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RNASE9 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  RNASE9
Gene Name:  ribonuclease, RNase A family, 9 (non-active)
Sequence             
Original Clone ID: ccsbBroad304_10108
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00443087

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 616nts         Open reading frame : 1 to 616
Reference Sequence Alignment

ATGATGAGAACTCTCATCACCACACACCCACTGCCCCTGCTTCTATTGCCGCAGCAGCTG
CTGCAGCTGGTGCAGTTTCAAGAGGTGGATACAGATTTTGATTTCCCAGAAGAAGATAAA
AAAGAAGAATTTGAAGAGTGTTTGGAAAAATTTTTTAGTACAGGGCCCGCCAGACCACCT
ACCAAAGAAAAAGTCAAAAGACGTGTCCTTATTGAACCTGGAATGCCACTAAATCATATA
GAGTACTGTAACCATGAAATCATGGGAAAAAATGTTTACTACAAACACCGTTGGGTGGCA
GAACATTACTTCCTTCTTATGCAATATGACGAGCTCCAAAAAATCTGTTACAACAGATTT
GTGCCATGTAAGAATGGAATTAGGAAATGTAACAGGAGCAAAGGTCTTGTAGAAGGAGTG
TATTGTAATTTAACAGAAGCATCTGAAATACCAGCGTGTAAATACGAATCACTTTATAGG
AAGGGCTACGTCCTTATCACTTGTTCATGGCAAAATGAAATGCAAAAACGTATTCCTCAT
ACTATAAATGATCTCGTGGAGCCACCTGAACACAGAAGTTTCCTCAGTGAGGATGGTGTC
TTTGTCATACCGCCC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : RNASE9
Symbol Nomenclature : RNASE9
SYNONYM : HEL128; RAK1; h461
Designation : inactive ribonuclease-like protein 9|ribonuclear enzyme|ribonuclease A K1
Full Nomenclature : ribonuclease, RNase A family, 9 (non-active)
GENEID : 390443
GenBank Accession : HQ258747
HGNC : 20673
MIM : 614014
Vega : OTTHUMG00000170989
Target GenBank: HQ258747

 

Reference Sequence Alignment

  

HsCD00443087        ---------------ATGATGAGAACTCTCATCACCACACACCCACTGCCCCTGCTTCTA
NM_001110359.1      ATGTCTGCAGGAAAAATGATGAGAACTCTCATCACCACACACCCACTGCCCCTGCTTCTA
NM_001289110.1      ---------------ATGATGAGAACTCTCATCACCACACACCCACTGCCCCTGCTTCTA
NM_001110356.1      ---------------ATGATGAGAACTCTCATCACCACACACCCACTGCCCCTGCTTCTA
NM_001001673.3      ---------------ATGATGAGAACTCTCATCACCACACACCCACTGCCCCTGCTTCTA
NM_001110361.1      ATGTCTGCAGGAAAAATGATGAGAACTCTCATCACCACACACCCACTGCCCCTGCTTCTA
NM_001110357.1      ---------------ATGATGAGAACTCTCATCACCACACACCCACTGCCCCTGCTTCTA
NM_001110360.1      ATGTCTGCAGGAAAAATGATGAGAACTCTCATCACCACACACCCACTGCCCCTGCTTCTA
NM_001110358.1      ATGTCTGCAGGAAAAATGATGAGAACTCTCATCACCACACACCCACTGCCCCTGCTTCTA
                                   *********************************************

HsCD00443087        TTGCCGCAGCAGCTGCTGCAGCTGGTGCAGTTTCAAGAGGTGGATACAGATTTTGATTTC
NM_001110359.1      TTGCCGCAGCAGCTGCTGCAGCTGGTGCAGTTTCAAGAGGTGGATACAGATTTTGATTTC
NM_001289110.1      TTGCCGCAGCAGCTGCTGCAGCTGGTGCAGTTTCAAGAGGTGGATACAGATTTTGATTTC
NM_001110356.1      TTGCCGCAGCAGCTGCTGCAGCTGGTGCAGTTTCAAGAGGTGGATACAGATTTTGATTTC
NM_001001673.3      TTGCCGCAGCAGCTGCTGCAGCTGGTGCAGTTTCAAGAGGTGGATACAGATTTTGATTTC
NM_001110361.1      TTGCCGCAGCAGCTGCTGCAGCTGGTGCAGTTTCAAGAGGTGGATACAGATTTTGATTTC
NM_001110357.1      TTGCCGCAGCAGCTGCTGCAGCTGGTGCAGTTTCAAGAGGTGGATACAGATTTTGATTTC
NM_001110360.1      TTGCCGCAGCAGCTGCTGCAGCTGGTGCAGTTTCAAGAGGTGGATACAGATTTTGATTTC
NM_001110358.1      TTGCCGCAGCAGCTGCTGCAGCTGGTGCAGTTTCAAGAGGTGGATACAGATTTTGATTTC
                    ************************************************************

HsCD00443087        CCAGAAGAAGATAAAAAAGAAGAATTTGAAGAGTGTTTGGAAAAATTTTTTAGTACAGGG
NM_001110359.1      CCAGAAGAAGATAAAAAAGAAGAATTTGAAGAGTGTTTGGAAAAATTTTTTAGTACAGGG
NM_001289110.1      CCAGAAGAAGATAAAAAAGAAGAATTTGAAGAGTGTTTGGAAAAATTTTTTAGTACAGGG
NM_001110356.1      CCAGAAGAAGATAAAAAAGAAGAATTTGAAGAGTGTTTGGAAAAATTTTTTAGTACAGGG
NM_001001673.3      CCAGAAGAAGATAAAAAAGAAGAATTTGAAGAGTGTTTGGAAAAATTTTTTAGTACAGGG
NM_001110361.1      CCAGAAGAAGATAAAAAAGAAGAATTTGAAGAGTGTTTGGAAAAATTTTTTAGTACAGGG
NM_001110357.1      CCAGAAGAAGATAAAAAAGAAGAATTTGAAGAGTGTTTGGAAAAATTTTTTAGTACAGGG
NM_001110360.1      CCAGAAGAAGATAAAAAAGAAGAATTTGAAGAGTGTTTGGAAAAATTTTTTAGTACAGGG
NM_001110358.1      CCAGAAGAAGATAAAAAAGAAGAATTTGAAGAGTGTTTGGAAAAATTTTTTAGTACAGGG
                    ************************************************************

HsCD00443087        CCCGCCAGACCACCTACCAAAGAAAAAGTCAAAAGACGTGTCCTTATTGAACCTGGAATG
NM_001110359.1      CCCGCCAGACCACCTACCAAAGAAAAAGTCAAAAGACGTGTCCTTATTGAACCTGGAATG
NM_001289110.1      CCCGCCAGACCACCTACCAAAGAAAAAGTCAAAAGACGTGTCCTTATTGAACCTGGAATG
NM_001110356.1      CCCGCCAGACCACCTACCAAAGAAAAAGTCAAAAGACGTGTCCTTATTGAACCTGGAATG
NM_001001673.3      CCCGCCAGACCACCTACCAAAGAAAAAGTCAAAAGACGTGTCCTTATTGAACCTGGAATG
NM_001110361.1      CCCGCCAGACCACCTACCAAAGAAAAAGTCAAAAGACGTGTCCTTATTGAACCTGGAATG
NM_001110357.1      CCCGCCAGACCACCTACCAAAGAAAAAGTCAAAAGACGTGTCCTTATTGAACCTGGAATG
NM_001110360.1      CCCGCCAGACCACCTACCAAAGAAAAAGTCAAAAGACGTGTCCTTATTGAACCTGGAATG
NM_001110358.1      CCCGCCAGACCACCTACCAAAGAAAAAGTCAAAAGACGTGTCCTTATTGAACCTGGAATG
                    ************************************************************

HsCD00443087        CCACTAAATCATATAGAGTACTGTAACCATGAAATCATGGGAAAAAATGTTTACTACAAA
NM_001110359.1      CCACTAAATCATATAGAGTACTGTAACCATGAAATCATGGGAAAAAATGTTTACTACAAA
NM_001289110.1      CCACTAAATCATATAGAGTACTGTAACCATGAAATCATGGGAAAAAATGTTTACTACAAA
NM_001110356.1      CCACTAAATCATATAGAGTACTGTAACCATGAAATCATGGGAAAAAATGTTTACTACAAA
NM_001001673.3      CCACTAAATCATATAGAGTACTGTAACCATGAAATCATGGGAAAAAATGTTTACTACAAA
NM_001110361.1      CCACTAAATCATATAGAGTACTGTAACCATGAAATCATGGGAAAAAATGTTTACTACAAA
NM_001110357.1      CCACTAAATCATATAGAGTACTGTAACCATGAAATCATGGGAAAAAATGTTTACTACAAA
NM_001110360.1      CCACTAAATCATATAGAGTACTGTAACCATGAAATCATGGGAAAAAATGTTTACTACAAA
NM_001110358.1      CCACTAAATCATATAGAGTACTGTAACCATGAAATCATGGGAAAAAATGTTTACTACAAA
                    ************************************************************

HsCD00443087        CACCGTTGGGTGGCAGAACATTACTTCCTTCTTATGCAATATGACGAGCTCCAAAAAATC
NM_001110359.1      CACCGTTGGGTGGCAGAACATTACTTCCTTCTTATGCAATATGACGAGCTCCAAAAAATC
NM_001289110.1      CACCGTTGGGTGGCAGAACATTACTTCCTTCTTATGCAATATGACGAGCTCCAAAAAATC
NM_001110356.1      CACCGTTGGGTGGCAGAACATTACTTCCTTCTTATGCAATATGACGAGCTCCAAAAAATC
NM_001001673.3      CACCGTTGGGTGGCAGAACATTACTTCCTTCTTATGCAATATGACGAGCTCCAAAAAATC
NM_001110361.1      CACCGTTGGGTGGCAGAACATTACTTCCTTCTTATGCAATATGACGAGCTCCAAAAAATC
NM_001110357.1      CACCGTTGGGTGGCAGAACATTACTTCCTTCTTATGCAATATGACGAGCTCCAAAAAATC
NM_001110360.1      CACCGTTGGGTGGCAGAACATTACTTCCTTCTTATGCAATATGACGAGCTCCAAAAAATC
NM_001110358.1      CACCGTTGGGTGGCAGAACATTACTTCCTTCTTATGCAATATGACGAGCTCCAAAAAATC
                    ************************************************************

HsCD00443087        TGTTACAACAGATTTGTGCCATGTAAGAATGGAATTAGGAAATGTAACAGGAGCAAAGGT
NM_001110359.1      TGTTACAACAGATTTGTGCCATGTAAGAATGGAATTAGGAAATGTAACAGGAGCAAAGGT
NM_001289110.1      TGTTACAACAGATTTGTGCCATGTAAGAATGGAATTAGGAAATGTAACAGGAGCAAAGGT
NM_001110356.1      TGTTACAACAGATTTGTGCCATGTAAGAATGGAATTAGGAAATGTAACAGGAGCAAAGGT
NM_001001673.3      TGTTACAACAGATTTGTGCCATGTAAGAATGGAATTAGGAAATGTAACAGGAGCAAAGGT
NM_001110361.1      TGTTACAACAGATTTGTGCCATGTAAGAATGGAATTAGGAAATGTAACAGGAGCAAAGGT
NM_001110357.1      TGTTACAACAGATTTGTGCCATGTAAGAATGGAATTAGGAAATGTAACAGGAGCAAAGGT
NM_001110360.1      TGTTACAACAGATTTGTGCCATGTAAGAATGGAATTAGGAAATGTAACAGGAGCAAAGGT
NM_001110358.1      TGTTACAACAGATTTGTGCCATGTAAGAATGGAATTAGGAAATGTAACAGGAGCAAAGGT
                    ************************************************************

HsCD00443087        CTTGTAGAAGGAGTGTATTGTAATTTAACAGAAGCATCTGAAATACCAGCGTGTAAATAC
NM_001110359.1      CTTGTAGAAGGAGTGTATTGTAATTTAACAGAAGCATTTGAAATACCAGCGTGTAAATAC
NM_001289110.1      CTTGTAGAAGGAGTGTATTGTAATTTAACAGAAGCATTTGAAATACCAGCGTGTAAATAC
NM_001110356.1      CTTGTAGAAGGAGTGTATTGTAATTTAACAGAAGCATTTGAAATACCAGCGTGTAAATAC
NM_001001673.3      CTTGTAGAAGGAGTGTATTGTAATTTAACAGAAGCATTTGAAATACCAGCGTGTAAATAC
NM_001110361.1      CTTGTAGAAGGAGTGTATTGTAATTTAACAGAAGCATTTGAAATACCAGCGTGTAAATAC
NM_001110357.1      CTTGTAGAAGGAGTGTATTGTAATTTAACAGAAGCATTTGAAATACCAGCGTGTAAATAC
NM_001110360.1      CTTGTAGAAGGAGTGTATTGTAATTTAACAGAAGCATTTGAAATACCAGCGTGTAAATAC
NM_001110358.1      CTTGTAGAAGGAGTGTATTGTAATTTAACAGAAGCATTTGAAATACCAGCGTGTAAATAC
                    ************************************* **********************

HsCD00443087        GAATCACTTTATAGGAAGGGCTACGTCCTTATCACTTGTTCATGGCAAAATGAAATGCAA
NM_001110359.1      GAATCACTTTATAGGAAGGGCTACGTCCTTATCACTTGTTCATGGCAAAATGAAATGCAA
NM_001289110.1      GAATCACTTTATAGGAAGGGCTACGTCCTTATCACTTGTTCATGGCAAAATGAAATGCAA
NM_001110356.1      GAATCACTTTATAGGAAGGGCTACGTCCTTATCACTTGTTCATGGCAAAATGAAATGCAA
NM_001001673.3      GAATCACTTTATAGGAAGGGCTACGTCCTTATCACTTGTTCATGGCAAAATGAAATGCAA
NM_001110361.1      GAATCACTTTATAGGAAGGGCTACGTCCTTATCACTTGTTCATGGCAAAATGAAATGCAA
NM_001110357.1      GAATCACTTTATAGGAAGGGCTACGTCCTTATCACTTGTTCATGGCAAAATGAAATGCAA
NM_001110360.1      GAATCACTTTATAGGAAGGGCTACGTCCTTATCACTTGTTCATGGCAAAATGAAATGCAA
NM_001110358.1      GAATCACTTTATAGGAAGGGCTACGTCCTTATCACTTGTTCATGGCAAAATGAAATGCAA
                    ************************************************************

HsCD00443087        AAACGTATTCCTCATACTATAAATGATCTCGTGGAGCCACCTGAACACAGAAGTTTCCTC
NM_001110359.1      AAACGTATTCCTCATACTATAAATGATCTCGTGGAGCCACCTGAACACAGAAGTTTCCTC
NM_001289110.1      AAACGTATTCCTCATACTATAAATGATCTCGTGGAGCCACCTGAACACAGAAGTTTCCTC
NM_001110356.1      AAACGTATTCCTCATACTATAAATGATCTCGTGGAGCCACCTGAACACAGAAGTTTCCTC
NM_001001673.3      AAACGTATTCCTCATACTATAAATGATCTCGTGGAGCCACCTGAACACAGAAGTTTCCTC
NM_001110361.1      AAACGTATTCCTCATACTATAAATGATCTCGTGGAGCCACCTGAACACAGAAGTTTCCTC
NM_001110357.1      AAACGTATTCCTCATACTATAAATGATCTCGTGGAGCCACCTGAACACAGAAGTTTCCTC
NM_001110360.1      AAACGTATTCCTCATACTATAAATGATCTCGTGGAGCCACCTGAACACAGAAGTTTCCTC
NM_001110358.1      AAACGTATTCCTCATACTATAAATGATCTCGTGGAGCCACCTGAACACAGAAGTTTCCTC
                    ************************************************************

HsCD00443087        AGTGAGGATGGTGTCTTTGTCATACCGCCC---
NM_001110359.1      AGTGAGGATGGTGTCTTTGTCATATCGCCCTAG
NM_001289110.1      AGTGAGGATGGTGTCTTTGTCATATCGCCCTAG
NM_001110356.1      AGTGAGGATGGTGTCTTTGTCATATCGCCCTAG
NM_001001673.3      AGTGAGGATGGTGTCTTTGTCATATCGCCCTAG
NM_001110361.1      AGTGAGGATGGTGTCTTTGTCATATCGCCCTAG
NM_001110357.1      AGTGAGGATGGTGTCTTTGTCATATCGCCCTAG
NM_001110360.1      AGTGAGGATGGTGTCTTTGTCATATCGCCCTAG
NM_001110358.1      AGTGAGGATGGTGTCTTTGTCATATCGCCCTAG
                    ************************ *****   

Protein

SMART domain : RNAse_Pc : Pancreatic ribonuclease
HPRD : 17983

Clone

Original Clone ID : ccsbBroad304_10108

Organism


TAX_ID : 9606
Species Specific ID: 390443

Genome

Chromosome : 14
Map Location : 14q11.2
Ensembl : ENSG00000188655

Reagents

Labome : RNASE9-antibody