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RGR (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  RGR
Gene Name:  retinal G protein coupled receptor
Sequence             
Original Clone ID: ccsbBroad304_06860
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00441141

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 886nts         Open reading frame : 1 to 886
Reference Sequence Alignment

ATGGCAGAGACCAGTGCCTTGCCCACCGGCTTCGGGGAGCTCGAGGTGCTGGCTGTGGGG
ATGGTGCTACTGGTGGAAGCTCTCTCCGGTCTCAGCCTCAATACCCTGACCATCTTCTCT
TTCTGCAAGACCCCGGAGCTGCGGACTCCCTGCCACCTACTGGTGCTGAGCTTGGCTCTT
GCGGACAGTGGGATCAGCCTGAATGCCCTCGTTGCAGCCACATCCAGCCTTCTCCGTGTC
TCCCACAGGCGCTGGCCCTACGGCTCGGACGGCTGCCAGGCTCACGGCTTCCAGGGCTTT
GTGACAGCGTTGGCCAGCATCTGCAGCAGTGCAGCCATCGCATGGGGGCGTTATCACCAC
TACTGCACCCGTAGCCAGCTGGCCTGGAACTCAGCCGTCTCTCTGGTGCTCTTCGTGTGG
CTGTCTTCTGCCTTCTGGGCAGCTCTGCCCCTTCTGGGTTGGGGTCACTATGACTATGAG
CCACTGGGGACATGCTGCACCCTGGACTACTCCAAGGGGGACAGAAACTTCACCAGCTTC
CTCTTCACCATGTCCTTCTTCAACTTCGCCATGCCCCTCTTCATCACGATCACTTCCTAC
AGTCTCATGGAGCAGAAACTGGGGAAGAGTGGCCATCTCCAGGTAAACACCACTCTGCCA
GCAAGGACGCTGCTGCTCGGCTGGGGCCCCTATGCCATCCTGTATCTATACGCAGTCATC
GCAGACGTGACTTCCATCTCCCCCAAACTGCAGATGGTGCCCGCCCTCATTGCCAAAATG
GTGCCCACGATCAATGCCATCAACTATGCCCTGGGCAATGAGATGGTCTGCAGGGGAATC
TGGCAGTGCCTCTCACCGCAGAAGAGGGAGAAGGACCGAACCAAG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : RGR
Symbol Nomenclature : RGR
SYNONYM : RP44
Designation : RGR-opsin|RPE retinal G-protein coupled receptor|RPE-retinal G protein-coupled receptor
Full Nomenclature : retinal G protein coupled receptor
GENEID : 5995
GenBank Accession : DQ895267
HGNC : 9990
MIM : 600342
Target GenBank: DQ895267

 

Reference Sequence Alignment

  

HsCD00441141        ATGGCAGAGACCAGTGCCTTGCCCACCGGCTTCGGGGAGCTCGAGGTGCTGGCTGTGGGG
NM_001012720.1      ATGGCAGAGACCAGTGCCCTGCCCACTGGCTTCGGGGAGCTCGAGGTGCTGGCTGTGGGG
NM_002921.3         ATGGCAGAGACCAGTGCCCTGCCCACTGGCTTCGGGGAGCTCGAGGTGCTGGCTGTGGGG
                    ****************** ******* *********************************

HsCD00441141        ATGGTGCTACTGGTGGAAGCTCTCTCCGGTCTCAGCCTCAATACCCTGACCATCTTCTCT
NM_001012720.1      ATGGTGCTACTGGTGGAAGCTCTCTCCGGTCTCAGCCTCAATACCCTGACCATCTTCTCT
NM_002921.3         ATGGTGCTACTGGTGGAAGCTCTCTCCGGTCTCAGCCTCAATACCCTGACCATCTTCTCT
                    ************************************************************

HsCD00441141        TTCTGCAAGACCCCGGAGCTGCGGACTCCCTGCCACCTACTGGTGCTGAGCTTGGCTCTT
NM_001012720.1      TTCTGCAAGACCCCGGAGCTGCGGACTCCCTGCCACCTACTGGTGCTGAGCTTGGCTCTT
NM_002921.3         TTCTGCAAGACCCCGGAGCTGCGGACTCCCTGCCACCTACTGGTGCTGAGCTTGGCTCTT
                    ************************************************************

HsCD00441141        GCGGACAGTGGGATCAGCCTGAATGCCCTCGTTGCAGCCACATCCAGCCTTCTCCGTGTC
NM_001012720.1      GCGGACAGTGGGATCAGCCTGAATGCCCTCGTTGCAGCCACATCCAGCCTTCTCCGG---
NM_002921.3         GCGGACAGTGGGATCAGCCTGAATGCCCTCGTTGCAGCCACATCCAGCCTTCTCCGTGTC
                    ********************************************************    

HsCD00441141        TCCCACAGGCGCTGGCCCTACGGCTCGGACGGCTGCCAGGCTCACGGCTTCCAGGGCTTT
NM_001012720.1      ---------CGCTGGCCCTACGGCTCGGACGGCTGCCAGGCTCACGGCTTCCAGGGCTTT
NM_002921.3         TCCCACAGGCGCTGGCCCTACGGCTCGGACGGCTGCCAGGCTCACGGCTTCCAGGGCTTT
                             ***************************************************

HsCD00441141        GTGACAGCGTTGGCCAGCATCTGCAGCAGTGCAGCCATCGCATGGGGGCGTTATCACCAC
NM_001012720.1      GTGACAGCGTTGGCCAGCATCTGCAGCAGTGCAGCCATCGCATGGGGGCGTTATCACCAC
NM_002921.3         GTGACAGCGTTGGCCAGCATCTGCAGCAGTGCAGCCATCGCATGGGGGCGTTATCACCAC
                    ************************************************************

HsCD00441141        TACTGCACCCGTAGCCAGCTGGCCTGGAACTCAGCCGTCTCTCTGGTGCTCTTCGTGTGG
NM_001012720.1      TACTGCACCCGTAGCCAGCTGGCCTGGAACTCAGCCGTCTCTCTGGTGCTCTTCGTGTGG
NM_002921.3         TACTGCACCCGTAGCCAGCTGGCCTGGAACTCAGCCGTCTCTCTGGTGCTCTTCGTGTGG
                    ************************************************************

HsCD00441141        CTGTCTTCTGCCTTCTGGGCAGCTCTGCCCCTTCTGGGTTGGGGTCACTATGACTATGAG
NM_001012720.1      CTGTCTTCTGCCTTCTGGGCAGCTCTGCCCCTTCTGGGTTGGGGTCACTACGACTATGAG
NM_002921.3         CTGTCTTCTGCCTTCTGGGCAGCTCTGCCCCTTCTGGGTTGGGGTCACTACGACTATGAG
                    ************************************************** *********

HsCD00441141        CCACTGGGGACATGCTGCACCCTGGACTACTCCAAGGGGGACAGAAACTTCACCAGCTTC
NM_001012720.1      CCACTGGGGACATGCTGCACCCTGGACTACTCCAAGGGGGACAGAAACTTCACCAGCTTC
NM_002921.3         CCACTGGGGACATGCTGCACCCTGGACTACTCCAAGGGGGACAGAAACTTCACCAGCTTC
                    ************************************************************

HsCD00441141        CTCTTCACCATGTCCTTCTTCAACTTCGCCATGCCCCTCTTCATCACGATCACTTCCTAC
NM_001012720.1      CTCTTCACCATGTCCTTCTTCAACTTCGCCATGCCCCTCTTCATCACGATCACTTCCTAC
NM_002921.3         CTCTTCACCATGTCCTTCTTCAACTTCGCCATGCCCCTCTTCATCACGATCACTTCCTAC
                    ************************************************************

HsCD00441141        AGTCTCATGGAGCAGAAACTGGGGAAGAGTGGCCATCTCCAGGTAAACACCACTCTGCCA
NM_001012720.1      AGTCTCATGGAGCAGAAACTGGGGAAGAGTGGCCATCTCCAGGTAAACACCACTCTGCCA
NM_002921.3         AGTCTCATGGAGCAGAAACTGGGGAAGAGTGGCCATCTCCAGGTAAACACCACTCTGCCA
                    ************************************************************

HsCD00441141        GCAAGGACGCTGCTGCTCGGCTGGGGCCCCTATGCCATCCTGTATCTATACGCAGTCATC
NM_001012720.1      GCAAGGACGCTGCTGCTCGGCTGGGGCCCCTATGCCATCCTGTATCTATACGCAGTCATC
NM_002921.3         GCAAGGACGCTGCTGCTCGGCTGGGGCCCCTATGCCATCCTGTATCTATACGCAGTCATC
                    ************************************************************

HsCD00441141        GCAGACGTGACTTCCATCTCCCCCAAACTGCAGATGGTGCCCGCCCTCATTGCCAAAATG
NM_001012720.1      GCAGACGTGACTTCCATCTCCCCCAAACTGCAGATGGTGCCCGCCCTCATTGCCAAAATG
NM_002921.3         GCAGACGTGACTTCCATCTCCCCCAAACTGCAGATGGTGCCCGCCCTCATTGCCAAAATG
                    ************************************************************

HsCD00441141        GTGCCCACGATCAATGCCATCAACTATGCCCTGGGCAATGAGATGGTCTGCAGGGGAATC
NM_001012720.1      GTGCCCACGATCAATGCCATCAACTATGCCCTGGGCAATGAGATGGTCTGCAGGGGAATC
NM_002921.3         GTGCCCACGATCAATGCCATCAACTATGCCCTGGGCAATGAGATGGTCTGCAGGGGAATC
                    ************************************************************

HsCD00441141        TGGCAGTGCCTCTCACCGCAGAAGAGGGAGAAGGACCGAACCAAG---
NM_001012720.1      TGGCAGTGCCTCTCACCGCAGAAGAGGGAGAAGGACCGAACCAAGTGA
NM_002921.3         TGGCAGTGCCTCTCACCGCAGAAGAGGGAGAAGGACCGAACCAAGTGA
                    *********************************************   

Pathways

Reactome : Class A/1 (Rhodopsin-like receptors)
Reactome : G alpha (i) signalling events
Reactome : GPCR downstream signaling
Reactome : GPCR ligand binding
Reactome : Opsins
Reactome : Signal Transduction
Reactome : Signaling by GPCR

Protein

HPRD : 02642

Clone

Original Clone ID : ccsbBroad304_06860

Organism


TAX_ID : 9606
Species Specific ID: 5995

Genome

Chromosome : 10
Map Location : 10q23

Reagents

Labome : RGR-antibody