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KBTBD7 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  KBTBD7
Gene Name:  kelch repeat and BTB (POZ) domain containing 7
Sequence             
Original Clone ID: ccsbBroad304_04326
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00441815

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 2053nts         Open reading frame : 1 to 2053
Reference Sequence Alignment

ATGCAGTCCCGGGAAGACGTCCCGCGCTCTCGCCGCCTCGCCAGTCCCCGTGGTGGGAGG
CGGCCCAAGAGGATTTCCAAGCCCTCGGTTTCGGCCTTTTTCACGGGTCCAGAGGAGTTA
AAGGACACGGCCCATTCTGCAGCCCTGCTGGCACAGCTCAAGTCCTTCTACGACGCGCGG
CTGCTGTGTGATGTGACCATCGAGGTGGTGACGCCTGGCAGCGGGCCTGGCACGGGTCGC
CTCTTTTCCTGCAATCGCAACGTGCTAGCAGCTGCGTGTCCCTACTTCAAGAGCATGTTC
ACAGGTGGCATGTACGAGAGCCAGCAGGCCAGCGTGACCATGCACGATGTGGACGCCGAG
TCCTTCGAGGTGTTGGTCGACTACTGCTACACGGGTCGTGTGTCTCTCAGTGAGGCCAAT
GTGCAGCGCCTGTACGCGGCCTCCGACATGCTACAGCTGGAATATGTGCGGGAAGCCTGT
GCCTCCTTCTTAGCCCGACGTCTTGACCTGACCAACTGCACCGCCATCCTCAAGTTTGCA
GACGCCTTCGACCATCACAAGCTTCGATCTCAGGCCCAGTCCTACATAGCTCACAACTTC
AAGCAGCTCAGCCGAATGGGTTCAATTCGGGAGGAGACTCTAGCAGATCTAACCCTGGCC
CAGCTGCTGGCTGTCCTACGCCTGGATAGTCTGGACATAGAGAGTGAGCGGACTGTATGC
CATGTAGCTGTGCAGTGGCTGGAGGCTGCTGCCAAAGAGCGGGGTCCCAGTGCTGCAGAA
GTCTTCAAGTGCGTGCGCTGGATGCACTTCACTGAAGAAGATCAGGACTACTTAGAAGGG
CTGCTGACCAAGCCCATCGTGAAGAAGTACTGCCTGGACGTTATTGAAGGGGCCCTGCAG
ATGCGCTATGGTGACCTGTTGTACAAGTCTCTGGTGCCAGTGCCAAACAGCAGCAGCAGC
AGTAGCAGCAGCAACTCTCTTGTATCTGCAGCAGAAAATCCACCCCAGAGACTGGGTATG
TGTGCCAAGGAGATGGTGATCTTCTTTGGACATCCTAGAGATCCCTTTCTCTGCTATGAC
CCTTACTCGGGGGACATTTACACAATGCCATCCCCTTTGACCAGCTTTGCTCACACTAAG
ACTGTCACCTCCTCAGCTGTCTGTGTGTCCCCAGACCATGACATCTATCTAGCTGCTCAG
CCCAGGAAAGACCTCTGGGTGTATAAACCAGCTCAGAATAGTTGGCAGCAACTTGCAGAT
CGCTTGCTGTGTCGTGAGGGCATGGATGTGGCATATCTCAATGGCTACATCTACATTTTG
GGGGGACGAGACCCTATTACTGGAGTTAAGTTGAAGGAAGTGGAATGCTACAGTGTTCAG
AGAAACCAGTGGGCATTGGTGGCTCCTGTCCCTCATTCCTTCTATTCCTTTGAACTCATA
GTGGTTCAGAACTATCTTTATGCTGTCAACAGTAAGCGCATGCTTTGCTATGATCCTAGC
CACAATATGTGGCTGAACTGTGCTTCTCTTAAACGTAGTGACTTTCAGGAAGCATGTGTC
TTCAATGATGAAATCTATTGTATCTGTGACATCCCAGTCATGAAGGTCTACAACCCAGCT
AGGGGAGAATGGAGGCGGATTAGTAATATTCCTTTGGATTCAGAGACCCACAACTACCAG
ATTGTCAATCATGACCAAAAGTTGCTTCTCATCACTTCTACAACCCCACAATGGAAAAAG
AACCGAGTGACAGTGTATGAGTATGATACTAGGGAAGATCAGTGGATTAATATAGGTACC
ATGTTAGGCCTTTTGCAGTTTGACTCTGGCTTTATTTGCCTTTGTGCTCGTGTTTATCCT
TCCTGCCTTGAACCTGGTCAGAGTTTTATTACTGAGGAAGATGATGCACGGAGTGAGTCT
AGTACTGAATGGGACTTAGATGGATTCAGTGAGCTGGACTCTGAGTCAGGAAGTTCAAGT
TCTTTTTCAGATGATGAAGTCTGGGTGCAAGTAGCACCTCAGCGAAATGCACAGGATCAG
CAGGGTTCTTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : KBTBD7
Symbol Nomenclature : KBTBD7
Designation : kelch repeat and BTB domain-containing protein 7
Full Nomenclature : kelch repeat and BTB (POZ) domain containing 7
GENEID : 84078
GenBank Accession : DQ893994
HGNC : 25266
Target GenBank: DQ893994

 

Reference Sequence Alignment

  

HsCD00441815      ATGCAGTCCCGGGAAGACGTCCCGCGCTCTCGCCGCCTCGCCAGTCCCCGTGGTGGGAGG
NM_032138.4       ATGCAGTCCCGGGAAGACGTCCCGCGCTCTCGCCGCCTCGCCAGTCCCCGTGGTGGGAGG
                  ************************************************************

HsCD00441815      CGGCCCAAGAGGATTTCCAAGCCCTCGGTTTCGGCCTTTTTCACGGGTCCAGAGGAGTTA
NM_032138.4       CGGCCCAAGAGGATTTCCAAGCCCTCGGTTTCGGCCTTTTTCACGGGTCCAGAGGAGTTA
                  ************************************************************

HsCD00441815      AAGGACACGGCCCATTCTGCAGCCCTGCTGGCACAGCTCAAGTCCTTCTACGACGCGCGG
NM_032138.4       AAGGACACGGCCCATTCTGCAGCCCTGCTGGCACAGCTCAAGTCCTTCTACGACGCGCGG
                  ************************************************************

HsCD00441815      CTGCTGTGTGATGTGACCATCGAGGTGGTGACGCCTGGCAGCGGGCCTGGCACGGGTCGC
NM_032138.4       CTGCTGTGTGATGTGACCATCGAGGTGGTGACGCCTGGCAGCGGGCCTGGCACGGGTCGC
                  ************************************************************

HsCD00441815      CTCTTTTCCTGCAATCGCAACGTGCTAGCAGCTGCGTGTCCCTACTTCAAGAGCATGTTC
NM_032138.4       CTCTTTTCCTGCAATCGCAACGTGCTAGCAGCTGCGTGTCCCTACTTCAAGAGCATGTTC
                  ************************************************************

HsCD00441815      ACAGGTGGCATGTACGAGAGCCAGCAGGCCAGCGTGACCATGCACGATGTGGACGCCGAG
NM_032138.4       ACAGGTGGCATGTACGAGAGCCAGCAGGCCAGCGTGACCATGCACGATGTGGACGCCGAG
                  ************************************************************

HsCD00441815      TCCTTCGAGGTGTTGGTCGACTACTGCTACACGGGTCGTGTGTCTCTCAGTGAGGCCAAT
NM_032138.4       TCCTTCGAGGTGTTGGTCGACTACTGCTACACGGGTCGTGTGTCTCTCAGTGAGGCCAAT
                  ************************************************************

HsCD00441815      GTGCAGCGCCTGTACGCGGCCTCCGACATGCTACAGCTGGAATATGTGCGGGAAGCCTGT
NM_032138.4       GTGCAGCGCCTGTACGCGGCCTCCGACATGCTACAGCTGGAATATGTGCGGGAAGCCTGT
                  ************************************************************

HsCD00441815      GCCTCCTTCTTAGCCCGACGTCTTGACCTGACCAACTGCACCGCCATCCTCAAGTTTGCA
NM_032138.4       GCCTCCTTCTTAGCCCGACGTCTTGACCTGACCAACTGCACCGCCATCCTCAAGTTTGCA
                  ************************************************************

HsCD00441815      GACGCCTTCGACCATCACAAGCTTCGATCTCAGGCCCAGTCCTACATAGCTCACAACTTC
NM_032138.4       GACGCCTTCGACCATCACAAGCTTCGATCTCAGGCCCAGTCCTACATAGCTCACAACTTC
                  ************************************************************

HsCD00441815      AAGCAGCTCAGCCGAATGGGTTCAATTCGGGAGGAGACTCTAGCAGATCTAACCCTGGCC
NM_032138.4       AAGCAGCTCAGCCGAATGGGTTCAATTCGGGAGGAGACTCTAGCAGATCTAACCCTGGCC
                  ************************************************************

HsCD00441815      CAGCTGCTGGCTGTCCTACGCCTGGATAGTCTGGACATAGAGAGTGAGCGGACTGTATGC
NM_032138.4       CAGCTGCTGGCTGTCCTACGCCTGGATAGTCTGGACATAGAGAGTGAGCGGACTGTATGC
                  ************************************************************

HsCD00441815      CATGTAGCTGTGCAGTGGCTGGAGGCTGCTGCCAAAGAGCGGGGTCCCAGTGCTGCAGAA
NM_032138.4       CATGTAGCTGTGCAGTGGCTGGAGGCTGCTGCCAAAGAGCGGGGTCCCAGTGCTGCAGAA
                  ************************************************************

HsCD00441815      GTCTTCAAGTGCGTGCGCTGGATGCACTTCACTGAAGAAGATCAGGACTACTTAGAAGGG
NM_032138.4       GTCTTCAAGTGCGTGCGCTGGATGCACTTCACTGAAGAAGATCAGGACTACTTAGAAGGG
                  ************************************************************

HsCD00441815      CTGCTGACCAAGCCCATCGTGAAGAAGTACTGCCTGGACGTTATTGAAGGGGCCCTGCAG
NM_032138.4       CTGCTGACCAAGCCCATCGTGAAGAAGTACTGCCTGGACGTTATTGAAGGGGCCCTGCAG
                  ************************************************************

HsCD00441815      ATGCGCTATGGTGACCTGTTGTACAAGTCTCTGGTGCCAGTGCCAAACAGCAGCAGCAGC
NM_032138.4       ATGCGCTATGGTGACCTGTTGTACAAGTCTCTGGTGCCAGTGCCAAACAGCAGCAGCAGC
                  ************************************************************

HsCD00441815      AGTAGCAGCAGCAACTCTCTTGTATCTGCAGCAGAAAATCCACCCCAGAGACTGGGTATG
NM_032138.4       AGTAGCAGCAGCAACTCTCTTGTATCTGCAGCAGAAAATCCACCCCAGAGACTGGGTATG
                  ************************************************************

HsCD00441815      TGTGCCAAGGAGATGGTGATCTTCTTTGGACATCCTAGAGATCCCTTTCTCTGCTATGAC
NM_032138.4       TGTGCCAAGGAGATGGTGATCTTCTTTGGACATCCTAGAGATCCCTTTCTCTGCTATGAC
                  ************************************************************

HsCD00441815      CCTTACTCGGGGGACATTTACACAATGCCATCCCCTTTGACCAGCTTTGCTCACACTAAG
NM_032138.4       CCTTACTCGGGGGACATTTACACAATGCCATCCCCTTTGACCAGCTTTGCTCACACTAAG
                  ************************************************************

HsCD00441815      ACTGTCACCTCCTCAGCTGTCTGTGTGTCCCCAGACCATGACATCTATCTAGCTGCTCAG
NM_032138.4       ACTGTCACCTCCTCAGCTGTCTGTGTGTCCCCAGACCATGACATCTATCTAGCTGCTCAG
                  ************************************************************

HsCD00441815      CCCAGGAAAGACCTCTGGGTGTATAAACCAGCTCAGAATAGTTGGCAGCAACTTGCAGAT
NM_032138.4       CCCAGGAAAGACCTCTGGGTGTATAAACCAGCTCAGAATAGTTGGCAGCAACTTGCAGAT
                  ************************************************************

HsCD00441815      CGCTTGCTGTGTCGTGAGGGCATGGATGTGGCATATCTCAATGGCTACATCTACATTTTG
NM_032138.4       CGCTTGCTGTGTCGTGAGGGCATGGATGTGGCATATCTCAATGGCTACATCTACATTTTG
                  ************************************************************

HsCD00441815      GGGGGACGAGACCCTATTACTGGAGTTAAGTTGAAGGAAGTGGAATGCTACAGTGTTCAG
NM_032138.4       GGGGGACGAGACCCTATTACTGGAGTTAAGTTGAAGGAAGTGGAATGCTACAGTGTTCAG
                  ************************************************************

HsCD00441815      AGAAACCAGTGGGCATTGGTGGCTCCTGTCCCTCATTCCTTCTATTCCTTTGAACTCATA
NM_032138.4       AGAAACCAGTGGGCATTGGTGGCTCCTGTCCCTCATTCCTTCTATTCCTTTGAACTCATA
                  ************************************************************

HsCD00441815      GTGGTTCAGAACTATCTTTATGCTGTCAACAGTAAGCGCATGCTTTGCTATGATCCTAGC
NM_032138.4       GTGGTTCAGAACTATCTTTATGCTGTCAACAGTAAGCGCATGCTTTGCTATGATCCTAGC
                  ************************************************************

HsCD00441815      CACAATATGTGGCTGAACTGTGCTTCTCTTAAACGTAGTGACTTTCAGGAAGCATGTGTC
NM_032138.4       CACAATATGTGGCTGAACTGTGCTTCTCTTAAACGTAGTGACTTTCAGGAAGCATGTGTC
                  ************************************************************

HsCD00441815      TTCAATGATGAAATCTATTGTATCTGTGACATCCCAGTCATGAAGGTCTACAACCCAGCT
NM_032138.4       TTCAATGATGAAATCTATTGTATCTGTGACATCCCAGTCATGAAGGTCTACAACCCAGCT
                  ************************************************************

HsCD00441815      AGGGGAGAATGGAGGCGGATTAGTAATATTCCTTTGGATTCAGAGACCCACAACTACCAG
NM_032138.4       AGGGGAGAATGGAGGCGGATTAGTAATATTCCTTTGGATTCAGAGACCCACAACTACCAG
                  ************************************************************

HsCD00441815      ATTGTCAATCATGACCAAAAGTTGCTTCTCATCACTTCTACAACCCCACAATGGAAAAAG
NM_032138.4       ATTGTCAATCATGACCAAAAGTTGCTTCTCATCACTTCTACAACCCCACAATGGAAAAAG
                  ************************************************************

HsCD00441815      AACCGAGTGACAGTGTATGAGTATGATACTAGGGAAGATCAGTGGATTAATATAGGTACC
NM_032138.4       AACCGAGTGACAGTGTATGAGTATGATACTAGGGAAGATCAGTGGATTAATATAGGTACC
                  ************************************************************

HsCD00441815      ATGTTAGGCCTTTTGCAGTTTGACTCTGGCTTTATTTGCCTTTGTGCTCGTGTTTATCCT
NM_032138.4       ATGTTAGGCCTTTTGCAGTTTGACTCTGGCTTTATTTGCCTTTGTGCTCGTGTTTATCCT
                  ************************************************************

HsCD00441815      TCCTGCCTTGAACCTGGTCAGAGTTTTATTACTGAGGAAGATGATGCACGGAGTGAGTCT
NM_032138.4       TCCTGCCTTGAACCTGGTCAGAGTTTTATTACTGAGGAAGATGATGCACGGAGTGAGTCT
                  ************************************************************

HsCD00441815      AGTACTGAATGGGACTTAGATGGATTCAGTGAGCTGGACTCTGAGTCAGGAAGTTCAAGT
NM_032138.4       AGTACTGAATGGGACTTAGATGGATTCAGTGAGCTGGACTCTGAGTCAGGAAGTTCAAGT
                  ************************************************************

HsCD00441815      TCTTTTTCAGATGATGAAGTCTGGGTGCAAGTAGCACCTCAGCGAAATGCACAGGATCAG
NM_032138.4       TCTTTTTCAGATGATGAAGTCTGGGTGCAAGTAGCACCTCAGCGAAATGCACAGGATCAG
                  ************************************************************

HsCD00441815      CAGGGTTCTTTG---
NM_032138.4       CAGGGTTCTTTGTAA
                  ************   

Protein

SMART domain : BTB : Broad-Complex, Tramtrack and Bric a brac
SMART domain : Kelch :
SMART domain : BACK : BTB And C-terminal Kelch
HPRD : 13759

Clone

Original Clone ID : ccsbBroad304_04326

Organism


TAX_ID : 9606
Species Specific ID: 84078

Genome

Chromosome : 13
Map Location : 13q14.11

Reagents

Labome : KBTBD7-antibody