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FGF12 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  FGF12
Gene Name:  fibroblast growth factor 12
Sequence             
Original Clone ID: ccsbBroad304_06210
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00441911

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 544nts         Open reading frame : 1 to 544
Reference Sequence Alignment

ATGGAGAGCAAAGAACCCCAGCTAAAAGGGATTGTGACAAGGTTATTCAGCCAGCAGGGA
TACTTCCTGCAGATGCACCCAGATGGTACCATTGATGGGACCAAGGACGAAAACAGCGAC
TACACTCTCTTCAATCTAATTCCCGTGGGCCTGCGTGTAGTGGCCATCCAAGGAGTGAAG
GCTAGCCTCTATGTGGCCATGAATGGTGAAGGCTATCTCTACAGTTCAGATGTTTTCACT
CCAGAATGCAAATTCAAGGAATCTGTGTTTGAAAACTACTATGTGATCTATTCTTCCACA
CTGTACCGCCAGCAAGAATCAGGCCGAGCTTGGTTTCTGGGACTCAATAAAGAAGGTCAA
ATTATGAAGGGGAACAGAGTGAAGAAAACCAAGCCCTCATCACATTTTGTACCGAAACCT
ATTGAAGTGTGTATGTACAGAGAACAATCGCTACATGAAATTGGAGAAAAACAAGGGCGT
TCAAGGAAAAGTTCTGGAACACCAACCATGAATGGAGGCAAAGTTGTGAATCAAGATTCA
ACA


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : FGF12
Symbol Nomenclature : FGF12
SYNONYM : FGF12B; FHF1
Designation : FGF-12|FHF-1|fibroblast growth factor 12B|fibroblast growth factor FGF-12b|fibroblast growth factor homologous factor 1|myocyte-activating factor
Full Nomenclature : fibroblast growth factor 12
GENEID : 2257
GenBank Accession : DQ893775
HGNC : 3668
MIM : 601513
Vega : OTTHUMG00000156132
Target GenBank: DQ893775

 

Reference Sequence Alignment

  

HsCD00441911        ------------------------------------------------------------
NM_004113.5         ------------------------------------------------------------
XM_005247227.1      --------------ATGAAGATTCTTGAGCTTTCTCTGAAGAAAGGAGTGCATATGCTGC
NM_021032.4         ATGGCTGCGGCGATAGCCAGCTCCTTGATCCGGCAGAAGCGGCAGGCGAGGGAGTCCAAC
                                                                                

HsCD00441911        ------------------------------------------------------------
NM_004113.5         ------------------------------------------------------------
XM_005247227.1      AGTGTCTCTGTGGAAGGAGCCTG-------------------------------------
NM_021032.4         AGCGACCGAGTGTCGGCCTCCAAGCGCCGCTCCAGCCCCAGCAAAGACGGGCGCTCCCTG
                                                                                

HsCD00441911        ------------------------------------------------------------
NM_004113.5         ------------------------------------------------------------
XM_005247227.1      ---------------------------------------------------------AGG
NM_021032.4         TGCGAGAGGCACGTCCTCGGGGTGTTCAGCAAAGTGCGCTTCTGCAGCGGCCGCAAGAGG
                                                                                

HsCD00441911        ------ATGGAGAGCAAAGAACCCCAGCTAAAAGGGATTGTGACAAGGTTATTCAGCCAG
NM_004113.5         ------ATGGAGAGCAAAGAACCCCAGCTCAAAGGGATTGTGACAAGGTTATTCAGCCAG
XM_005247227.1      TCCAGCGACAGCCCAAGTGAACCCCAGCTCAAAGGGATTGTGACAAGGTTATTCAGCCAG
NM_021032.4         CCGGTGAGGCGGAGACCAGAACCCCAGCTCAAAGGGATTGTGACAAGGTTATTCAGCCAG
                          .   . . .. :***********.******************************

HsCD00441911        CAGGGATACTTCCTGCAGATGCACCCAGATGGTACCATTGATGGGACCAAGGACGAAAAC
NM_004113.5         CAGGGATACTTCCTGCAGATGCACCCAGATGGTACCATTGATGGGACCAAGGACGAAAAC
XM_005247227.1      CAGGGATACTTCCTGCAGATGCACCCAGATGGTACCATTGATGGGACCAAGGACGAAAAC
NM_021032.4         CAGGGATACTTCCTGCAGATGCACCCAGATGGTACCATTGATGGGACCAAGGACGAAAAC
                    ************************************************************

HsCD00441911        AGCGACTACACTCTCTTCAATCTAATTCCCGTGGGCCTGCGTGTAGTGGCCATCCAAGGA
NM_004113.5         AGCGACTACACTCTCTTCAATCTAATTCCCGTGGGCCTGCGTGTAGTGGCCATCCAAGGA
XM_005247227.1      AGCGACTACACTCTCTTCAATCTAATTCCCGTGGGCCTGCGTGTAGTGGCCATCCAAGGA
NM_021032.4         AGCGACTACACTCTCTTCAATCTAATTCCCGTGGGCCTGCGTGTAGTGGCCATCCAAGGA
                    ************************************************************

HsCD00441911        GTGAAGGCTAGCCTCTATGTGGCCATGAATGGTGAAGGCTATCTCTACAGTTCAGATGTT
NM_004113.5         GTGAAGGCTAGCCTCTATGTGGCCATGAATGGTGAAGGCTATCTCTACAGTTCAGATGTT
XM_005247227.1      GTGAAGGCTAGCCTCTATGTGGCCATGAATGGTGAAGGCTATCTCTACAGTTCAGATGTT
NM_021032.4         GTGAAGGCTAGCCTCTATGTGGCCATGAATGGTGAAGGCTATCTCTACAGTTCAGATGTT
                    ************************************************************

HsCD00441911        TTCACTCCAGAATGCAAATTCAAGGAATCTGTGTTTGAAAACTACTATGTGATCTATTCT
NM_004113.5         TTCACTCCAGAATGCAAATTCAAGGAATCTGTGTTTGAAAACTACTATGTGATCTATTCT
XM_005247227.1      TTCACTCCAGAATGCAAATTCAAGGAATCTGTGTTTGAAAACTACTATGTGATCTATTCT
NM_021032.4         TTCACTCCAGAATGCAAATTCAAGGAATCTGTGTTTGAAAACTACTATGTGATCTATTCT
                    ************************************************************

HsCD00441911        TCCACACTGTACCGCCAGCAAGAATCAGGCCGAGCTTGGTTTCTGGGACTCAATAAAGAA
NM_004113.5         TCCACACTGTACCGCCAGCAAGAATCAGGCCGAGCTTGGTTTCTGGGACTCAATAAAGAA
XM_005247227.1      TCCACACTGTACCGCCAGCAAGAATCAGGCCGAGCTTGGTTTCTGGGACTCAATAAAGAA
NM_021032.4         TCCACACTGTACCGCCAGCAAGAATCAGGCCGAGCTTGGTTTCTGGGACTCAATAAAGAA
                    ************************************************************

HsCD00441911        GGTCAAATTATGAAGGGGAACAGAGTGAAGAAAACCAAGCCCTCATCACATTTTGTACCG
NM_004113.5         GGTCAAATTATGAAGGGGAACAGAGTGAAGAAAACCAAGCCCTCATCACATTTTGTACCG
XM_005247227.1      GGTCAAATTATGAAGGGGAACAGAGTGAAGAAAACCAAGCCCTCATCACATTTTGTACCG
NM_021032.4         GGTCAAATTATGAAGGGGAACAGAGTGAAGAAAACCAAGCCCTCATCACATTTTGTACCG
                    ************************************************************

HsCD00441911        AAACCTATTGAAGTGTGTATGTACAGAGAACAATCGCTACATGAAATTGGAGAAAAACAA
NM_004113.5         AAACCTATTGAAGTGTGTATGTACAGAGAACCATCGCTACATGAAATTGGAGAAAAACAA
XM_005247227.1      AAACCTATTGAAGTGTGTATGTACAGAGAACCATCGCTACATGAAATTGGAGAAAAACAA
NM_021032.4         AAACCTATTGAAGTGTGTATGTACAGAGAACCATCGCTACATGAAATTGGAGAAAAACAA
                    *******************************.****************************

HsCD00441911        GGGCGTTCAAGGAAAAGTTCTGGAACACCAACCATGAATGGAGGCAAAGTTGTGAATCAA
NM_004113.5         GGGCGTTCAAGGAAAAGTTCTGGAACACCAACCATGAATGGAGGCAAAGTTGTGAATCAA
XM_005247227.1      GGGCGTTCAAGGAAAAGTTCTGGAACACCAACCATGAATGGAGGCAAAGTTGTGAATCAA
NM_021032.4         GGGCGTTCAAGGAAAAGTTCTGGAACACCAACCATGAATGGAGGCAAAGTTGTGAATCAA
                    ************************************************************

HsCD00441911        GATTCAACA---
NM_004113.5         GATTCAACATAG
XM_005247227.1      GATTCAACATAG
NM_021032.4         GATTCAACATAG
                    *********   

Pathways

Panther : FGF signaling pathway
WikiPathway : Regulation of Actin Cytoskeleton

Clone

Original Clone ID : ccsbBroad304_06210

Organism


TAX_ID : 9606
Species Specific ID: 2257

Genome

Chromosome : 3
Map Location : 3q28
Ensembl : ENSG00000114279

Reagents

Labome : FGF12-antibody