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NPM1 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  NPM1
Gene Name:  nucleophosmin (nucleolar phosphoprotein B23, numatrin)
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH110575.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 16512675
Title: Functional proteomics approach to investigate the biological activities of cDNAs implicated in breast cancer.
Authors: HIP
HsCD00004411

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 885nts         Open reading frame : 1 to 885
Reference Sequence Alignment

ATGGAAGATTCGATGGACATGGACATGAGCCCCCTGAGGCCCCAGAACTATCTTTTCGGT
TGTGAACTAAAGGCCGACAAAGATTATCACTTTAAGGTGGATAATGATGAAAATGAGCAC
CAGTTATCTTTAAGAACGGTCAGTTTAGGGGCTGGTGCAAAGGATGAGTTGCACATTGTT
GAAGCAGAGGCAATGAATTACGAAGGCAGTCCAATTAAAGTAACACTGGCAACTTTGAAA
ATGTCTGTACAGCCAACGGTTTCCCTTGGGGGCTTTGAAATAACACCACCAGTGGTCTTA
AGGTTGAAGTGTGGTTCAGGGCCAGTGCATATTAGTGGACAGCACTTAGTAGCTGTGGAG
GAAGATGCAGAGTCAGAAGATGAAGAGGAGGAGGATGTGAAACTCTTAAGTATATCTGGA
AAGCGGTCTGCCCCTGGAGGTGGTAGCAAGGTTCCACAGAAAAAAGTAAAACTTGCTGCT
GATGAAGATGATGACGATGATGATGAAGAGGATGATGATGAAGATGATGATGATGATGAT
TTTGATGATGAGGAAGCTGAAGAAAAAGCGCCAGTGAAGAAATCTATACGAGATACTCCA
GCCAAAAATGCACAAAAGTCAAATCAGAATGGAAAAGACTCAAAACCATCATCAACACCA
AGATCAAAAGGACAAGAATCCTTCAAGAAACAGGAAAAAACTCCTAAAACACCAAAAGGA
CCTAGTTCTGTAGAAGACATTAAAGCAAAAATGCAAGCAAGTATAGAAAAAGGTGGTTCT
CTTCCCAAAGTGGAAGCCAAATTCATCAATTATGTGAAGAATTGCTTCCGGATGACTGAC
CAAGAGGCTATTCAAGATCTCTGGCAGTGGAGGAAGTCTCTTTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 982
Start on reference sequence 98
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : NPM1
Symbol Nomenclature : NPM1
SYNONYM : B23; NPM
Designation : nucleolar protein NO38|nucleophosmin|nucleophosmin/nucleoplasmin family, member 1
Full Nomenclature : nucleophosmin (nucleolar phosphoprotein B23, numatrin)
GENEID : 4869
GI : 61361628
GenBank Accession : AY890136
HGNC : 7910
MIM : 164040
Vega : OTTHUMG00000130465
Target GenBank: BC008495

 

Reference Sequence Alignment

  

HsCD00004411      ATGGAAGATTCGATGGACATGGACATGAGCCCCCTGAGGCCCCAGAACTATCTTTTCGGT
NM_002520.6       ATGGAAGATTCGATGGACATGGACATGAGCCCCCTGAGGCCCCAGAACTATCTTTTCGGT
                  ************************************************************

HsCD00004411      TGTGAACTAAAGGCCGACAAAGATTATCACTTTAAGGTGGATAATGATGAAAATGAGCAC
NM_002520.6       TGTGAACTAAAGGCCGACAAAGATTATCACTTTAAGGTGGATAATGATGAAAATGAGCAC
                  ************************************************************

HsCD00004411      CAGTTATCTTTAAGAACGGTCAGTTTAGGGGCTGGTGCAAAGGATGAGTTGCACATTGTT
NM_002520.6       CAGTTATCTTTAAGAACGGTCAGTTTAGGGGCTGGTGCAAAGGATGAGTTGCACATTGTT
                  ************************************************************

HsCD00004411      GAAGCAGAGGCAATGAATTACGAAGGCAGTCCAATTAAAGTAACACTGGCAACTTTGAAA
NM_002520.6       GAAGCAGAGGCAATGAATTACGAAGGCAGTCCAATTAAAGTAACACTGGCAACTTTGAAA
                  ************************************************************

HsCD00004411      ATGTCTGTACAGCCAACGGTTTCCCTTGGGGGCTTTGAAATAACACCACCAGTGGTCTTA
NM_002520.6       ATGTCTGTACAGCCAACGGTTTCCCTTGGGGGCTTTGAAATAACACCACCAGTGGTCTTA
                  ************************************************************

HsCD00004411      AGGTTGAAGTGTGGTTCAGGGCCAGTGCATATTAGTGGACAGCACTTAGTAGCTGTGGAG
NM_002520.6       AGGTTGAAGTGTGGTTCAGGGCCAGTGCATATTAGTGGACAGCACTTAGTAGCTGTGGAG
                  ************************************************************

HsCD00004411      GAAGATGCAGAGTCAGAAGATGAAGAGGAGGAGGATGTGAAACTCTTAAGTATATCTGGA
NM_002520.6       GAAGATGCAGAGTCAGAAGATGAAGAGGAGGAGGATGTGAAACTCTTAAGTATATCTGGA
                  ************************************************************

HsCD00004411      AAGCGGTCTGCCCCTGGAGGTGGTAGCAAGGTTCCACAGAAAAAAGTAAAACTTGCTGCT
NM_002520.6       AAGCGGTCTGCCCCTGGAGGTGGTAGCAAGGTTCCACAGAAAAAAGTAAAACTTGCTGCT
                  ************************************************************

HsCD00004411      GATGAAGATGATGACGATGATGATGAAGAGGATGATGATGAAGATGATGATGATGATGAT
NM_002520.6       GATGAAGATGATGACGATGATGATGAAGAGGATGATGATGAAGATGATGATGATGATGAT
                  ************************************************************

HsCD00004411      TTTGATGATGAGGAAGCTGAAGAAAAAGCGCCAGTGAAGAAATCTATACGAGATACTCCA
NM_002520.6       TTTGATGATGAGGAAGCTGAAGAAAAAGCGCCAGTGAAGAAATCTATACGAGATACTCCA
                  ************************************************************

HsCD00004411      GCCAAAAATGCACAAAAGTCAAATCAGAATGGAAAAGACTCAAAACCATCATCAACACCA
NM_002520.6       GCCAAAAATGCACAAAAGTCAAATCAGAATGGAAAAGACTCAAAACCATCATCAACACCA
                  ************************************************************

HsCD00004411      AGATCAAAAGGACAAGAATCCTTCAAGAAACAGGAAAAAACTCCTAAAACACCAAAAGGA
NM_002520.6       AGATCAAAAGGACAAGAATCCTTCAAGAAACAGGAAAAAACTCCTAAAACACCAAAAGGA
                  ************************************************************

HsCD00004411      CCTAGTTCTGTAGAAGACATTAAAGCAAAAATGCAAGCAAGTATAGAAAAAGGTGGTTCT
NM_002520.6       CCTAGTTCTGTAGAAGACATTAAAGCAAAAATGCAAGCAAGTATAGAAAAAGGTGGTTCT
                  ************************************************************

HsCD00004411      CTTCCCAAAGTGGAAGCCAAATTCATCAATTATGTGAAGAATTGCTTCCGGATGACTGAC
NM_002520.6       CTTCCCAAAGTGGAAGCCAAATTCATCAATTATGTGAAGAATTGCTTCCGGATGACTGAC
                  ************************************************************

HsCD00004411      CAAGAGGCTATTCAAGATCTCTGGCAGTGGAGGAAGTCTCTTTAG
NM_002520.6       CAAGAGGCTATTCAAGATCTCTGGCAGTGGAGGAAGTCTCTTTAA
                  ********************************************.

Pathways

NCI : Aurora B signaling
NCI : BARD1 signaling events
NCI : HIF-1-alpha transcription factor network
NCI : Validated targets of C-MYC transcriptional activation
Reactome : Cell Cycle
Reactome : Chromosome Maintenance
Reactome : Deposition of new CENPA-containing nucleosomes at the centromere
Reactome : Disease
Reactome : HIV Infection
Reactome : Host Interactions of HIV factors
Reactome : Interactions of Rev with host cellular proteins
Reactome : Nuclear import of Rev protein
Reactome : Nucleosome assembly

Protein

UniProt : Q9BYG9
UniProt : P06748
HPRD : 01246

Clone

Cloning Information : 110575
HIP Master Clone ID : 87002
Original Clone ID : FLH110575.01X

Organism


TAX_ID : 9606
Species Specific ID: 4869

Genome

Chromosome : 5
Map Location : 5q35.1
Ensembl : ENSG00000181163

Reagents

Labome : NPM1-antibody