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KBTBD5 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  KLHL40
Gene Name:  kelch-like family member 40
Sequence             
Original Clone ID: ccsbBroad304_09532
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00443547

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1864nts         Open reading frame : 1 to 1864
Reference Sequence Alignment

ATGGCGCTGGGCTTGGAGCAGGCGGAGGAGCAGCGGTTGTACCAGCAGACGCTCCTGCAA
GACGGGCTCAAAGACATGCTGGACCATGGCAAGTTCCTCGACTGTGTGGTGCGGGCGGGC
GAGCGCGAGTTCCCGTGCCATCGCCTGGTGCTGGCCGCCTGCAGCCCCTACTTCCGGGCG
CGCTTTCTAGCCGAGCCGGAGCGCGCGGGCGAGCTGCACCTGGAGGAGGTGTCCCCGGAC
GTGGTGGCCCAGGTGCTGCACTACCTGTACACATCAGAGATCGCGCTGGATGAGGCGAGC
GTGCAGGATTTGTTCGCCGCGGCACACCGCTTCCAGATCCCTTCCATCTTCACCATCTGC
GTGTCCTTCCTGCAGAAGCGCCTGTGCCTCTCCAACTGCTTGGCCGTCTTCCGTCTCGGC
CTCCTGCTCGACTGCGCGCGTCTCGCCGTGGCTGCCCGCGACTTCATCTGCGCTCACTTC
ACGCTGGTGGCGCGCGACGCTGACTTCCTCGGACTCTCGGCCGACGAGCTCATCGCCATC
ATCTCCAGCGACGGCCTTAACGTGGAGAAGGAGGAGGCAGTGTTCGAGGCGGTGATGCGG
TGGGCGGGTAGCGGCGACGCCGAGGCGCAGGCTGAGCGCCAGCGCGCGCTGCCCACCGTC
TTCGAGAGCGTGCGCTGCCGCTTGCTGCCGCGCGCCTTTCTGGAAAGCCGCGTGGAGCGC
CACCCTCTCGTGCGTGCCCAGCCCGAGTTGCTGCGCAAGGTGCAGATGGTGAAGGATGCA
CACGAGGGCCGCATCACCACGCTGCGGAAGAAAAAGAAGGGGAAGGATGGAGCCGGGGCC
AAGGAGGCTGATAAGGGCACAAGCAAAGCCAAAGCAGAGGAGGATGAGGAGGCCGAACGT
ATCCTTCCTGGGATCCTCAATGACACCCTGCGCTTCGGCATGTTCCTGCAGGATCTCATC
TTCATGATCAGTGAGGAGGGCGCTGTGGCCTACGATCCAGCAGCCAACGAGTGCTACTGT
GCTTCCCTCTCCAGCCAGGTCCCCAAGAACCACGTCAGCCTGGTTACCAAGGAGAACCAG
GTCTTCGTGGCTGGAGGCCTCTTCTACAACGAAGACAACAAAGAGGACCCCATGAGCGCA
TACTTCCTGCAGTTTGACCATCTGGACTCAGAGTGGCTGGGGATGCCACCGCTGCCCTCG
CCCCGCTGCCTCTTTGGCCTGGGAGAAGCTCTCAACTCCATCTACGTGGTCGGTGGCAGA
GAGATCAAGGACGGCGAGCGCTGCCTGGACTCGGTCATGTGCTACGACAGGCTGTCATTC
AAATGGGGTGAATCGGACCCGCTGCCTTACGTGGTGTATGGCCACACAGTGCTCTCCCAC
ATGGACCTTGTCTACGTAATTGGCGGCAAAGGCAGTGACAGGAAGTGCCTGAACAAGATG
TGCGTCTATGACCCCAAGAAGTTTGAGTGGAAGGAGCTGGCACCCATGCAGACCGCCCGC
TCACTCTTTGGGGCCACTGTCCATGATGGCCGCATTATCGTGGCAGCTGGGGTCACCGAC
ACAGGGCTGACCAGTTCTGCCGAAGTGTACAGCATCACAGACAACAAGTGGGCACCCTTC
GAGGCCTTCCCACAGGAGCGTAGCTCACTCAGCCTGGTCAGCCTGGTGGGTACCCTCTAT
GCCATTGGTGGCTTTGCCACACTGGAGACGGAGTCTGGAGAGCTGGTTCCCACAGAGCTC
AATGACATCTGGAGGTATAACGAGGAGGAGAAGAAATGGGAGGGTGTCCTGCGGGAGATC
GCCTATGCAGCAGGTGCCACCTTCCTACCAGTGCGGCTCAATGTGCTGCGCCTGACTAAG
ATG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : KLHL40
Symbol Nomenclature : KLHL40
SYNONYM : KBTBD5; NEM8; SRYP; SYRP
Designation : kelch repeat and BTB (POZ) domain containing 5|kelch repeat and BTB domain-containing protein 5|kelch-like 40|kelch-like protein 40|nemaline myopathy type 8|sarcosynapsin
Full Nomenclature : kelch-like family member 40
GENEID : 131377
HGNC : 30372
MIM : 615340
Vega : OTTHUMG00000133045
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00443547      ATGGCGCTGGGCTTGGAGCAGGCGGAGGAGCAGCGGTTGTACCAGCAGACGCTCCTGCAA
NM_152393.3       ATGGCGCTGGGCTTGGAGCAGGCGGAGGAGCAGCGGTTGTACCAGCAGACGCTCCTGCAA
                  ************************************************************

HsCD00443547      GACGGGCTCAAAGACATGCTGGACCATGGCAAGTTCCTCGACTGTGTGGTGCGGGCGGGC
NM_152393.3       GACGGGCTCAAAGACATGCTGGACCATGGCAAGTTCCTCGACTGTGTGGTGCGGGCGGGC
                  ************************************************************

HsCD00443547      GAGCGCGAGTTCCCGTGCCATCGCCTGGTGCTGGCCGCCTGCAGCCCCTACTTCCGGGCG
NM_152393.3       GAGCGCGAGTTCCCGTGCCATCGCCTGGTGCTGGCCGCCTGCAGCCCCTACTTCCGGGCG
                  ************************************************************

HsCD00443547      CGCTTTCTAGCCGAGCCGGAGCGCGCGGGCGAGCTGCACCTGGAGGAGGTGTCCCCGGAC
NM_152393.3       CGCTTTCTAGCCGAGCCGGAGCGCGCGGGCGAGCTGCACCTGGAGGAGGTGTCCCCGGAC
                  ************************************************************

HsCD00443547      GTGGTGGCCCAGGTGCTGCACTACCTGTACACATCAGAGATCGCGCTGGATGAGGCGAGC
NM_152393.3       GTGGTGGCCCAGGTGCTGCACTACCTGTACACATCAGAGATCGCGCTGGATGAGGCGAGC
                  ************************************************************

HsCD00443547      GTGCAGGATTTGTTCGCCGCGGCACACCGCTTCCAGATCCCTTCCATCTTCACCATCTGC
NM_152393.3       GTGCAGGATTTGTTCGCCGCGGCACACCGCTTCCAGATCCCTTCCATCTTCACCATCTGC
                  ************************************************************

HsCD00443547      GTGTCCTTCCTGCAGAAGCGCCTGTGCCTCTCCAACTGCTTGGCCGTCTTCCGTCTCGGC
NM_152393.3       GTGTCCTTCCTGCAGAAGCGCCTGTGCCTCTCCAACTGCTTGGCCGTCTTCCGTCTCGGC
                  ************************************************************

HsCD00443547      CTCCTGCTCGACTGCGCGCGTCTCGCCGTGGCTGCCCGCGACTTCATCTGCGCTCACTTC
NM_152393.3       CTCCTGCTCGACTGCGCGCGTCTCGCCGTGGCTGCCCGCGACTTCATCTGCGCTCACTTC
                  ************************************************************

HsCD00443547      ACGCTGGTGGCGCGCGACGCTGACTTCCTCGGACTCTCGGCCGACGAGCTCATCGCCATC
NM_152393.3       ACGCTGGTGGCGCGCGACGCTGACTTCCTCGGACTCTCGGCCGACGAGCTCATCGCCATC
                  ************************************************************

HsCD00443547      ATCTCCAGCGACGGCCTTAACGTGGAGAAGGAGGAGGCAGTGTTCGAGGCGGTGATGCGG
NM_152393.3       ATCTCCAGCGACGGCCTTAACGTGGAGAAGGAGGAGGCAGTGTTCGAGGCGGTGATGCGG
                  ************************************************************

HsCD00443547      TGGGCGGGTAGCGGCGACGCCGAGGCGCAGGCTGAGCGCCAGCGCGCGCTGCCCACCGTC
NM_152393.3       TGGGCGGGTAGCGGCGACGCCGAGGCGCAGGCTGAGCGCCAGCGCGCGCTGCCCACCGTC
                  ************************************************************

HsCD00443547      TTCGAGAGCGTGCGCTGCCGCTTGCTGCCGCGCGCCTTTCTGGAAAGCCGCGTGGAGCGC
NM_152393.3       TTCGAGAGCGTGCGCTGCCGCTTGCTGCCGCGCGCCTTTCTGGAAAGCCGCGTGGAGCGC
                  ************************************************************

HsCD00443547      CACCCTCTCGTGCGTGCCCAGCCCGAGTTGCTGCGCAAGGTGCAGATGGTGAAGGATGCA
NM_152393.3       CACCCTCTCGTGCGTGCCCAGCCCGAGTTGCTGCGCAAGGTGCAGATGGTGAAGGATGCA
                  ************************************************************

HsCD00443547      CACGAGGGCCGCATCACCACGCTGCGGAAGAAAAAGAAGGGGAAGGATGGAGCCGGGGCC
NM_152393.3       CACGAGGGCCGCATCACCACGCTGCGGAAGAAAAAGAAGGGGAAGGATGGAGCCGGGGCC
                  ************************************************************

HsCD00443547      AAGGAGGCTGATAAGGGCACAAGCAAAGCCAAAGCAGAGGAGGATGAGGAGGCCGAACGT
NM_152393.3       AAGGAGGCTGATAAGGGCACAAGCAAAGCCAAAGCAGAGGAGGATGAGGAGGCCGAACGT
                  ************************************************************

HsCD00443547      ATCCTTCCTGGGATCCTCAATGACACCCTGCGCTTCGGCATGTTCCTGCAGGATCTCATC
NM_152393.3       ATCCTTCCTGGGATCCTCAATGACACCCTGCGCTTCGGCATGTTCCTGCAGGATCTCATC
                  ************************************************************

HsCD00443547      TTCATGATCAGTGAGGAGGGCGCTGTGGCCTACGATCCAGCAGCCAACGAGTGCTACTGT
NM_152393.3       TTCATGATCAGTGAGGAGGGCGCTGTGGCCTACGATCCAGCAGCCAACGAGTGCTACTGT
                  ************************************************************

HsCD00443547      GCTTCCCTCTCCAGCCAGGTCCCCAAGAACCACGTCAGCCTGGTTACCAAGGAGAACCAG
NM_152393.3       GCTTCCCTCTCCAACCAGGTCCCCAAGAACCACGTCAGCCTGGTTACCAAGGAGAACCAG
                  *************.**********************************************

HsCD00443547      GTCTTCGTGGCTGGAGGCCTCTTCTACAACGAAGACAACAAAGAGGACCCCATGAGCGCA
NM_152393.3       GTCTTCGTGGCTGGAGGCCTCTTCTACAACGAAGACAACAAAGAGGACCCCATGAGCGCA
                  ************************************************************

HsCD00443547      TACTTCCTGCAGTTTGACCATCTGGACTCAGAGTGGCTGGGGATGCCACCGCTGCCCTCG
NM_152393.3       TACTTCCTGCAGTTTGACCATCTGGACTCAGAGTGGCTGGGGATGCCACCGCTGCCCTCG
                  ************************************************************

HsCD00443547      CCCCGCTGCCTCTTTGGCCTGGGAGAAGCTCTCAACTCCATCTACGTGGTCGGTGGCAGA
NM_152393.3       CCCCGCTGCCTCTTTGGCCTGGGAGAAGCTCTCAACTCCATCTACGTGGTCGGTGGCAGA
                  ************************************************************

HsCD00443547      GAGATCAAGGACGGCGAGCGCTGCCTGGACTCGGTCATGTGCTACGACAGGCTGTCATTC
NM_152393.3       GAGATCAAGGACGGCGAGCGCTGCCTGGACTCGGTCATGTGCTACGACAGGCTGTCATTC
                  ************************************************************

HsCD00443547      AAATGGGGTGAATCGGACCCGCTGCCTTACGTGGTGTATGGCCACACAGTGCTCTCCCAC
NM_152393.3       AAATGGGGTGAATCGGACCCGCTGCCTTACGTGGTGTATGGCCACACAGTGCTCTCCCAC
                  ************************************************************

HsCD00443547      ATGGACCTTGTCTACGTAATTGGCGGCAAAGGCAGTGACAGGAAGTGCCTGAACAAGATG
NM_152393.3       ATGGACCTTGTCTACGTAATTGGCGGCAAAGGCAGTGACAGGAAGTGCCTGAACAAGATG
                  ************************************************************

HsCD00443547      TGCGTCTATGACCCCAAGAAGTTTGAGTGGAAGGAGCTGGCACCCATGCAGACCGCCCGC
NM_152393.3       TGCGTCTATGACCCCAAGAAGTTTGAGTGGAAGGAGCTGGCACCCATGCAGACCGCCCGC
                  ************************************************************

HsCD00443547      TCACTCTTTGGGGCCACTGTCCATGATGGCCGCATTATCGTGGCAGCTGGGGTCACCGAC
NM_152393.3       TCACTCTTTGGGGCCACTGTCCATGATGGCCGCATTATCGTGGCAGCTGGGGTCACCGAC
                  ************************************************************

HsCD00443547      ACAGGGCTGACCAGTTCTGCCGAAGTGTACAGCATCACAGACAACAAGTGGGCACCCTTC
NM_152393.3       ACAGGGCTGACCAGTTCTGCCGAAGTGTACAGCATCACAGACAACAAGTGGGCACCCTTC
                  ************************************************************

HsCD00443547      GAGGCCTTCCCACAGGAGCGTAGCTCACTCAGCCTGGTCAGCCTGGTGGGTACCCTCTAT
NM_152393.3       GAGGCCTTCCCACAGGAGCGTAGCTCACTCAGCCTGGTCAGCCTGGTGGGTACCCTCTAT
                  ************************************************************

HsCD00443547      GCCATTGGTGGCTTTGCCACACTGGAGACGGAGTCTGGAGAGCTGGTTCCCACAGAGCTC
NM_152393.3       GCCATTGGTGGCTTTGCCACACTGGAGACGGAGTCTGGAGAGCTGGTTCCCACAGAGCTC
                  ************************************************************

HsCD00443547      AATGACATCTGGAGGTATAACGAGGAGGAGAAGAAATGGGAGGGTGTCCTGCGGGAGATC
NM_152393.3       AATGACATCTGGAGGTATAACGAGGAGGAGAAGAAATGGGAGGGTGTCCTGCGGGAGATC
                  ************************************************************

HsCD00443547      GCCTATGCAGCAGGTGCCACCTTCCTACCAGTGCGGCTCAATGTGCTGCGCCTGACTAAG
NM_152393.3       GCCTATGCAGCAGGTGCCACCTTCCTACCAGTGCGGCTCAATGTGCTGTGCCTGACTAAG
                  ************************************************ ***********

HsCD00443547      ATG---
NM_152393.3       ATGTGA
                  ***   

Protein

SMART domain : BACK : BTB And C-terminal Kelch
SMART domain : BTB : Broad-Complex, Tramtrack and Bric a brac
SMART domain : Kelch :
HPRD : 11058

Clone

Original Clone ID : ccsbBroad304_09532

Organism


TAX_ID : 9606
Species Specific ID: 131377

Genome

Chromosome : 3
Map Location : 3p22.1
Ensembl : ENSG00000157119

Reagents

Labome : KLHL40-antibody