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FBXO8 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  FBXO8
Gene Name:  F-box protein 8
Sequence             
Original Clone ID: ccsbBroad304_14109
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00441329

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 957nts         Open reading frame : 1 to 957
Reference Sequence Alignment

ATGGGTCAAGGGTTGTGGAGAGTGGTCAGAAACCAGCAGCTGCAACAAGAAGGCTACAGT
GAGCAAGGCTACCTCACCAGAGAGCAGAGCAGGAGAATGGCTGCGAGCAACATTTCTAAC
ACCAATCATCGTAAACAAGTCCAAGGAGGCATTGACATATATCATCTTTTGAAGGCAAGG
AAATCGAAAGAACAGGAAGGATTCATTAATTTGGAAATGTTGCCTCCTGAGCTAAGCTTT
ACCATCTTGTCCTACCTGAATGCAACTGACCTTTGCTTGGCTTCATGTGTTTGGCAGGAC
CTTGCGAATGATGAACTTCTCTGGCAAGGGTTGTGCAAATCCACTTGGGGTCACTGTTCC
ATATACAATAAGAACCCACCTTTAGGATTTTCTTTTAGAAAATTGTATATGCAGCTGGAT
GAAGGCAGCCTCACCTTTAATGCCAACCCAGATGAGGGAGTGAACTACTTTATGTCCAAG
GGTATCCTGGATGATTCGCCAAAGGAAATAGCAAAGTTTATCTTCTGTACAAGAACACTA
AATTGGAAAAAACTGAGAATCTATCTTGATGAAAGGAGAGATGTCTTGGATGACCTTGTA
ACATTGCATAATTTTAGAAATCAGTTCTTGCCAAATGCACTGAGAGAATTTTTTCGTCAT
ATCCATGCCCCTGAAGAGCGTGGAGAGTATCTTGAAACTCTTATAACAAAGTTCTCACAT
AGATTCTGTGCTTGCAACCCTGATTTAATGCGAGAACTTGGCCTTAGTCCTGATGCTGTC
TATGTACTGTGCTACTCTTTGATTCTACTTTCCATTGACCTCACTAGCCCTCATGTGAAG
AATAAAATGTCAAAAAGGGAATTTATTCGAAATACCCGTCGCGCTGCTCAAAATATTAGT
GAAGATTTTGTAGGGCATCTTTATGACAATATCTACCTTATTGGCCATGTGGCGCA


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
3 errors; insertion or deletion in coding sequence
                                

5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : FBXO8
Symbol Nomenclature : FBXO8
SYNONYM : FBS; FBX8
Designation : F-box only protein 8|F-box protein Fbx8|F-box/SEC7 protein FBS
Full Nomenclature : F-box protein 8
GENEID : 26269
Locus Tag : DC10
HGNC : 13587
MIM : 605649
Vega : OTTHUMG00000160746
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00441329      ATGGGTCAAGGGTTGTGGAGAGTGGTCAGAAACCAGCAGCTGCAACAAGAAGGCTACAGT
NM_012180.2       ATGGGTCAAGGGTTGTGGAGAGTGGTCAGAAACCAGCAGCTGCAACAAGAAGGCTACAGT
                  ************************************************************

HsCD00441329      GAGCAAGGCTACCTCACCAGAGAGCAGAGCAGGAGAATGGCTGCGAGCAACATTTCTAAC
NM_012180.2       GAGCAAGGCTACCTCACCAGAGAGCAGAGCAGGAGAATGGCTGCGAGCAACATTTCTAAC
                  ************************************************************

HsCD00441329      ACCAATCATCGTAAACAAGTCCAAGGAGGCATTGACATATATCATCTTTTGAAGGCAAGG
NM_012180.2       ACCAATCATCGTAAACAAGTCCAAGGAGGCATTGACATATATCATCTTTTGAAGGCAAGG
                  ************************************************************

HsCD00441329      AAATCGAAAGAACAGGAAGGATTCATTAATTTGGAAATGTTGCCTCCTGAGCTAAGCTTT
NM_012180.2       AAATCGAAAGAACAGGAAGGATTCATTAATTTGGAAATGTTGCCTCCTGAGCTAAGCTTT
                  ************************************************************

HsCD00441329      ACCATCTTGTCCTACCTGAATGCAACTGACCTTTGCTTGGCTTCATGTGTTTGGCAGGAC
NM_012180.2       ACCATCTTGTCCTACCTGAATGCAACTGACCTTTGCTTGGCTTCATGTGTTTGGCAGGAC
                  ************************************************************

HsCD00441329      CTTGCGAATGATGAACTTCTCTGGCAAGGGTTGTGCAAATCCACTTGGGGTCACTGTTCC
NM_012180.2       CTTGCGAATGATGAACTTCTCTGGCAAGGGTTGTGCAAATCCACTTGGGGTCACTGTTCC
                  ************************************************************

HsCD00441329      ATATACAATAAGAACCCACCTTTAGGATTTTCTTTTAGAAAATTGTATATGCAGCTGGAT
NM_012180.2       ATATACAATAAGAACCCACCTTTAGGATTTTCTTTTAGAAAATTGTATATGCAGCTGGAT
                  ************************************************************

HsCD00441329      GAAGGCAGCCTCACCTTTAATGCCAACCCAGATGAGGGAGTGAACTACTTTATGTCCAAG
NM_012180.2       GAAGGCAGCCTCACCTTTAATGCCAACCCAGATGAGGGAGTGAACTACTTTATGTCCAAG
                  ************************************************************

HsCD00441329      GGTATCCTGGATGATTCGCCAAAGGAAATAGCAAAGTTTATCTTCTGTACAAGAACACTA
NM_012180.2       GGTATCCTGGATGATTCGCCAAAGGAAATAGCAAAGTTTATCTTCTGTACAAGAACACTA
                  ************************************************************

HsCD00441329      AATTGGAAAAAACTGAGAATCTATCTTGATGAAAGGAGAGATGTCTTGGATGACCTTGTA
NM_012180.2       AATTGGAAAAAACTGAGAATCTATCTTGATGAAAGGAGAGATGTCTTGGATGACCTTGTA
                  ************************************************************

HsCD00441329      ACATTGCATAATTTTAGAAATCAGTTCTTGCCAAATGCACTGAGAGAATTTTTTCGTCAT
NM_012180.2       ACATTGCATAATTTTAGAAATCAGTTCTTGCCAAATGCACTGAGAGAATTTTTTCGTCAT
                  ************************************************************

HsCD00441329      ATCCATGCCCCTGAAGAGCGTGGAGAGTATCTTGAAACTCTTATAACAAAGTTCTCACAT
NM_012180.2       ATCCATGCCCCTGAAGAGCGTGGAGAGTATCTTGAAACTCTTATAACAAAGTTCTCACAT
                  ************************************************************

HsCD00441329      AGATTCTGTGCTTGCAACCCTGATTTAATGCGAGAACTTGGCCTTAGTCCTGATGCTGTC
NM_012180.2       AGATTCTGTGCTTGCAACCCTGATTTAATGCGAGAACTTGGCCTTAGTCCTGATGCTGTC
                  ************************************************************

HsCD00441329      TATGTACTGTGCTACTCTTTGATTCTACTTTCCATTGACCTCACTAGCCCTCATGTGAAG
NM_012180.2       TATGTACTGTGCTACTCTTTGATTCTACTTTCCATTGACCTCACTAGCCCTCATGTGAAG
                  ************************************************************

HsCD00441329      AATAAAATGTCAAAAAGGGAATTTATTCGAAATACCCGTCGCGCTGCTCAAAATATTAGT
NM_012180.2       AATAAAATGTCAAAAAGGGAATTTATTCGAAATACCCGTCGCGCTGCTCAAAATATTAGT
                  ************************************************************

HsCD00441329      GAAGATTTTGTAGGGCATCTTTATGACAATATCTACCTTATTGGCCATGTGGCGCA----
NM_012180.2       GAAGATTTTGTAGGGCATCTTTATGACAATATCTACCTTATTGGCCATGTGGCTGCATAA
                  *****************************************************  .    

Pathways

NCI : Arf6 signaling events

Protein

SMART domain : Sec7 : Sec7 domain
HPRD : 07293

Clone

Original Clone ID : ccsbBroad304_14109

Organism


TAX_ID : 9606
Species Specific ID: 26269

Genome

Chromosome : 4
Map Location : 4q34.1
Ensembl : ENSG00000164117

Reagents

Labome : FBXO8-antibody