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LGALS8 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  LGALS8
Gene Name:  lectin, galactoside-binding, soluble, 8
Sequence             
Original Clone ID: ccsbBroad304_10205
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00441250

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 949nts         Open reading frame : 1 to 949
Reference Sequence Alignment

ATGTTGTCCTTAAACAACCTACAGAATATCATCTATAACCCGGTAATCCCGTTTGTTGGC
ACCATTCCTGATCAGCTGGATCCTGGAACTTTGATTGTGATACGTGGGCATGTTCCTAGT
GACGCAGACAGATTCCAGGTGGATCTGCAGAATGGCAGCAGCATGAAACCTCGAGCCGAT
GTGGCCTTTCATTTCAATCCTCGTTTCAAAAGGGCCGGCTGCATTGTTTGCAATACTTTG
ATAAATGAAAAATGGGGACGGGAAGAGATCACCTATGACACGCCTTTCAAAAGAGAAAAG
TCTTTTGAGATCGTGATTATGGTGCTGAAGGACAAATTCCAGGTGGCTGTAAATGGAAAA
CATACTCTGCTCTATGGCCACAGGATCGGCCCAGAGAAAATAGACACTCTGGGCATTTAT
GGCAAAGTGAATATTCACTCAATTGGTTTTAGCTTCAGCTCGGACTTACAAAGTACCCAA
GCATCTAGTCTGGAACTGACAGAGATAAGTAGAGAAAATGTTCCAAAGTCTGGCACGCCC
CAGCTTAGGCTGCCATTCGCTGCAAGGTTGAACACCCCCATGGGCCCTGGACGAACTGTC
GTCGTTAAAGGAGAAGTGAATGCAAATGCCAAAAGCTTTAATGTTGACCTACTAGCAGGA
AAATCAAAGGATATTGCTCTACACTTGAACCCACGCCTGAATATTAAAGCATTTGTAAGA
AATTCTTTTCTTCAGGAGTCCTGGGGAGAAGAAGAGAGAAATATTACCTCTTTCCCATTT
AGTCCTGGGATGTACTTTGAGATGATAATTTATTGTGATGTTAGAGAATTCAAGGTTGCA
GTAAATGGCGTACACAGCCTGGAGTACAAACACAGATTTAAAGAGCTCAGCAGTATTGAC
ACGCTGGAAATTAATGGAGACATCCACTTACTGGAAGTAAGGAGCTGG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : LGALS8
Symbol Nomenclature : LGALS8
SYNONYM : Gal-8; PCTA-1; PCTA1; Po66-CBP
Designation : Po66 carbohydrate binding protein|galectin 8|galectin-8|galectin-8g|po66 carbohydrate-binding protein|prostate carcinoma tumor antigen 1
Full Nomenclature : lectin, galactoside-binding, soluble, 8
GENEID : 3964
Locus Tag : RP11-385F5.1
GenBank Accession : EU176401
HGNC : 6569
MIM : 606099
Vega : OTTHUMG00000039953
Target GenBank: EU176401

 

Reference Sequence Alignment

  

HsCD00441250      ---ATGTTGTCCTTAAACAACCTACAGAATATCATCTATAACCCGGTAATCCCGTTTGTT
NM_201544.2       ATGATGTTGTCCTTAAACAACCTACAGAATATCATCTATAACCCGGTAATCCCGTTTGTT
NM_201543.2       ATGATGTTGTCCTTAAACAACCTACAGAATATCATCTATAACCCGGTAATCCCGTTTGTT
                     *********************************************************

HsCD00441250      GGCACCATTCCTGATCAGCTGGATCCTGGAACTTTGATTGTGATACGTGGGCATGTTCCT
NM_201544.2       GGCACCATTCCTGATCAGCTGGATCCTGGAACTTTGATTGTGATACGTGGGCATGTTCCT
NM_201543.2       GGCACCATTCCTGATCAGCTGGATCCTGGAACTTTGATTGTGATACGTGGGCATGTTCCT
                  ************************************************************

HsCD00441250      AGTGACGCAGACAGATTCCAGGTGGATCTGCAGAATGGCAGCAGCATGAAACCTCGAGCC
NM_201544.2       AGTGACGCAGACAGATTCCAGGTGGATCTGCAGAATGGCAGCAGCATGAAACCTCGAGCC
NM_201543.2       AGTGACGCAGACAGATTCCAGGTGGATCTGCAGAATGGCAGCAGCATGAAACCTCGAGCC
                  ************************************************************

HsCD00441250      GATGTGGCCTTTCATTTCAATCCTCGTTTCAAAAGGGCCGGCTGCATTGTTTGCAATACT
NM_201544.2       GATGTGGCCTTTCATTTCAATCCTCGTTTCAAAAGGGCCGGCTGCATTGTTTGCAATACT
NM_201543.2       GATGTGGCCTTTCATTTCAATCCTCGTTTCAAAAGGGCCGGCTGCATTGTTTGCAATACT
                  ************************************************************

HsCD00441250      TTGATAAATGAAAAATGGGGACGGGAAGAGATCACCTATGACACGCCTTTCAAAAGAGAA
NM_201544.2       TTGATAAATGAAAAATGGGGACGGGAAGAGATCACCTATGACACGCCTTTCAAAAGAGAA
NM_201543.2       TTGATAAATGAAAAATGGGGACGGGAAGAGATCACCTATGACACGCCTTTCAAAAGAGAA
                  ************************************************************

HsCD00441250      AAGTCTTTTGAGATCGTGATTATGGTGCTGAAGGACAAATTCCAGGTGGCTGTAAATGGA
NM_201544.2       AAGTCTTTTGAGATCGTGATTATGGTGCTGAAGGACAAATTCCAGGTGGCTGTAAATGGA
NM_201543.2       AAGTCTTTTGAGATCGTGATTATGGTGCTGAAGGACAAATTCCAGGTGGCTGTAAATGGA
                  ************************************************************

HsCD00441250      AAACATACTCTGCTCTATGGCCACAGGATCGGCCCAGAGAAAATAGACACTCTGGGCATT
NM_201544.2       AAACATACTCTGCTCTATGGCCACAGGATCGGCCCAGAGAAAATAGACACTCTGGGCATT
NM_201543.2       AAACATACTCTGCTCTATGGCCACAGGATCGGCCCAGAGAAAATAGACACTCTGGGCATT
                  ************************************************************

HsCD00441250      TATGGCAAAGTGAATATTCACTCAATTGGTTTTAGCTTCAGCTCGGACTTACAAAGTACC
NM_201544.2       TATGGCAAAGTGAATATTCACTCAATTGGTTTTAGCTTCAGCTCGGACTTACAAAGTACC
NM_201543.2       TATGGCAAAGTGAATATTCACTCAATTGGTTTTAGCTTCAGCTCGGACTTACAAAGTACC
                  ************************************************************

HsCD00441250      CAAGCATCTAGTCTGGAACTGACAGAGATAAGTAGAGAAAATGTTCCAAAGTCTGGCACG
NM_201544.2       CAAGCATCTAGTCTGGAACTGACAGAGATAAGTAGAGAAAATGTTCCAAAGTCTGGCACG
NM_201543.2       CAAGCATCTAGTCTGGAACTGACAGAGATAAGTAGAGAAAATGTTCCAAAGTCTGGCACG
                  ************************************************************

HsCD00441250      CCCCAGCTTAGGCTGCCATTCGCTGCAAGGTTGAACACCCCCATGGGCCCTGGACGAACT
NM_201544.2       CCCCAGCTTAGGCTGCCATTCGCTGCAAGGTTGAACACCCCCATGGGCCCTGGACGAACT
NM_201543.2       CCCCAGCTTAGGCTGCCATTCGCTGCAAGGTTGAACACCCCCATGGGCCCTGGACGAACT
                  ************************************************************

HsCD00441250      GTCGTCGTTAAAGGAGAAGTGAATGCAAATGCCAAAAGCTTTAATGTTGACCTACTAGCA
NM_201544.2       GTCGTCGTTAAAGGAGAAGTGAATGCAAATGCCAAAAGCTTTAATGTTGACCTACTAGCA
NM_201543.2       GTCGTCGTTAAAGGAGAAGTGAATGCAAATGCCAAAAGCTTTAATGTTGACCTACTAGCA
                  ************************************************************

HsCD00441250      GGAAAATCAAAGGATATTGCTCTACACTTGAACCCACGCCTGAATATTAAAGCATTTGTA
NM_201544.2       GGAAAATCAAAGGATATTGCTCTACACTTGAACCCACGCCTGAATATTAAAGCATTTGTA
NM_201543.2       GGAAAATCAAAGGATATTGCTCTACACTTGAACCCACGCCTGAATATTAAAGCATTTGTA
                  ************************************************************

HsCD00441250      AGAAATTCTTTTCTTCAGGAGTCCTGGGGAGAAGAAGAGAGAAATATTACCTCTTTCCCA
NM_201544.2       AGAAATTCTTTTCTTCAGGAGTCCTGGGGAGAAGAAGAGAGAAATATTACCTCTTTCCCA
NM_201543.2       AGAAATTCTTTTCTTCAGGAGTCCTGGGGAGAAGAAGAGAGAAATATTACCTCTTTCCCA
                  ************************************************************

HsCD00441250      TTTAGTCCTGGGATGTACTTTGAGATGATAATTTATTGTGATGTTAGAGAATTCAAGGTT
NM_201544.2       TTTAGTCCTGGGATGTACTTTGAGATGATAATTTATTGTGATGTTAGAGAATTCAAGGTT
NM_201543.2       TTTAGTCCTGGGATGTACTTTGAGATGATAATTTATTGTGATGTTAGAGAATTCAAGGTT
                  ************************************************************

HsCD00441250      GCAGTAAATGGCGTACACAGCCTGGAGTACAAACACAGATTTAAAGAGCTCAGCAGTATT
NM_201544.2       GCAGTAAATGGCGTACACAGCCTGGAGTACAAACACAGATTTAAAGAGCTCAGCAGTATT
NM_201543.2       GCAGTAAATGGCGTACACAGCCTGGAGTACAAACACAGATTTAAAGAGCTCAGCAGTATT
                  ************************************************************

HsCD00441250      GACACGCTGGAAATTAATGGAGACATCCACTTACTGGAAGTAAGGAGCTGG---
NM_201544.2       GACACGCTGGAAATTAATGGAGACATCCACTTACTGGAAGTAAGGAGCTGGTAG
NM_201543.2       GACACGCTGGAAATTAATGGAGACATCCACTTACTGGAAGTAAGGAGCTGGTAG
                  ***************************************************   

Protein

SMART domain : GLECT : Galectin
SMART domain : Gal-bind_lectin : Galactoside-binding lectin
HPRD : 09356

Clone

Original Clone ID : ccsbBroad304_10205

Organism


TAX_ID : 9606
Species Specific ID: 3964

Genome

Chromosome : 1
Map Location : 1q43
Ensembl : ENSG00000116977

Reagents

Labome : LGALS8-antibody