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RBM17 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  RBM17
Gene Name:  RNA binding motif protein 17
Sequence             
Original Clone ID: ccsbBroad304_14325
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00440492

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1203nts         Open reading frame : 1 to 1203
Reference Sequence Alignment

ATGTCCCTGTACGATGACCTAGGAGTGGAGACCAGTGACTCAAAAACAGAAGGCTGGTCC
AAAAACTTCAAACTTCTGCAGTCTCAGCTTCAGGTGAAGAAGGCAGCTCTCACTCAGGCA
AAGAGCCAAAGGACGAAACAAAGTACAGTCCTCGCCCCAGTCATTGACCTGAAGCGAGGT
GGCTCCTCAGATGACCGGCAAATTGTGGACACTCCACCGCATGTAGCAGCTGGGCTGAAG
GATCCTGTTCCCAGTGGGTTTTCTGCAGGGGAAGTTCTGATTCCCTTAGCTGACGAATAT
GACCCTATGTTTCCTAATGATTATGAGAAAGTAGTGAAGCGCCAAAGAGAGGAACGACAG
AGACAGCGGGAGCTGGAAAGACAAAAGGAAATAGAAGAAAGGGAAAAAAGGCGTAAAGAC
AGACATGAAGCAAGTGGGTTTGCAAGGAGACCAGATCCAGATTCTGATGAAGATGAAGAT
TATGAGCGAGAGAGGAGGAAAAGAAGTATGGGCGGAGCTGCCATTGCCCCACCCACTTCT
CTGGTAGAGAAAGACAAAGAGTTACCCCGAGATTTTCCTTATGAAGAGGACTCAAGACCT
CGATCACAGTCTTCCAAAGCAGCCATTCCTCCCCCAGTGTACGAGGAACAAGACAGACCG
AGATCTCCAACCGGACCTAGCAACTCCTTCCTCGCTAACATGGGGGGCACGGTGGCGCAC
AAGATCATGCAGAAGTACGGCTTCCGGGAGGGCCAGGGTCTGGGGAAGCATGAGCAGGGC
CTGAGCACTGCCTTGTCAGTGGAGAAGACCAGCAAGCGTGGCGGCAAGATCATCGTGGGC
GACGCCACAGAGAAAGATGCATCCAAGAAGTCAGATTCAAATCCGCTGACTGAAATACTT
AAGTGTCCTACTAAAGTGGTCTTACTAAGGAACATGGTTGGTGCGGGAGAGGTGGATGAA
GACTTGGAAGTTGAAACCAAGGAAGAATGTGAAAAATATGGCAAAGTTGGAAAATGTGTG
ATATTTGAAATTCCTGGTGCCCCTGATGATGAAGCAGTACGGATATTTTTAGAATTTGAG
AGAGTTGAATCAGCAATTAAAGCGGTTGTTGACTTGAATGGGAGGTATTTTGGTGGACGG
GTGGTAAAAGCATGTTTCTACAATTTGGACAAATTCAGGGTCTTGGATTTGCAGAACAAG
TT


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
>4 errors; 3 non-synonomous errors;
 insertion or deletion in coding sequence
                                

5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : RBM17
Symbol Nomenclature : RBM17
SYNONYM : SPF45
Designation : 45 kDa-splicing factor|RNA-binding motif protein 17|splicing factor 45|splicing factor 45kDa
Full Nomenclature : RNA binding motif protein 17
GENEID : 84991
Locus Tag : RP11-414H17.9
HGNC : 16944
MIM : 606935
Vega : OTTHUMG00000017618
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00440492        ATGTCCCTGTACGATGACCTAGGAGTGGAGACCAGTGACTCAAAAACAGAAGGCTGGTCC
NM_001145547.1      ATGTCCCTGTACGATGACCTAGGAGTGGAGACCAGTGACTCAAAAACAGAAGGCTGGTCC
NM_032905.4         ATGTCCCTGTACGATGACCTAGGAGTGGAGACCAGTGACTCAAAAACAGAAGGCTGGTCC
                    ************************************************************

HsCD00440492        AAAAACTTCAAACTTCTGCAGTCTCAGCTTCAGGTGAAGAAGGCAGCTCTCACTCAGGCA
NM_001145547.1      AAAAACTTCAAACTTCTGCAGTCTCAGCTTCAGGTGAAGAAGGCAGCTCTCACTCAGGCA
NM_032905.4         AAAAACTTCAAACTTCTGCAGTCTCAGCTTCAGGTGAAGAAGGCAGCTCTCACTCAGGCA
                    ************************************************************

HsCD00440492        AAGAGCCAAAGGACGAAACAAAGTACAGTCCTCGCCCCAGTCATTGACCTGAAGCGAGGT
NM_001145547.1      AAGAGCCAAAGGACGAAACAAAGTACAGTCCTCGCCCCAGTCATTGACCTGAAGCGAGGT
NM_032905.4         AAGAGCCAAAGGACGAAACAAAGTACAGTCCTCGCCCCAGTCATTGACCTGAAGCGAGGT
                    ************************************************************

HsCD00440492        GGCTCCTCAGATGACCGGCAAATTGTGGACACTCCACCGCATGTAGCAGCTGGGCTGAAG
NM_001145547.1      GGCTCCTCAGATGACCGGCAAATTGTGGACACTCCACCGCATGTAGCAGCTGGGCTGAAG
NM_032905.4         GGCTCCTCAGATGACCGGCAAATTGTGGACACTCCACCGCATGTAGCAGCTGGGCTGAAG
                    ************************************************************

HsCD00440492        GATCCTGTTCCCAGTGGGTTTTCTGCAGGGGAAGTTCTGATTCCCTTAGCTGACGAATAT
NM_001145547.1      GATCCTGTTCCCAGTGGGTTTTCTGCAGGGGAAGTTCTGATTCCCTTAGCTGACGAATAT
NM_032905.4         GATCCTGTTCCCAGTGGGTTTTCTGCAGGGGAAGTTCTGATTCCCTTAGCTGACGAATAT
                    ************************************************************

HsCD00440492        GACCCTATGTTTCCTAATGATTATGAGAAAGTAGTGAAGCGCCAAAGAGAGGAACGACAG
NM_001145547.1      GACCCTATGTTTCCTAATGATTATGAGAAAGTAGTGAAGCGCCAAAGAGAGGAACGACAG
NM_032905.4         GACCCTATGTTTCCTAATGATTATGAGAAAGTAGTGAAGCGCCAAAGAGAGGAACGACAG
                    ************************************************************

HsCD00440492        AGACAGCGGGAGCTGGAAAGACAAAAGGAAATAGAAGAAAGGGAAAAAAGGCGTAAAGAC
NM_001145547.1      AGACAGCGGGAGCTGGAAAGACAAAAGGAAATAGAAGAAAGGGAAAAAAGGCGTAAAGAC
NM_032905.4         AGACAGCGGGAGCTGGAAAGACAAAAGGAAATAGAAGAAAGGGAAAAAAGGCGTAAAGAC
                    ************************************************************

HsCD00440492        AGACATGAAGCAAGTGGGTTTGCAAGGAGACCAGATCCAGATTCTGATGAAGATGAAGAT
NM_001145547.1      AGACATGAAGCAAGTGGGTTTGCAAGGAGACCAGATCCAGATTCTGATGAAGATGAAGAT
NM_032905.4         AGACATGAAGCAAGTGGGTTTGCAAGGAGACCAGATCCAGATTCTGATGAAGATGAAGAT
                    ************************************************************

HsCD00440492        TATGAGCGAGAGAGGAGGAAAAGAAGTATGGGCGGAGCTGCCATTGCCCCACCCACTTCT
NM_001145547.1      TATGAGCGAGAGAGGAGGAAAAGAAGTATGGGCGGAGCTGCCATTGCCCCACCCACTTCT
NM_032905.4         TATGAGCGAGAGAGGAGGAAAAGAAGTATGGGCGGAGCTGCCATTGCCCCACCCACTTCT
                    ************************************************************

HsCD00440492        CTGGTAGAGAAAGACAAAGAGTTACCCCGAGATTTTCCTTATGAAGAGGACTCAAGACCT
NM_001145547.1      CTGGTAGAGAAAGACAAAGAGTTACCCCGAGATTTTCCTTATGAAGAGGACTCAAGACCT
NM_032905.4         CTGGTAGAGAAAGACAAAGAGTTACCCCGAGATTTTCCTTATGAAGAGGACTCAAGACCT
                    ************************************************************

HsCD00440492        CGATCACAGTCTTCCAAAGCAGCCATTCCTCCCCCAGTGTACGAGGAACAAGACAGACCG
NM_001145547.1      CGATCACAGTCTTCCAAAGCAGCCATTCCTCCCCCAGTGTACGAGGAACAAGACAGACCG
NM_032905.4         CGATCACAGTCTTCCAAAGCAGCCATTCCTCCCCCAGTGTACGAGGAACAAGACAGACCG
                    ************************************************************

HsCD00440492        AGATCTCCAACCGGACCTAGCAACTCCTTCCTCGCTAACATGGGGGGCACGGTGGCGCAC
NM_001145547.1      AGATCTCCAACCGGACCTAGCAACTCCTTCCTCGCTAACATGGGGGGCACGGTGGCGCAC
NM_032905.4         AGATCTCCAACCGGACCTAGCAACTCCTTCCTCGCTAACATGGGGGGCACGGTGGCGCAC
                    ************************************************************

HsCD00440492        AAGATCATGCAGAAGTACGGCTTCCGGGAGGGCCAGGGTCTGGGGAAGCATGAGCAGGGC
NM_001145547.1      AAGATCATGCAGAAGTACGGCTTCCGGGAGGGCCAGGGTCTGGGGAAGCATGAGCAGGGC
NM_032905.4         AAGATCATGCAGAAGTACGGCTTCCGGGAGGGCCAGGGTCTGGGGAAGCATGAGCAGGGC
                    ************************************************************

HsCD00440492        CTGAGCACTGCCTTGTCAGTGGAGAAGACCAGCAAGCGTGGCGGCAAGATCATCGTGGGC
NM_001145547.1      CTGAGCACTGCCTTGTCAGTGGAGAAGACCAGCAAGCGTGGCGGCAAGATCATCGTGGGC
NM_032905.4         CTGAGCACTGCCTTGTCAGTGGAGAAGACCAGCAAGCGTGGCGGCAAGATCATCGTGGGC
                    ************************************************************

HsCD00440492        GACGCCACAGAGAAAGATGCATCCAAGAAGTCAGATTCAAATCCGCTGACTGAAATACTT
NM_001145547.1      GACGCCACAGAGAAAGATGCATCCAAGAAGTCAGATTCAAATCCGCTGACTGAAATACTT
NM_032905.4         GACGCCACAGAGAAAGATGCATCCAAGAAGTCAGATTCAAATCCGCTGACTGAAATACTT
                    ************************************************************

HsCD00440492        AAGTGTCCTACTAAAGTGGTCTTACTAAGGAACATGGTTGGTGCGGGAGAGGTGGATGAA
NM_001145547.1      AAGTGTCCTACTAAAGTGGTCTTACTAAGGAACATGGTTGGTGCGGGAGAGGTGGATGAA
NM_032905.4         AAGTGTCCTACTAAAGTGGTCTTACTAAGGAACATGGTTGGTGCGGGAGAGGTGGATGAA
                    ************************************************************

HsCD00440492        GACTTGGAAGTTGAAACCAAGGAAGAATGTGAAAAATATGGCAAAGTTGGAAAATGTGTG
NM_001145547.1      GACTTGGAAGTTGAAACCAAGGAAGAATGTGAAAAATATGGCAAAGTTGGAAAATGTGTG
NM_032905.4         GACTTGGAAGTTGAAACCAAGGAAGAATGTGAAAAATATGGCAAAGTTGGAAAATGTGTG
                    ************************************************************

HsCD00440492        ATATTTGAAATTCCTGGTGCCCCTGATGATGAAGCAGTACGGATATTTTTAGAATTTGAG
NM_001145547.1      ATATTTGAAATTCCTGGTGCCCCTGATGATGAAGCAGTACGGATATTTTTAGAATTTGAG
NM_032905.4         ATATTTGAAATTCCTGGTGCCCCTGATGATGAAGCAGTACGGATATTTTTAGAATTTGAG
                    ************************************************************

HsCD00440492        AGAGTTGAATCAGCAATTAAAGCGGTTGTTGACTTGAATGGGAGGTATTTTGGTGGACGG
NM_001145547.1      AGAGTTGAATCAGCAATTAAAGCGGTTGTTGACTTGAATGGGAGGTATTTTGGTGGACGG
NM_032905.4         AGAGTTGAATCAGCAATTAAAGCGGTTGTTGACTTGAATGGGAGGTATTTTGGTGGACGG
                    ************************************************************

HsCD00440492        GTGGTAAAAGCATGTTTCTACAATTTGGACAAATTCAGGGTCTTGGATTTGCAGAACAAG
NM_001145547.1      GTGGTAAAAGCATGTTTCTACAATTTGGACAAATTCAGGGTCTTGGATTTGGCAGAACAA
NM_032905.4         GTGGTAAAAGCATGTTTCTACAATTTGGACAAATTCAGGGTCTTGGATTTGGCAGAACAA
                    *************************************************** ...*..*.

HsCD00440492        TT----
NM_001145547.1      GTTTGA
NM_032905.4         GTTTGA
                     *    

Pathways

WikiPathway : mRNA processing

Clone

Original Clone ID : ccsbBroad304_14325

Organism


TAX_ID : 9606
Species Specific ID: 84991

Genome

Chromosome : 10
Map Location : 10p15.1
Ensembl : ENSG00000134453

Reagents

Labome : SPF45-antibody