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CHRNA1 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  CHRNA1
Gene Name:  cholinergic receptor, nicotinic, alpha 1 (muscle)
Sequence             
Original Clone ID: ccsbBroad304_10733
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00444007

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 808nts         Open reading frame : 1 to 808
Reference Sequence Alignment

ATGGAGCCCTGGCCTCTCCTCCTGCTCTTTAGCCTTTGCTCAGCTGGCCTCGTCCTGGGC
TCCGAACATGAGACCCGTCTGGTGGCAAAGCTATTTAAAGACTACAGCAGCGTGGTGCGG
CCAGTGGAAGACCACCGCCAGGTCGTGGAGGTCACCGTGGGCCTGCAGCTGATACAGCTC
ATCAATGTGGATGAAGTAAATCAGATCGTGACAACCAATGTGCGTCTGAAACAGCAATGG
GTGGATTACAACCTAAAATGGAATCCAGATGACTATGGCGGTGTGAAAAAAATTCACATT
CCTTCAGAAAAGATCTGGCGCCCAGACCTTGTTCTCTATAACAATGCAGATGGTGACTTT
GCTATTGTCAAGTTCACCAAAGTGCTCCTGCAGTACACTGGCCACATCACGTGGACACCT
CCAGCCATCTTTAAAAGCTACTGTGAGATCATCGTCACCCACTTTCCCTTTGATGAACAG
AACTGCAGCATGAAGCTGGGCACCTGGACCTACGACGGCTCTGTCGTGGCCATCAACCCG
GAAAGCGACCAGCCAGACCTGAGCAACTTCATGGAGAGCGGGGAGTGGGTGATCAAGGAG
TCCCGGGGCTGGAAGCACTCCGTGACCTATTCCTGCTGCCCCGACACCCCCTACCTGGAC
ATCACCTACCACTTCGTCATGCAGCGCCTGCCCCTCTACTTCATCGTCAACGTCATCATC
CCCTGCCTGCTCTTCTCCTTCTTAACTGGCCTGGTATTCTACCTGCCCACAGACTCAGGT
GGGTGTGGTTGCCATGACTGCTGCTGC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CHRNA1
Symbol Nomenclature : CHRNA1
SYNONYM : ACHRA; ACHRD; CHRNA; CMS2A; FCCMS; SCCMS
Designation : acetylcholine receptor subunit alpha|acetylcholine receptor, nicotinic, alpha 1 (muscle)|cholinergic receptor, nicotinic, alpha polypeptide 1 (muscle)|muscle nicotinic acetylcholine receptor|nicotinic acetylcholine receptor alpha subunit|nicotinic cholinergic receptor alpha 1
Full Nomenclature : cholinergic receptor, nicotinic, alpha 1 (muscle)
GENEID : 1134
HGNC : 1955
MIM : 100690
Vega : OTTHUMG00000132357
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00444007        ATGGAGCCCTGGCCTCTCCTCCTGCTCTTTAGCCTTTGCTCAGCTGGCCTCGTCCTGGGC
NM_000079.3         ATGGAGCCCTGGCCTCTCCTCCTGCTCTTTAGCCTTTGCTCAGCTGGCCTCGTCCTGGGC
NM_001039523.2      ATGGAGCCCTGGCCTCTCCTCCTGCTCTTTAGCCTTTGCTCAGCTGGCCTCGTCCTGGGC
                    ************************************************************

HsCD00444007        TCCGAACATGAGACCCGTCTGGTGGCAAAGCTATTTAAAGACTACAGCAGCGTGGTGCGG
NM_000079.3         TCCGAACATGAGACCCGTCTGGTGGCAAAGCTATTTAAAGACTACAGCAGCGTGGTGCGG
NM_001039523.2      TCCGAACATGAGACCCGTCTGGTGGCAAAGCTATTTAAAGACTACAGCAGCGTGGTGCGG
                    ************************************************************

HsCD00444007        CCAGTGGAAGACCACCGCCAGGTCGTGGAGGTCACCGTGGGCCTGCAGCTGATACAGCTC
NM_000079.3         CCAGTGGAAGACCACCGCCAGGTCGTGGAGGTCACCGTGGGCCTGCAGCTGATACAGCTC
NM_001039523.2      CCAGTGGAAGACCACCGCCAGGTCGTGGAGGTCACCGTGGGCCTGCAGCTGATACAGCTC
                    ************************************************************

HsCD00444007        ATCAATGTGGATGAAGTAAATCAGATCGTGACAACCAATGTGCGTCTGAAACAG------
NM_000079.3         ATCAATGTGGATGAAGTAAATCAGATCGTGACAACCAATGTGCGTCTGAAACAG------
NM_001039523.2      ATCAATGTGGATGAAGTAAATCAGATCGTGACAACCAATGTGCGTCTGAAACAGGGTGAC
                    ******************************************************      

HsCD00444007        ------------------------------------------------------------
NM_000079.3         ------------------------------------------------------------
NM_001039523.2      ATGGTAGATCTGCCACGCCCCAGCTGCGTGACTTTGGGAGTTCCTTTGTTTTCTCATCTG
                                                                                

HsCD00444007        ---------CAATGGGTGGATTACAACCTAAAATGGAATCCAGATGACTATGGCGGTGTG
NM_000079.3         ---------CAATGGGTGGATTACAACCTAAAATGGAATCCAGATGACTATGGCGGTGTG
NM_001039523.2      CAGAATGAGCAATGGGTGGATTACAACCTAAAATGGAATCCAGATGACTATGGCGGTGTG
                             ***************************************************

HsCD00444007        AAAAAAATTCACATTCCTTCAGAAAAGATCTGGCGCCCAGACCTTGTTCTCTATAACAAT
NM_000079.3         AAAAAAATTCACATTCCTTCAGAAAAGATCTGGCGCCCAGACCTTGTTCTCTATAACAAT
NM_001039523.2      AAAAAAATTCACATTCCTTCAGAAAAGATCTGGCGCCCAGACCTTGTTCTCTATAACAAT
                    ************************************************************

HsCD00444007        GCAGATGGTGACTTTGCTATTGTCAAGTTCACCAAAGTGCTCCTGCAGTACACTGGCCAC
NM_000079.3         GCAGATGGTGACTTTGCTATTGTCAAGTTCACCAAAGTGCTCCTGCAGTACACTGGCCAC
NM_001039523.2      GCAGATGGTGACTTTGCTATTGTCAAGTTCACCAAAGTGCTCCTGCAGTACACTGGCCAC
                    ************************************************************

HsCD00444007        ATCACGTGGACACCTCCAGCCATCTTTAAAAGCTACTGTGAGATCATCGTCACCCACTTT
NM_000079.3         ATCACGTGGACACCTCCAGCCATCTTTAAAAGCTACTGTGAGATCATCGTCACCCACTTT
NM_001039523.2      ATCACGTGGACACCTCCAGCCATCTTTAAAAGCTACTGTGAGATCATCGTCACCCACTTT
                    ************************************************************

HsCD00444007        CCCTTTGATGAACAGAACTGCAGCATGAAGCTGGGCACCTGGACCTACGACGGCTCTGTC
NM_000079.3         CCCTTTGATGAACAGAACTGCAGCATGAAGCTGGGCACCTGGACCTACGACGGCTCTGTC
NM_001039523.2      CCCTTTGATGAACAGAACTGCAGCATGAAGCTGGGCACCTGGACCTACGACGGCTCTGTC
                    ************************************************************

HsCD00444007        GTGGCCATCAACCCGGAAAGCGACCAGCCAGACCTGAGCAACTTCATGGAGAGCGGGGAG
NM_000079.3         GTGGCCATCAACCCGGAAAGCGACCAGCCAGACCTGAGCAACTTCATGGAGAGCGGGGAG
NM_001039523.2      GTGGCCATCAACCCGGAAAGCGACCAGCCAGACCTGAGCAACTTCATGGAGAGCGGGGAG
                    ************************************************************

HsCD00444007        TGGGTGATCAAGGAGTCCCGGGGCTGGAAGCACTCCGTGACCTATTCCTGCTGCCCCGAC
NM_000079.3         TGGGTGATCAAGGAGTCCCGGGGCTGGAAGCACTCCGTGACCTATTCCTGCTGCCCCGAC
NM_001039523.2      TGGGTGATCAAGGAGTCCCGGGGCTGGAAGCACTCCGTGACCTATTCCTGCTGCCCCGAC
                    ************************************************************

HsCD00444007        ACCCCCTACCTGGACATCACCTACCACTTCGTCATGCAGCGCCTGCCCCTCTACTTCATC
NM_000079.3         ACCCCCTACCTGGACATCACCTACCACTTCGTCATGCAGCGCCTGCCCCTCTACTTCATC
NM_001039523.2      ACCCCCTACCTGGACATCACCTACCACTTCGTCATGCAGCGCCTGCCCCTCTACTTCATC
                    ************************************************************

HsCD00444007        GTCAACGTCATCATCCCCTGCCTGCTCTTCTCCTTCTTAACTGGCCTGGTATTCTACCTG
NM_000079.3         GTCAACGTCATCATCCCCTGCCTGCTCTTCTCCTTCTTAACTGGCCTGGTATTCTACCTG
NM_001039523.2      GTCAACGTCATCATCCCCTGCCTGCTCTTCTCCTTCTTAACTGGCCTGGTATTCTACCTG
                    ************************************************************

HsCD00444007        CCCACAGACTCAGGTGGGTGTGGTTGCCATGACTGCTGCTGC------------------
NM_000079.3         CCCACAGACTCAGGGGAGAAGATGACTCTGAGCATCTCTGTCTTACTGTCTTTGACTGTG
NM_001039523.2      CCCACAGACTCAGGGGAGAAGATGACTCTGAGCATCTCTGTCTTACTGTCTTTGACTGTG
                    ************** *.*:. .  :  *: ..*: **    *                  

HsCD00444007        ------------------------------------------------------------
NM_000079.3         TTCCTTCTGGTCATCGTGGAGCTGATCCCCTCCACGTCCAGTGCTGTGCCCTTGATTGGA
NM_001039523.2      TTCCTTCTGGTCATCGTGGAGCTGATCCCCTCCACGTCCAGTGCTGTGCCCTTGATTGGA
                                                                                

HsCD00444007        ------------------------------------------------------------
NM_000079.3         AAATACATGCTGTTCACCATGGTGTTCGTCATTGCCTCCATCATCATCACTGTCATCGTC
NM_001039523.2      AAATACATGCTGTTCACCATGGTGTTCGTCATTGCCTCCATCATCATCACTGTCATCGTC
                                                                                

HsCD00444007        ------------------------------------------------------------
NM_000079.3         ATCAACACACACCACCGCTCACCCAGCACCCATGTCATGCCCAACTGGGTGCGGAAGGTT
NM_001039523.2      ATCAACACACACCACCGCTCACCCAGCACCCATGTCATGCCCAACTGGGTGCGGAAGGTT
                                                                                

HsCD00444007        ------------------------------------------------------------
NM_000079.3         TTTATCGACACTATCCCAAATATCATGTTTTTCTCCACAATGAAAAGACCATCCAGAGAA
NM_001039523.2      TTTATCGACACTATCCCAAATATCATGTTTTTCTCCACAATGAAAAGACCATCCAGAGAA
                                                                                

HsCD00444007        ------------------------------------------------------------
NM_000079.3         AAGCAAGACAAAAAGATTTTTACAGAAGACATTGATATCTCTGACATTTCTGGAAAGCCA
NM_001039523.2      AAGCAAGACAAAAAGATTTTTACAGAAGACATTGATATCTCTGACATTTCTGGAAAGCCA
                                                                                

HsCD00444007        ------------------------------------------------------------
NM_000079.3         GGGCCTCCACCCATGGGCTTCCACTCTCCCCTGATCAAACACCCCGAGGTGAAAAGTGCC
NM_001039523.2      GGGCCTCCACCCATGGGCTTCCACTCTCCCCTGATCAAACACCCCGAGGTGAAAAGTGCC
                                                                                

HsCD00444007        ------------------------------------------------------------
NM_000079.3         ATCGAGGGCATCAAGTACATCGCAGAGACCATGAAGTCAGACCAGGAGTCTAACAATGCG
NM_001039523.2      ATCGAGGGCATCAAGTACATCGCAGAGACCATGAAGTCAGACCAGGAGTCTAACAATGCG
                                                                                

HsCD00444007        ------------------------------------------------------------
NM_000079.3         GCGGCAGAGTGGAAGTACGTTGCAATGGTGATGGACCACATACTCCTCGGAGTCTTCATG
NM_001039523.2      GCGGCAGAGTGGAAGTACGTTGCAATGGTGATGGACCACATACTCCTCGGAGTCTTCATG
                                                                                

HsCD00444007        ------------------------------------------------------------
NM_000079.3         CTTGTTTGCATCATCGGAACCCTAGCCGTGTTTGCAGGTCGACTCATTGAATTAAATCAG
NM_001039523.2      CTTGTTTGCATCATCGGAACCCTAGCCGTGTTTGCAGGTCGACTCATTGAATTAAATCAG
                                                                                

HsCD00444007        ---------
NM_000079.3         CAAGGATGA
NM_001039523.2      CAAGGATGA
                             

Pathways

NCI : Effects of Botulinum toxin
NCI : ErbB2/ErbB3 signaling events
Panther : Nicotinic acetylcholine receptor signaling pathway
Reactome : Acetylcholine Binding And Downstream Events
Reactome : Activation of Nicotinic Acetylcholine Receptors
Reactome : Highly calcium permeable nicotinic acetylcholine receptors
Reactome : Highly calcium permeable postsynaptic nicotinic acetylcholine receptors
Reactome : Neuronal System
Reactome : Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell
Reactome : Postsynaptic nicotinic acetylcholine receptors
Reactome : Presynaptic nicotinic acetylcholine receptors
Reactome : Transmission across Chemical Synapses

Protein

HPRD : 00007

Clone

Original Clone ID : ccsbBroad304_10733

Organism


TAX_ID : 9606
Species Specific ID: 1134

Genome

Chromosome : 2
Map Location : 2q31.1
Ensembl : ENSG00000138435

Reagents

Labome : CHRNA1-antibody