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KLHL12 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  KLHL12
Gene Name:  kelch-like family member 12
Sequence             
Original Clone ID: ccsbBroad304_08787
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00440580

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1705nts         Open reading frame : 1 to 1705
Reference Sequence Alignment

ATGGGAGGCATTATGGCCCCCAAAGACATAATGACAAATACTCATGCTAAATCCATCCTC
AATTCAATGAACTCCCTCAGGAAGAGCAATACCCTCTGTGATGTGACATTGAGAGTAGAG
CAGAAAGACTTCCCTGCCCATCGGATTGTGCTGGCTGCCTGTAGTGATTACTTCTGTGCC
ATGTTCACTAGTGAGCTCTCAGAGAAGGGGAAACCTTATGTTGACATCCAAGGTTTGACT
GCCTCTACCATGGAAATTTTATTGGACTTTGTGTACACAGAAACAGTACATGTGACAGTG
GAGAATGTACAAGAACTGCTTCCTGCAGCCTGTCTGCTTCAGTTGAAAGGTGTGAAACAA
GCCTGCTGTGAGTTCTTAGAAAGTCAGTTGGACCCTTCTAATTGCCTGGGTATTAGGGAT
TTTGCTGAAACCCACAATTGTGTTGACCTGATGCAAGCAGCTGAGGTTTTTAGCCAGAAG
CATTTTCCTGAAGTGGTACAGCATGAAGAGTTCATTCTTCTGAGTCAAGGAGAGGTGGAA
AAGCTAATCAAGTGCGACGAAATTCAGGTGGATTCTGAAGAGCCAGTCTTTGAGGCTGTC
ATCAACTGGGTGAAGCATGCCAAGAAAGAGCGGGAAGAATCCTTGCCTAACCTGCTACAG
TATGTGCGGATGCCCCTACTAACCCCCAGGTATATCACAGATGTAATAGATGCTGAGCCT
TTCATCCGCTGTAGTTTACAATGCAGGGATCTGGTTGATGAAGCAAAGAAGTTTCATCTG
AGGCCTGAACTTCGGAGTCAGATGCAGGGACCCAGGACAAGGGCTCGCCTAGGAGCCAAT
GAAGTGCTTTTGGTGGTTGGGGGCTTTGGAAGCCAGCAGTCTCCCATTGATGTGGTAGAG
AAATATGACCCCAAGACTCAGGAGTGGAGCTTTTTGCCAAGCATCACTCGTAAGAGACGT
TATGTGGCCTCAGTGTCCCTTCATGACCGGATCTACGTCATTGGTGGCTATGATGGCCGT
TCCCGCCTTAGTTCAGTGGAATGTCTAGACTACACAGCAGATGAGGATGGGGTCTGGTAT
TCTGTGGCCCCTATGAATGTCCGACGAGGTCTTGCTGGAGCCACCACCCTGGGAGATATG
ATCTATGTCTCTGGAGGCTTTGATGGAAGCAGGCGTCACACCAGTATGGAGCGCTATGAT
CCAAACATTGACCAGTGGAGCATGCTGGGAGATATGCAGACAGCCCGGGAAGGTGCCGGA
CTCGTAGTGGCCAGTGGAGTGATCTACTGTCTAGGAGGATATGACGGCTTGAATATCTTA
AATTCAGTTGAGAAATACGACCCTCATACAGGACATTGGACTAATGTTACACCAATGGCC
ACCAAGCGTTCTGGTGCAGGAGTAGCCCTGCTGAATGACCATATTTATGTGGTGGGGGGA
TTTGATGGTACAGCCCACCTTTCTTCCGTTGAAGCATACAACATTCGCACTGATTCCTGG
ACAACTGTCACCAGTATGACCACTCCACGATGCTATGTAGGGGCCACAGTGCTTCGGGGG
AGACTCTATGCAATTGCAGGATATGATGGTAATTCCCTGCTAAGTAGCATTGAATGTTAT
GACCCTATCATCGACAGCTGGGAAGTCGTGACATCCATGGGAACCCAGCGCTGTGATGCT
GGTGTTTGTGTTCTCCGCGAGAAG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : KLHL12
Symbol Nomenclature : KLHL12
SYNONYM : C3IP1; DKIR; hDKIR
Designation : CUL3-interacting protein 1|DKIR homolog|kelch-like 12|kelch-like protein 12|kelch-like protein C3IP1
Full Nomenclature : kelch-like family member 12
GENEID : 59349
GenBank Accession : EU446840
HGNC : 19360
MIM : 614522
Vega : OTTHUMG00000041385
Target GenBank: EU446840

 

Reference Sequence Alignment

  

HsCD00440580        ------------------------------------------------------------
NM_021633.2         ------------------------------------------------------------
XM_005245403.1      ATGGACGTGAATAAATTTGAGGCCTCTGTGGGTTTTCTCGATGTCAAAAAGTTCCTCAGT
                                                                                

HsCD00440580        ------------------------------------------------------ATGGGA
NM_021633.2         ------------------------------------------------------ATGGGA
XM_005245403.1      ACCTGGAAGTTGCAGAATCCAAGGACCCATTTTGTTCTTTCTCCGCACTGCTTTATGGGA
                                                                          ******

HsCD00440580        GGCATTATGGCCCCCAAAGACATAATGACAAATACTCATGCTAAATCCATCCTCAATTCA
NM_021633.2         GGCATTATGGCCCCCAAAGACATAATGACAAATACTCATGCTAAATCCATCCTCAATTCA
XM_005245403.1      GGCATTATGGCCCCCAAAGACATAATGACAAATACTCATGCTAAATCCATCCTCAATTCA
                    ************************************************************

HsCD00440580        ATGAACTCCCTCAGGAAGAGCAATACCCTCTGTGATGTGACATTGAGAGTAGAGCAGAAA
NM_021633.2         ATGAACTCCCTCAGGAAGAGCAATACCCTCTGTGATGTGACATTGAGAGTAGAGCAGAAA
XM_005245403.1      ATGAACTCCCTCAGGAAGAGCAATACCCTCTGTGATGTGACATTGAGAGTAGAGCAGAAA
                    ************************************************************

HsCD00440580        GACTTCCCTGCCCATCGGATTGTGCTGGCTGCCTGTAGTGATTACTTCTGTGCCATGTTC
NM_021633.2         GACTTCCCTGCCCATCGGATTGTGCTGGCTGCCTGTAGTGATTACTTCTGTGCCATGTTC
XM_005245403.1      GACTTCCCTGCCCATCGGATTGTGCTGGCTGCCTGTAGTGATTACTTCTGTGCCATGTTC
                    ************************************************************

HsCD00440580        ACTAGTGAGCTCTCAGAGAAGGGGAAACCTTATGTTGACATCCAAGGTTTGACTGCCTCT
NM_021633.2         ACTAGTGAGCTCTCAGAGAAGGGGAAACCTTATGTTGACATCCAAGGTTTGACTGCCTCT
XM_005245403.1      ACTAGTGAGCTCTCAGAGAAGGGGAAACCTTATGTTGACATCCAAGGTTTGACTGCCTCT
                    ************************************************************

HsCD00440580        ACCATGGAAATTTTATTGGACTTTGTGTACACAGAAACAGTACATGTGACAGTGGAGAAT
NM_021633.2         ACCATGGAAATTTTATTGGACTTTGTGTACACAGAAACGGTACATGTGACAGTGGAGAAT
XM_005245403.1      ACCATGGAAATTTTATTGGACTTTGTGTACACAGAAACAGTACATGTGACAGTGGAGAAT
                    **************************************.*********************

HsCD00440580        GTACAAGAACTGCTTCCTGCAGCCTGTCTGCTTCAGTTGAAAGGTGTGAAACAAGCCTGC
NM_021633.2         GTACAAGAACTGCTTCCTGCAGCCTGTCTGCTTCAGTTGAAAGGTGTGAAACAAGCCTGC
XM_005245403.1      GTACAAGAACTGCTTCCTGCAGCCTGTCTGCTTCAGTTGAAAGGTGTGAAACAAGCCTGC
                    ************************************************************

HsCD00440580        TGTGAGTTCTTAGAAAGTCAGTTGGACCCTTCTAATTGCCTGGGTATTAGGGATTTTGCT
NM_021633.2         TGTGAGTTCTTAGAAAGTCAGTTGGACCCTTCTAATTGCCTGGGTATTAGGGATTTTGCT
XM_005245403.1      TGTGAGTTCTTAGAAAGTCAGTTGGACCCTTCTAATTGCCTGGGTATTAGGGATTTTGCT
                    ************************************************************

HsCD00440580        GAAACCCACAATTGTGTTGACCTGATGCAAGCAGCTGAGGTTTTTAGCCAGAAGCATTTT
NM_021633.2         GAAACCCACAATTGTGTTGACCTGATGCAAGCAGCTGAGGTTTTTAGCCAGAAGCATTTT
XM_005245403.1      GAAACCCACAATTGTGTTGACCTGATGCAAGCAGCTGAGGTTTTTAGCCAGAAGCATTTT
                    ************************************************************

HsCD00440580        CCTGAAGTGGTACAGCATGAAGAGTTCATTCTTCTGAGTCAAGGAGAGGTGGAAAAGCTA
NM_021633.2         CCTGAAGTGGTACAGCATGAAGAGTTCATTCTTCTGAGTCAAGGAGAGGTGGAAAAGCTA
XM_005245403.1      CCTGAAGTGGTACAGCATGAAGAGTTCATTCTTCTGAGTCAAGGAGAGGTGGAAAAGCTA
                    ************************************************************

HsCD00440580        ATCAAGTGCGACGAAATTCAGGTGGATTCTGAAGAGCCAGTCTTTGAGGCTGTCATCAAC
NM_021633.2         ATCAAGTGCGACGAAATTCAGGTGGATTCTGAAGAGCCAGTCTTTGAGGCTGTCATCAAC
XM_005245403.1      ATCAAGTGCGACGAAATTCAGGTGGATTCTGAAGAGCCAGTCTTTGAGGCTGTCATCAAC
                    ************************************************************

HsCD00440580        TGGGTGAAGCATGCCAAGAAAGAGCGGGAAGAATCCTTGCCTAACCTGCTACAGTATGTG
NM_021633.2         TGGGTGAAGCATGCCAAGAAAGAGCGGGAAGAATCCTTGCCTAACCTGCTACAGTATGTG
XM_005245403.1      TGGGTGAAGCATGCCAAGAAAGAGCGGGAAGAATCCTTGCCTAACCTGCTACAGTATGTG
                    ************************************************************

HsCD00440580        CGGATGCCCCTACTAACCCCCAGGTATATCACAGATGTAATAGATGCTGAGCCTTTCATC
NM_021633.2         CGGATGCCCCTACTAACCCCCAGGTATATCACAGATGTAATAGATGCTGAGCCTTTCATC
XM_005245403.1      CGGATGCCCCTACTAACCCCCAGGTATATCACAGATGTAATAGATGCTGAGCCTTTCATC
                    ************************************************************

HsCD00440580        CGCTGTAGTTTACAATGCAGGGATCTGGTTGATGAAGCAAAGAAGTTTCATCTGAGGCCT
NM_021633.2         CGCTGTAGTTTACAATGCAGGGATCTGGTTGATGAAGCAAAGAAGTTTCATCTGAGGCCT
XM_005245403.1      CGCTGTAGTTTACAATGCAGGGATCTGGTTGATGAAGCAAAGAAGTTTCATCTGAGGCCT
                    ************************************************************

HsCD00440580        GAACTTCGGAGTCAGATGCAGGGACCCAGGACAAGGGCTCGCCTAGGAGCCAATGAAGTG
NM_021633.2         GAACTTCGGAGTCAGATGCAGGGACCCAGGACAAGGGCTCGCCTAGGAGCCAATGAAGTG
XM_005245403.1      GAACTTCGGAGTCAGATGCAGGGACCCAGGACAAGGGCTCGCCTAGGAGCCAATGAAGTG
                    ************************************************************

HsCD00440580        CTTTTGGTGGTTGGGGGCTTTGGAAGCCAGCAGTCTCCCATTGATGTGGTAGAGAAATAT
NM_021633.2         CTTTTGGTGGTTGGGGGCTTTGGAAGCCAGCAGTCTCCCATTGATGTGGTAGAGAAATAT
XM_005245403.1      CTTTTGGTGGTTGGGGGCTTTGGAAGCCAGCAGTCTCCCATTGATGTGGTAGAGAAATAT
                    ************************************************************

HsCD00440580        GACCCCAAGACTCAGGAGTGGAGCTTTTTGCCAAGCATCACTCGTAAGAGACGTTATGTG
NM_021633.2         GACCCCAAGACTCAGGAGTGGAGCTTTTTGCCAAGCATCACTCGTAAGAGACGTTATGTG
XM_005245403.1      GACCCCAAGACTCAGGAGTGGAGCTTTTTGCCAAGCATCACTCGTAAGAGACGTTATGTG
                    ************************************************************

HsCD00440580        GCCTCAGTGTCCCTTCATGACCGGATCTACGTCATTGGTGGCTATGATGGCCGTTCCCGC
NM_021633.2         GCCTCAGTGTCCCTTCATGACCGGATCTACGTCATTGGTGGCTATGATGGCCGTTCCCGC
XM_005245403.1      GCCTCAGTGTCCCTTCATGACCGGATCTACGTCATTGGTGGCTATGATGGCCGTTCCCGC
                    ************************************************************

HsCD00440580        CTTAGTTCAGTGGAATGTCTAGACTACACAGCAGATGAGGATGGGGTCTGGTATTCTGTG
NM_021633.2         CTTAGTTCAGTGGAATGTCTAGACTACACAGCAGATGAGGATGGGGTCTGGTATTCTGTG
XM_005245403.1      CTTAGTTCAGTGGAATGTCTAGACTACACAGCAGATGAGGATGGGGTCTGGTATTCTGTG
                    ************************************************************

HsCD00440580        GCCCCTATGAATGTCCGACGAGGTCTTGCTGGAGCCACCACCCTGGGAGATATGATCTAT
NM_021633.2         GCCCCTATGAATGTCCGACGAGGTCTTGCTGGAGCCACCACCCTGGGAGATATGATCTAT
XM_005245403.1      GCCCCTATGAATGTCCGACGAGGTCTTGCTGGAGCCACCACCCTGGGAGATATGATCTAT
                    ************************************************************

HsCD00440580        GTCTCTGGAGGCTTTGATGGAAGCAGGCGTCACACCAGTATGGAGCGCTATGATCCAAAC
NM_021633.2         GTCTCTGGAGGCTTTGATGGAAGCAGGCGTCACACCAGTATGGAGCGCTATGATCCAAAC
XM_005245403.1      GTCTCTGGAGGCTTTGATGGAAGCAGGCGTCACACCAGTATGGAGCGCTATGATCCAAAC
                    ************************************************************

HsCD00440580        ATTGACCAGTGGAGCATGCTGGGAGATATGCAGACAGCCCGGGAAGGTGCCGGACTCGTA
NM_021633.2         ATTGACCAGTGGAGCATGCTGGGGGATATGCAGACAGCCCGGGAAGGTGCCGGACTCGTA
XM_005245403.1      ATTGACCAGTGGAGCATGCTGGGAGATATGCAGACAGCCCGGGAAGGTGCCGGACTCGTA
                    ***********************.************************************

HsCD00440580        GTGGCCAGTGGAGTGATCTACTGTCTAGGAGGATATGACGGCTTGAATATCTTAAATTCA
NM_021633.2         GTGGCCAGTGGAGTGATCTACTGTCTAGGAGGATATGACGGCTTGAATATCTTAAATTCA
XM_005245403.1      GTGGCCAGTGGAGTGATCTACTGTCTAGGAGGATATGACGGCTTGAATATCTTAAATTCA
                    ************************************************************

HsCD00440580        GTTGAGAAATACGACCCTCATACAGGACATTGGACTAATGTTACACCAATGGCCACCAAG
NM_021633.2         GTTGAGAAATACGACCCTCATACAGGACATTGGACTAATGTTACACCAATGGCCACCAAG
XM_005245403.1      GTTGAGAAATACGACCCTCATACAGGACATTGGACTAATGTTACACCAATGGCCACCAAG
                    ************************************************************

HsCD00440580        CGTTCTGGTGCAGGAGTAGCCCTGCTGAATGACCATATTTATGTGGTGGGGGGATTTGAT
NM_021633.2         CGTTCTGGTGCAGGAGTAGCCCTGCTGAATGACCATATTTATGTGGTGGGGGGATTTGAT
XM_005245403.1      CGTTCTGGTGCAGGAGTAGCCCTGCTGAATGACCATATTTATGTGGTGGGGGGATTTGAT
                    ************************************************************

HsCD00440580        GGTACAGCCCACCTTTCTTCCGTTGAAGCATACAACATTCGCACTGATTCCTGGACAACT
NM_021633.2         GGTACAGCCCACCTTTCTTCCGTTGAAGCATACAACATTCGCACTGATTCCTGGACAACT
XM_005245403.1      GGTACAGCCCACCTTTCTTCCGTTGAAGCATACAACATTCGCACTGATTCCTGGACAACT
                    ************************************************************

HsCD00440580        GTCACCAGTATGACCACTCCACGATGCTATGTAGGGGCCACAGTGCTTCGGGGGAGACTC
NM_021633.2         GTCACCAGTATGACCACTCCACGATGCTATGTAGGGGCCACAGTGCTTCGGGGGAGACTC
XM_005245403.1      GTCACCAGTATGACCACTCCACGATGCTATGTAGGGGCCACAGTGCTTCGGGGGAGACTC
                    ************************************************************

HsCD00440580        TATGCAATTGCAGGATATGATGGTAATTCCCTGCTAAGTAGCATTGAATGTTATGACCCT
NM_021633.2         TATGCAATTGCAGGATATGATGGTAATTCCCTGCTAAGTAGCATTGAATGTTATGACCCT
XM_005245403.1      TATGCAATTGCAGGATATGATGGTAATTCCCTGCTAAGTAGCATTGAATGTTATGACCCT
                    ************************************************************

HsCD00440580        ATCATCGACAGCTGGGAAGTCGTGACATCCATGGGAACCCAGCGCTGTGATGCTGGTGTT
NM_021633.2         ATCATCGACAGCTGGGAAGTCGTGACATCCATGGGAACCCAGCGCTGTGATGCTGGTGTT
XM_005245403.1      ATCATCGACAGCTGGGAAGTCGTGACATCCATGGGAACCCAGCGCTGTGATGCTGGTGTT
                    ************************************************************

HsCD00440580        TGTGTTCTCCGCGAGAAG---
NM_021633.2         TGTGTTCTCCGCGAGAAGTGA
XM_005245403.1      TGTGTTCTCCGCGAGAAGTGA
                    ******************   

Pathways

NCI : Canonical Wnt signaling pathway
Reactome : Signal Transduction
Reactome : Signaling by Wnt
Reactome : TCF dependent signaling in response to WNT
Reactome : degradation of DVL

Protein

SMART domain : BACK : BTB And C-terminal Kelch
SMART domain : BTB : Broad-Complex, Tramtrack and Bric a brac
SMART domain : Kelch :
HPRD : 13925

Clone

Original Clone ID : ccsbBroad304_08787

Organism


TAX_ID : 9606
Species Specific ID: 59349

Genome

Chromosome : 1
Map Location : 1q32.1
Ensembl : ENSG00000117153

Reagents

Labome : KLHL12-antibody