DNASU Plasmid Repository • 480.965.5697 | Email

UBOX5 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  UBOX5
Gene Name:  U-box domain containing 5
Sequence             
Original Clone ID: ccsbBroad304_02697
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00442462

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1624nts         Open reading frame : 1 to 1624
Reference Sequence Alignment

ATGGTAATAAATCTTTGCCTCCCACAGTTCAGACCAAGAATTCACTGCAACAAGATATCA
GCTGATGGTTACGAAGTAGAAAATCTCATCTCTGAAGATCTCACAAAGAGAAGTCATGGT
TTCAGGACAGAGTATTTCATTAAGCCACCAGTCTATGTGACAGTTTCATTTCCCTTTAAT
GTGGAAATCTGTAGGATCAACATAGACCTCACAGCTGGGGGAGGTCAGAACGTCACTGGC
CTGGAAATGTACACATCTGCCTCATCTAGCAGAGTGTCTTGGAATACGCCCCAGTGCCGG
ACCCTGGGCCCAGCTGAGCCATCTGTCCCAGACAAGGAGGCGTTCACCTTGGTAGGCAAA
GTCTTACTGAAAAACCAGAGCCAAGTGGTGTTTAGCCACAGGGGCTTCAAGGCCAGGCCC
CCTTTTGGCGCGATGGAAGCCACACTCCCCTCCCCTGCTGTTGTGGCCCAGGAGCTCTGG
AATAAAGGGGCTCTTTCCCTTAGCCACGTGGCCCACTTAAGGATCTGTATCACCCATGTG
ACAGGCGGCGGTATCCCTTGTATCAAGCGGTTGGAAGTGTGGGGTCAGCCGGCCAAGACC
TGCTCCCAGGAAGTGATAGACAGCATCCTGCTGGTCACCTCAGAGAACCTGCCTCAGGAT
GTGGCTCTGCAGGCTCCAGCCTTGCCCATGGAAAGTGACTGTGACCCTGGGGACCAGCCT
GAGAGCCAGCAGGCTCCCTCCAGCCTGCAGAAGCTGGCCGAGATCATTCAGGATGTGCCT
GAGGAGTTCCTGGATCCCATCACCCTGGAGATCATGCCTTGTCCCATGCTGCTGCCCTCA
GGCAAGGTCATCGACCAGAGCACACTGGAGAAGTGTAACCGCAGTGAAGCCACATGGGGC
CGAGTGCCCAGTGACCCTTTCACGGGGGTAGCTTTTACTCCGCACTCTCAGCCCCTGCCT
CACCCCTCCCTCAAGGCCCGGATTGACCATTTCCTGCTCCAGCACTCCATCCCTGGCTGC
CACCTGCTTGGGAGAGCACAGACGGCATTGGCAGTGATCCCTTCTTCCATTGTTCTGCCC
TCTCAGAAAAGGAAGATAGAGCAGGCTGAACATGTCCCAGACAGTAACTTTGGTGTAAAT
GCTTCCTGTTTTTCTGCCACAAGCCCTTTGGTCTTACCCACTACCTCAGAGCACACTGCT
AAGAAAATGAAAGCCACCAATGAGCCCAGCCTGACACATATGGACTGCTCGACAGGTCCA
CTGTCCCACGAGCAGAAGCTGTCACAAAGCTTGGAAATTGCCTTGGCATCCACCCTTGGC
TCTATGCCCTCCTTCACGGCACGGCTGACCAGGGGACAGCTCCAGCACCTTGGCACAAGA
GGGAGCAACACTTCCTGGAGGCCTGGCACCGGCTCGGAGCAGCCTGGGAGCATCCTGGGC
CCCGAATGTGCCTCCTGCAAAAGAGTATTTTCTCCCTACTTCAAAAAGGAGCCGGTGTAC
CAGCTGCCCTGCGGCCACCTCCTGTGCCGACCCTGCCTGGGTGAGAAGCAACGCTCCCTG
CCCATGACGTGCACAGCCTGCCAGCGGCCGGTTGCTAGCCAAGACGTGCTGCGGGTCCAC
TTC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : UBOX5
Symbol Nomenclature : UBOX5
SYNONYM : RNF37; UBCE7IP5; UIP5
Designation : RING finger protein 37|U-box domain-containing protein 5|ubiquitin conjugating enzyme 7 interacting protein 5|ubiquitin-conjugating enzyme 7-interacting protein 5
Full Nomenclature : U-box domain containing 5
GENEID : 22888
Locus Tag : RP5-1187M17.9
GenBank Accession : DQ895205
HGNC : 17777
Vega : OTTHUMG00000031731
Target GenBank: DQ895205

 

Reference Sequence Alignment

  

HsCD00442462        ATGGTAATAAATCTTTGCCTCCCACAGTTCAGACCAAGAATTCACTGCAACAAGATATCA
NM_001267584.1      ATGGTAATAAATCTTTGCCTCCCACAGTTCAGACCAAGAATTCACTGCAACAAGATATCA
NM_014948.3         ATGGTAATAAATCTTTGCCTCCCACAGTTCAGACCAAGAATTCACTGCAACAAGATATCA
                    ************************************************************

HsCD00442462        GCTGATGGTTACGAAGTAGAAAATCTCATCTCTGAAGATCTCACAAAGAGAAGTCATGGT
NM_001267584.1      GCTGATGGTTACGAAGTAGAAAATCTCATCTCTGAAGATCTCACAAAGAGAAGTCATGGT
NM_014948.3         GCTGATGGTTACGAAGTAGAAAATCTCATCTCTGAAGATCTCACAAAGAGAAGTCATGGT
                    ************************************************************

HsCD00442462        TTCAGGACAGAGTATTTCATTAAGCCACCAGTCTATGTGACAGTTTCATTTCCCTTTAAT
NM_001267584.1      TTCAGGACAGAGTATTTCATTAAGCCACCAGTCTATGTGACAGTTTCATTTCCCTTTAAT
NM_014948.3         TTCAGGACAGAGTATTTCATTAAGCCACCAGTCTATGTGACAGTTTCATTTCCCTTTAAT
                    ************************************************************

HsCD00442462        GTGGAAATCTGTAGGATCAACATAGACCTCACAGCTGGGGGAGGTCAGAACGTCACTGGC
NM_001267584.1      GTGGAAATCTGTAGGATCAACATAGACCTCACAGCTGGGGGAGGTCAGAACGTCACTGGC
NM_014948.3         GTGGAAATCTGTAGGATCAACATAGACCTCACAGCTGGGGGAGGTCAGAACGTCACTGGC
                    ************************************************************

HsCD00442462        CTGGAAATGTACACATCTGCCTCATCTAGCAGAGTGTCTTGGAATACGCCCCAGTGCCGG
NM_001267584.1      CTGGAAATGTACACATCTGCCTCATCTAGCAGAGTGTCTTGGAATACGCCCCAGTGCCGG
NM_014948.3         CTGGAAATGTACACATCTGCCTCATCTAGCAGAGTGTCTTGGAATACGCCCCAGTGCCGG
                    ************************************************************

HsCD00442462        ACCCTGGGCCCAGCTGAGCCATCTGTCCCAGACAAGGAGGCGTTCACCTTGGTAGGCAAA
NM_001267584.1      ACCCTGGGCCCAGCTGAGCCATCTGTCCCAGACAAGGAGGCGTTCACCTTGGTAGGCAAA
NM_014948.3         ACCCTGGGCCCAGCTGAGCCATCTGTCCCAGACAAGGAGGCGTTCACCTTGGTAGGCAAA
                    ************************************************************

HsCD00442462        GTCTTACTGAAAAACCAGAGCCAAGTGGTGTTTAGCCACAGGGGCTTCAAGGCCAGGCCC
NM_001267584.1      GTCTTACTGAAAAACCAGAGCCAAGTGGTGTTTAGCCACAGGGGCTTCAAGGCCAGGCCC
NM_014948.3         GTCTTACTGAAAAACCAGAGCCAAGTGGTGTTTAGCCACAGGGGCTTCAAGGCCAGGCCC
                    ************************************************************

HsCD00442462        CCTTTTGGCGCGATGGAAGCCACACTCCCCTCCCCTGCTGTTGTGGCCCAGGAGCTCTGG
NM_001267584.1      CCTTTTGGCGCGATGGAAGCCACACTCCCCTCCCCTGCTGTTGTGGCCCAGGAGCTCTGG
NM_014948.3         CCTTTTGGCGCGATGGAAGCCACACTCCCCTCCCCTGCTGTTGTGGCCCAGGAGCTCTGG
                    ************************************************************

HsCD00442462        AATAAAGGGGCTCTTTCCCTTAGCCACGTGGCCCACTTAAGGATCTGTATCACCCATGTG
NM_001267584.1      AATAAAGGGGCTCTTTCCCTTAGCCACGTGGCCCACTTAAGGATCTGTATCACCCATGTG
NM_014948.3         AATAAAGGGGCTCTTTCCCTTAGCCACGTGGCCCACTTAAGGATCTGTATCACCCATGTG
                    ************************************************************

HsCD00442462        ACAGGCGGCGGTATCCCTTGTATCAAGCGGTTGGAAGTGTGGGGTCAGCCGGCCAAGACC
NM_001267584.1      ACAGGCGGCGGTATCCCTTGTATCAAGCGGTTGGAAGTGTGGGGTCAGCCGGCCAAGACC
NM_014948.3         ACAGGCGGCGGTATCCCTTGTATCAAGCGGTTGGAAGTGTGGGGTCAGCCGGCCAAGACC
                    ************************************************************

HsCD00442462        TGCTCCCAGGAAGTGATAGACAGCATCCTGCTGGTCACCTCAGAGAACCTGCCTCAGGAT
NM_001267584.1      TGCTCCCAGGAAGTGATAGACAGCATCCTGCTGGTCACCTCAGAGAACCTGCCTCAGGAT
NM_014948.3         TGCTCCCAGGAAGTGATAGACAGCATCCTGCTGGTCACCTCAGAGAACCTGCCTCAGGAT
                    ************************************************************

HsCD00442462        GTGGCTCTGCAGGCTCCAGCCTTGCCCATGGAAAGTGACTGTGACCCTGGGGACCAGCCT
NM_001267584.1      GTGGCTCTGCAGGCTCCAGCCTTGCCCATGGAAAGTGACTGTGACCCTGGGGACCAGCCT
NM_014948.3         GTGGCTCTGCAGGCTCCAGCCTTGCCCATGGAAAGTGACTGTGACCCTGGGGACCAGCCT
                    ************************************************************

HsCD00442462        GAGAGCCAGCAGGCTCCCTCCAGCCTGCAGAAGCTGGCCGAGATCATTCAGGATGTGCCT
NM_001267584.1      GAGAGCCAGCAGGCTCCCTCCAGCCTGCAGAAGCTGGCCGAGATCATTCAGGATGTGCCT
NM_014948.3         GAGAGCCAGCAGGCTCCCTCCAGCCTGCAGAAGCTGGCCGAGATCATTCAGGATGTGCCT
                    ************************************************************

HsCD00442462        GAGGAGTTCCTGGATCCCATCACCCTGGAGATCATGCCTTGTCCCATGCTGCTGCCCTCA
NM_001267584.1      GAGGAGTTCCTGGATCCCATCACCCTGGAGATCATGCCTTGTCCCATGCTGCTGCCCTCA
NM_014948.3         GAGGAGTTCCTGGATCCCATCACCCTGGAGATCATGCCTTGTCCCATGCTGCTGCCCTCA
                    ************************************************************

HsCD00442462        GGCAAGGTCATCGACCAGAGCACACTGGAGAAGTGTAACCGCAGTGAAGCCACATGGGGC
NM_001267584.1      GGCAAGGTCATCGACCAGAGCACACTGGAGAAGTGTAACCGCAGTGAAGCCACATGGGGC
NM_014948.3         GGCAAGGTCATCGACCAGAGCACACTGGAGAAGTGTAACCGCAGTGAAGCCACATGGGGC
                    ************************************************************

HsCD00442462        CGAGTGCCCAGTGACCCTTTCACGGGGGTAGCTTTTACTCCGCACTCTCAGCCCCTGCCT
NM_001267584.1      CGAGTGCCCAGTGACCCTTTCACGGGGGTAGCTTTTACTCCGCACTCTCAGCCCCTGCCT
NM_014948.3         CGAGTGCCCAGTGACCCTTTCACGGGGGTAGCTTTTACTCCGCACTCTCAGCCCCTGCCT
                    ************************************************************

HsCD00442462        CACCCCTCCCTCAAGGCCCGGATTGACCATTTCCTGCTCCAGCACTCCATCCCTGGCTGC
NM_001267584.1      CACCCCTCCCTCAAGGCCCGGATTGACCATTTCCTGCTCCAGCACTCCATCCCTGGCTGC
NM_014948.3         CACCCCTCCCTCAAGGCCCGGATTGACCATTTCCTGCTCCAGCACTCCATCCCTGGCTGC
                    ************************************************************

HsCD00442462        CACCTGCTTGGGAGAGCACAGACGGCATTGGCAGTGATCCCTTCTTCCATTGTTCTGCCC
NM_001267584.1      CACCTGCTTGGGAGAGCACAGACGGCATTGGCAGTGATCCCTTCTTCCATTGTTCTGCCC
NM_014948.3         CACCTGCTTGGGAGAGCACAGACGGCATTGGCAGTGATCCCTTCTTCCATTGTTCTGCCC
                    ************************************************************

HsCD00442462        TCTCAGAAAAGGAAGATAGAGCAGGCTGAACATGTCCCAGACAGTAACTTTGGTGTAAAT
NM_001267584.1      TCTCAGAAAAGGAAGATAGAGCAGGCTGAACATGTCCCAGACAGTAACTTTGGTGTAAAT
NM_014948.3         TCTCAGAAAAGGAAGATAGAGCAGGCTGAACATGTCCCAGACAGTAACTTTGGTGTAAAT
                    ************************************************************

HsCD00442462        GCTTCCTGTTTTTCTGCCACAAGCCCTTTGGTCTTACCCACTACCTCAGAGCACACTGCT
NM_001267584.1      GCTTCCTGTTTTTCTGCCACAAGCCCTTTGGTCTTACCCACTACCTCAGAGCACACTGCT
NM_014948.3         GCTTCCTGTTTTTCTGCCACAAGCCCTTTGGTCTTACCCACTACCTCAGAGCACACTGCT
                    ************************************************************

HsCD00442462        AAGAAAATGAAAGCCACCAATGAGCCCAGCCTGACACATATGGACTGCTCGACAGGTCCA
NM_001267584.1      AAGAAAATGAAAGCCACCAATGAGCCCAGCCTGACACATATGGACTGCTCGACAGGTCCA
NM_014948.3         AAGAAAATGAAAGCCACCAATGAGCCCAGCCTGACACATATGGACTGCTCGACAGGTCCA
                    ************************************************************

HsCD00442462        CTGTCCCACGAGCAGAAGCTGTCACAAAGCTTGGAAATTGCCTTGGCATCCACCCTTGGC
NM_001267584.1      CTGTCCCACGAGCAGAAGCTGTCACAAAGCTTGGAAATTGCCTTGGCATCCACCCTTGGC
NM_014948.3         CTGTCCCACGAGCAGAAGCTGTCACAAAGCTTGGAAATTGCCTTGGCATCCACCCTTGGC
                    ************************************************************

HsCD00442462        TCTATGCCCTCCTTCACGGCACGGCTGACCAGGGGACAGCTCCAGCACCTTGGCACAAGA
NM_001267584.1      TCTATGCCCTCCTTCACGGCACGGCTGACCAGGGGACAGCTCCAGCACCTTGGCACAAGA
NM_014948.3         TCTATGCCCTCCTTCACGGCACGGCTGACCAGGGGACAGCTCCAGCACCTTGGCACAAGA
                    ************************************************************

HsCD00442462        GGGAGCAACACTTCCTGGAGGCCTGGCACCGGCTCGGAGCAGCCTGGGAGCATCCTGGGC
NM_001267584.1      GGGAGCAACACTTCCTGGAGGCCTGGCACCGGCTCG-----GCCTGGGAGCATCCTGGGC
NM_014948.3         GGGAGCAACACTTCCTGGAGGCCTGGCACCGGCTCGGAGCAGCCTGGGAGCATCCTGGGC
                    ************************************     *******************

HsCD00442462        CCCGAATGTGCCTCCTGCAAAAGAGTATTTTCTCCCTACTTCAAAAAGGAGCCGGTGTAC
NM_001267584.1      CCCGAATGTGCCTCCTGCAAAAGAGTATTTTCTCCCTACTTCAAAAAGGAGCCGGTGTAC
NM_014948.3         CCCGAATGTGCCTCCTGCAAAAGAGTATTTTCTCCCTACTTCAAAAAGGAGCCGGTGTAC
                    ************************************************************

HsCD00442462        CAGCTGCCCTGCGGCCACCTCCTGTGCCGACCCTGCCTGGGTGAGAAGCAACGCTCCCTG
NM_001267584.1      CAGCTGCCCTGCGGCCACCTCCTGTGCCGACCCTGCCTGGGTGA----------------
NM_014948.3         CAGCTGCCCTGCGGCCACCTCCTGTGCCGACCCTGCCTGGGTGAGAAGCAACGCTCCCTG
                    ********************************************                

HsCD00442462        CCCATGACGTGCACAGCCTGCCAGCGGCCGGTTGCTAGCCAAGACGTGCTGCGGGTCCAC
NM_001267584.1      ------------------------------------------------------------
NM_014948.3         CCCATGACGTGCACAGCCTGCCAGCGGCCGGTTGCTAGCCAAGACGTGCTGCGGGTCCAC
                                                                                

HsCD00442462        TTC---
NM_001267584.1      ------
NM_014948.3         TTCTGA
                          

Pathways

Reactome : Adaptive Immune System
Reactome : Antigen processing: Ubiquitination & Proteasome degradation
Reactome : Class I MHC mediated antigen processing & presentation
Reactome : Immune System

Protein

SMART domain : Ubox : Modified RING finger domain
HPRD : 15605

Clone

Original Clone ID : ccsbBroad304_02697

Organism


TAX_ID : 9606
Species Specific ID: 22888

Genome

Chromosome : 20
Map Location : 20p13
Ensembl : ENSG00000185019

Reagents

Labome : UBOX5-antibody