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AQP8 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  AQP8
Gene Name:  aquaporin 8
Sequence             
Original Clone ID: ccsbBroad304_10681
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00442752

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 766nts         Open reading frame : 1 to 766
Reference Sequence Alignment

ATGTGTGAGCCTGAATTTGGCAATGACAAGGCCAGGGAGCCGAGCGTGGGTGGCAGGTGG
CGAGTGTCCTGGTACGAACGGTTTGTGCAGCCATGTCTGGTCGAACTGCTGGGCTCTGCT
CTCTTCATCTTCATCGGGTGCCTGTCGGTCATTGAGAATGGGACGGACACTGGGCTGCTG
CAGCCGGCCCTGGCCCACGGGCTGGCTTTGGGGCTCGTGATTGCCACGCTGGGGAATATC
AGTGGTGGACACTTCAACCCTGCGGTGTCCCTGGCAGCCATGCTGATCGGAGGCCTCAAC
CTGGTGATGCTCCTCCCGTACTGGGTCTCACAGCTGCTCGGGGGGATGCTCGGGGCTGCC
TTGGCCAAGGCGGTGAGTCCTGAGGAGAGGTTCTGGAATGCATCTGGGGCGGCCTTTGTG
ACAGTCCAGGAGCAGGGGCAGGTGGCAGGGGCGTTGGTGGCAGAGATCATCCTGACGACG
CTGCTGGCCCTGGCTGTATGCATGGGTGCCATCAATGAGAAGACAAAGGGCCCTCTGGCC
CCGTTCTCCATCGGCTTTGCCGTCACCGTGGATATCCTGGCTGGGGGCCCTGTGTCTGGA
GGCTGCATGAATCCCGCCCGTGCTTTTGGACCTGCGGTGGTGGCCAACCACTGGAACTTC
CACTGGATCTACTGGCTGGGCCCACTCCTGGCTGGCCTGCTTGTTGGACTGCTCATTAGG
TGCTTCATTGGAGATGGGAAGACCCGCCTCATCCTGAAGGCTCGG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : AQP8
Symbol Nomenclature : AQP8
SYNONYM : AQP-8
Designation : aquaporin-8
Full Nomenclature : aquaporin 8
GENEID : 343
GenBank Accession : HQ448602
HGNC : 642
MIM : 603750
Vega : OTTHUMG00000097013
Target GenBank: HQ448602

 

Reference Sequence Alignment

  

HsCD00442752      ------------------ATGTGTGAGCCTGAATTTGGCAATGACAAGGCCAGGGAGCCG
NM_001169.2       ATGTCTGGAGAGATAGCCATGTGTGAGCCTGAATTTGGCAATGACAAGGCCAGGGAGCCG
                                    ******************************************

HsCD00442752      AGCGTGGGTGGCAGGTGGCGAGTGTCCTGGTACGAACGGTTTGTGCAGCCATGTCTGGTC
NM_001169.2       AGCGTGGGTGGCAGGTGGCGAGTGTCCTGGTACGAACGGTTTGTGCAGCCATGTCTGGTC
                  ************************************************************

HsCD00442752      GAACTGCTGGGCTCTGCTCTCTTCATCTTCATCGGGTGCCTGTCGGTCATTGAGAATGGG
NM_001169.2       GAACTGCTGGGCTCTGCTCTCTTCATCTTCATCGGGTGCCTGTCGGTCATTGAGAATGGG
                  ************************************************************

HsCD00442752      ACGGACACTGGGCTGCTGCAGCCGGCCCTGGCCCACGGGCTGGCTTTGGGGCTCGTGATT
NM_001169.2       ACGGACACTGGGCTGCTGCAGCCGGCCCTGGCCCACGGGCTGGCTTTGGGGCTCGTGATT
                  ************************************************************

HsCD00442752      GCCACGCTGGGGAATATCAGTGGTGGACACTTCAACCCTGCGGTGTCCCTGGCAGCCATG
NM_001169.2       GCCACGCTGGGGAATATCAGTGGTGGACACTTCAACCCTGCGGTGTCCCTGGCAGCCATG
                  ************************************************************

HsCD00442752      CTGATCGGAGGCCTCAACCTGGTGATGCTCCTCCCGTACTGGGTCTCACAGCTGCTCGGG
NM_001169.2       CTGATCGGAGGCCTCAACCTGGTGATGCTCCTCCCGTACTGGGTCTCACAGCTGCTCGGG
                  ************************************************************

HsCD00442752      GGGATGCTCGGGGCTGCCTTGGCCAAGGCGGTGAGTCCTGAGGAGAGGTTCTGGAATGCA
NM_001169.2       GGGATGCTCGGGGCTGCCTTGGCCAAGGCGGTGAGTCCTGAGGAGAGGTTCTGGAATGCA
                  ************************************************************

HsCD00442752      TCTGGGGCGGCCTTTGTGACAGTCCAGGAGCAGGGGCAGGTGGCAGGGGCGTTGGTGGCA
NM_001169.2       TCTGGGGCGGCCTTTGTGACAGTCCAGGAGCAGGGGCAGGTGGCAGGGGCGTTGGTGGCA
                  ************************************************************

HsCD00442752      GAGATCATCCTGACGACGCTGCTGGCCCTGGCTGTATGCATGGGTGCCATCAATGAGAAG
NM_001169.2       GAGATCATCCTGACGACGCTGCTGGCCCTGGCTGTATGCATGGGTGCCATCAATGAGAAG
                  ************************************************************

HsCD00442752      ACAAAGGGCCCTCTGGCCCCGTTCTCCATCGGCTTTGCCGTCACCGTGGATATCCTGGCT
NM_001169.2       ACAAAGGGCCCTCTGGCCCCGTTCTCCATCGGCTTTGCCGTCACCGTGGATATCCTGGCT
                  ************************************************************

HsCD00442752      GGGGGCCCTGTGTCTGGAGGCTGCATGAATCCCGCCCGTGCTTTTGGACCTGCGGTGGTG
NM_001169.2       GGGGGCCCTGTGTCTGGAGGCTGCATGAATCCCGCCCGTGCTTTTGGACCTGCGGTGGTG
                  ************************************************************

HsCD00442752      GCCAACCACTGGAACTTCCACTGGATCTACTGGCTGGGCCCACTCCTGGCTGGCCTGCTT
NM_001169.2       GCCAACCACTGGAACTTCCACTGGATCTACTGGCTGGGCCCACTCCTGGCTGGCCTGCTT
                  ************************************************************

HsCD00442752      GTTGGACTGCTCATTAGGTGCTTCATTGGAGATGGGAAGACCCGCCTCATCCTGAAGGCT
NM_001169.2       GTTGGACTGCTCATTAGGTGCTTCATTGGAGATGGGAAGACCCGCCTCATCCTGAAGGCT
                  ************************************************************

HsCD00442752      CGG---
NM_001169.2       CGGTGA
                  ***   

Pathways

Reactome : Aquaporin-mediated transport
Reactome : Cellular responses to stress
Reactome : Detoxification of Reactive Oxygen Species
Reactome : Passive transport by Aquaporins
Reactome : Transmembrane transport of small molecules

Protein

HPRD : 04778

Clone

Original Clone ID : ccsbBroad304_10681

Organism


TAX_ID : 9606
Species Specific ID: 343

Genome

Chromosome : 16
Map Location : 16p12
Ensembl : ENSG00000103375

Reagents

Labome : aquaporin-8-antibody