DNASU Plasmid Repository • 480.965.5697 | Email

EDDM3B (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  EDDM3B
Gene Name:  epididymal protein 3B
Sequence             
Original Clone ID: ccsbBroad304_08842
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00443690

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 442nts         Open reading frame : 1 to 442
Reference Sequence Alignment

ATGGCATCGTCTGTAAAGATCTGGGGCACACTCTTGGCCCTACTTTGCATCCTATGCACA
CTGCTTGTACAGAGCAAAGAAGTTTCTTGGAGAGAATTCATGAAACAGCACTACTTAAGT
CCAAGTCGAGAATTCAGAGAGTACAAATGTGATGTCCTCATGAGAGAAAATGAAGCTCTG
AAAGACAAGAGCTCTCACATGTTTATCTATATCTCATGGTACAAAATCGAGCATATATGC
ACTAGTGACAACTGGATGGATCGCTTCCGAAATGCATATGTATGGGTCCAGAATCCTCTC
AAAGTACTCAAGTGTCACCAGGAGAATTCCAAAAATAGCTACACAGAGAGCAGGAGCTTC
AACTACATTGAATTCCATTGTAGCATGGACGGGTATGTTGATAGCATAGAAGACCTAAAG
ATGGTAGAACCTATCGGCAAC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : EDDM3B
Symbol Nomenclature : EDDM3B
SYNONYM : EP3B; FAM12B; HE3-BETA; HE3B; RAM2
Designation : epididymal secretory protein E3 beta|epididymal secretory protein E3-beta|family with sequence similarity 12, member B (epididymal)|human epididymis-specific 3 beta|human epididymis-specific protein 3-beta|ribonuclease A M2
Full Nomenclature : epididymal protein 3B
GENEID : 64184
Locus Tag : UNQ6412/PRO21187
HGNC : 19223
MIM : 611582
Vega : OTTHUMG00000029583
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00443690      ATGGCATCGTCTGTAAAGATCTGGGGCACACTCTTGGCCCTACTTTGCATCCTATGCACA
NM_022360.4       ATGGCATCGTCTCTAAAGATCTGGGGCACACTCTTGGCCCTACTTTGCATCCTATGCACA
                  ************ ***********************************************

HsCD00443690      CTGCTTGTACAGAGCAAAGAAGTTTCTTGGAGAGAATTCATGAAACAGCACTACTTAAGT
NM_022360.4       CTGCTTGTACAGAGCAAAGAAGTTTCTTGGAGAGAATTCATGAAACAGCACTACTTAAGT
                  ************************************************************

HsCD00443690      CCAAGTCGAGAATTCAGAGAGTACAAATGTGATGTCCTCATGAGAGAAAATGAAGCTCTG
NM_022360.4       CCAAGTCGAGAATTCAGAGAGTACAAATGTGATGTCCTCATGAGAGAAAATGAAGCTCTG
                  ************************************************************

HsCD00443690      AAAGACAAGAGCTCTCACATGTTTATCTATATCTCATGGTACAAAATCGAGCATATATGC
NM_022360.4       AAAGACAAGAGCTCTCACATGTTTATCTATATCTCATGGTACAAAATCGAGCATATATGC
                  ************************************************************

HsCD00443690      ACTAGTGACAACTGGATGGATCGCTTCCGAAATGCATATGTATGGGTCCAGAATCCTCTC
NM_022360.4       ACTAGTGACAACTGGATGGATCGCTTCCGAAATGCATATGTATGGGTCCAGAATCCTCTC
                  ************************************************************

HsCD00443690      AAAGTACTCAAGTGTCACCAGGAGAATTCCAAAAATAGCTACACAGAGAGCAGGAGCTTC
NM_022360.4       AAAGTACTCAAGTGTCACCAGGAGAATTCCAAAAATAGCTACACAGAGAGCAGGAGCTTC
                  ************************************************************

HsCD00443690      AACTACATTGAATTCCATTGTAGCATGGACGGGTATGTTGATAGCATAGAAGACCTAAAG
NM_022360.4       AACTACATTGAATTCCATTGTAGCATGGACGGGTATGTTGATAGCATAGAAGACCTAAAG
                  ************************************************************

HsCD00443690      ATGGTAGAACCTATCGGCAAC---
NM_022360.4       ATGGTAGAACCTATCGGCAACTAG
                  *********************   

Protein

SMART domain : RNAse_Pc : Pancreatic ribonuclease
HPRD : 13290

Clone

Original Clone ID : ccsbBroad304_08842

Organism


TAX_ID : 9606
Species Specific ID: 64184

Genome

Chromosome : 14
Map Location : 14q11.2
Ensembl : ENSG00000181552

Reagents

Labome : EDDM3B-antibody