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DFFA (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  DFFA
Gene Name:  DNA fragmentation factor, 45kDa, alpha polypeptide
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH110789.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 16512675
Title: Functional proteomics approach to investigate the biological activities of cDNAs implicated in breast cancer.
Authors: HIP
HsCD00004404

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 996nts         Open reading frame : 1 to 996
Reference Sequence Alignment

ATGGAGGTGACCGGGGACGCCGGGGTACCAGAATCTGGCGAGATCCGGACTCTAAAGCCG
TGTCTGCTGCGCCGCAACTACAGCCGCGAACAGCACGGCGTGGCCGCCTCCTGCCTCGAA
GACCTGAGGAGCAAGGCCTGTGACATTCTGGCCATTGATAAGTCCCTGACACCAGTCACC
CTGGTCCTGGCAGAGGATGGCACCATAGTGGATGATGACGATTACTTTCTGTGTCTACCT
TCCAATACTAAGTTTGTGGCATTGGCTAGTAATGAGAAATGGGCATACAACAATTCAGAT
GGAGGTACAGCTTGGATTTCCCAAGAGTCCTTTGATGTAGATGAAACAGACAGCGGGGCA
GGGTTGAAGTGGAAGAATGTGGCCAGGCAGCTGAAAGAAGATCTGTCCAGCATCATCCTC
CTATCAGAGGAGGACCTCCAGATGCTTGTTGACGCTCCCTGCTCAGACCTGGCTCAGGAA
CTACGTCAGAGTTGTGCCACCGTCCAGCGGCTGCAGCACACACTCCAACAGGTGCTTGAC
CAAAGAGAGGAAGTGCGTCAGTCCAAGCAGCTCCTGCAGCTGTACCTCCAGGCTTTGGAG
AAAGAGGGCAGCCTCTTGTCAAAGCAGGAAGAGTCCAAAGCTGCCTTTGGTGAGGAGGTG
GATGCAGTAGACACGGGTATCAGCAGAGAGACCTCCTCGGACGTTGCGCTGGCGAGCCAC
ATCCTTACTGCACTGAGGGAGAAGCAGGCTCCAGAGCTGAGCTTATCTAGTCAGGATTTG
GAGTTGGTTACCAAGGAAGACCCCAAAGCACTGGCTGTTGCCTTGAACTGGGACATAAAG
AAGACGGAGACTGTTCAGGAGGCCTGTGAGCGGGAGCTCGCCCTGCGCCTGCAGCAGACG
CAGAGCTTGCATTCTCTCCGGAGCATCTCAGCAAGCAAGGCCTCACCACCTGGTGACCTG
CAGAATCCTAAGCGAGCCAGACAGGATCCCACATTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1065
Start on reference sequence 70
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : DFFA
Symbol Nomenclature : DFFA
SYNONYM : DFF-45; DFF1; ICAD
Designation : DFF45|DNA fragmentation factor 45 kDa subunit|DNA fragmentation factor subunit alpha|inhibitor of CAD
Full Nomenclature : DNA fragmentation factor, 45kDa, alpha polypeptide
GENEID : 1676
Locus Tag : H13
GI : 60653666
GenBank Accession : AY892610
HGNC : 2772
MIM : 601882
Target GenBank: BC007112

 

Reference Sequence Alignment

  

HsCD00004404      ATGGAGGTGACCGGGGACGCCGGGGTACCAGAATCTGGCGAGATCCGGACTCTAAAGCCG
NM_004401.2       ATGGAGGTGACCGGGGACGCCGGGGTACCAGAATCTGGCGAGATCCGGACTCTAAAGCCG
                  ************************************************************

HsCD00004404      TGTCTGCTGCGCCGCAACTACAGCCGCGAACAGCACGGCGTGGCCGCCTCCTGCCTCGAA
NM_004401.2       TGTCTGCTGCGCCGCAACTACAGCCGCGAACAGCACGGCGTGGCCGCCTCCTGCCTCGAA
                  ************************************************************

HsCD00004404      GACCTGAGGAGCAAGGCCTGTGACATTCTGGCCATTGATAAGTCCCTGACACCAGTCACC
NM_004401.2       GACCTGAGGAGCAAGGCCTGTGACATTCTGGCCATTGATAAGTCCCTGACACCAGTCACC
                  ************************************************************

HsCD00004404      CTGGTCCTGGCAGAGGATGGCACCATAGTGGATGATGACGATTACTTTCTGTGTCTACCT
NM_004401.2       CTGGTCCTGGCAGAGGATGGCACCATAGTGGATGATGACGATTACTTTCTGTGTCTACCT
                  ************************************************************

HsCD00004404      TCCAATACTAAGTTTGTGGCATTGGCTAGTAATGAGAAATGGGCATACAACAATTCAGAT
NM_004401.2       TCCAATACTAAGTTTGTGGCATTGGCTAGTAATGAGAAATGGGCATACAACAATTCAGAT
                  ************************************************************

HsCD00004404      GGAGGTACAGCTTGGATTTCCCAAGAGTCCTTTGATGTAGATGAAACAGACAGCGGGGCA
NM_004401.2       GGAGGTACAGCTTGGATTTCCCAAGAGTCCTTTGATGTAGATGAAACAGACAGCGGGGCA
                  ************************************************************

HsCD00004404      GGGTTGAAGTGGAAGAATGTGGCCAGGCAGCTGAAAGAAGATCTGTCCAGCATCATCCTC
NM_004401.2       GGGTTGAAGTGGAAGAATGTGGCCAGGCAGCTGAAAGAAGATCTGTCCAGCATCATCCTC
                  ************************************************************

HsCD00004404      CTATCAGAGGAGGACCTCCAGATGCTTGTTGACGCTCCCTGCTCAGACCTGGCTCAGGAA
NM_004401.2       CTATCAGAGGAGGACCTCCAGATGCTTGTTGACGCTCCCTGCTCAGACCTGGCTCAGGAA
                  ************************************************************

HsCD00004404      CTACGTCAGAGTTGTGCCACCGTCCAGCGGCTGCAGCACACACTCCAACAGGTGCTTGAC
NM_004401.2       CTACGTCAGAGTTGTGCCACCGTCCAGCGGCTGCAGCACACACTCCAACAGGTGCTTGAC
                  ************************************************************

HsCD00004404      CAAAGAGAGGAAGTGCGTCAGTCCAAGCAGCTCCTGCAGCTGTACCTCCAGGCTTTGGAG
NM_004401.2       CAAAGAGAGGAAGTGCGTCAGTCCAAGCAGCTCCTGCAGCTGTACCTCCAGGCTTTGGAG
                  ************************************************************

HsCD00004404      AAAGAGGGCAGCCTCTTGTCAAAGCAGGAAGAGTCCAAAGCTGCCTTTGGTGAGGAGGTG
NM_004401.2       AAAGAGGGCAGCCTCTTGTCAAAGCAGGAAGAGTCCAAAGCTGCCTTTGGTGAGGAGGTG
                  ************************************************************

HsCD00004404      GATGCAGTAGACACGGGTATCAGCAGAGAGACCTCCTCGGACGTTGCGCTGGCGAGCCAC
NM_004401.2       GATGCAGTAGACACGGGTATCAGCAGAGAGACCTCCTCGGACGTTGCGCTGGCGAGCCAC
                  ************************************************************

HsCD00004404      ATCCTTACTGCACTGAGGGAGAAGCAGGCTCCAGAGCTGAGCTTATCTAGTCAGGATTTG
NM_004401.2       ATCCTTACTGCACTGAGGGAGAAGCAGGCTCCAGAGCTGAGCTTATCTAGTCAGGATTTG
                  ************************************************************

HsCD00004404      GAGTTGGTTACCAAGGAAGACCCCAAAGCACTGGCTGTTGCCTTGAACTGGGACATAAAG
NM_004401.2       GAGTTGGTTACCAAGGAAGACCCCAAAGCACTGGCTGTTGCCTTGAACTGGGACATAAAG
                  ************************************************************

HsCD00004404      AAGACGGAGACTGTTCAGGAGGCCTGTGAGCGGGAGCTCGCCCTGCGCCTGCAGCAGACG
NM_004401.2       AAGACGGAGACTGTTCAGGAGGCCTGTGAGCGGGAGCTCGCCCTGCGCCTGCAGCAGACG
                  ************************************************************

HsCD00004404      CAGAGCTTGCATTCTCTCCGGAGCATCTCAGCAAGCAAGGCCTCACCACCTGGTGACCTG
NM_004401.2       CAGAGCTTGCATTCTCTCCGGAGCATCTCAGCAAGCAAGGCCTCACCACCTGGTGACCTG
                  ************************************************************

HsCD00004404      CAGAATCCTAAGCGAGCCAGACAGGATCCCACATTG
NM_004401.2       CAGAATCCTAAGCGAGCCAGACAGGATCCCACATAG
                  **********************************:*

Pathways

NCI : Caspase Cascade in Apoptosis
NCI : HIV-1 Nef: Negative effector of Fas and TNF-alpha
Reactome : Activation of DNA fragmentation factor
Reactome : Apoptosis
Reactome : Apoptosis induced DNA fragmentation
Reactome : Apoptotic execution phase
WikiPathway : Apoptosis
WikiPathway : Apoptosis Modulation and Signaling
WikiPathway : FAS pathway and Stress induction of HSP regulation

Clone

Cloning Information : 110789
HIP Master Clone ID : 87072
Original Clone ID : FLH110789.01L

Organism


TAX_ID : 9606
Species Specific ID: 1676

Genome

Chromosome : 1
Map Location : 1p36.3-p36.2

Reagents

Labome : DFF45-antibody