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MRPL45 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  MRPL45
Gene Name:  mitochondrial ribosomal protein L45
Sequence             
Original Clone ID: ccsbBroad304_09180
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00440452

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 919nts         Open reading frame : 1 to 919
Reference Sequence Alignment

ATGGCAGCCCCCATACCTCAAGGGTTCTCTTGTTTATCGAGGTTTTTGGGCTGGTGGTTT
CGGCAGCCAGTTCTGGTGACTCAGTCCGCAGCTATAGTTCCAGTAAGAACTAAAAAACGT
TTCACACCTCCTATTTATCAACCTAAATTTAAAACAGAAAAGGAGTTTATGCAACATGCC
CGGAAAGCAGGATTGGTTATTCCTCCAGAAAAATCGGACCGTTCCATACATCTGGCCTGT
ACAGCTGGTATATTTGATGCCTATGTTCCTCCTGAGGGTGATGCACGCATATCATCTCTT
TCAAAGGAGGGACTGATAGAGAGAACTGAACGAATGAAGAAGACTATGGCATCACAAGTG
TCAATCCGGAGGATAAAAGACTATGATGCCAACTTTAAAATAAAGGACTTCCCTGAAAAA
GCTAAGGATATCTTTATTGAAGCTCACCTTTGTCTAAATAACTCAGACCATGACCGACTT
CATACCTTGGTAACTGAACACTGTTTTCCAGACATGACTTGGGACATCAAATATAAGACC
GTCCGCTGGAGCTTTGTGGAATCTTTAGAGCCCTCTCATGTTGTTCAAGTTCGCTGTTCA
AGTATGATGAACCAGGGCAACGTGTACGGCCAGATCACCGTACGCATGCACACCCGGCAG
ACTCTGGCCATCTATGACCGGTTTGGCCGGTTGATGTATGGACAGGAAGATGTACCCAAG
GATGTCCTGGAGTATGTTGTATTCGAAAAGCAGTTGACAAACCCCTATGGAAGCTGGAGA
ATGCATACCAAGATCGTTCCCCCATGGGCACCCCCTAAGCAGCCCATCCTTAAGACGGTG
ATGATCCCTGGCCCTCAGCTGAAACCAGAAGAAGAATATGAAGAGGCACAAGGAGAGGCC
CAGAAGCCTCAGCTAGCC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : MRPL45
Symbol Nomenclature : MRPL45
SYNONYM : L45mt; MRP-L45
Designation : 39S ribosomal protein L45, mitochondrial
Full Nomenclature : mitochondrial ribosomal protein L45
GENEID : 84311
GenBank Accession : HQ258365
HGNC : 16651
MIM : 611850
Vega : OTTHUMG00000133120
Target GenBank: HQ258365

 

Reference Sequence Alignment

  

HsCD00440452      ATGGCAGCCCCCATACCTCAAGGGTTCTCTTGTTTATCGAGGTTTTTGGGCTGGTGGTTT
NM_032351.4       ATGGCAGCCCCCATACCTCAAGGGTTCTCTTGTTTATCGAGGTTTTTGGGCTGGTGGTTT
                  ************************************************************

HsCD00440452      CGGCAGCCAGTTCTGGTGACTCAGTCCGCAGCTATAGTTCCAGTAAGAACTAAAAAACGT
NM_032351.4       CGGCAGCCAGTTCTGGTGACTCAGTCCGCAGCTATAGTTCCAGTAAGAACTAAAAAACGT
                  ************************************************************

HsCD00440452      TTCACACCTCCTATTTATCAACCTAAATTTAAAACAGAAAAGGAGTTTATGCAACATGCC
NM_032351.4       TTCACACCTCCTATTTATCAACCTAAATTTAAAACAGAAAAGGAGTTTATGCAACATGCC
                  ************************************************************

HsCD00440452      CGGAAAGCAGGATTGGTTATTCCTCCAGAAAAATCGGACCGTTCCATACATCTGGCCTGT
NM_032351.4       CGGAAAGCAGGATTGGTTATTCCTCCAGAAAAATCGGACCGTTCCATACATCTGGCCTGT
                  ************************************************************

HsCD00440452      ACAGCTGGTATATTTGATGCCTATGTTCCTCCTGAGGGTGATGCACGCATATCATCTCTT
NM_032351.4       ACAGCTGGTATATTTGATGCCTATGTTCCTCCTGAGGGTGATGCACGCATATCATCTCTT
                  ************************************************************

HsCD00440452      TCAAAGGAGGGACTGATAGAGAGAACTGAACGAATGAAGAAGACTATGGCATCACAAGTG
NM_032351.4       TCAAAGGAGGGACTGATAGAGAGAACTGAACGAATGAAGAAGACTATGGCATCACAAGTG
                  ************************************************************

HsCD00440452      TCAATCCGGAGGATAAAAGACTATGATGCCAACTTTAAAATAAAGGACTTCCCTGAAAAA
NM_032351.4       TCAATCCGGAGGATAAAAGACTATGATGCCAACTTTAAAATAAAGGACTTCCCTGAAAAA
                  ************************************************************

HsCD00440452      GCTAAGGATATCTTTATTGAAGCTCACCTTTGTCTAAATAACTCAGACCATGACCGACTT
NM_032351.4       GCTAAGGATATCTTTATTGAAGCTCACCTTTGTCTAAATAACTCAGACCATGACCGACTT
                  ************************************************************

HsCD00440452      CATACCTTGGTAACTGAACACTGTTTTCCAGACATGACTTGGGACATCAAATATAAGACC
NM_032351.4       CATACCTTGGTAACTGAACACTGTTTTCCAGACATGACTTGGGACATCAAATATAAGACC
                  ************************************************************

HsCD00440452      GTCCGCTGGAGCTTTGTGGAATCTTTAGAGCCCTCTCATGTTGTTCAAGTTCGCTGTTCA
NM_032351.4       GTCCGCTGGAGCTTTGTGGAATCTTTAGAGCCCTCTCATGTTGTTCAAGTTCGCTGTTCA
                  ************************************************************

HsCD00440452      AGTATGATGAACCAGGGCAACGTGTACGGCCAGATCACCGTACGCATGCACACCCGGCAG
NM_032351.4       AGTATGATGAACCAGGGCAACGTGTACGGCCAGATCACCGTACGCATGCACACCCGGCAG
                  ************************************************************

HsCD00440452      ACTCTGGCCATCTATGACCGGTTTGGCCGGTTGATGTATGGACAGGAAGATGTACCCAAG
NM_032351.4       ACTCTGGCCATCTATGACCGGTTTGGCCGGTTGATGTATGGACAGGAAGATGTACCCAAG
                  ************************************************************

HsCD00440452      GATGTCCTGGAGTATGTTGTATTCGAAAAGCAGTTGACAAACCCCTATGGAAGCTGGAGA
NM_032351.4       GATGTCCTGGAGTATGTTGTATTCGAAAAGCAGTTGACAAACCCCTATGGAAGCTGGAGA
                  ************************************************************

HsCD00440452      ATGCATACCAAGATCGTTCCCCCATGGGCACCCCCTAAGCAGCCCATCCTTAAGACGGTG
NM_032351.4       ATGCATACCAAGATCGTTCCCCCATGGGCACCCCCTAAGCAGCCCATCCTTAAGACGGTG
                  ************************************************************

HsCD00440452      ATGATCCCTGGCCCTCAGCTGAAACCAGAAGAAGAATATGAAGAGGCACAAGGAGAGGCC
NM_032351.4       ATGATCCCTGGCCCTCAGCTGAAACCAGAAGAAGAATATGAAGAGGCACAAGGAGAGGCC
                  ************************************************************

HsCD00440452      CAGAAGCCTCAGCTAGCC---
NM_032351.4       CAGAAGCCTCAGCTAGCCTGA
                  ******************   

Protein

SMART domain : Tim44 :
HPRD : 14764

Clone

Original Clone ID : ccsbBroad304_09180

Organism


TAX_ID : 9606
Species Specific ID: 84311

Genome

Chromosome : 17
Map Location : 17q21.2
Ensembl : ENSG00000174100

Reagents

Labome : MRPL45-antibody