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RNASE11 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  RNASE11
Gene Name:  ribonuclease, RNase A family, 11 (non-active)
Sequence             
Original Clone ID: ccsbBroad304_04765
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00441981

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 598nts         Open reading frame : 1 to 598
Reference Sequence Alignment

ATGGAGACCTTTCCTCTGCTGCTGCTCAGCCTGGGCCTGGTTCTTGCAGAAGCATCAGAA
AGCACAATGAAGATAATTAAAGAAGAATTTACAGACGAAGAGATGCAATATGACATGGCA
AAAAGTGGCCAAGAAAAACAGACCATTGAGATATTAATGAACCCGATCCTGTTAGTTAAA
AATACCAGCCTCAGCATGTCCAAGGATGATATGTCTTCCACATTACTGACATTCAGAAGT
TTACATTATAATGACCCCAAGGGAAACAGTTCGGGTAATGACAAAGAGTGTTGCAATGAC
ATGACAGTCTGGAGAAAAGTTTCAGAAGCAAACGGATCGTGCAAGTGGAGCAATAACTTC
ATCCGCAGCTCCACAGAAGTGATGCGCAGGGTCCACAGGGCCCCCAGCTGCAAGTTTGTA
CAGAATCCTGGCATAAGCTGCTGTGAGAGCCTAGAACTGGAAAATACAGTGTGCCAGTTC
ACTACAGGCAAACAATTCCCCAGGTGCCAATACCATAGTGTTACCTCATTAGAGAAGATA
TTGACAGTGCTGACAGGTCATTCTCTGATGAGCTGGTTAGTTTGTGGCTCTAAGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
                                

5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : RNASE11
Symbol Nomenclature : RNASE11
SYNONYM : C14orf6; HEL-S-84p; RAJ1
Designation : RNase 11|epididymis secretory sperm binding protein Li 84p|probable ribonuclease 11|ribonuclease 11|ribonuclease A J1
Full Nomenclature : ribonuclease, RNase A family, 11 (non-active)
GENEID : 122651
Locus Tag : UNQ5832/PRO19669
HGNC : 19269
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00441981      ATGGAGACCTTTCCTCTGCTGCTGCTCAGCCTGGGCCTGGTTCTTGCAGAAGCATCAGAA
NM_145250.3       ATGGAGACCTTTCCTCTGCTGCTGCTCAGCCTGGGCCTGGTTCTTGCAGAAGCATCAGAA
                  ************************************************************

HsCD00441981      AGCACAATGAAGATAATTAAAGAAGAATTTACAGACGAAGAGATGCAATATGACATGGCA
NM_145250.3       AGCACAATGAAGATAATTAAAGAAGAATTTACAGACGAAGAGATGCAATATGACATGGCA
                  ************************************************************

HsCD00441981      AAAAGTGGCCAAGAAAAACAGACCATTGAGATATTAATGAACCCGATCCTGTTAGTTAAA
NM_145250.3       AAAAGTGGCCAAGAAAAACAGACCATTGAGATATTAATGAACCCGATCCTGTTAGTTAAA
                  ************************************************************

HsCD00441981      AATACCAGCCTCAGCATGTCCAAGGATGATATGTCTTCCACATTACTGACATTCAGAAGT
NM_145250.3       AATACCAGCCTCAGCATGTCCAAGGATGATATGTCTTCCACATTACTGACATTCAGAAGT
                  ************************************************************

HsCD00441981      TTACATTATAATGACCCCAAGGGAAACAGTTCGGGTAATGACAAAGAGTGTTGCAATGAC
NM_145250.3       TTACATTATAATGACCCCAAGGGAAACAGTTCGGGTAATGACAAAGAGTGTTGCAATGAC
                  ************************************************************

HsCD00441981      ATGACAGTCTGGAGAAAAGTTTCAGAAGCAAACGGATCGTGCAAGTGGAGCAATAACTTC
NM_145250.3       ATGACAGTCTGGAGAAAAGTTTCAGAAGCAAACGGATCGTGCAAGTGGAGCAATAACTTC
                  ************************************************************

HsCD00441981      ATCCGCAGCTCCACAGAAGTGATGCGCAGGGTCCACAGGGCCCCCAGCTGCAAGTTTGTA
NM_145250.3       ATCCGCAGCTCCACAGAAGTGATGCGCAGGGTCCACAGGGCCCCCAGCTGCAAGTTTGTA
                  ************************************************************

HsCD00441981      CAGAATCCTGGCATAAGCTGCTGTGAGAGCCTAGAACTGGAAAATACAGTGTGCCAGTTC
NM_145250.3       CAGAATCCTGGCATAAGCTGCTGTGAGAGCCTAGAACTGGAAAATACAGTGTGCCAGTTC
                  ************************************************************

HsCD00441981      ACTACAGGCAAACAATTCCCCAGGTGCCAATACCATAGTGTTACCTCATTAGAGAAGATA
NM_145250.3       ACTACAGGCAAACAATTCCCCAGGTGCCAATACCATAGTGTTACCTCATTAGAGAAGATA
                  ************************************************************

HsCD00441981      TTGACAGTGCTGACAGGTCATTCTCTGATGAGCTGGTTAGTTTGTGGCTCTAAGTTG---
NM_145250.3       TTGACAGTGCTGACAGGTCATTCTCTGATGAGCTGGTTAGTTTGTGGCTCTAAGTTGTAA
                  *********************************************************   

Protein

SMART domain : RNAse_Pc : Pancreatic ribonuclease
HPRD : 15257

Clone

Original Clone ID : ccsbBroad304_04765

Organism


TAX_ID : 9606
Species Specific ID: 122651

Genome

Chromosome : 14
Map Location : 14q11.2
Ensembl : ENSG00000173464

Reagents

Labome : RNASE11-antibody