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THOC1 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  THOC1
Gene Name:  THO complex 1
Sequence             
Original Clone ID: ccsbBroad304_07525
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00444461

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1972nts         Open reading frame : 1 to 1972
Reference Sequence Alignment

ATGTCTCCGACGCCGCCGCTCTTCAGTTTGCCCGAAGCGCGGACGCGGTTTACGAAGTCT
ACCAGAGAGGCCTTGAACAACAAAAACATCAAGCCATTGTTAAGTACCTTCAGCCAGGTA
CCTGGCAGTGAAAATGAAAAAAAATGTACCCTTGACCAAGCTTTCAGAGGTATTCTAGAA
GAAGAAATTATAAATCATTCATCATGTGAAAACGTTTTAGCTATTATTTCTCTTGCTATT
GGGGGAGTAACTGAAGGTATTTGTACCGCATCTACACCTTTTGTATTGTTGGGAGATGTT
TTGGATTGTCTTCCTTTGGATCAGTGTGACACAATATTCACTTTTGTGGAAAAAAATGTT
GCTACTTGGAAATCAAATACATTCTATTCTGCTGGGAAAAATTACTTACTACGTATGTGC
AATGATCTCCTAAGAAGATTGTCTAAATCCCAGAATACAGTCTTCTGTGGACGGATTCAG
CTCTTTTTGGCCAGGCTTTTCCCTCTGTCTGAGAAATCAGGTCTTAACTTGCAGAGTCAG
TTTAATCTGGAAAATGTCACTGTTTTCAATACAAATGAGCAGGAAAGCACCCTGGGTCAG
AAGCACACTGAAGATAGAGAAGAAGGAATGGATGTAGAAGAAGGCGAAATGGGAGATGAG
GAAGCTCCAACAACGTGCTCTATTCCAATTGATTACAACCTGTATCGAAAATTCTGGTCA
CTTCAGGATTACTTCAGGAACCCTGTGCAATGCTATGAGAAGATTTCATGGAAAACTTTT
CTCAAGTATTCTGAAGAAGTTTTAGCTGTTTTTAAGAGTTATAAATTAGATGATACTCAG
GCCTCAAGAAAAAAGATGGAAGAATTGAAAACAGGAGGAGAACATGTATATTTTGCAAAA
TTTTTAACAAGTGAAAAGCTGATGGATTTACAACTGAGTGACAGTAACTTTCGTCGACAC
ATCCTGTTGCAGTATCTCATTTTATTCCAATATCTCAAGGGGCAGGTCAAATTCAAAAGT
TCAAACTATGTTTTAACTGATGAGCAATCACTTTGGATTGAAGATACTACAAAATCAGTT
TATCAACTACTATCTGAAAACCCCCCCGATGGAGAAAGATTTTCAAAGATGGTAGAGCAT
ATATTAAACACTGAAGAAAACTGGAACTCGTGGAAAAATGAAGGTTGCCCAAGTTTTGTG
AAAGAAAGAACATCAGATACCAAACCTACGAGAATAATTCGGAAGAGAACAGCACCCGAG
GACTTCCTAGGGAAAGGACCCACCAAAAAAATTCTGATGGGAAATGAGGAGTTAACAAGG
CTTTGGAATCTTTGCCCTGATAATATGGAAGCCTGTAAATCAGAGACAAGGGAACACATG
CCCACTTTGGAGGAATTCTTTGAAGAAGCCATTGAACAGGCAGACCCTGAAAATATGGTG
GAAAATGAATATAAGGCTGTGAACAATTCAAATTATGGTTGGAGAGCCCTGAGACTATTA
GCACGGAGAAGCCCTCACTTCTTCCAGCCAACCAACCAGCAGTTTAAAAGTTTACCAGAA
TATCTTGAAAATATGGTAATAAAGCTAGCCAAGGAATTACCGCCTCCTTCTGAAGAAATA
AAAACAGGTGAGGATGAAGATGAGGAAGATAATGATGCTCTACTGAAGGAAAATGAAAGT
CCTGATGTTCGGCGAGACAAACCTGTAACAGGAGAACAAATAGAGGTATTTGCCAACAAG
CTGGGTGAACAATGGAAGATTCTGGCTCCCTACTTGGAAATGAAAGACTCAGAAATTAGG
CAGATTGAGTGTGACAGTGAAGACATGAAGATGAGAGCTAAGCAGCTCCTGGTTGCCTGG
CAAGATCAAGAGGGAGTTCATGCAACACCTGAGAATCTGATTAATGCACTGAATAAGTCT
GGATTAAGTGACCTTGCAGAAAGTCTAACTAATGACAATGAGACAAATAGT


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : THOC1
Symbol Nomenclature : THOC1
SYNONYM : HPR1; P84; P84N5
Designation : THO complex subunit 1|hTREX84|nuclear matrix protein p84|tho1
Full Nomenclature : THO complex 1
GENEID : 9984
GenBank Accession : EU176747
HGNC : 19070
MIM : 606930
Vega : OTTHUMG00000178051
Target GenBank: EU176747

 

Reference Sequence Alignment

  

HsCD00444461      ATGTCTCCGACGCCGCCGCTCTTCAGTTTGCCCGAAGCGCGGACGCGGTTTACGAAGTCT
NM_005131.2       ATGTCTCCGACGCCGCCGCTCTTCAGTTTGCCCGAAGCGCGGACGCGGTTTACGAAGTCT
                  ************************************************************

HsCD00444461      ACCAGAGAGGCCTTGAACAACAAAAACATCAAGCCATTGTTAAGTACCTTCAGCCAGGTA
NM_005131.2       ACCAGAGAGGCCTTGAACAACAAAAACATCAAGCCATTGTTAAGTACCTTCAGCCAGGTA
                  ************************************************************

HsCD00444461      CCTGGCAGTGAAAATGAAAAAAAATGTACCCTTGACCAAGCTTTCAGAGGTATTCTAGAA
NM_005131.2       CCTGGCAGTGAAAATGAAAAAAAATGTACCCTTGACCAAGCTTTCAGAGGTATTCTAGAA
                  ************************************************************

HsCD00444461      GAAGAAATTATAAATCATTCATCATGTGAAAACGTTTTAGCTATTATTTCTCTTGCTATT
NM_005131.2       GAAGAAATTATAAATCATTCATCATGTGAAAACGTTTTAGCTATTATTTCTCTTGCTATT
                  ************************************************************

HsCD00444461      GGGGGAGTAACTGAAGGTATTTGTACCGCATCTACACCTTTTGTATTGTTGGGAGATGTT
NM_005131.2       GGGGGAGTAACTGAAGGTATTTGTACCGCATCTACACCTTTTGTATTGTTGGGAGATGTT
                  ************************************************************

HsCD00444461      TTGGATTGTCTTCCTTTGGATCAGTGTGACACAATATTCACTTTTGTGGAAAAAAATGTT
NM_005131.2       TTGGATTGTCTTCCTTTGGATCAGTGTGACACAATATTCACTTTTGTGGAAAAAAATGTT
                  ************************************************************

HsCD00444461      GCTACTTGGAAATCAAATACATTCTATTCTGCTGGGAAAAATTACTTACTACGTATGTGC
NM_005131.2       GCTACTTGGAAATCAAATACATTCTATTCTGCTGGGAAAAATTACTTACTACGTATGTGC
                  ************************************************************

HsCD00444461      AATGATCTCCTAAGAAGATTGTCTAAATCCCAGAATACAGTCTTCTGTGGACGGATTCAG
NM_005131.2       AATGATCTCCTAAGAAGATTGTCTAAATCCCAGAATACAGTCTTCTGTGGACGGATTCAG
                  ************************************************************

HsCD00444461      CTCTTTTTGGCCAGGCTTTTCCCTCTGTCTGAGAAATCAGGTCTTAACTTGCAGAGTCAG
NM_005131.2       CTCTTTTTGGCCAGGCTTTTCCCTCTGTCTGAGAAATCAGGTCTTAACTTGCAGAGTCAG
                  ************************************************************

HsCD00444461      TTTAATCTGGAAAATGTCACTGTTTTCAATACAAATGAGCAGGAAAGCACCCTGGGTCAG
NM_005131.2       TTTAATCTGGAAAATGTCACTGTTTTCAATACAAATGAGCAGGAAAGCACCCTGGGTCAG
                  ************************************************************

HsCD00444461      AAGCACACTGAAGATAGAGAAGAAGGAATGGATGTAGAAGAAGGCGAAATGGGAGATGAG
NM_005131.2       AAGCACACTGAAGATAGAGAAGAAGGAATGGATGTAGAAGAAGGCGAAATGGGAGACGAG
                  ******************************************************** ***

HsCD00444461      GAAGCTCCAACAACGTGCTCTATTCCAATTGATTACAACCTGTATCGAAAATTCTGGTCA
NM_005131.2       GAAGCTCCAACAACGTGCTCTATTCCAATTGATTACAACCTGTATCGAAAATTCTGGTCA
                  ************************************************************

HsCD00444461      CTTCAGGATTACTTCAGGAACCCTGTGCAATGCTATGAGAAGATTTCATGGAAAACTTTT
NM_005131.2       CTTCAGGATTACTTCAGGAACCCTGTGCAATGCTATGAGAAGATTTCATGGAAAACTTTT
                  ************************************************************

HsCD00444461      CTCAAGTATTCTGAAGAAGTTTTAGCTGTTTTTAAGAGTTATAAATTAGATGATACTCAG
NM_005131.2       CTCAAGTATTCTGAAGAAGTTTTAGCTGTTTTTAAGAGTTATAAATTAGATGATACTCAG
                  ************************************************************

HsCD00444461      GCCTCAAGAAAAAAGATGGAAGAATTGAAAACAGGAGGAGAACATGTATATTTTGCAAAA
NM_005131.2       GCCTCAAGAAAAAAGATGGAAGAATTGAAAACAGGAGGAGAACATGTATATTTTGCAAAA
                  ************************************************************

HsCD00444461      TTTTTAACAAGTGAAAAGCTGATGGATTTACAACTGAGTGACAGTAACTTTCGTCGACAC
NM_005131.2       TTTTTAACAAGTGAAAAGCTGATGGATTTACAACTGAGTGACAGTAACTTTCGTCGACAC
                  ************************************************************

HsCD00444461      ATCCTGTTGCAGTATCTCATTTTATTCCAATATCTCAAGGGGCAGGTCAAATTCAAAAGT
NM_005131.2       ATCCTGTTGCAGTATCTCATTTTATTCCAATATCTCAAGGGGCAGGTCAAATTCAAAAGT
                  ************************************************************

HsCD00444461      TCAAACTATGTTTTAACTGATGAGCAATCACTTTGGATTGAAGATACTACAAAATCAGTT
NM_005131.2       TCAAACTATGTTTTAACTGATGAGCAATCACTTTGGATTGAAGATACTACAAAATCAGTT
                  ************************************************************

HsCD00444461      TATCAACTACTATCTGAAAACCCCCCCGATGGAGAAAGATTTTCAAAGATGGTAGAGCAT
NM_005131.2       TATCAACTACTATCTGAAAACCCCCCCGATGGAGAAAGATTTTCAAAGATGGTAGAGCAT
                  ************************************************************

HsCD00444461      ATATTAAACACTGAAGAAAACTGGAACTCGTGGAAAAATGAAGGTTGCCCAAGTTTTGTG
NM_005131.2       ATATTAAACACTGAAGAAAACTGGAACTCGTGGAAAAATGAAGGTTGCCCAAGTTTTGTG
                  ************************************************************

HsCD00444461      AAAGAAAGAACATCAGATACCAAACCTACGAGAATAATTCGGAAGAGAACAGCACCCGAG
NM_005131.2       AAAGAAAGAACATCAGATACCAAACCTACGAGAATAATTCGGAAGAGAACAGCACCCGAG
                  ************************************************************

HsCD00444461      GACTTCCTAGGGAAAGGACCCACCAAAAAAATTCTGATGGGAAATGAGGAGTTAACAAGG
NM_005131.2       GACTTCCTAGGGAAAGGACCCACCAAAAAAATTCTGATGGGAAATGAGGAGTTAACAAGG
                  ************************************************************

HsCD00444461      CTTTGGAATCTTTGCCCTGATAATATGGAAGCCTGTAAATCAGAGACAAGGGAACACATG
NM_005131.2       CTTTGGAATCTTTGCCCTGATAATATGGAAGCCTGTAAATCAGAGACAAGGGAACACATG
                  ************************************************************

HsCD00444461      CCCACTTTGGAGGAATTCTTTGAAGAAGCCATTGAACAGGCAGACCCTGAAAATATGGTG
NM_005131.2       CCCACTTTGGAGGAATTCTTTGAAGAAGCCATTGAACAGGCAGACCCTGAAAATATGGTG
                  ************************************************************

HsCD00444461      GAAAATGAATATAAGGCTGTGAACAATTCAAATTATGGTTGGAGAGCCCTGAGACTATTA
NM_005131.2       GAAAATGAATATAAGGCTGTGAACAATTCAAATTATGGTTGGAGAGCCCTGAGACTATTA
                  ************************************************************

HsCD00444461      GCACGGAGAAGCCCTCACTTCTTCCAGCCAACCAACCAGCAGTTTAAAAGTTTACCAGAA
NM_005131.2       GCACGGAGAAGCCCTCACTTCTTCCAGCCAACCAACCAGCAGTTTAAAAGTTTACCAGAA
                  ************************************************************

HsCD00444461      TATCTTGAAAATATGGTAATAAAGCTAGCCAAGGAATTACCGCCTCCTTCTGAAGAAATA
NM_005131.2       TATCTTGAAAATATGGTAATAAAGCTAGCCAAGGAATTACCGCCTCCTTCTGAAGAAATA
                  ************************************************************

HsCD00444461      AAAACAGGTGAGGATGAAGATGAGGAAGATAATGATGCTCTACTGAAGGAAAATGAAAGT
NM_005131.2       AAAACAGGTGAGGATGAAGATGAGGAAGATAATGATGCTCTACTGAAGGAAAATGAAAGT
                  ************************************************************

HsCD00444461      CCTGATGTTCGGCGAGACAAACCTGTAACAGGAGAACAAATAGAGGTATTTGCCAACAAG
NM_005131.2       CCTGATGTTCGGCGAGACAAACCTGTAACAGGAGAACAAATAGAGGTATTTGCCAACAAG
                  ************************************************************

HsCD00444461      CTGGGTGAACAATGGAAGATTCTGGCTCCCTACTTGGAAATGAAAGACTCAGAAATTAGG
NM_005131.2       CTGGGTGAACAATGGAAGATTCTGGCTCCCTACTTGGAAATGAAAGACTCAGAAATTAGG
                  ************************************************************

HsCD00444461      CAGATTGAGTGTGACAGTGAAGACATGAAGATGAGAGCTAAGCAGCTCCTGGTTGCCTGG
NM_005131.2       CAGATTGAGTGTGACAGTGAAGACATGAAGATGAGAGCTAAGCAGCTCCTGGTTGCCTGG
                  ************************************************************

HsCD00444461      CAAGATCAAGAGGGAGTTCATGCAACACCTGAGAATCTGATTAATGCACTGAATAAGTCT
NM_005131.2       CAAGATCAAGAGGGAGTTCATGCAACACCTGAGAATCTGATTAATGCACTGAATAAGTCT
                  ************************************************************

HsCD00444461      GGATTAAGTGACCTTGCAGAAAGTCTAACTAATGACAATGAGACAAATAGT---
NM_005131.2       GGATTAAGTGACCTTGCAGAAAGTCTAACTAATGACAATGAGACAAATAGTTAG
                  ***************************************************   

Clone

Original Clone ID : ccsbBroad304_07525

Organism


TAX_ID : 9606
Species Specific ID: 9984

Genome

Chromosome : 18
Map Location : 18p11.32
Ensembl : ENSG00000079134

Reagents

Labome : THOC1-antibody