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BPI (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  BPI
Gene Name:  bactericidal/permeability-increasing protein
Sequence             
Original Clone ID: ccsbBroad304_05903
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00442588

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1462nts         Open reading frame : 1 to 1462
Reference Sequence Alignment

ATGAGAGAGAACTTGGCCAGGGGCCCTTGCAACGCGCCGAGATGGGCGTCCCTGATGGTG
CTGGTCGCCATAGGCACCGCCGTGACAGCGGCCGTCAACCCTGGCGTCGTGGTCAGGATC
TCCCAGAAGGGCCTGGACTACGCCAGCCAGCAGGGGACGGCCGCTCTGCAGAAGGAGCTG
AAGAGGATCAAGATTCCTGACTACTCAGACAGCTTTAAGATCAAGCATCTTGGGAAGGGG
CATTATAGCTTCTACAGCATGGACATCCGTGAATTCCAGCTTCCCAGTTCCCAGATAAGC
ATGGTGCCCAATGTGGGCCTTAAGTTCTCCATCAGCAACGCCAATATCAAGATCAGCGGG
AAATGGAAGGCACAAAAGAGATTCTTAAAAATGAGCGGCAATTTTGACCTGAGCATAGAA
GGCATGTCCATTTCGGCTGATCTGAAGCTGGGCAGTAACCCCACGTCAGGCAAGCCCACC
ATCACCTGCTCCAGCTGCAGCAGCCACATCAACAGTGTCCACGTGCACATCTCAAAGAGC
AAAGTGGGGTGGCTGATCCAACTCTTCCACAAAAAAATTGAGTCTGCGCTTCGAAACAAG
ATGAACAGCCAGGTCTGCGAGAAAGTGACCAATTCTGTATCCTCCAAGCTGCAACCTTAT
TTCCAGACTCTGCCAGTAATGACCAAAATAGATTCTGTGGCTGGAATCAACTATGGTCTG
GTGGCACCTCCAGCAACCACGGCTGAGACCCTGGATGTACAGATGAAGGGGGAGTTTTAC
AGTGAGAACCACCACAATCCACCTCCCTTTGCTCCACCAGTGATGGAGTTTCCCGCTGCC
CATGACCGCATGGTATACCTGGGCCTCTCAGACTACTTCTTCAACACAGCCGGGCTTGTA
TACCAAGAGGCTGGGGTCTTGAAGATGACCCTTAGAGATGACATGATTCCAAAGGAGTCC
AAATTTCGACTGACAACCAAGTTCTTTGGAACCTTCCTACCTGAGGTGGCCAAGAAGTTT
CCCAACATGAAGATACAGATCCATGTCTCAGCCTCCACCCCGCCACACCTGTCTGTGCAG
CCCACCGGCCTTACCTTCTACCCTGCCGTGGATGTCCAGGCCTTTGCCGTCCTCCCCAAC
TCCTCCCTGGCTTCCCTCTTCCTGATTGGCATGCACACAACTGGTTCCATGGAGGTCAGC
GCCGAGTCCAACAGGCTTGTTGGAGAGCTCAAGCTGGATAGGCTGCTCCTGGAACTGAAG
CACTCAAATATTGGCCCCTTCCCGGTTGAATTGCTGCAGGATATCATGAACTACATTGTA
CCCATTCTTGTGCTGCCCAGGGTTAACGAGAAACTACAGAAAGGCTTCCCTCTCCCGACG
CCGGCCAGAGTCCAGCTCTACAACGTAGTGCTTCAGCCTCACCAGAACTTCCTGCTGTTC
GGTGCAGACGTTGTCTATAAA


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : BPI
Symbol Nomenclature : BPI
SYNONYM : BPIFD1; rBPI
Designation : BPI fold containing family D, member 1|CAP 57|bactericidal permeability-increasing protein|recombinant BPI holoprotein, rBPI
Full Nomenclature : bactericidal/permeability-increasing protein
GENEID : 671
Locus Tag : RP13-218F6_A.1
HGNC : 1095
MIM : 109195
Vega : OTTHUMG00000032441
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00442588        ATGAGAGAGAACTTGGCCAGGGGCCCTTGCAACGCGCCGAGATGGGCGTCCCTGATGGTG
NM_001725.2         ATGAGAGAGAACATGGCCAGGGGCCCTTGCAACGCGCCGAGATGGGCGTCCCTGATGGTG
XM_005260522.1      ATGAGAGAGAACATGGCCAGGGGCCCTTGCAACGCGCCGAGATGGGCGTCCCTGATGGTG
                    ************:***********************************************

HsCD00442588        CTGGTCGCCATAGGCACCGCCGTGACAGCGGCCGTCAACCCTGGCGTCGTGGTCAGGATC
NM_001725.2         CTGGTCGCCATAGGCACCGCCGTGACAGCGGCCGTCAACCCTGGCGTCGTGGTCAGGATC
XM_005260522.1      CTGGTCGCCATAGGCACCGCCGTGACAGCGGCCGTCAACCCTGGCGTCGTGGTCAGGATC
                    ************************************************************

HsCD00442588        TCCCAGAAGGGCCTGGACTACGCCAGCCAGCAGGGGACGGCCGCTCTGCAGAAGGAGCTG
NM_001725.2         TCCCAGAAGGGCCTGGACTACGCCAGCCAGCAGGGGACGGCCGCTCTGCAGAAGGAGCTG
XM_005260522.1      TCCCAGAAGGGCCTGGACTACGCCAGCCAGCAGGGGACGGCCGCTCTGCAGAAGGAGCTG
                    ************************************************************

HsCD00442588        AAGAGGATCAAGATTCCTGACTACTCAGACAGCTTTAAGATCAAGCATCTTGGGAAGGGG
NM_001725.2         AAGAGGATCAAGATTCCTGACTACTCAGACAGCTTTAAGATCAAGCATCTTGGGAAGGGG
XM_005260522.1      AAGAGGATCAAGATTCCTGACTACTCAGACAGCTTTAAGATCAAGCATCTTGGGAAGGGG
                    ************************************************************

HsCD00442588        CATTATAGCTTCTACAGCATGGACATCCGTGAATTCCAGCTTCCCAGTTCCCAGATAAGC
NM_001725.2         CATTATAGCTTCTACAGCATGGACATCCGTGAATTCCAGCTTCCCAGTTCCCAGATAAGC
XM_005260522.1      CATTATAGCTTCTACAGCATGGACATCCGTGAATTCCAGCTTCCCAGTTCCCAGATAAGC
                    ************************************************************

HsCD00442588        ATGGTGCCCAATGTGGGCCTTAAGTTCTCCATCAGCAACGCCAATATCAAGATCAGCGGG
NM_001725.2         ATGGTGCCCAATGTGGGCCTTAAGTTCTCCATCAGCAACGCCAATATCAAGATCAGCGGG
XM_005260522.1      ATGGTGCCCAATGTGGGCCTTAAGTTCTCCATCAGCAACGCCAATATCAAGATCAGCGGG
                    ************************************************************

HsCD00442588        AAATGGAAGGCACAAAAGAGATTCTTAAAAATGAGCGGCAATTTTGACCTGAGCATAGAA
NM_001725.2         AAATGGAAGGCACAAAAGAGATTCTTAAAAATGAGCGGCAATTTTGACCTGAGCATAGAA
XM_005260522.1      AAATGGAAGGCACAAAAGAGATTCTTAAAAATGAGCGGCAATTTTGACCTGAGCATAGAA
                    ************************************************************

HsCD00442588        GGCATGTCCATTTCGGCTGATCTGAAGCTGGGCAGTAACCCCACGTCAGGCAAGCCCACC
NM_001725.2         GGCATGTCCATTTCGGCTGATCTGAAGCTGGGCAGTAACCCCACGTCAGGCAAGCCCACC
XM_005260522.1      GGCATGTCCATTTCGGCTGATCTGAAGCTGGGCAGTAACCCCACGTCAGGCAAGCCCACC
                    ************************************************************

HsCD00442588        ATCACCTGCTCCAGCTGCAGCAGCCACATCAACAGTGTCCACGTGCACATCTCAAAGAGC
NM_001725.2         ATCACCTGCTCCAGCTGCAGCAGCCACATCAACAGTGTCCACGTGCACATCTCAAAGAGC
XM_005260522.1      ATCACCTGCTCCAGCTGCAGCAGCCACATCAACAGTGTCCACGTGCACATCTCAAAGAGC
                    ************************************************************

HsCD00442588        AAAGTGGGGTGGCTGATCCAACTCTTCCACAAAAAAATTGAGTCTGCGCTTCGAAACAAG
NM_001725.2         AAAGTGGGGTGGCTGATCCAACTCTTCCACAAAAAAATTGAGTCTGCGCTTCGAAACAAG
XM_005260522.1      AAAGTGGGGTGGCTGATCCAACTCTTCCACAAAAAAATTGAGTCTGCGCTTCGAAACAAG
                    ************************************************************

HsCD00442588        ATGAACAGCCAGGTCTGCGAGAAAGTGACCAATTCTGTATCCTCCAAGCTGCAACCTTAT
NM_001725.2         ATGAACAGCCAGGTCTGCGAGAAAGTGACCAATTCTGTATCCTCCGAGCTGCAACCTTAT
XM_005260522.1      ATGAACAGCCAGGTCTGCGAGAAAGTGACCAATTCTGTATCCTCCGAGCTGCAACCTTAT
                    *********************************************.**************

HsCD00442588        TTCCAGACTCTGCCAGTAATGACCAAAATAGATTCTGTGGCTGGAATCAACTATGGTCTG
NM_001725.2         TTCCAGACTCTGCCAGTAATGACCAAAATAGATTCTGTGGCTGGAATCAACTATGGTCTG
XM_005260522.1      TTCCAGACTCTGCCAGTAATGACCAAAATAGATTCTGTGGCTGGAATCAACTATGGTCTG
                    ************************************************************

HsCD00442588        GTGGCACCTCCAGCAACCACGGCTGAGACCCTGGATGTACAGATGAAGGGGGAGTTTTAC
NM_001725.2         GTGGCACCTCCAGCAACCACGGCTGAGACCCTGGATGTACAGATGAAGGGGGAGTTTTAC
XM_005260522.1      GTGGCACCTCCAGCAACCACGGCTGAGACCCTGGATGTACAGATGAAGGGGGAGTTTTAC
                    ************************************************************

HsCD00442588        AGTGAGAACCACCACAATCCACCTCCCTTTGCTCCACCAGTGATGGAGTTTCCCGCTGCC
NM_001725.2         AGTGAGAACCACCACAATCCACCTCCCTTTGCTCCACCAGTGATGGAGTTTCCCGCTGCC
XM_005260522.1      AGTGAGAACCACCACAATCCACCTCCCTTTGCTCCACCAGTGATGGAGTTTCCCGCTGCC
                    ************************************************************

HsCD00442588        CATGACCGCATGGTATACCTGGGCCTCTCAGACTACTTCTTCAACACAGCCGGGCTTGTA
NM_001725.2         CATGACCGCATGGTATACCTGGGCCTCTCAGACTACTTCTTCAACACAGCCGGGCTTGTA
XM_005260522.1      CATGACCGCATGGTATACCTGGGCCTCTCAGACTACTTCTTCAACACAGCCGGGCTTGTA
                    ************************************************************

HsCD00442588        TACCAAGAGGCTGGGGTCTTGAAGATGACCCTTAGAGATGACATGATTCCAAAGGAGTCC
NM_001725.2         TACCAAGAGGCTGGGGTCTTGAAGATGACCCTTAGAGATGACATGATTCCAAAGGAGTCC
XM_005260522.1      TACCAAGAGGCTGGGGTCTTGAAGATGACCCTTAGAGATGACATGATTCCAAAGGAGTCC
                    ************************************************************

HsCD00442588        AAATTTCGACTGACAACCAAGTTCTTTGGAACCTTCCTACCTGAGGTGGCCAAGAAGTTT
NM_001725.2         AAATTTCGACTGACAACCAAGTTCTTTGGAACCTTCCTACCTGAGGTGGCCAAGAAGTTT
XM_005260522.1      AAATTTCGACTGACAACCAAGTTCTTTGGAACCTTCCTACCTGAGGTGGCCAAGAAGTTT
                    ************************************************************

HsCD00442588        CCCAACATGAAGATACAGATCCATGTCTCAGCCTCCACCCCGCCACACCTGTCTGTGCAG
NM_001725.2         CCCAACATGAAGATACAGATCCATGTCTCAGCCTCCACCCCGCCACACCTGTCTGTGCAG
XM_005260522.1      CCCAACATGAAGATACAGATCCATGTCTCAGCCTCCACCCCGCCACACCTGTCTGTGCAG
                    ************************************************************

HsCD00442588        CCCACCGGCCTTACCTTCTACCCTGCCGTGGATGTCCAGGCCTTTGCCGTCCTCCCCAAC
NM_001725.2         CCCACCGGCCTTACCTTCTACCCTGCCGTGGATGTCCAGGCCTTTGCCGTCCTCCCCAAC
XM_005260522.1      CCCACCGGCCTTACCTTCTACCCTGCCGTGGATGTCCAGGCCTTTGCCGTCCTCCCCAAC
                    ************************************************************

HsCD00442588        TCCTCCCTGGCTTCCCTCTTCCTGATTGGCATGCACACAACTGGTTCCATGGAGGTCAGC
NM_001725.2         TCCTCCCTGGCTTCCCTCTTCCTGATTGGCATGCACACAACTGGTTCCATGGAGGTCAGC
XM_005260522.1      TCCTCCCTGGCTTCCCTCTTCCTGATTG--------------------------------
                    ****************************                                

HsCD00442588        GCCGAGTCCAACAGGCTTGTTGGAGAGCTCAAGCTGGATAGGCTGCTCCTGGAACTGAAG
NM_001725.2         GCCGAGTCCAACAGGCTTGTTGGAGAGCTCAAGCTGGATAGGCTGCTCCTGGAACTGAAG
XM_005260522.1      ------------------------------------------------------------
                                                                                

HsCD00442588        CACTCAAATATTGGCCCCTTCCCGGTTGAATTGCTGCAGGATATCATGAACTACATTGTA
NM_001725.2         CACTCAAATATTGGCCCCTTCCCGGTTGAATTGCTGCAGGATATCATGAACTACATTGTA
XM_005260522.1      -------------------GCATGGTTGAATTGCTGCAGGATATCATGAACTACATTGTA
                                        *. *************************************

HsCD00442588        CCCATTCTTGTGCTGCCCAGGGTTAACGAGAAACTACAGAAAGGCTTCCCTCTCCCGACG
NM_001725.2         CCCATTCTTGTGCTGCCCAGGGTTAACGAGAAACTACAGAAAGGCTTCCCTCTCCCGACG
XM_005260522.1      CCCATTCTTGTGCTGCCCAGGGTTAACGAGAAACTACAGAAAGGCTTCCCTCTCCCGACG
                    ************************************************************

HsCD00442588        CCGGCCAGAGTCCAGCTCTACAACGTAGTGCTTCAGCCTCACCAGAACTTCCTGCTGTTC
NM_001725.2         CCGGCCAGAGTCCAGCTCTACAACGTAGTGCTTCAGCCTCACCAGAACTTCCTGCTGTTC
XM_005260522.1      CCGGCCAGAGTCCAGCTCTACAACGTAGTGCTTCAGCCTCACCAGAACTTCCTGCTGTTC
                    ************************************************************

HsCD00442588        GGTGCAGACGTTGTCTATAAA---
NM_001725.2         GGTGCAGACGTTGTCTATAAATGA
XM_005260522.1      GGTGCAGACGTTGTCTATAAATGA
                    *********************   

Clone

Original Clone ID : ccsbBroad304_05903

Organism


TAX_ID : 9606
Species Specific ID: 671

Genome

Chromosome : 20
Map Location : 20q11.23
Ensembl : ENSG00000101425