************

HsCD00440316      CCTGTGGCGGCCATGTTCATTGTGGCCTATGCGCTCATCTTCCTGCTCTGCATGGTGGGC
NM_022146.4       CCTGTGGCGGCCATGTTCATTGTGGCCTATGCGCTCATCTTCCTGCTCTGCATGGTGGGC
                  ************************************************************

HsCD00440316      AACACCCTGGTCTGTTTCATCGTGCTCAAGAACCGGCACATGCATACTGTCACCAACATG
NM_022146.4       AACACCCTGGTCTGTTTCATCGTGCTCAAGAACCGGCACATGCATACTGTCACCAACATG
                  ************************************************************

HsCD00440316      TTCATCCTCAACCTGGCTGTCAGTGACCTGCTGGTGGGCATCTTCTGCATGCCCACCACC
NM_022146.4       TTCATCCTCAACCTGGCTGTCAGTGACCTGCTGGTGGGCATCTTCTGCATGCCCACCACC
                  ************************************************************

HsCD00440316      CTTGTGGACAACCTCATCACTGGGTGGCCCTTCGACAATGCCACATGCAAGATGAGCGGC
NM_022146.4       CTTGTGGACAACCTCATCACTGGGTGGCCCTTCGACAATGCCACATGCAAGATGAGCGGC
                  ************************************************************

HsCD00440316      TTGGTGCAGGGCATGTCTGTGTCGGCTTCCGTTTTCACACTGGTGGCCATTGCTGTGGAA
NM_022146.4       TTGGTGCAGGGCATGTCTGTGTCGGCTTCCGTTTTCACACTGGTGGCCATTGCTGTGGAA
                  ************************************************************

HsCD00440316      AGGTTCCGCTGCATCGTGCACCCTTTCCGCGAGAAGCTGACCCTGCGGAAGGCGCTCGTC
NM_022146.4       AGGTTCCGCTGCATCGTGCACCCTTTCCGCGAGAAGCTGACCCTGCGGAAGGCGCTCGTC
                  ************************************************************

HsCD00440316      ACCATCGCCGTCATCTGGGCCCTGGCGCTGCTCATCATGTGTCCCTCGGCCGTCACGCTG
NM_022146.4       ACCATCGCCGTCATCTGGGCCCTGGCGCTGCTCATCATGTGTCCCTCGGCCGTCACGCTG
                  ************************************************************

HsCD00440316      ACCGTCACCCGTGAGGAGCACCACTTCATGGTGGACGCCCGCAACCGCTCCTACCCTCTC
NM_022146.4       ACCGTCACCCGTGAGGAGCACCACTTCATGGTGGACGCCCGCAACCGCTCCTACCCGCTC
                  ******************************************************** ***

HsCD00440316      TACTCCTGCTGGGAGGCCTGGCCCGAGAAGGGCATGCGCAGGGTCTACACCACTGTGCTC
NM_022146.4       TACTCCTGCTGGGAGGCCTGGCCCGAGAAGGGCATGCGCAGGGTCTACACCACTGTGCTC
                  ************************************************************

HsCD00440316      TTCTCGCACATCTACCTGGCGCCGCTGGCGCTCATCGTGGTCATGTACGCCCGCATCGCG
NM_022146.4       TTCTCGCACATCTACCTGGCGCCGCTGGCGCTCATCGTGGTCATGTACGCCCGCATCGCG
                  ************************************************************

HsCD00440316      CGCAAGCTCTGCCAGGCCCCGGGCCCGGCCCCCGGGGGCGAGGAGGCTGCGGACCCGCGA
NM_022146.4       CGCAAGCTCTGCCAGGCCCCGGGCCCGGCCCCCGGGGGCGAGGAGGCTGCGGACCCGCGA
                  ************************************************************

HsCD00440316      GCATCGCGGCGCAGAGCGCGCGTGGTGCACATGCTGGTCATGGTGGCGCTGTTCTTCACG
NM_022146.4       GCATCGCGGCGCAGAGCGCGCGTGGTGCACATGCTGGTCATGGTGGCGCTGTTCTTCACG
                  ************************************************************

HsCD00440316      CTGTCCTGGCTGCCGCTCTGGGCGCTGCTGCTGCTCATCGACTACGGGCAGCTCAGCGCG
NM_022146.4       CTGTCCTGGCTGCCGCTCTGGGCGCTGCTGCTGCTCATCGACTACGGGCAGCTCAGCGCG
                  ************************************************************

HsCD00440316      CCGCAGCTGCACCTGGTCACCGTCTACGCCTTCCCCTTCGCGCACTGGCTGGCCTTCTTC
NM_022146.4       CCGCAGCTGCACCTGGTCACCGTCTACGCCTTCCCCTTCGCGCACTGGCTGGCCTTCTTC
                  ************************************************************

HsCD00440316      AACAGCAGCGCCAACCCCATCATCTACGGCTACTTCAACGAGAACTTCCGCCGCGGCTTC
NM_022146.4       AACAGCAGCGCCAACCCCATCATCTACGGCTACTTCAACGAGAACTTCCGCCGCGGCTTC
                  ************************************************************

HsCD00440316      CAGGCCGCCTTCCGCGCCCGCCTCTGCCCGCGCCCGTCGGGGAGCCACAAGGAGGCCTAC
NM_022146.4       CAGGCCGCCTTCCGCGCCCGCCTCTGCCCGCGCCCGTCGGGGAGCCACAAGGAGGCCTAC
                  ************************************************************

HsCD00440316      TCCGAGCGGCCCGGCGGGCTTCTGCACAGGCGGGTCTTCGTGGTGGTGCGGCCCAGCGAC
NM_022146.4       TCCGAGCGGCCCGGCGGGCTTCTGCACAGGCGGGTCTTCGTGGTGGTGCGGCCCAGCGAC
                  ************************************************************

HsCD00440316      TCCGGGCTGCCCTCTGAGTCGGGCCCTAGCAGTGGGGCCCCCAGGCCCGGCCGCCTCCCG
NM_022146.4       TCCGGGCTGCCCTCTGAGTCGGGCCCTAGCAGTGGGGCCCCCAGGCCCGGCCGCCTCCCG
                  ************************************************************

HsCD00440316      CTGCGGAATGGGCGGGTGGCTCACCACGGCTTGCCCAGGGAAGGGCCTGGCTGCTCCCAC
NM_022146.4       CTGCGGAATGGGCGGGTGGCTCACCACGGCTTGCCCAGGGAAGGGCCTGGCTGCTCCCAC
                  ************************************************************

HsCD00440316      CTGCCCCTCACCATTCCAGCCTGGGATATC---
NM_022146.4       CTGCCCCTCACCATTCCAGCCTGGGATATCTGA
                  ******************************   

Pathways

Reactome : Class A/1 (Rhodopsin-like receptors)
Reactome : G alpha (q) signalling events
Reactome : GPCR downstream signaling
Reactome : GPCR ligand binding
Reactome : Gastrin-CREB signalling pathway via PKC and MAPK
Reactome : Orexin and neuropeptides FF and QRFP bind to their respective receptors
Reactome : Peptide ligand-binding receptors
Reactome : Signal Transduction
Reactome : Signaling by GPCR
WikiPathway : GPCRs, Class A Rhodopsin-like

Protein

HPRD : 12120

Clone

Original Clone ID : ccsbBroad304_08834

Organism


TAX_ID : 9606
Species Specific ID: 64106

Genome

Chromosome : 10
Map Location : 10q21-q22

Reagents

Labome : NPFFR1-antibody