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ARHGEF25 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ARHGEF25
Gene Name:  Rho guanine nucleotide exchange factor (GEF) 25
Sequence             
Original Clone ID: ccsbBroad304_13049
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00441667

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1423nts         Open reading frame : 1 to 1423
Reference Sequence Alignment

ATGTTGGAGCCAGCTCTAGCCACAGGAGAGGAGCTGCCGGAACTGACCTTGCTGACCACA
CTGTTGGAGGGCCCTGGAGATAAGACGCAGCCACCTGAAGAGGAGACTTTGTCCCAAGCC
CCTGAGAGTGAGGAGGAACAGAAGAAGAAGGCTCTGGAAAGGAGTATGTATGTCCTGAGT
GAACTGGTAGAAACAGAGAAAATGTACGTGGACGACTTGGGGCAGATTGTGGAGGGTTAT
ATGGCCACCATGGCTGCTCAGGGGGTCCCCGAGAGTCTTCGAGGCCGTGACAGGATTGTG
TTTGGGAATATCCAGCAAATCTATGAGTGGCACCGAGACTATTTCTTGCAAGAGCTACAA
CGGTGTCTGAAAGATCCTGATTGGCTGGCTCAGCTATTCATCAAACACGAGCGCCGGCTG
CATATGTATGTGGTGTACTGTCAGAATAAGCCCAAGTCAGAGCATGTGGTGTCAGAGTTT
GGGGACAGCTACTTTGAGGAGCTCCGGCAGCAGCTGGGGCACCGCCTGCAGCTGAACGAC
CTCCTCATCAAACCTGTGCAGCGGATCATGAAATACCAGCTGCTGCTCAAGGATTTTCTC
AAGTATTACAATAGAGCTGGGATGGATACTGCAGACCTAGAGCAAGCTGTGGAGGTCATG
TGCTTTGTGCCCAAGCGCTGCAACGATATGATGACGCTGGGGAGATTGCGGGGATTTGAG
GGCAAACTGACTGCTCAGGGGAAGCTCTTGGGCCAGGACACTTTCTGGGTCACCGAGCCT
GAGGCTGGAGGGCTGCTATCTTCCCGAGGTCGAGAGAGGCGCGTCTTCCTCTTTGAGCAA
ATCATCATCTTCAGTGAAGCCCTGGGAGGAGGAGTGAGAGGTGGAACACAGCCTGGATAT
GTATACAAGAACAGCATTAAGGTGAGCTGCCTGGGACTGGAGGGGAACCTCCAAGGTGAC
CCTTGCCGCTTTGCACTGACCTCCAGAGGGCCAGAGGGTGGGATCCAGCGCTATGTCCTG
CAGGCTGCAGACCCTGCTATCAGTCAGGCCTGGATCAAGCATGTGGCTCAGATCTTGGAG
AGCCAACGGGACTTCCTCAACGCATTGCAGTCACCCATTGAGTACCAGAGACGGGAGAGC
CAGACCAACAGCCTGGGGCGGCCAAGAGGGCCTGGAGTGGGGAGCCCTGGAAGAATTCGG
CTTGGAGATCAGGCCCAGGGCAGCACACACACACCCATCAATGGCTCTCTCCCCTCTCTG
CTGCTGTCACCCAAAGGGGAGGTGGCCAGAGCCCTCTTGCCACTGGATAAACAGGCCCTT
GGTGACATCCCCCAGGCTCCCCATGACTCTCCTCCAGTCTCTCCAACTCCAAAAACCCCT
CCCTGCCAAGCCAGACTTGCCAAGCTGGATGAAGATGAGCTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ARHGEF25
Symbol Nomenclature : ARHGEF25
SYNONYM : GEFT; p63RhoGEF
Designation : RAC/CDC42 exchange factor|RhoA/RAC/CDC42 exchange factor|guanine nucleotide exchange factor GEFT|rac/Cdc42/Rho exchange factor GEFT|rho guanine nucleotide exchange factor 25|rhoA/Rac/Cdc42 guanine nucleotide exchange factor GEFT
Full Nomenclature : Rho guanine nucleotide exchange factor (GEF) 25
GENEID : 115557
HGNC : 30275
MIM : 610215
Vega : OTTHUMG00000152516
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00441667        ------------------------------------------------------------
NM_182947.3         ------------------------------------------------------------
NM_001111270.2      ATGAAGCCCCCGGACCGCCCCGCCCCTGGCCGCACTGACCGGATACTGGGGGTCATGGGG
                                                                                

HsCD00441667        ------------------------------------------------------------
NM_182947.3         ---------------------------------------------------------ATG
NM_001111270.2      GGCATGCTGCGCGCATGCGCCCTCCCTGGGCAGGAGGGGCCCCCAAGGAGAAGCCCTCTA
                                                                                

HsCD00441667        ------------------------------------------------------------
NM_182947.3         CGGGGGGGGCACAAAGGGGGTCGCTGTGCCTGTCCCCGTGTGATCCGAAAAGTGCTGGCA
NM_001111270.2      GGGTTGGTGGGTACCGAGCCAGAGTCTGAACGTACGGAGGGAGATCACAGAAGGGATCGC
                                                                                

HsCD00441667        ------------------------------------------------------------
NM_182947.3         AAATGCGGCTGCTGCTTCGCCCGGGGGGGACGTGAATCCTATTCCATTGCGGGCAGTGAG
NM_001111270.2      GAACATGAGGTCCTCGCCGGGGCTCTGCAGCCCGAATCCTATTCCATTGCGGGCAGTGAG
                                                                                

HsCD00441667        ------------------------------------------------------------
NM_182947.3         GGGAGTATATCGGCTTCTGCTGCCTCCGGTCTGGCTGCCCCCTCTGGCCCCAGCTCTGGC
NM_001111270.2      GGGAGTATATCGGCTTCTGCTGCCTCCGGTCTGGCTGCCCCCTCTGGCCCCAGCTCTGGC
                                                                                

HsCD00441667        ------------------------------------------------------------
NM_182947.3         CTCAGCTCTGGCCCCTGTTCCCCAGGCCCCCCAGGGCCCGTCAGTGGCCTGAGGAGATGG
NM_001111270.2      CTCAGCTCTGGCCCCTGTTCCCCAGGCCCCCCAGGGCCCGTCAGTGGCCTGAGGAGATGG
                                                                                

HsCD00441667        ------------------------------------------------------------
NM_182947.3         TTGGATCATTCCAAACATTGTCTCAGTGTGGAAACTGAGGCAGACAGTGGTCAGGCAGGA
NM_001111270.2      TTGGATCATTCCAAACATTGTCTCAGTGTGGAAACTGAGGCAGACAGTGGTCAGGCAGGA
                                                                                

HsCD00441667        ---------------ATGTTGGAGCCAGCTCTAGCCACAGGAGAGGAGCTGCCGGAACTG
NM_182947.3         CCATATGAGAACTGGATGTTGGAGCCAGCTCTAGCCACAGGAGAGGAGCTGCCGGAACTG
NM_001111270.2      CCATATGAGAACTGGATGTTGGAGCCAGCTCTAGCCACAGGAGAGGAGCTGCCGGAACTG
                                   *********************************************

HsCD00441667        ACCTTGCTGACCACACTGTTGGAGGGCCCTGGAGATAAGACGCAGCCACCTGAAGAGGAG
NM_182947.3         ACCTTGCTGACCACACTGTTGGAGGGCCCTGGAGATAAGACGCAGCCACCTGAAGAGGAG
NM_001111270.2      ACCTTGCTGACCACACTGTTGGAGGGCCCTGGAGATAAGACGCAGCCACCTGAAGAGGAG
                    ************************************************************

HsCD00441667        ACTTTGTCCCAAGCCCCTGAGAGTGAGGAGGAACAGAAGAAGAAGGCTCTGGAAAGGAGT
NM_182947.3         ACTTTGTCCCAAGCCCCTGAGAGTGAGGAGGAACAGAAGAAGAAGGCTCTGGAAAGGAGT
NM_001111270.2      ACTTTGTCCCAAGCCCCTGAGAGTGAGGAGGAACAGAAGAAGAAGGCTCTGGAAAGGAGT
                    ************************************************************

HsCD00441667        ATGTATGTCCTGAGTGAACTGGTAGAAACAGAGAAAATGTACGTGGACGACTTGGGGCAG
NM_182947.3         ATGTATGTCCTGAGTGAACTGGTAGAAACAGAGAAAATGTACGTGGACGACTTGGGGCAG
NM_001111270.2      ATGTATGTCCTGAGTGAACTGGTAGAAACAGAGAAAATGTACGTGGACGACTTGGGGCAG
                    ************************************************************

HsCD00441667        ATTGTGGAGGGTTATATGGCCACCATGGCTGCTCAGGGGGTCCCCGAGAGTCTTCGAGGC
NM_182947.3         ATTGTGGAGGGTTATATGGCCACCATGGCTGCTCAGGGGGTCCCCGAGAGTCTTCGAGGC
NM_001111270.2      ATTGTGGAGGGTTATATGGCCACCATGGCTGCTCAGGGGGTCCCCGAGAGTCTTCGAGGC
                    ************************************************************

HsCD00441667        CGTGACAGGATTGTGTTTGGGAATATCCAGCAAATCTATGAGTGGCACCGAGACTATTTC
NM_182947.3         CGTGACAGGATTGTGTTTGGGAATATCCAGCAAATCTATGAGTGGCACCGAGACTATTTC
NM_001111270.2      CGTGACAGGATTGTGTTTGGGAATATCCAGCAAATCTATGAGTGGCACCGAGACTATTTC
                    ************************************************************

HsCD00441667        TTGCAAGAGCTACAACGGTGTCTGAAAGATCCTGATTGGCTGGCTCAGCTATTCATCAAA
NM_182947.3         TTGCAAGAGCTACAACGGTGTCTGAAAGATCCTGATTGGCTGGCTCAGCTATTCATCAAA
NM_001111270.2      TTGCAAGAGCTACAACGGTGTCTGAAAGATCCTGATTGGCTGGCTCAGCTATTCATCAAA
                    ************************************************************

HsCD00441667        CACGAGCGCCGGCTGCATATGTATGTGGTGTACTGTCAGAATAAGCCCAAGTCAGAGCAT
NM_182947.3         CACGAGCGCCGGCTGCATATGTATGTGGTGTACTGTCAGAATAAGCCCAAGTCAGAGCAT
NM_001111270.2      CACGAGCGCCGGCTGCATATGTATGTGGTGTACTGTCAGAATAAGCCCAAGTCAGAGCAT
                    ************************************************************

HsCD00441667        GTGGTGTCAGAGTTTGGGGACAGCTACTTTGAGGAGCTCCGGCAGCAGCTGGGGCACCGC
NM_182947.3         GTGGTGTCAGAGTTTGGGGACAGCTACTTTGAGGAGCTCCGGCAGCAGCTGGGGCACCGC
NM_001111270.2      GTGGTGTCAGAGTTTGGGGACAGCTACTTTGAGGAGCTCCGGCAGCAGCTGGGGCACCGC
                    ************************************************************

HsCD00441667        CTGCAGCTGAACGACCTCCTCATCAAACCTGTGCAGCGGATCATGAAATACCAGCTGCTG
NM_182947.3         CTGCAGCTGAACGACCTCCTCATCAAACCTGTGCAGCGGATCATGAAATACCAGCTGCTG
NM_001111270.2      CTGCAGCTGAACGACCTCCTCATCAAACCTGTGCAGCGGATCATGAAATACCAGCTGCTG
                    ************************************************************

HsCD00441667        CTCAAGGATTTTCTCAAGTATTACAATAGAGCTGGGATGGATACTGCAGACCTAGAGCAA
NM_182947.3         CTCAAGGATTTTCTCAAGTATTACAATAGAGCTGGGATGGATACTGCAGACCTAGAGCAA
NM_001111270.2      CTCAAGGATTTTCTCAAGTATTACAATAGAGCTGGGATGGATACTGCAGACCTAGAGCAA
                    ************************************************************

HsCD00441667        GCTGTGGAGGTCATGTGCTTTGTGCCCAAGCGCTGCAACGATATGATGACGCTGGGGAGA
NM_182947.3         GCTGTGGAGGTCATGTGCTTTGTGCCCAAGCGCTGCAACGATATGATGACGCTGGGGAGA
NM_001111270.2      GCTGTGGAGGTCATGTGCTTTGTGCCCAAGCGCTGCAACGATATGATGACGCTGGGGAGA
                    ************************************************************

HsCD00441667        TTGCGGGGATTTGAGGGCAAACTGACTGCTCAGGGGAAGCTCTTGGGCCAGGACACTTTC
NM_182947.3         TTGCGGGGATTTGAGGGCAAACTGACTGCTCAGGGGAAGCTCTTGGGCCAGGACACTTTC
NM_001111270.2      TTGCGGGGATTTGAGGGCAAACTGACTGCTCAGGGGAAGCTCTTGGGCCAGGACACTTTC
                    ************************************************************

HsCD00441667        TGGGTCACCGAGCCTGAGGCTGGAGGGCTGCTATCTTCCCGAGGTCGAGAGAGGCGCGTC
NM_182947.3         TGGGTCACCGAGCCTGAGGCTGGAGGGCTGCTGTCTTCCCGAGGTCGAGAGAGGCGCGTC
NM_001111270.2      TGGGTCACCGAGCCTGAGGCTGGAGGGCTGCTGTCTTCCCGAGGTCGAGAGAGGCGCGTC
                    ********************************.***************************

HsCD00441667        TTCCTCTTTGAGCAAATCATCATCTTCAGTGAAGCCCTGGGAGGAGGAGTGAGAGGTGGA
NM_182947.3         TTCCTCTTTGAGCAAATCATCATCTTCAGTGAAGCCCTGGGAGGAGGAGTGAGAGGTGGA
NM_001111270.2      TTCCTCTTTGAGCAAATCATCATCTTCAGTGAAGCCCTGGGAGGAGGAGTGAGAGGTGGA
                    ************************************************************

HsCD00441667        ACACAGCCTGGATATGTATACAAGAACAGCATTAAGGTGAGCTGCCTGGGACTGGAGGGG
NM_182947.3         ACACAGCCTGGATATGTATACAAGAACAGCATTAAGGTGAGCTGCCTGGGACTGGAGGGG
NM_001111270.2      ACACAGCCTGGATATGTATACAAGAACAGCATTAAGGTGAGCTGCCTGGGACTGGAGGGG
                    ************************************************************

HsCD00441667        AACCTCCAAGGTGACCCTTGCCGCTTTGCACTGACCTCCAGAGGGCCAGAGGGTGGGATC
NM_182947.3         AACCTCCAAGGTGACCCTTGCCGCTTTGCACTGACCTCCAGAGGGCCAGAGGGTGGGATC
NM_001111270.2      AACCTCCAAGGTGACCCTTGCCGCTTTGCACTGACCTCCAGAGGGCCAGAGGGTGGGATC
                    ************************************************************

HsCD00441667        CAGCGCTATGTCCTGCAGGCTGCAGACCCTGCTATCAGTCAGGCCTGGATCAAGCATGTG
NM_182947.3         CAGCGCTATGTCCTGCAGGCTGCAGACCCTGCTATCAGTCAGGCCTGGATCAAGCATGTG
NM_001111270.2      CAGCGCTATGTCCTGCAGGCTGCAGACCCTGCTATCAGTCAGGCCTGGATCAAGCATGTG
                    ************************************************************

HsCD00441667        GCTCAGATCTTGGAGAGCCAACGGGACTTCCTCAACGCATTGCAGTCACCCATTGAGTAC
NM_182947.3         GCTCAGATCTTGGAGAGCCAACGGGACTTCCTCAACGCATTGCAGTCACCCATTGAGTAC
NM_001111270.2      GCTCAGATCTTGGAGAGCCAACGGGACTTCCTCAACGCATTGCAGTCACCCATTGAGTAC
                    ************************************************************

HsCD00441667        CAGAGACGGGAGAGCCAGACCAACAGCCTGGGGCGGCCAAGAGGGCCTGGAGTGGGGAGC
NM_182947.3         CAGAGACGGGAGAGCCAGACCAACAGCCTGGGGCGGCCAAGAGGGCCTGGAGTGGGGAGC
NM_001111270.2      CAGAGACGGGAGAGCCAGACCAACAGCCTGGGGCGGCCAAGAGGGCCTGGAGTGGGGAGC
                    ************************************************************

HsCD00441667        CCTGGAAGAATTCGGCTTGGAGATCAGGCCCAGGGCAGCACACACACACCCATCAATGGC
NM_182947.3         CCTGGAAGAATTCGGCTTGGAGATCAGGCCCAGGGCAGCACACACACACCCATCAATGGC
NM_001111270.2      CCTGGAAGAATTCGGCTTGGAGATCAGGCCCAGGGCAGCACACACACACCCATCAATGGC
                    ************************************************************

HsCD00441667        TCTCTCCCCTCTCTGCTGCTGTCACCCAAAGGGGAGGTGGCCAGAGCCCTCTTGCCACTG
NM_182947.3         TCTCTCCCCTCTCTGCTGCTGTCACCCAAAGGGGAGGTGGCCAGAGCCCTCTTGCCACTG
NM_001111270.2      TCTCTCCCCTCTCTGCTGCTGTCACCCAAAGGGGAGGTGGCCAGAGCCCTCTTGCCACTG
                    ************************************************************

HsCD00441667        GATAAACAGGCCCTTGGTGACATCCCCCAGGCTCCCCATGACTCTCCTCCAGTCTCTCCA
NM_182947.3         GATAAACAGGCCCTTGGTGACATCCCCCAGGCTCCCCATGACTCTCCTCCAGTCTCTCCA
NM_001111270.2      GATAAACAGGCCCTTGGTGACATCCCCCAGGCTCCCCATGACTCTCCTCCAGTCTCTCCA
                    ************************************************************

HsCD00441667        ACTCCAAAAACCCCTCCCTGCCAAGCCAGACTTGCCAAGCTGGATGAAGATGAGCTG---
NM_182947.3         ACTCCAAAAACCCCTCCCTGCCAAGCCAGACTTGCCAAGCTGGATGAAGATGAGCTGTAA
NM_001111270.2      ACTCCAAAAACCCCTCCCTGCCAAGCCAGACTTGCCAAGCTGGATGAAGATGAGCTGTAA
                    *********************************************************   

Pathways

NCI : Regulation of CDC42 activity
NCI : Regulation of RAC1 activity
NCI : Regulation of RhoA activity
Reactome : G alpha (q) signalling events
Reactome : GPCR downstream signaling
Reactome : Gastrin-CREB signalling pathway via PKC and MAPK
Reactome : Signal Transduction
Reactome : Signaling by GPCR

Clone

Original Clone ID : ccsbBroad304_13049

Organism


TAX_ID : 9606
Species Specific ID: 115557

Genome

Chromosome : 12
Map Location : 12q13.3
Ensembl : ENSG00000240771

Reagents

Labome : ARHGEF25-antibody