DNASU Plasmid Repository • 480.965.5697 | Email

MPG (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  MPG
Gene Name:  N-methylpurine-DNA glycosylase
Sequence             
Original Clone ID: ccsbBroad304_01031
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00442379

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 880nts         Open reading frame : 1 to 880
Reference Sequence Alignment

ATGCCCGCGCGCAGCGGGGCCCAGTTTTGCCGACGGATGGGGCAAAAGAAGCAGCGACCA
GCTAGAGCAGGGCAGCCACACAGCTCGTCCGACGCAGCCCAGGCACCTGCAGAGCAGCCA
CACAGCTCGTCCGATGCAGCCCAGGCACCTTGCCCCAGGGAGCGCTGCTTGGGACCGCCC
ACCACTCCGGGCCCATACCGCAGCATCTATTTCTCAAGCCCAAAGGGCCACCTTACCCGA
CTGGGGTTGGAGTTCTTCGACCAGCCGGCAGTCCCCCTGGCCCGGGCATTTCTGGGACAG
GTCCTAGTCCGGCGACTTCCTAATGGCACAGAACTCCGAGGCCGCATCGTGGAGACCGAG
GCATACCTGGGGCCAGAGGATGAAGCCGCCCACTCAAGGGGTGGCCGGCAGACCCCCCGC
AACCGAGGCATGTTCATGAAGCCGGGGACCCTGTACGTGTACATCATTTACGGCATGTAC
TTCTGCATGAACATCTCCAGCCAGGGGGACGGGGCTTGCGTCTTGCTGCGAGCACTGGAG
CCCCTGGAAGGTCTGGAGACCATGCGTCAGCTTCGCAGCACCCTCCGGAAAGGCACCGCC
AGCCGTGTCCTCAAGGACCGCGAGCTCTGCAGTGGCCCCTCCAAGCTGTGCCAGGCCCTG
GCCATCAACAAGAGCTTTGACCAGAGGGACCTGGCACAGGATGAAGCTGTATGGCTGGAG
CGTGGTCCCCTGGAGCCCAGTGAGCCGGCTGTAGTGGCAGCAGCCCGGGTGGGCGTCGGC
CATGCAGGGGAGTGGGCCCGGAAACCCCTCCGCTTCTATGTCCGGGGCAGCCCCTGGGTC
AGTGTGGTCGACAGAGTGGCTGAGCAGGACACACAGGCC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : MPG
Symbol Nomenclature : MPG
SYNONYM : AAG; ADPG; APNG; CRA36.1; MDG; Mid1; PIG11; PIG16; anpg
Designation : 3' end of the Mid1 gene, localized 68 kb upstream the humanzeta globin gene on 16p|3-alkyladenine DNA glycosylase|3-methyladenine DNA glycosidase|CRA36.1 (3-methyl-adenine DNA glycosylase)|DNA-3-methyladenine glycosylase|N-methylpurine-DNA glycosylase, MPG|proliferation-inducing protein 11|proliferation-inducing protein 16
Full Nomenclature : N-methylpurine-DNA glycosylase
GENEID : 4350
Locus Tag : Z69720.1-002
GenBank Accession : DQ895912
HGNC : 7211
MIM : 156565
Vega : OTTHUMG00000047887
Target GenBank: DQ895912

 

Reference Sequence Alignment

  

HsCD00442379        ---------------ATGCCCGCGCGCAGCGGGGCCCAGTTTTGCCGACGGATGGGGCAA
NM_001015054.2      ---------------------------------------------------ATGGGGCAA
NM_001015052.2      ---------------ATGCCCGCGCGCAGCGGGGCCCAGTTTTGCCGACGGATGGGGCAA
NM_002434.3         ATGGTCACCCCCGCTTTGCAGATGAAGAAACCAAAGCAGTTTTGCCGACGGATGGGGCAA
                                                                       *********

HsCD00442379        AAGAAGCAGCGACCAGCTAGAGCAGGGCAGCCACACAGCTCGTCCGACGCAGCCCAGGCA
NM_001015054.2      AAGAAGCAGCGACCAGCTAGAGCAGGGCAGCCACACAGCTCGTCCGACGCAGCCCAGGCA
NM_001015052.2      AAGAAGCAGCGACCAGCTAGAGCAGGGCAGCCACACAGCTCGTCCGACGCAGCCCAGGCA
NM_002434.3         AAGAAGCAGCGACCAGCTAGAGCAGGGCAGCCACACAGCTCGTCCGACGCAGCCCAGGCA
                    ************************************************************

HsCD00442379        CCTGCAGAGCAGCCACACAGCTCGTCCGATGCAGCCCAGGCACCTTGCCCCAGGGAGCGC
NM_001015054.2      CCTGCAGAGCAGCCACACAGCTCGTCCGATGCAGCCCAGGCACCTTGCCCCAGGGAGCGC
NM_001015052.2      CCTGCAGAGCAGCCACACAGCTCGTCCGATGCAGCCCAGGCACCTTGCCCCAGGGAGCGC
NM_002434.3         CCTGCAGAGCAGCCACACAGCTCGTCCGATGCAGCCCAGGCACCTTGCCCCAGGGAGCGC
                    ************************************************************

HsCD00442379        TGCTTGGGACCGCCCACCACTCCGGGCCCATACCGCAGCATCTATTTCTCAAGCCCAAAG
NM_001015054.2      TGCTTGGGACCGCCCACCACTCCGGGCCCATACCGCAGCATCTATTTCTCAAGCCCAAAG
NM_001015052.2      TGCTTGGGACCGCCCACCACTCCGGGCCCATACCGCAGCATCTATTTCTCAAGCCCAAAG
NM_002434.3         TGCTTGGGACCGCCCACCACTCCGGGCCCATACCGCAGCATCTATTTCTCAAGCCCAAAG
                    ************************************************************

HsCD00442379        GGCCACCTTACCCGACTGGGGTTGGAGTTCTTCGACCAGCCGGCAGTCCCCCTGGCCCGG
NM_001015054.2      GGCCACCTTACCCGACTGGGGTTGGAGTTCTTCGACCAGCCGGCAGTCCCCCTGGCCCGG
NM_001015052.2      GGCCACCTTACCCGACTGGGGTTGGAGTTCTTCGACCAGCCGGCAGTCCCCCTGGCCCGG
NM_002434.3         GGCCACCTTACCCGACTGGGGTTGGAGTTCTTCGACCAGCCGGCAGTCCCCCTGGCCCGG
                    ************************************************************

HsCD00442379        GCATTTCTGGGACAGGTCCTAGTCCGGCGACTTCCTAATGGCACAGAACTCCGAGGCCGC
NM_001015054.2      GCATTTCTGGGACAGGTCCTAGTCCGGCGACTTCCTAATGGCACAGAACTCCGAGGCCGC
NM_001015052.2      GCATTTCTGGGACAGGTCCTAGTCCGGCGACTTCCTAATGGCACAGAACTCCGAGGCCGC
NM_002434.3         GCATTTCTGGGACAGGTCCTAGTCCGGCGACTTCCTAATGGCACAGAACTCCGAGGCCGC
                    ************************************************************

HsCD00442379        ATCGTGGAGACCGAGGCATACCTGGGGCCAGAGGATGAAGCCGCCCACTCAAGGGGTGGC
NM_001015054.2      ATCGTGGAGACCGAGGCATACCTGGGGCCAGAGGATGAAGCCGCCCACTCAAGGGGTGGC
NM_001015052.2      ATCGTGGAGACCGAGGCATACCTGGGGCCAGAGGATGAAGCCGCCCACTCAAGGGGTGGC
NM_002434.3         ATCGTGGAGACCGAGGCATACCTGGGGCCAGAGGATGAAGCCGCCCACTCAAGGGGTGGC
                    ************************************************************

HsCD00442379        CGGCAGACCCCCCGCAACCGAGGCATGTTCATGAAGCCGGGGACCCTGTACGTGTACATC
NM_001015054.2      CGGCAGACCCCCCGCAACCGAGGCATGTTCATGAAGCCGGGGACCCTGTACGTGTACATC
NM_001015052.2      CGGCAGACCCCCCGCAACCGAGGCATGTTCATGAAGCCGGGGACCCTGTACGTGTACATC
NM_002434.3         CGGCAGACCCCCCGCAACCGAGGCATGTTCATGAAGCCGGGGACCCTGTACGTGTACATC
                    ************************************************************

HsCD00442379        ATTTACGGCATGTACTTCTGCATGAACATCTCCAGCCAGGGGGACGGGGCTTGCGTCTTG
NM_001015054.2      ATTTACGGCATGTACTTCTGCATGAACATCTCCAGCCAGGGGGACGGGGCTTGCGTCTTG
NM_001015052.2      ATTTACGGCATGTACTTCTGCATGAACATCTCCAGCCAGGGGGACGGGGCTTGCGTCTTG
NM_002434.3         ATTTACGGCATGTACTTCTGCATGAACATCTCCAGCCAGGGGGACGGGGCTTGCGTCTTG
                    ************************************************************

HsCD00442379        CTGCGAGCACTGGAGCCCCTGGAAGGTCTGGAGACCATGCGTCAGCTTCGCAGCACCCTC
NM_001015054.2      CTGCGAGCACTGGAGCCCCTGGAAGGTCTGGAGACCATGCGTCAGCTTCGCAGCACCCTC
NM_001015052.2      CTGCGAGCACTGGAGCCCCTGGAAGGTCTGGAGACCATGCGTCAGCTTCGCAGCACCCTC
NM_002434.3         CTGCGAGCACTGGAGCCCCTGGAAGGTCTGGAGACCATGCGTCAGCTTCGCAGCACCCTC
                    ************************************************************

HsCD00442379        CGGAAAGGCACCGCCAGCCGTGTCCTCAAGGACCGCGAGCTCTGCAGTGGCCCCTCCAAG
NM_001015054.2      CGGAAAGGCACCGCCAGCCGTGTCCTCAAGGACCGCGAGCTCTGCAGTGGCCCCTCCAAG
NM_001015052.2      CGGAAAGGCACCGCCAGCCGTGTCCTCAAGGACCGCGAGCTCTGCAGTGGCCCCTCCAAG
NM_002434.3         CGGAAAGGCACCGCCAGCCGTGTCCTCAAGGACCGCGAGCTCTGCAGTGGCCCCTCCAAG
                    ************************************************************

HsCD00442379        CTGTGCCAGGCCCTGGCCATCAACAAGAGCTTTGACCAGAGGGACCTGGCACAGGATGAA
NM_001015054.2      CTGTGCCAGGCCCTGGCCATCAACAAGAGCTTTGACCAGAGGGACCTGGCACAGGATGAA
NM_001015052.2      CTGTGCCAGGCCCTGGCCATCAACAAGAGCTTTGACCAGAGGGACCTGGCACAGGATGAA
NM_002434.3         CTGTGCCAGGCCCTGGCCATCAACAAGAGCTTTGACCAGAGGGACCTGGCACAGGATGAA
                    ************************************************************

HsCD00442379        GCTGTATGGCTGGAGCGTGGTCCCCTGGAGCCCAGTGAGCCGGCTGTAGTGGCAGCAGCC
NM_001015054.2      GCTGTATGGCTGGAGCGTGGTCCCCTGGAGCCCAGTGAGCCGGCTGTAGTGGCAGCAGCC
NM_001015052.2      GCTGTATGGCTGGAGCGTGGTCCCCTGGAGCCCAGTGAGCCGGCTGTAGTGGCAGCAGCC
NM_002434.3         GCTGTATGGCTGGAGCGTGGTCCCCTGGAGCCCAGTGAGCCGGCTGTAGTGGCAGCAGCC
                    ************************************************************

HsCD00442379        CGGGTGGGCGTCGGCCATGCAGGGGAGTGGGCCCGGAAACCCCTCCGCTTCTATGTCCGG
NM_001015054.2      CGGGTGGGCGTCGGCCATGCAGGGGAGTGGGCCCGGAAACCCCTCCGCTTCTATGTCCGG
NM_001015052.2      CGGGTGGGCGTCGGCCATGCAGGGGAGTGGGCCCGGAAACCCCTCCGCTTCTATGTCCGG
NM_002434.3         CGGGTGGGCGTCGGCCATGCAGGGGAGTGGGCCCGGAAACCCCTCCGCTTCTATGTCCGG
                    ************************************************************

HsCD00442379        GGCAGCCCCTGGGTCAGTGTGGTCGACAGAGTGGCTGAGCAGGACACACAGGCC---
NM_001015054.2      GGCAGCCCCTGGGTCAGTGTGGTCGACAGAGTGGCTGAGCAGGACACACAGGCCTGA
NM_001015052.2      GGCAGCCCCTGGGTCAGTGTGGTCGACAGAGTGGCTGAGCAGGACACACAGGCCTGA
NM_002434.3         GGCAGCCCCTGGGTCAGTGTGGTCGACAGAGTGGCTGAGCAGGACACACAGGCCTGA
                    ******************************************************   

Pathways

NCI : Validated nuclear estrogen receptor alpha network
Reactome : Base Excision Repair
Reactome : Base-Excision Repair, AP Site Formation
Reactome : Base-free sugar-phosphate removal via the single-nucleotide replacement pathway
Reactome : Cleavage of the damaged purine
Reactome : DNA Repair
Reactome : Depurination
Reactome : Displacement of DNA glycosylase by APE1
Reactome : Recognition and association of DNA glycosylase with site containing an affected purine
Reactome : Removal of DNA patch containing abasic residue
Reactome : Resolution of AP sites via the multiple-nucleotide patch replacement pathway
Reactome : Resolution of AP sites via the single-nucleotide replacement pathway
Reactome : Resolution of Abasic Sites (AP sites)

Protein

HPRD : 06766

Clone

Original Clone ID : ccsbBroad304_01031

Organism


TAX_ID : 9606
Species Specific ID: 4350

Genome

Chromosome : 16
Map Location : 16p13.3
Ensembl : ENSG00000103152

Reagents

Labome : MPG-antibody