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LRRC45 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  LRRC45
Gene Name:  leucine rich repeat containing 45
Sequence             
Original Clone ID: ccsbBroad304_05197
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00444478

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 2011nts         Open reading frame : 1 to 2011
Reference Sequence Alignment

ATGGAGGAGTTCCGGCGCTCCTACAGCCGCCTGTGCAGGGAGAGTGGGGCCGAGCCCCAG
GAGGCTGTCCTGCAGCAGCTGCACCAGCTTCCCAGGGGCCGGCTGGACCTGGCCACGCAA
AGCCTGACGGTGGAGACCTGCAGGGCCCTGGGCAAGCTGCTGCCGAGGGAGACGCTGTGC
ACGGAGCTGGTCCTGAGTGACTGCATGCTCAGCGAGGAAGGGGCCACACTGCTGCTCCGA
GGCCTGTGTGCCAACACCGTGCTGCGCTTTCTGGACTTAAAGGGCAACAACCTTCGGGCT
GCAGGGGCCGAGGCTCTGGGAAAACTCCTCCAACAGAACAAGTCCATTCAGAGCCTCACG
CTGGAGTGGAACAGCCTGGGCACGTGGGACGATGCCTTCGCCACCTTCTGCGGGGGCCTG
GCGGCCAACGGCGCCCTGCAGCGGCTGGACCTCCGCAACAACCAGATCAGTCACAAGGGC
GCGGAGGAGCTGGCCCTAGCCCTGAAGGGCAACACCACCCTCCAGCAGCTGGACCTGCGC
TGGAATAACGTTGGCCTCCTGGGGGGCCGGGCCCTCATGAACTGTCTCCCCAGCAACAGA
ACCCTGTGGAGACTGGACCTGGCTGGGAACAACATCCCTGGAGACGTCCTCAGAGCCGTG
GAGCAAGCCATGGGCCACAGCCAGGACCGGCTCACCACCTTCCAGGAGAACCAAGCCCGC
ACTCACGTCCTCAGCAAGGAGGTCCAGCACCTCCGGGAGGAGAAGTCCAAGCAGTTCCTC
GACTTGATGGAGACTATTGATAAGCAGCGAGAAGAGATGGCCAAGAGCAGCAGGGCGTCG
GCAGCCCGTGTAGGGCAGCTTCAGGAAGCCCTGAATGAGAGGCACTCCATCATCAACGCT
CTCAAGGCCAAGCTGCAGATGACAGAGGCCGCCCTGGCTCTGTCGGAGCAGAAGGCCCAG
GACCTGGGGGAGCTCCTGGCCACAGCGGAGCAGGAGCAGCTGAGCCTGTCACAGAGGCAG
GCCAAGGAGCTCAAGCTGGAGCAGCAGGAAGCTGCAGAGCGGGAGTCTAAACTCCTCAGA
GACTTGTCTGCTGCCAATGAAAAGAACCTGCTTCTGCAAAACCAGGTAGACGAGTTGGAG
CGGAAGTTCAGGTGTCAGCAGGAGCAGCTGTTCCAGACCAGGCAGGAGATGACCAGCATG
TCAGCTGAGCTGAAGATGCGGGCCATCCAGGCCGAGGAGCGCCTGGACATGGAGAAGAGA
AGATGCAGACAGAGCCTGGAGGACTCCGAAAGCCTGCGCATCAAGGAGGTGGAGCATATG
ACCCGTCACCTGGAGGAGAGTGAGAAGGCCATGCAGGAGCGGGTGCAGAGGCTGGAGGCG
GCGCGGCTGTCCCTGGAGGAGGAGCTGAGCCGAGTGAAAGCAGCGGCACTCAGCGAGCGT
GGCCAGGCTGAGGAGGAGCTGATCAAGGCCAAGAGCCAGGCCCGCCTGGAGGAGCAACAG
CGCCTGGCTCACCTGGAGGACAAGCTGAGACTGCTGGCGCAGGCACGGGACGAGGCGCAG
GGCGCTTGCCTACAGCAGAAGCAGGTGGTGGCCGAGGCCCAGACCCGGGTCAGCCAGCTG
GGCCTGCAAGTTGAGGGCCTGCGGCGGCGCCTGGAAGAGCTGCAGCAGGAGCTGAGCCTC
AAGGACCAGGAAAGGGTGGCCGAGGTGAGCAGGGTGCGCGTGGAGCTGCAGGAGCAGAAC
GGCCGGCTGCAGGCGGAGCTGGCGGCTCAGGAGGCGCTGAGGGAGAAGGCGGCGGCCCTG
GAGCGCCAGCTGAAAGTGATGGCGAGCGACCACCGAGAGGCGCTGCTGGACAGGGAGAGC
GAGAACGCGTCTCTCCGGGAGAAGCTGCGGCTCCGGGAGGCGGAGATCGCCCGCATCCGG
GACGAGGAGGCCCAGAGGGCGAGCTTCCTGCAGAACGCCGTCCTGGCTTACGTGCAGGCG
TCCCCCGTGAGGACCCTGAGCCCCCCAAAG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : LRRC45
Symbol Nomenclature : LRRC45
Designation : leucine-rich repeat-containing protein 45
Full Nomenclature : leucine rich repeat containing 45
GENEID : 201255
GenBank Accession : JF432130
HGNC : 28302
Vega : OTTHUMG00000178454
Target GenBank: JF432130

 

Reference Sequence Alignment

  

HsCD00444478      ATGGAGGAGTTCCGGCGCTCCTACAGCCGCCTGTGCAGGGAGAGTGGGGCCGAGCCCCAG
NM_144999.2       ATGGAGGAGTTCCGGCGCTCCTACAGCCGCCTGTGCAGGGAGAGTGGGGCCGAGCCCCAG
                  ************************************************************

HsCD00444478      GAGGCTGTCCTGCAGCAGCTGCACCAGCTTCCCAGGGGCCGGCTGGACCTGGCCACGCAA
NM_144999.2       GAGGCTGTCCTGCAGCAGCTGCACCAGCTTCCCAGGGGCCGGCTGGACCTGGCCACGCAA
                  ************************************************************

HsCD00444478      AGCCTGACGGTGGAGACCTGCAGGGCCCTGGGCAAGCTGCTGCCGAGGGAGACGCTGTGC
NM_144999.2       AGCCTGACGGTGGAGACCTGCAGGGCCCTGGGCAAGCTGCTGCCGAGGGAGACGCTGTGC
                  ************************************************************

HsCD00444478      ACGGAGCTGGTCCTGAGTGACTGCATGCTCAGCGAGGAAGGGGCCACACTGCTGCTCCGA
NM_144999.2       ACGGAGCTGGTCCTGAGTGACTGCATGCTCAGCGAGGAAGGGGCCACACTGCTGCTCCGA
                  ************************************************************

HsCD00444478      GGCCTGTGTGCCAACACCGTGCTGCGCTTTCTGGACTTAAAGGGCAACAACCTTCGGGCT
NM_144999.2       GGCCTGTGTGCCAACACCGTGCTGCGCTTTCTGGACTTAAAGGGCAACAACCTTCGGGCT
                  ************************************************************

HsCD00444478      GCAGGGGCCGAGGCTCTGGGAAAACTCCTCCAACAGAACAAGTCCATTCAGAGCCTCACG
NM_144999.2       GCAGGGGCCGAGGCTCTGGGAAAACTCCTCCAACAGAACAAGTCCATTCAGAGCCTCACG
                  ************************************************************

HsCD00444478      CTGGAGTGGAACAGCCTGGGCACGTGGGACGATGCCTTCGCCACCTTCTGCGGGGGCCTG
NM_144999.2       CTGGAGTGGAACAGCCTGGGCACGTGGGACGATGCCTTCGCCACCTTCTGCGGGGGCCTG
                  ************************************************************

HsCD00444478      GCGGCCAACGGCGCCCTGCAGCGGCTGGACCTCCGCAACAACCAGATCAGTCACAAGGGC
NM_144999.2       GCGGCCAACGGCGCCCTGCAGCGGCTGGACCTCCGCAACAACCAGATCAGTCACAAGGGC
                  ************************************************************

HsCD00444478      GCGGAGGAGCTGGCCCTAGCCCTGAAGGGCAACACCACCCTCCAGCAGCTGGACCTGCGC
NM_144999.2       GCGGAGGAGCTGGCCCTAGCCCTGAAGGGCAACACCACCCTCCAGCAGCTGGACCTGCGC
                  ************************************************************

HsCD00444478      TGGAATAACGTTGGCCTCCTGGGGGGCCGGGCCCTCATGAACTGTCTCCCCAGCAACAGA
NM_144999.2       TGGAATAACGTTGGCCTCCTGGGGGGCCGGGCCCTCATGAACTGTCTCCCCAGCAACAGA
                  ************************************************************

HsCD00444478      ACCCTGTGGAGACTGGACCTGGCTGGGAACAACATCCCTGGAGACGTCCTCAGAGCCGTG
NM_144999.2       ACCCTGTGGAGACTGGACCTGGCTGGGAACAACATCCCTGGAGACGTCCTCAGAGCCGTG
                  ************************************************************

HsCD00444478      GAGCAAGCCATGGGCCACAGCCAGGACCGGCTCACCACCTTCCAGGAGAACCAAGCCCGC
NM_144999.2       GAGCAAGCCATGGGCCACAGCCAGGACCGGCTCACCACCTTCCAGGAGAACCAAGCCCGC
                  ************************************************************

HsCD00444478      ACTCACGTCCTCAGCAAGGAGGTCCAGCACCTCCGGGAGGAGAAGTCCAAGCAGTTCCTC
NM_144999.2       ACTCACGTCCTCAGCAAGGAGGTCCAGCACCTCCGGGAGGAGAAGTCCAAGCAGTTCCTC
                  ************************************************************

HsCD00444478      GACTTGATGGAGACTATTGATAAGCAGCGAGAAGAGATGGCCAAGAGCAGCAGGGCGTCG
NM_144999.2       GACTTGATGGAGACTATTGATAAGCAGCGAGAAGAGATGGCCAAGAGCAGCAGGGCGTCG
                  ************************************************************

HsCD00444478      GCAGCCCGTGTAGGGCAGCTTCAGGAAGCCCTGAATGAGAGGCACTCCATCATCAACGCT
NM_144999.2       GCAGCCCGTGTAGGGCAGCTTCAGGAAGCCCTGAATGAGAGGCACTCCATCATCAACGCT
                  ************************************************************

HsCD00444478      CTCAAGGCCAAGCTGCAGATGACAGAGGCCGCCCTGGCTCTGTCGGAGCAGAAGGCCCAG
NM_144999.2       CTCAAGGCCAAGCTGCAGATGACAGAGGCCGCCCTGGCTCTGTCGGAGCAGAAGGCCCAG
                  ************************************************************

HsCD00444478      GACCTGGGGGAGCTCCTGGCCACAGCGGAGCAGGAGCAGCTGAGCCTGTCACAGAGGCAG
NM_144999.2       GACCTGGGGGAGCTCCTGGCCACAGCGGAGCAGGAGCAGCTGAGCCTGTCACAGAGGCAG
                  ************************************************************

HsCD00444478      GCCAAGGAGCTCAAGCTGGAGCAGCAGGAAGCTGCAGAGCGGGAGTCTAAACTCCTCAGA
NM_144999.2       GCCAAGGAGCTCAAGCTGGAGCAGCAGGAAGCTGCAGAGCGGGAGTCTAAACTCCTCAGA
                  ************************************************************

HsCD00444478      GACTTGTCTGCTGCCAATGAAAAGAACCTGCTTCTGCAAAACCAGGTAGACGAGTTGGAG
NM_144999.2       GACTTGTCTGCTGCCAATGAAAAGAACCTGCTTCTGCAAAACCAGGTAGACGAGTTGGAG
                  ************************************************************

HsCD00444478      CGGAAGTTCAGGTGTCAGCAGGAGCAGCTGTTCCAGACCAGGCAGGAGATGACCAGCATG
NM_144999.2       CGGAAGTTCAGGTGTCAGCAGGAGCAGCTGTTCCAGACCAGGCAGGAGATGACCAGCATG
                  ************************************************************

HsCD00444478      TCAGCTGAGCTGAAGATGCGGGCCATCCAGGCCGAGGAGCGCCTGGACATGGAGAAGAGA
NM_144999.2       TCAGCTGAGCTGAAGATGCGGGCCATCCAGGCCGAGGAGCGCCTGGACATGGAGAAGAGA
                  ************************************************************

HsCD00444478      AGATGCAGACAGAGCCTGGAGGACTCCGAAAGCCTGCGCATCAAGGAGGTGGAGCATATG
NM_144999.2       AGATGCAGACAGAGCCTGGAGGACTCCGAAAGCCTGCGCATCAAGGAGGTGGAGCATATG
                  ************************************************************

HsCD00444478      ACCCGTCACCTGGAGGAGAGTGAGAAGGCCATGCAGGAGCGGGTGCAGAGGCTGGAGGCG
NM_144999.2       ACCCGTCACCTGGAGGAGAGTGAGAAGGCCATGCAGGAGCGGGTGCAGAGGCTGGAGGCG
                  ************************************************************

HsCD00444478      GCGCGGCTGTCCCTGGAGGAGGAGCTGAGCCGAGTGAAAGCAGCGGCACTCAGCGAGCGT
NM_144999.2       GCGCGGCTGTCCCTGGAGGAGGAGCTGAGCCGAGTGAAAGCAGCGGCACTCAGCGAGCGT
                  ************************************************************

HsCD00444478      GGCCAGGCTGAGGAGGAGCTGATCAAGGCCAAGAGCCAGGCCCGCCTGGAGGAGCAACAG
NM_144999.2       GGCCAGGCTGAGGAGGAGCTGATCAAGGCCAAGAGCCAGGCCCGCCTGGAGGAGCAACAG
                  ************************************************************

HsCD00444478      CGCCTGGCTCACCTGGAGGACAAGCTGAGACTGCTGGCGCAGGCACGGGACGAGGCGCAG
NM_144999.2       CGCCTGGCTCACCTGGAGGACAAGCTGAGACTGCTGGCGCAGGCACGGGACGAGGCGCAG
                  ************************************************************

HsCD00444478      GGCGCTTGCCTACAGCAGAAGCAGGTGGTGGCCGAGGCCCAGACCCGGGTCAGCCAGCTG
NM_144999.2       GGCGCTTGCCTACAGCAGAAGCAGGTGGTGGCCGAGGCCCAGACCCGGGTCAGCCAGCTG
                  ************************************************************

HsCD00444478      GGCCTGCAAGTTGAGGGCCTGCGGCGGCGCCTGGAAGAGCTGCAGCAGGAGCTGAGCCTC
NM_144999.2       GGCCTGCAAGTTGAGGGCCTGCGGCGGCGCCTGGAAGAGCTGCAGCAGGAGCTGAGCCTC
                  ************************************************************

HsCD00444478      AAGGACCAGGAAAGGGTGGCCGAGGTGAGCAGGGTGCGCGTGGAGCTGCAGGAGCAGAAC
NM_144999.2       AAGGACCAGGAAAGGGTGGCCGAGGTGAGCAGGGTGCGCGTGGAGCTGCAGGAGCAGAAC
                  ************************************************************

HsCD00444478      GGCCGGCTGCAGGCGGAGCTGGCGGCTCAGGAGGCGCTGAGGGAGAAGGCGGCGGCCCTG
NM_144999.2       GGCCGGCTGCAGGCGGAGCTGGCGGCTCAGGAGGCGCTGAGGGAGAAGGCGGCGGCCCTG
                  ************************************************************

HsCD00444478      GAGCGCCAGCTGAAAGTGATGGCGAGCGACCACCGAGAGGCGCTGCTGGACAGGGAGAGC
NM_144999.2       GAGCGCCAGCTGAAAGTGATGGCGAGCGACCACCGAGAGGCGCTGCTGGACAGGGAGAGC
                  ************************************************************

HsCD00444478      GAGAACGCGTCTCTCCGGGAGAAGCTGCGGCTCCGGGAGGCGGAGATCGCCCGCATCCGG
NM_144999.2       GAGAACGCGTCTCTCCGGGAGAAGCTGCGGCTCCGGGAGGCGGAGATCGCCCGCATCCGG
                  ************************************************************

HsCD00444478      GACGAGGAGGCCCAGAGGGCGAGCTTCCTGCAGAACGCCGTCCTGGCTTACGTGCAGGCG
NM_144999.2       GACGAGGAGGCCCAGAGGGCGAGCTTCCTGCAGAACGCCGTCCTGGCTTACGTGCAGGCG
                  ************************************************************

HsCD00444478      TCCCCCGTGAGGACCCTGAGCCCCCCAAAG---
NM_144999.2       TCCCCCGTGAGGACCCTGAGCCCCCCAAAGTGA
                  ******************************   

Clone

Original Clone ID : ccsbBroad304_05197

Organism


TAX_ID : 9606
Species Specific ID: 201255

Genome

Chromosome : 17
Map Location : 17q25.3
Ensembl : ENSG00000169683

Reagents

Labome : LRRC45-antibody