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PSD2 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  PSD2
Gene Name:  pleckstrin and Sec7 domain containing 2
Sequence             
Original Clone ID: ccsbBroad304_09163
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00444147

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 2314nts         Open reading frame : 1 to 2314
Reference Sequence Alignment

ATGGAGGAGGACAAGCTCTTATCTGCAGTGCCTGAGGAAGGCGATGCCACCCGTGACCCC
GGTCCAGAGCCTGAAGAGGAGCCAGGGGTCCGGAATGGGATGGCCAGTGAGGGCCTGAAC
AGCAGCCTCTGCAGCCCAGGGCACGAGCGAAGGGGCACCCCAGCGGACACTGAGGAACCC
ACGAAGGACCCAGATGTGGCCTTCCATGGCCTCAGCCTTGGCCTCTCTCTCACCAATGGC
CTAGCCCTGGGGCCAGACTTGAACATTCTGGAAGATTCAGCGGAGTCCAGGCCCTGGAGG
GCTGGCGTGCTGGCAGAGGGGGACAATGCTTCCAGGAGCCTCTACCCAGATGCTGAGGAC
CCTCAGCTGGGGTTGGATGGTCCCGGGGAGCCAGATGTGCGGGATGGCTTCAGCGCCACG
TTTGAGAAGATTCTGGAGTCAGAGCTGCTGCGGGGCACCCAGTACAGCAGCCTCGACTCC
CTAGACGGGCTGAGCCTCACGGATGAGAGCGACAGCTGCGTCAGCTTCGAGGCCCCCCTC
ACACCCCTCATCCAGCAGCGGGCCCGTGACAGCCCTGAGCCAGGGGCTGGGTTGGGCATT
GGGGACATGGCGTTTGAGGGGGACATGGGGGCGGCTGGTGGTGATGGGGAGCTGGGCAGC
CCCCTGCGGCGCTCCATCTCCAGCAGCCGCTCTGAGAATGTCCTGAGCCGCCTGTCTCTC
ATGGCCATGCCCAATGGATTCCATGAAGATGGCCCTCAGGGCCCAGGGGGGGATGAGGAT
GATGATGAGGAGGACACGGACAAGTTGCTGAACTCAGCCAGTGACCCCAGCCTGAAGGAT
GGCCTGTCAGACTCAGACTCTGAGCTCAGCAGCTCGGAGGGGTTGGAGCCTGGTAGTGCA
GACCCTCTGGCCAACGGGTGCCAGGGGGTCAGTGAAGCTGCTCATCGGCTGGCACGCCGT
CTCTACCACCTCGAGGGCTTCCAGCGCTGTGATGTGGCCCGGCAGCTGGGCAAGAACAAC
GAGTTTAGCAGGCTGGTGGCCGGGGAGTACCTCAGTTTCTTCGACTTCTCGGGCTTGACT
CTGGACGGAGCACTCAGAACATTCTTGAAGGCCTTCCCGCTGATGGGGGAGACACAAGAG
CGTGAGCGGGTCCTCACACACTTCTCCCGCCGGTACTGCCAGTGCAACCCTGATGACAGC
ACTTCGGAAGATGGGATCCACACGCTCACCTGTGCCCTGATGCTGCTCAACACGGACCTG
CACGGCCACAACATTGGCAAAAAGATGTCCTGTCAGCAATTCATTGCCAACTTGGACCAG
CTGAATGATGGCCAAGACTTTGCCAAAGACCTGCTGAAGACCCTTTACAACTCCATCAAG
AATGAAAAGCTGGAATGGGCCATTGATGAGGATGAGCTGAGGAAATCCCTGTCTGAGCTG
GTGGATGACAAGTTCGGGACAGGCACGAAGAAGGTGACGCGAATCCTGGATGGTGGCAAC
CCCTTCCTGGATGTCCCACAGGCGCTCAGTGCCACCACCTACAAGCACGGCGTCCTGACC
CGGAAGACTCACGCTGACATGGATGGCAAGAGGACGCCCCGTGGGAGGCGTGGCTGGAAG
AAATTCTACGCAGTGCTCAAAGGGACCATCCTGTACCTGCAGAAGGATGAGTACAGGCCT
GACAAAGCTCTATCGGAGGGTGACCTGAAGAACGCCATTCGCGTGCATCACGCTCTGGCC
ACCAGGGCCTCTGACTACAGCAAGAAGTCCAACGTGCTGAAGCTTAAGACAGCCGACTGG
AGGGTATTCCTCTTCCAGGCACCGAGCAAGGAAGAAATGCTGTCCTGGATCCTCAGGATC
AACCTGGTGGCAGCCATCTTCTCTGCCCCGGCCTTCCCAGCCGCTGTCAGCTCCATGAAG
AAGTTCTGTCGGCCCCTGCTGCCCTCCTGCACCACCCGCCTCTGCCAGGAGGAGCAACTG
CGGTCTCATGAGAATAAGTTGAGGCAGCTGACTGCGGAGCTGGCCGAACACAGGTGTCAC
CCAGTCGAGAGGGGCATCAAGTCCAAGGAGGCCGAGGAGTACCGGTTGAAGGAGCACTAT
CTCACCTTCGAGAAAAGCCGTTATGAGACCTATATCCACCTCCTGGCTATGAAAATCAAA
GTGGGCTCAGATGATCTGGAGCGGATTGAGGCCCGGCTGGCCACTCTGGAAGGGGATGAC
CCTTCTCTCCGGAAGACACATTCAAGCCCTGCCCTCAGCCAGGGCCATGTGACTGGCAGC
AAAACCACAAAGGATGCCACTGGGCCTGATACT


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : PSD2
Symbol Nomenclature : PSD2
Designation : 6330404E20Rik|PH and SEC7 domain-containing protein 2|pleckstrin homology and SEC7 domain-containing protein 2
Full Nomenclature : pleckstrin and Sec7 domain containing 2
GENEID : 84249
GenBank Accession : AM393868
HGNC : 19092
Vega : OTTHUMG00000129240
Target GenBank: AM393868

 

Reference Sequence Alignment

  

HsCD00444147      ATGGAGGAGGACAAGCTCTTATCTGCAGTGCCTGAGGAAGGCGATGCCACCCGTGACCCC
NM_032289.2       ATGGAGGAGGACAAGCTCTTATCTGCAGTGCCTGAGGAAGGCGATGCCACCCGTGACCCC
                  ************************************************************

HsCD00444147      GGTCCAGAGCCTGAAGAGGAGCCAGGGGTCCGGAATGGGATGGCCAGTGAGGGCCTGAAC
NM_032289.2       GGTCCAGAGCCTGAAGAGGAGCCAGGGGTCCGGAATGGGATGGCCAGTGAGGGCCTGAAC
                  ************************************************************

HsCD00444147      AGCAGCCTCTGCAGCCCAGGGCACGAGCGAAGGGGCACCCCAGCGGACACTGAGGAACCC
NM_032289.2       AGCAGCCTCTGCAGCCCAGGGCACGAGCGAAGGGGCACCCCAGCGGACACTGAGGAACCC
                  ************************************************************

HsCD00444147      ACGAAGGACCCAGATGTGGCCTTCCATGGCCTCAGCCTTGGCCTCTCTCTCACCAATGGC
NM_032289.2       ACGAAGGACCCAGATGTGGCCTTCCATGGCCTCAGCCTTGGCCTCTCTCTCACCAATGGC
                  ************************************************************

HsCD00444147      CTAGCCCTGGGGCCAGACTTGAACATTCTGGAAGATTCAGCGGAGTCCAGGCCCTGGAGG
NM_032289.2       CTAGCCCTGGGGCCAGACTTGAACATTCTGGAAGATTCAGCGGAGTCCAGGCCCTGGAGG
                  ************************************************************

HsCD00444147      GCTGGCGTGCTGGCAGAGGGGGACAATGCTTCCAGGAGCCTCTACCCAGATGCTGAGGAC
NM_032289.2       GCTGGCGTGCTGGCAGAGGGGGACAATGCTTCCAGGAGCCTCTACCCAGATGCTGAGGAC
                  ************************************************************

HsCD00444147      CCTCAGCTGGGGTTGGATGGTCCCGGGGAGCCAGATGTGCGGGATGGCTTCAGCGCCACG
NM_032289.2       CCTCAGCTGGGGTTGGATGGTCCCGGGGAGCCAGATGTGCGGGATGGCTTCAGCGCCACG
                  ************************************************************

HsCD00444147      TTTGAGAAGATTCTGGAGTCAGAGCTGCTGCGGGGCACCCAGTACAGCAGCCTCGACTCC
NM_032289.2       TTTGAGAAGATTCTGGAGTCAGAGCTGCTGCGGGGCACCCAGTACAGCAGCCTCGACTCC
                  ************************************************************

HsCD00444147      CTAGACGGGCTGAGCCTCACGGATGAGAGCGACAGCTGCGTCAGCTTCGAGGCCCCCCTC
NM_032289.2       CTAGACGGGCTGAGCCTCACGGATGAGAGCGACAGCTGCGTCAGCTTCGAGGCCCCCCTC
                  ************************************************************

HsCD00444147      ACACCCCTCATCCAGCAGCGGGCCCGTGACAGCCCTGAGCCAGGGGCTGGGTTGGGCATT
NM_032289.2       ACACCCCTCATCCAGCAGCGGGCCCGTGACAGCCCTGAGCCAGGGGCTGGGTTGGGCATT
                  ************************************************************

HsCD00444147      GGGGACATGGCGTTTGAGGGGGACATGGGGGCGGCTGGTGGTGATGGGGAGCTGGGCAGC
NM_032289.2       GGGGACATGGCGTTTGAGGGGGACATGGGGGCAGCTGGTGGTGATGGGGAGCTGGGCAGC
                  ********************************.***************************

HsCD00444147      CCCCTGCGGCGCTCCATCTCCAGCAGCCGCTCTGAGAATGTCCTGAGCCGCCTGTCTCTC
NM_032289.2       CCCCTGCGGCGCTCCATCTCCAGCAGCCGCTCTGAGAATGTCCTGAGCCGCCTGTCTCTC
                  ************************************************************

HsCD00444147      ATGGCCATGCCCAATGGATTCCATGAAGATGGCCCTCAGGGCCCAGGGGGGGATGAGGAT
NM_032289.2       ATGGCCATGCCCAATGGATTCCATGAAGATGGCCCTCAGGGCCCAGGGGGGGATGAGGAT
                  ************************************************************

HsCD00444147      GATGATGAGGAGGACACGGACAAGTTGCTGAACTCAGCCAGTGACCCCAGCCTGAAGGAT
NM_032289.2       GATGATGAGGAGGACACGGACAAGTTGCTGAACTCAGCCAGTGACCCCAGCCTGAAGGAT
                  ************************************************************

HsCD00444147      GGCCTGTCAGACTCAGACTCTGAGCTCAGCAGCTCGGAGGGGTTGGAGCCTGGTAGTGCA
NM_032289.2       GGCCTGTCAGACTCAGACTCTGAGCTCAGCAGCTCGGAGGGGTTGGAGCCTGGTAGTGCA
                  ************************************************************

HsCD00444147      GACCCTCTGGCCAACGGGTGCCAGGGGGTCAGTGAAGCTGCTCATCGGCTGGCACGCCGT
NM_032289.2       GACCCTCTGGCCAACGGGTGCCAGGGGGTCAGTGAAGCTGCTCATCGGCTGGCACGCCGT
                  ************************************************************

HsCD00444147      CTCTACCACCTCGAGGGCTTCCAGCGCTGTGATGTGGCCCGGCAGCTGGGCAAGAACAAC
NM_032289.2       CTCTACCACCTCGAGGGCTTCCAGCGCTGTGATGTGGCCCGGCAGCTGGGCAAGAACAAC
                  ************************************************************

HsCD00444147      GAGTTTAGCAGGCTGGTGGCCGGGGAGTACCTCAGTTTCTTCGACTTCTCGGGCTTGACT
NM_032289.2       GAGTTTAGCAGGCTGGTGGCCGGGGAGTACCTCAGTTTCTTCGACTTCTCGGGCTTGACT
                  ************************************************************

HsCD00444147      CTGGACGGAGCACTCAGAACATTCTTGAAGGCCTTCCCGCTGATGGGGGAGACACAAGAG
NM_032289.2       CTGGACGGAGCACTCAGAACATTCTTGAAGGCCTTCCCGCTGATGGGGGAGACACAAGAG
                  ************************************************************

HsCD00444147      CGTGAGCGGGTCCTCACACACTTCTCCCGCCGGTACTGCCAGTGCAACCCTGATGACAGC
NM_032289.2       CGTGAGCGGGTCCTCACACACTTCTCCCGCCGGTACTGCCAGTGCAACCCTGATGACAGC
                  ************************************************************

HsCD00444147      ACTTCGGAAGATGGGATCCACACGCTCACCTGTGCCCTGATGCTGCTCAACACGGACCTG
NM_032289.2       ACTTCGGAAGATGGGATCCACACGCTCACCTGTGCCCTGATGCTGCTCAACACGGACCTG
                  ************************************************************

HsCD00444147      CACGGCCACAACATTGGCAAAAAGATGTCCTGTCAGCAATTCATTGCCAACTTGGACCAG
NM_032289.2       CACGGCCACAACATTGGCAAAAAGATGTCCTGTCAGCAATTCATTGCCAACTTGGACCAG
                  ************************************************************

HsCD00444147      CTGAATGATGGCCAAGACTTTGCCAAAGACCTGCTGAAGACCCTTTACAACTCCATCAAG
NM_032289.2       CTGAATGATGGCCAAGACTTTGCCAAAGACCTGCTGAAGACCCTTTACAACTCCATCAAG
                  ************************************************************

HsCD00444147      AATGAAAAGCTGGAATGGGCCATTGATGAGGATGAGCTGAGGAAATCCCTGTCTGAGCTG
NM_032289.2       AATGAAAAGCTGGAATGGGCCATTGATGAGGATGAGCTGAGGAAATCCCTGTCTGAGCTG
                  ************************************************************

HsCD00444147      GTGGATGACAAGTTCGGGACAGGCACGAAGAAGGTGACGCGAATCCTGGATGGTGGCAAC
NM_032289.2       GTGGATGACAAGTTCGGGACAGGCACGAAGAAGGTGACGCGAATCCTGGATGGTGGCAAC
                  ************************************************************

HsCD00444147      CCCTTCCTGGATGTCCCACAGGCGCTCAGTGCCACCACCTACAAGCACGGCGTCCTGACC
NM_032289.2       CCCTTCCTGGATGTCCCACAGGCGCTCAGTGCCACCACCTACAAGCACGGCGTCCTGACC
                  ************************************************************

HsCD00444147      CGGAAGACTCACGCTGACATGGATGGCAAGAGGACGCCCCGTGGGAGGCGTGGCTGGAAG
NM_032289.2       CGGAAGACTCACGCTGACATGGATGGCAAGAGGACGCCCCGTGGGAGGCGTGGCTGGAAG
                  ************************************************************

HsCD00444147      AAATTCTACGCAGTGCTCAAAGGGACCATCCTGTACCTGCAGAAGGATGAGTACAGGCCT
NM_032289.2       AAATTCTACGCAGTGCTCAAAGGGACCATCCTGTACCTGCAGAAGGATGAGTACAGGCCT
                  ************************************************************

HsCD00444147      GACAAAGCTCTATCGGAGGGTGACCTGAAGAACGCCATTCGCGTGCATCACGCTCTGGCC
NM_032289.2       GACAAAGCTCTATCGGAGGGTGACCTGAAGAACGCCATTCGCGTGCATCACGCTCTGGCC
                  ************************************************************

HsCD00444147      ACCAGGGCCTCTGACTACAGCAAGAAGTCCAACGTGCTGAAGCTTAAGACAGCCGACTGG
NM_032289.2       ACCAGGGCCTCTGACTACAGCAAGAAGTCCAACGTGCTGAAGCTTAAGACAGCCGACTGG
                  ************************************************************

HsCD00444147      AGGGTATTCCTCTTCCAGGCACCGAGCAAGGAAGAAATGCTGTCCTGGATCCTCAGGATC
NM_032289.2       AGGGTATTCCTCTTCCAGGCACCGAGCAAGGAAGAAATGCTGTCCTGGATCCTCAGGATC
                  ************************************************************

HsCD00444147      AACCTGGTGGCAGCCATCTTCTCTGCCCCGGCCTTCCCAGCCGCTGTCAGCTCCATGAAG
NM_032289.2       AACCTGGTGGCAGCCATCTTCTCTGCCCCGGCCTTCCCAGCCGCTGTCAGCTCCATGAAG
                  ************************************************************

HsCD00444147      AAGTTCTGTCGGCCCCTGCTGCCCTCCTGCACCACCCGCCTCTGCCAGGAGGAGCAACTG
NM_032289.2       AAGTTCTGTCGGCCCCTGCTGCCCTCCTGCACCACCCGCCTCTGCCAGGAGGAGCAACTG
                  ************************************************************

HsCD00444147      CGGTCTCATGAGAATAAGTTGAGGCAGCTGACTGCGGAGCTGGCCGAACACAGGTGTCAC
NM_032289.2       CGGTCTCATGAGAATAAGTTGAGGCAGCTGACTGCGGAGCTGGCCGAACACAGGTGTCAC
                  ************************************************************

HsCD00444147      CCAGTCGAGAGGGGCATCAAGTCCAAGGAGGCCGAGGAGTACCGGTTGAAGGAGCACTAT
NM_032289.2       CCAGTCGAGAGGGGCATCAAGTCCAAGGAGGCCGAGGAGTACCGGTTGAAGGAGCACTAT
                  ************************************************************

HsCD00444147      CTCACCTTCGAGAAAAGCCGTTATGAGACCTATATCCACCTCCTGGCTATGAAAATCAAA
NM_032289.2       CTCACCTTCGAGAAAAGCCGTTATGAGACCTATATCCACCTCCTGGCTATGAAAATCAAA
                  ************************************************************

HsCD00444147      GTGGGCTCAGATGATCTGGAGCGGATTGAGGCCCGGCTGGCCACTCTGGAAGGGGATGAC
NM_032289.2       GTGGGCTCAGATGATCTGGAGCGGATTGAGGCCCGGCTGGCCACTCTGGAAGGGGATGAC
                  ************************************************************

HsCD00444147      CCTTCTCTCCGGAAGACACATTCAAGCCCTGCCCTCAGCCAGGGCCATGTGACTGGCAGC
NM_032289.2       CCTTCTCTCCGGAAGACACATTCAAGCCCTGCCCTCAGCCAGGGCCATGTGACTGGCAGC
                  ************************************************************

HsCD00444147      AAAACCACAAAGGATGCCACTGGGCCTGATACT---
NM_032289.2       AAAACCACAAAGGATGCCACTGGGCCTGATACTTAG
                  *********************************   

Protein

SMART domain : PH : Pleckstrin homology domain.
SMART domain : Sec7 : Sec7 domain
HPRD : 15190

Clone

Original Clone ID : ccsbBroad304_09163

Organism


TAX_ID : 9606
Species Specific ID: 84249

Genome

Chromosome : 5
Map Location : 5q31.2
Ensembl : ENSG00000146005

Reagents

Labome : PSD2-antibody