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KLHL28 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  KLHL28
Gene Name:  kelch-like family member 28
Sequence             
Original Clone ID: ccsbBroad304_03462
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00443516

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1714nts         Open reading frame : 1 to 1714
Reference Sequence Alignment

ATGGACCACACATCCCCGACCTACATGCTTGCTAACTTAACCCACTTGCATTCTGAACAA
CTTCTGCAGGGCTTGAATCTTCTTCGCCAACATCACGAACTCTGTGACATCATTCTTCGA
GTAGGTGATGTTAAAATTCATGCTCACAAAGTGGTACTTGCCAGCGTCAGCCCGTATTTC
AAAGCTATGTTCACTGGAAACCTTTCTGAAAAAGAGAACAGTGAGGTTGAGTTTCAATGC
ATTGATGAAACTGCTCTCCAGGCCATTGTGGAGTATGCCTATACAGGGACTGTTTTTATT
TCTCAGGACACAGTTGAATCTCTCCTGCCAGCAGCAAACCTACTCCAGATAAAACTTGTC
CTGAAAGAATGTTGTGCATTTCTTGAAAGCCAACTTGATCCTGGTAATTGTATTGGAATT
TCTCGTTTTGCAGAAACATATGGTTGCCGTGACCTTTATTTGGCAGCCACTAAATACATA
TGCCAGAATTTTGAAGCTGTTTGCCAGACTGAAGAGTTTTTTGAGCTTACACATGCTGAC
TTGGATGAAATTGTTTCCAATGACTGTTTGAATGTAGCTACCGAAGAGACTGTTTTTTAT
GCATTAGAGTCTTGGATCAAGTATGATGTACAAGAACGCCAGAAATACTTAGCACAGTTA
CTAAACAGTGTACGATTACCATTGTTGAGTGTTAAGTTTCTCACTAGACTATATGAAGCA
AATCATCTTATTCGTGATGATCGCACTTGTAAACATCTTTTGAATGAAGCCCTAAAGTAC
CACTTTATGCCTGAACATAGACTCTCTCATCAGACAGTCTTGATGACACGACCTCGCTGT
GCTCCCAAAGTACTTTGTGCAGTAGGAGGGAAATCTGGACTCTTTGCCTGTTTGGATAGT
GTGGAGATGTACTTTCCTCAGAATGACTCTTGGATTGGTTTGGCACCCCTAAACATTCCT
CGCTATGAATTTGGAATATGCGTTTTAGACCAAAAAGTATATGTTATAGGTGGTATTGCA
ACTAATGTGCGTCCTGGCGTCACTATCAGAAAACATGAAAATTCAGTGGAATGCTGGAAT
CCTGATACAAATACTTGGACTTCTCTAGAGAGAATGAATGAAAGCCGAAGTACTCTTGGA
GTAGTAGTACTTGCAGGAGAACTTTATGCCTTAGGTGGTTATGATGGACAATCTTATTTA
CAATCTGTAGAGAAGTACATTCCCAAAATAAGAAAATGGCAACCTGTGGCACCAATGACG
ACAACAAGAAGTTGTTTTGCTGCAGCGGTATTGGATGGAATGATATATGCCATTGGTGGG
TATGGTCCTGCCCACATGAACAGTGTGGAGCGTTATGATCCAAGTAAGGACTCCTGGGAG
ATGGTTGCATCCATGGCAGATAAAAGGATTCACTTTGGCGTGGGTGTCATGCTAGGCTTT
ATTTTTGTGGTGGGTGGACATAATGGAGTCTCACATTTGTCCAGCATTGAAAGATACGAT
CCTCATCAAAATCAGTGGACTGTGTGTAGACCAATGAAAGAACCTAGAACAGGAGTTGGT
GCTGCTGTAATCGATAACTACCTTTATGTCGTCGGTGGTCACTCAGGGTCTTCCTATCTG
AATACAGTGCAGAAATATGATCCTATCTCAGATACGTGGCTGGATTCAGCTGGCATGATA
TACTGTCGCTGCAACTTTGGGTTAACTGCACTT


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : KLHL28
Symbol Nomenclature : KLHL28
SYNONYM : BTBD5
Designation : BTB (POZ) domain containing 5|BTB/POZ domain-containing protein 5|kelch-like 28|kelch-like protein 28
Full Nomenclature : kelch-like family member 28
GENEID : 54813
HGNC : 19741
Vega : OTTHUMG00000140263
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00443516        ATGGACCACACATCCCCGACCTACATGCTTGCTAACTTAACCCACTTGCATTCTGAACAA
XM_005267770.1      ATGGACCACACATCCCCGACCTACATGCTTGCTAACTTAACCCACTTGCATTCTGAACAA
NM_017658.3         ATGGACCACACATCCCCGACCTACATGCTTGCTAACTTAACCCACTTGCATTCTGAACAA
                    ************************************************************

HsCD00443516        CTTCTGCAGGGCTTGAATCTTCTTCGCCAACATCACGAACTCTGTGACATCATTCTTCGA
XM_005267770.1      CTTCTGCAGGGCTTGAATCTTCTTCGCCAACATCACGAACTCTGTGACATCATTCTTCGA
NM_017658.3         CTTCTGCAGGGCTTGAATCTTCTTCGCCAACATCACGAACTCTGTGACATCATTCTTCGA
                    ************************************************************

HsCD00443516        GTAGGTGATGTTAAAATTCATGCTCACAAAGTGGTACTTGCCAGCGTCAGCCCGTATTTC
XM_005267770.1      GTAGGTGATGTTAAAATTCATGCTCACAAAGTGGTACTTGCCAGCGTCAGCCCGTATTTC
NM_017658.3         GTAGGTGATGTTAAAATTCATGCTCACAAAGTGGTACTTGCCAGCGTCAGCCCGTATTTC
                    ************************************************************

HsCD00443516        AAAGCTATGTTCACTGGAAACCTTTCTGAAAAAGAGAACAGTGAGGTTGAGTTTCAATGC
XM_005267770.1      AAAGCTATGTTCACTGGAAACCTTTCTGAAAAAGAGAACAGTGAGGTTGAGTTTCAATGC
NM_017658.3         AAAGCTATGTTCACTGGAAACCTTTCTGAAAAAGAGAACAGTGAGGTTGAGTTTCAATGC
                    ************************************************************

HsCD00443516        ATTGATGAAACTGCTCTCCAGGCCATTGTGGAGTATGCCTATACAGGGACTGTTTTTATT
XM_005267770.1      ATTGATGAAACTGCTCTCCAGGCCATTGTGGAGTATGCCTATACAGGGACTGTTTTTATT
NM_017658.3         ATTGATGAAACTGCTCTCCAGGCCATTGTGGAGTATGCCTATACAGGGACTGTTTTTATT
                    ************************************************************

HsCD00443516        TCTCAGGACACAGTTGAATCTCTCCTGCCAGCAGCAAACCTACTCCAGATAAAACTTGTC
XM_005267770.1      TCTCAGGACACAGTTGAATCTCTCCTGCCAGCAGCAAACCTACTCCAGATAAAACTTGTC
NM_017658.3         TCTCAGGACACAGTTGAATCTCTCCTGCCAGCAGCAAACCTACTCCAGATAAAACTTGTC
                    ************************************************************

HsCD00443516        CTGAAAGAATGTTGTGCATTTCTTGAAAGCCAACTTGATCCTGGTAATTGTATTGGAATT
XM_005267770.1      CTGAAAGAATGTTGTGCATTTCTTGAAAGCCAACTTGATCCTGGTAATTGTATTGGAATT
NM_017658.3         CTGAAAGAATGTTGTGCATTTCTTGAAAGCCAACTTGATCCTGGTAATTGTATTGGAATT
                    ************************************************************

HsCD00443516        TCTCGTTTTGCAGAAACATATGGTTGCCGTGACCTTTATTTGGCAGCCACTAAATACATA
XM_005267770.1      TCTCGTTTTGCAGAAACATATGGTTGCCGTGACCTTTATTTGGCAGCCACTAAATACATA
NM_017658.3         TCTCGTTTTGCAGAAACATATGGTTGCCGTGACCTTTATTTGGCAGCCACTAAATACATA
                    ************************************************************

HsCD00443516        TGCCAGAATTTTGAAGCTGTTTGCCAGACTGAAGAGTTTTTTGAGCTTACACATGCTGAC
XM_005267770.1      TGCCAGAATTTTGAAGCTGTTTGCCAGACTGAAGAGTTTTTTGAGCTTACACATGCTGAC
NM_017658.3         TGCCAGAATTTTGAAGCTGTTTGCCAGACTGAAGAGTTTTTTGAGCTTACACATGCTGAC
                    ************************************************************

HsCD00443516        TTGGATGAAATTGTTTCCAATGACTGTTTGAATGTAGCTACCGAAGAGACTGTTTTTTAT
XM_005267770.1      TTGGATGAAATTGTTTCCAATGACTGTTTGAATGTAGCTACCGAAGAGACTGTTTTTTAT
NM_017658.3         TTGGATGAAATTGTTTCCAATGACTGTTTGAATGTAGCTACCGAAGAGACTGTTTTTTAT
                    ************************************************************

HsCD00443516        GCATTAGAGTCTTGGATCAAGTATGATGTACAAGAACGCCAGAAATACTTAGCACAGTTA
XM_005267770.1      GCATTAGAGTCTTGGATCAAGTATGATGTACAAGAACGCCAGAAATACTTAGCACAGTTA
NM_017658.3         GCATTAGAGTCTTGGATCAAGTATGATGTACAAGAACGCCAGAAATACTTAGCACAGTTA
                    ************************************************************

HsCD00443516        CTAAACAGTGTACGATTACCATTGTTGAGTGTTAAGTTTCTCACTAGACTATATGAAGCA
XM_005267770.1      CTAAACAGTGTACGATTACCATTGTTGAGTGTTAAGTTTCTCACTAGACTATATGAAGCA
NM_017658.3         CTAAACAGTGTACGATTACCATTGTTGAGTGTTAAGTTTCTCACTAGACTATATGAAGCA
                    ************************************************************

HsCD00443516        AATCATCTTATTCGTGATGATCGCACTTGTAAACATCTTTTGAATGAAGCCCTAAAGTAC
XM_005267770.1      AATCATCTTATTCGTGATGATCGCACTTGTAAACATCTTTTGAATGAAGCCCTAAAGTAC
NM_017658.3         AATCATCTTATTCGTGATGATCGCACTTGTAAACATCTTTTGAATGAAGCCCTAAAGTAC
                    ************************************************************

HsCD00443516        CACTTTATGCCTGAACATAGACTCTCTCATCAGACAGTCTTGATGACACGACCTCGCTGT
XM_005267770.1      CACTTTATGCCTGAACATAGACTCTCTCATCAGACAGTCTTGATGACACGACCTCGCTGT
NM_017658.3         CACTTTATGCCTGAACATAGACTCTCTCATCAGACAGTCTTGATGACACGACCTCGCTGT
                    ************************************************************

HsCD00443516        GCTCCCAAAGTACTTTGTGCAGTAGGAGGGAAATCTGGACTCTTTGCCTGTTTGGATAGT
XM_005267770.1      GCTCCCAAAGTACTTTGTGCAGTAGGAGGGAAATCTGGACTCTTTGCCTGTTTGGATAGT
NM_017658.3         GCTCCCAAAGTACTTTGTGCAGTAGGAGGGAAATCTGGACTCTTTGCCTGTTTGGATAGT
                    ************************************************************

HsCD00443516        GTGGAGATGTACTTTCCTCAGAATGACTCTTGGATTGGTTTGGCACCCCTAAACATTCCT
XM_005267770.1      GTGGAGATGTACTTTCCTCAGAATGACTCTTGGATTGGTTTGGCACCCCTAAACATTCCT
NM_017658.3         GTGGAGATGTACTTTCCTCAGAATGACTCTTGGATTGGTTTGGCACCCCTAAACATTCCT
                    ************************************************************

HsCD00443516        CGCTATGAATTTGGAATATGCGTTTTAGACCAAAAAGTATATGTTATAGGTGGTATTGCA
XM_005267770.1      CGCTATGAATTTGGAATATGCGTTTTAGACCAAAAAGTATATGTTATAGGTGGTATTGCA
NM_017658.3         CGCTATGAATTTGGAATATGCGTTTTAGACCAAAAAGTATATGTTATAGGTGGTATTGCA
                    ************************************************************

HsCD00443516        ACTAATGTGCGTCCTGGCGTCACTATCAGAAAACATGAAAATTCAGTGGAATGCTGGAAT
XM_005267770.1      ACTAATGTGCGTCCTGGCGTCACTATCAGAAAACATGAAAATTCAGTGGAATGCTGGAAT
NM_017658.3         ACTAATGTGCGTCCTGGCGTCACTATCAGAAAACATGAAAATTCAGTGGAATGCTGGAAT
                    ************************************************************

HsCD00443516        CCTGATACAAATACTTGGACTTCTCTAGAGAGAATGAATGAAAGCCGAAGTACTCTTGGA
XM_005267770.1      CCTGATACAAATACTTGGACTTCTCTAGAGAGAATGAATGAAAGCCGAAGTACTCTTGGA
NM_017658.3         CCTGATACAAATACTTGGACTTCTCTAGAGAGAATGAATGAAAGCCGAAGTACTCTTGGA
                    ************************************************************

HsCD00443516        GTAGTAGTACTTGCAGGAGAACTTTATGCCTTAGGTGGTTATGATGGACAATCTTATTTA
XM_005267770.1      GTAGTAGTACTTGCAGGAGAACTTTATGCCTTAGGTGGTTATGATGGACAATCTTATTTA
NM_017658.3         GTAGTAGTACTTGCAGGAGAACTTTATGCCTTAGGTGGTTATGATGGACAATCTTATTTA
                    ************************************************************

HsCD00443516        CAATCTGTAGAGAAGTACATTCCCAAAATAAGAAAATGGCAACCTGTGGCACCAATGACG
XM_005267770.1      CAATCTGTAGAGAAGTACATTCCCAAAATAAGAAAATGGCAACCTGTGGCACCAATGACG
NM_017658.3         CAATCTGTAGAGAAGTACATTCCCAAAATAAGAAAATGGCAACCTGTGGCACCAATGACG
                    ************************************************************

HsCD00443516        ACAACAAGAAGTTGTTTTGCTGCAGCGGTATTGGATGGAATGATATATGCCATTGGTGGG
XM_005267770.1      ACAACAAGAAGTTGTTTTGCTGCAGCGGTATTGGATGGAATGATATATGCCATTGGTGGG
NM_017658.3         ACAACAAGAAGTTGTTTTGCTGCAGCGGTATTGGATGGAATGATATATGCCATTGGTGGG
                    ************************************************************

HsCD00443516        TATGGTCCTGCCCACATGAACAGTGTGGAGCGTTATGATCCAAGTAAGGACTCCTGGGAG
XM_005267770.1      TATGGTCCTGCCCACATGAACAGTGTGGAGCGTTATGATCCAAGTAAGGACTCCTGGGAG
NM_017658.3         TATGGTCCTGCCCACATGAACAGTGTGGAGCGTTATGATCCAAGTAAGGACTCCTGGGAG
                    ************************************************************

HsCD00443516        ATGGTTGCATCCATGGCAGATAAAAGGATTCACTTTGGCGTGGGTGTCATGCTAGGCTTT
XM_005267770.1      ATGGTTGCATCCATGGCAGATAAAAGGATTCACTTTGGCGTGGGTGTCATGCTAGGCTTT
NM_017658.3         ATGGTTGCATCCATGGCAGATAAAAGGATTCACTTTGGCGTGGGTGTCATGCTAGGCTTT
                    ************************************************************

HsCD00443516        ATTTTTGTGGTGGGTGGACATAATGGAGTCTCACATTTGTCCAGCATTGAAAGATACGAT
XM_005267770.1      ATTTTTGTGGTGGGTGGACATAATGGAGTCTCACATTTGTCCAGCATTGAAAGATACGAT
NM_017658.3         ATTTTTGTGGTGGGTGGACATAATGGAGTCTCACATTTGTCCAGCATTGAAAGATACGAT
                    ************************************************************

HsCD00443516        CCTCATCAAAATCAGTGGACTGTGTGTAGACCAATGAAAGAACCTAGAACAGGAGTTGGT
XM_005267770.1      CCTCATCAAAATCAGTGGACTGTGTGTAGACCAATGAAAGAACCTAGAACAGGAGTTGGT
NM_017658.3         CCTCATCAAAATCAGTGGACTGTGTGTAGACCAATGAAAGAACCTAGAACAGGAGTTGGT
                    ************************************************************

HsCD00443516        GCTGCTGTAATCGATAACTACCTTTATGTCGTCGGTGGTCACTCAGGGTCTTCCTATCTG
XM_005267770.1      GCTGCTGTAATCGATAACTACCTTTATGTCGTCGGTGGTCACTCAGGGTCTTCCTATCTG
NM_017658.3         GCTGCTGTAATCGATAACTACCTTTATGTCGTCGGTGGTCACTCAGGGTCTTCCTATCTG
                    ************************************************************

HsCD00443516        AATACAGTGCAGAAATATGATCCTATCTCAGATACGTGGCTGGATTCAGCTGGCATGATA
XM_005267770.1      AATACAGTGCAGAAATATGATCCTATCTCAGATACGTGGCTGGATTCAGCTGGCATGATA
NM_017658.3         AATACAGTGCAGAAATATGATCCTATCTCAGATACGTGGCTGGATTCAGCTGGCATGATA
                    ************************************************************

HsCD00443516        TACTGTCGCTGCAACTTTGGGTTAACTGCACTT---
XM_005267770.1      TACTGTCGCTGCAACTTTGGGTTAACTGCACTTTGA
NM_017658.3         TACTGTCGCTGCAACTTTGGGTTAACTGCACTTTGA
                    *********************************   

Protein

SMART domain : Kelch :
SMART domain : BACK : BTB And C-terminal Kelch
SMART domain : BTB : Broad-Complex, Tramtrack and Bric a brac
HPRD : 12539

Clone

Original Clone ID : ccsbBroad304_03462

Organism


TAX_ID : 9606
Species Specific ID: 54813

Genome

Chromosome : 14
Map Location : 14q21.2
Ensembl : ENSG00000179454

Reagents

Labome : KLHL28-antibody