Title: Allosteric Inhibition of Epac: Computational Modeling and Experimental Validation to Identify Allosteric Sites and Inhibitors
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Special MTA: None
ATGCTCTACAAGAAATATCGACAGTATATGGCAGGACTTCTGGCTCCTCCTTATGGTGTT
ATGGAAACGGGCTCTAACAATGACAGGATTCCTGACAAGGAGAACACACCTCTCATTGAA
CCTCACGTTCCTCTTCGTCCTGCTAACACCATTACCAAGGTCCCTTCAGAGAAGATCCTC
AGAGCTGGAAAAATTTTACGAAATGCCATTCTCTCTCGAGCACCTCACATGATAAGAGAT
AGAAAATACCACCTAAAGACATACAGACAATGCTGTGTGGGAACTGAACTGGTGGACTGG
ATGATGCAGCAGACACCATGTGTTCACTCCCGGACTCAAGCTGTTGGCATGTGGCAAGTC
CTGTTAGAAGATGGTGTTCTCAACCACGTGGACCAGGAGCACCATTTCCAAGACAAATAT
TTATTCTATCGATTTCTGGATGATGAGCACGAGGATGCCCCTTTGCCTACTGAGGAGGAG
AAGAAGGAGTGTGATGAGGAGCTCCAGGACACCATGCTGCTGCTGTCACAGATGGGCCGC
GACGCCCACATGAGGATGATCCTTCGCAAACCACCTGGCCAGAGGACTGTGGATGACCTA
GAGATTATCTATGAGGAGCTTCTTCATATTAAAGCCTTATCCCATCTTTCTACCACAGTG
AAACGAGAGTTAGCAGGTGTTCTCATTTTTGAGTCTCACGCCAAAGGAGGGACTGTGTTG
TTTAACCAGGGGGAAGAAGGTACCTCCTGGTACATTATTCTAAAAGGATCAGTGAATGTA
GTCATTTACGGCAAGGGTGTGGTCTGCACCCTGCATGAAGGAGATGACTTCGGCAAGTTA
GCACTAGTGAATGATGCCCCACGAGCTGCCTCTATCGTCTTACGAGAAGATAACTGCCAT
TTCTTAAGAGTAGACAAGGAGGATTTCAACCGGATCCTAAGGGACGTGGAGGCGAATACA
GTCAGACTTAAAGAACATGACCAAGATGTCTTGGTGCTGGAGAAGGTCCCAGCAGGGAAC
AGAGCTTCTAATCAAGGAAACTCACAGCCTCAGCAAAAGTATACTGTGATGTCAGGAACA
CCTGAAAAAATTTTAGAGCGTTTTCTAGAAACAATACGCCTTGAGGCAACTTTAAATGAA
GCAACAGATTCTGTTTTAAATGACTTTATTATGATGCACTGTGTTTTTATGCCAAATACC
CAGCTTTGCCCGGCACTGGTGGCCCACTACCACGCACAGCCTTCACAAGGTACAGAACAG
GAGAAAATGGATTATGCCCTCAACAATAAGAGGCGAGTCATCCGCCTGGTTCTACAGTGG
GCTGCCATGTATGGAGACCTCCTGCAAGAGGATGACGTGTCTATGGCCTTCCTGGAGGAG
TTTTATGTATCTGTATCAGATGATGCCCGGATGATTGCTGCCCTCAAGGAGCAACTGCCA
GAGTTGGAGAAGATTGTCAAGCAAATCTCAGAAGATGCAAAGGCACCACAAAAGAAGCAC
AAGGTTATTTTGCAACAGTTCAATACGGGCGATGAGAGAGCCCAGAAGCGCCAGCCTATC
CGCGGCTCTGATGAAGTTCTGTTTAAGGTCTATTGCATGGACCACACCTACACAACCATT
CGGGTGCCAGTGGCCACTTCGGTGAAGGAAGTCATCAGTGCAGTTGCCGACAAGCTGGGC
TCCGGGGAGGGCCTGATCATAGTCAAGATGAGTTCCGGAGGAGAAAAGGTGGTGCTCAAA
CCTAATGATGTTTCAGTATTTACGACGCTCACCATTAATGGACGCCTGTTTGCTTGCCCG
CGAGAGCAATTCGATTCACTGACTCCATTACCAGAACAGGAAGGCCCAACTGTTGGAACA
GTGGGAACTTTTGAACTGATGAGCTCCAAAGATTTAGCATACCAGATGACAATTTATGAT
TGGGAACTCTTCAACTGCGTGCATGAGCTGGAGCTAATCTATCACACATTTGGAAGGCAT
AATTTTAAAAAGACCACAGCAAACTTGGATTTGTTCCTGAGGAGATTTAATGAAATTCAG
TTTTGGGTCGTCACTGAGATCTGCCTTTGTTCTCAGCTCAGCAAGCGTGTTCAGCTATTA
AAAAAATTTATTAAGATAGCAGCCCACTGTAAGGAGTATAAAAATCTGAATTCCTTTTTT
GCCATCGTCATGGGACTAAGTAACGTTGCTGTGAGCCGCTTGGCACTAACGTGGGAGAAA
CTGCCAAGCAAGTTCAAGAAGTTCTATGCGGAGTTTGAAAGTTTAATGGACACTTCAAGG
AACCACAGGGCCTACAGGCTGACAGTAGCTAAGCTGGAACCTCCTCTCATCCCCTTCATG
CCTTTGCTCATTAAAGATATGACATTTACTCATGAGGGGAACAAGACGTTCATTGACAAT
CTAGTAAACTTTGAAAAAATGCGCATGATTGCAAATACGGCCAGAACAGTGAGATACTAC
AGGAGCCAACCCTTCAATCCTGATGCAGCTCAAGCTAATAAGAACCATCAGGATGTCCGG
AGTTATGTACGGCAATTAAATGTGATTGACAACCAGAGAACTTTATCACAGATGTCACAC
AGATTAGAGCCTCGTCGACCA
MLYKKYRQYMAGLLAPPYGVMETGSNNDRIPDKENTPLIEPHVPLRPANTITKVPSEKIL RAGKILRNAILSRAPHMIRDRKYHLKTYRQCCVGTELVDWMMQQTPCVHSRTQAVGMWQV LLEDGVLNHVDQEHHFQDKYLFYRFLDDEHEDAPLPTEEEKKECDEELQDTMLLLSQMGR DAHMRMILRKPPGQRTVDDLEIIYEELLHIKALSHLSTTVKRELAGVLIFESHAKGGTVL FNQGEEGTSWYIILKGSVNVVIYGKGVVCTLHEGDDFGKLALVNDAPRAASIVLREDNCH FLRVDKEDFNRILRDVEANTVRLKEHDQDVLVLEKVPAGNRASNQGNSQPQQKYTVMSGT PEKILERFLETIRLEATLNEATDSVLNDFIMMHCVFMPNTQLCPALVAHYHAQPSQGTEQ EKMDYALNNKRRVIRLVLQWAAMYGDLLQEDDVSMAFLEEFYVSVSDDARMIAALKEQLP ELEKIVKQISEDAKAPQKKHKVILQQFNTGDERAQKRQPIRGSDEVLFKVYCMDHTYTTI RVPVATSVKEVISAVADKLGSGEGLIIVKMSSGGEKVVLKPNDVSVFTTLTINGRLFACP REQFDSLTPLPEQEGPTVGTVGTFELMSSKDLAYQMTIYDWELFNCVHELELIYHTFGRH NFKKTTANLDLFLRRFNEIQFWVVTEICLCSQLSKRVQLLKKFIKIAAHCKEYKNLNSFF AIVMGLSNVAVSRLALTWEKLPSKFKKFYAEFESLMDTSRNHRAYRLTVAKLEPPLIPFM PLLIKDMTFTHEGNKTFIDNLVNFEKMRMIANTARTVRYYRSQPFNPDAAQANKNHQDVR SYVRQLNVIDNQRTLSQMSHRLEPRRP
Coding Sequence Details
Recommended Growth Condition:
Host Type: mammalian Marker: blasticidin