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REQ (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  DPF2
Gene Name:  D4, zinc and double PHD fingers family 2
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH185960.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00044460

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1176nts         Open reading frame : 1 to 1176
Reference Sequence Alignment

ATGGCGGCTGTGGTGGAGAATGTAGTGAAGCTCCTTGGGGAGCAGTACTACAAAGATGCC
ATGGAGCAGTGCCACAATTACAATGCTCGCCTCTGTGCTGAGCGCAGCGTGCGCCTGCCT
TTCTTGGACTCACAGACCGGAGTAGCCCAGAGCAATTGTTACATCTGGATGGAAAAGCGA
CACCGGGGTCCAGGATTGGCCTCCGGACAGCTGTACTCCTACCCTGCCCGGCGCTGGCGG
AAAAAGCGGCGAGCCCATCCCCCTGAGGATCCACGACTTTCCTTCCCATCTATTAAGCCA
GACACAGACCAGACCCTGAAGAAGGAGGGGCTGATCTCTCAGGATGGCAGTAGTTTAGAG
GCTCTGTTGCGCACTGACCCCCTGGAGAAGCGAGGTGCCCCGGATCCCCGAGTTGATGAT
GACAGCCTGGGCGAGTTTCCTGTGACCAACAGTCGAGCGCGAAAGCGGATCCTAGAACCA
GATGACTTCCTGGATGACCTCGATGATGAAGACTATGAAGAAGATACTCCCAAGCGTCGG
GGAAAGGGGAAATCCAAGGGTAAGGGTGTGGGCAGTGCCCGTAAGAAGCTGGATGCTTCC
ATCCTGGAGGACCGGGATAAGCCCTATGCCTGTGACATTTGTGGAAAACGTTACAAGAAC
CGACCAGGCCTCAGTTACCACTATGCCCACTCCCACTTGGCTGAGGAGGAGGGCGAGGAC
AAGGAAGACTCTCAACCACCCACTCCTGTTTCCCAGAGGTCTGAGGAGCAGAAATCCAAA
AAGGGTCCTGATGGATTGGCCTTGCCCAACAACTACTGTGACTTCTGCCTGGGGGACTCA
AAGATTAACAAGAAGACGGGACAACCCGAGGAGCTGGTGTCCTGTTCTGACTGTGGCCGC
TCAGGGCATCCATCTTGCCTCCAATTTACCCCCGTGATGATGGCGGCAGTGAAGACATAC
CGCTGGCAGTGCATCGAGTGCAAATGTTGCAATATCTGCGGCACCTCCGAGAATGACGAC
CAGTTGCTCTTCTGTGATGACTGCGATCGTGGCTACCACATGTACTGTCTCACCCCGTCC
ATGTCTGAGCCCCCTGAAGGAAGTTGGAGCTGCCACCTGTGTCTGGACCTGTTGAAAGAG
AAAGCTTCCATCTACCAGAACCAGAACTCCTCTTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1199
Start on reference sequence 24
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : DPF2
Symbol Nomenclature : DPF2
SYNONYM : REQ; UBID4; ubi-d4
Designation : BAF45D|BRG1-associated factor 45D|apoptosis response zinc finger protein|protein requiem|requiem, apoptosis response zinc finger|zinc finger protein ubi-d4
Full Nomenclature : D4, zinc and double PHD fingers family 2
GENEID : 5977
HGNC : 9964
MIM : 601671
Vega : OTTHUMG00000165985
Target GenBank: BC014889

 

Reference Sequence Alignment

  

HsCD00044460        ATGGCGGCTGTGGTGGAGAATGTAGTGAAGCTCCTTGGGGAGCAGTACTACAAAGATGCC
XM_005274149.1      ATGGCGGCTGTGGTGGAGAATGTAGTGAAGCTCCTTGGGGAGCAGTACTACAAAGATGCC
NM_006268.4         ATGGCGGCTGTGGTGGAGAATGTAGTGAAGCTCCTTGGGGAGCAGTACTACAAAGATGCC
                    ************************************************************

HsCD00044460        ATGGAGCAGTGCCACAATTACAATGCTCGCCTCTGTGCTGAGCGCAGCGTGCGCCTGCCT
XM_005274149.1      ATGGAGCAGTGCCACAATTACAATGCTCGCCTCTGTGCTGAGCGCAGCGTGCGCCTGCCT
NM_006268.4         ATGGAGCAGTGCCACAATTACAATGCTCGCCTCTGTGCTGAGCGCAGCGTGCGCCTGCCT
                    ************************************************************

HsCD00044460        TTCTTGGACTCACAGACCGGAGTAGCCCAGAGCAATTGTTACATCTGGATGGAAAAGCGA
XM_005274149.1      TTCTTGGACTCACAGACCGGAGTAGCCCAGAGCAATTGTTACATCTGGATGGAAAAGCGA
NM_006268.4         TTCTTGGACTCACAGACCGGAGTAGCCCAGAGCAATTGTTACATCTGGATGGAAAAGCGA
                    ************************************************************

HsCD00044460        CACCGGGGTCCAGGATTGGCCTCCGGACAGCTGTACTCCTACCCTGCCCGGCGCTGGCGG
XM_005274149.1      CACCGGGGTCCAGGATTGGCCTCCGGACAGCTGTACTCCTACCCTGCCCGGCGCTGGCGG
NM_006268.4         CACCGGGGTCCAGGATTGGCCTCCGGACAGCTGTACTCCTACCCTGCCCGGCGCTGGCGG
                    ************************************************************

HsCD00044460        AAAAAGCGGCGAGCCCATCCCCCTGAGGATCCACGACTTTCCTTCCCATCTATTAAGCCA
XM_005274149.1      AAAAAGCGGCGAGCCCATCCCCCTGAGGATCCACGACTTTCCTTCCCATCTATTAAGCCA
NM_006268.4         AAAAAGCGGCGAGCCCATCCCCCTGAGGATCCACGACTTTCCTTCCCATCTATTAAGCCA
                    ************************************************************

HsCD00044460        GACACAGACCAGACCCTGAAGAAGGAGGGGCTGATCTCTCAGGATGGCAGTAGTTTAGAG
XM_005274149.1      GACACAGACCAGACCCTGAAGAAGGAGGGGCTGATCTCTCAGGATGGCAGTAGTTTAGAG
NM_006268.4         GACACAGACCAGACCCTGAAGAAGGAGGGGCTGATCTCTCAGGATGGCAGTAGTTTAGAG
                    ************************************************************

HsCD00044460        GCTCTGTTGCGCACTGACCCCCTGGAGAAGCGAGGTGCCCCGGATCCCCGAGTTGATGAT
XM_005274149.1      GCTCTGTTGCGCACTGACCCCCTGGAGAAGCGAGGTGCCCCGGATCCCCGAGTTGATGAT
NM_006268.4         GCTCTGTTGCGCACTGACCCCCTGGAGAAGCGAGGTGCCCCGGATCCCCGAGTTGATGAT
                    ************************************************************

HsCD00044460        GACAGCCTGGGCGAGTTTCCTGTGACCAACAGTCGAGCGCGAAAGCGGATCCTAGAACCA
XM_005274149.1      GACAGCCTGGGCGAGTTTCCTGTGACCAACAGTCGAGCGCGAAAGCGGATCCTAGAACCA
NM_006268.4         GACAGCCTGGGCGAGTTTCCTGTGACCAACAGTCGAGCGCGAAAGCGGATCCTAGAACCA
                    ************************************************************

HsCD00044460        GATGACTTCCTGGATGACCTCGATGATGAAGACTATGAAGAAGATACTCCCAAGCGTCGG
XM_005274149.1      GATGACTTCCTGGATGACCTCGATGATGAAGACTATGAAGAAGATACTCCCAAGCGTCGG
NM_006268.4         GATGACTTCCTGGATGACCTCGATGATGAAGACTATGAAGAAGATACTCCCAAGCGTCGG
                    ************************************************************

HsCD00044460        GGAAAGGGGAAATCCAAGGGTAAGGGTGTGGGCAGTGCCCGTAAGAAGCTGGATGCTTCC
XM_005274149.1      GGAAAGGGGAAATCCAAGGGTAAGGGTGTGGGCAGTGCCCGTAAGAAGCTGGATGCTTCC
NM_006268.4         GGAAAGGGGAAATCCAAGGGTAAGGGTGTGGGCAGTGCCCGTAAGAAGCTGGATGCTTCC
                    ************************************************************

HsCD00044460        ATCCTGGAGGACCGGGATAAGCCCTATGCCTGTGACATTTG-------------------
XM_005274149.1      ATCCTGGAGGACCGGGATAAGCCCTATGCCTGTGACAATAGTTTCAAACAAAAGCATACC
NM_006268.4         ATCCTGGAGGACCGGGATAAGCCCTATGCCTGTGACATTTG-------------------
                    *************************************:*:*                   

HsCD00044460        -----------------------TGGAAAACGTTACAAGAACCGACCAGGCCTCAGTTAC
XM_005274149.1      TCGAAAGCGCCCCAGAGAGTTTGTGGAAAACGTTACAAGAACCGACCAGGCCTCAGTTAC
NM_006268.4         -----------------------TGGAAAACGTTACAAGAACCGACCAGGCCTCAGTTAC
                                           *************************************

HsCD00044460        CACTATGCCCACTCCCACTTGGCTGAGGAGGAGGGCGAGGACAAGGAAGACTCTCAACCA
XM_005274149.1      CACTATGCCCACTCCCACTTGGCTGAGGAGGAGGGCGAGGACAAGGAAGACTCTCAACCA
NM_006268.4         CACTATGCCCACTCCCACTTGGCTGAGGAGGAGGGCGAGGACAAGGAAGACTCTCAACCA
                    ************************************************************

HsCD00044460        CCCACTCCTGTTTCCCAGAGGTCTGAGGAGCAGAAATCCAAAAAGGGTCCTGATGGATTG
XM_005274149.1      CCCACTCCTGTTTCCCAGAGGTCTGAGGAGCAGAAATCCAAAAAGGGTCCTGATGGATTG
NM_006268.4         CCCACTCCTGTTTCCCAGAGGTCTGAGGAGCAGAAATCCAAAAAGGGTCCTGATGGATTG
                    ************************************************************

HsCD00044460        GCCTTGCCCAACAACTACTGTGACTTCTGCCTGGGGGACTCAAAGATTAACAAGAAGACG
XM_005274149.1      GCCTTGCCCAACAACTACTGTGACTTCTGCCTGGGGGACTCAAAGATTAACAAGAAGACG
NM_006268.4         GCCTTGCCCAACAACTACTGTGACTTCTGCCTGGGGGACTCAAAGATTAACAAGAAGACG
                    ************************************************************

HsCD00044460        GGACAACCCGAGGAGCTGGTGTCCTGTTCTGACTGTGGCCGCTCAGGGCATCCATCTTGC
XM_005274149.1      GGACAACCCGAGGAGCTGGTGTCCTGTTCTGACTGTGGCCGCTCAGGGCATCCATCTTGC
NM_006268.4         GGACAACCCGAGGAGCTGGTGTCCTGTTCTGACTGTGGCCGCTCAGGGCATCCATCTTGC
                    ************************************************************

HsCD00044460        CTCCAATTTACCCCCGTGATGATGGCGGCAGTGAAGACATACCGCTGGCAGTGCATCGAG
XM_005274149.1      CTCCAATTTACCCCCGTGATGATGGCGGCAGTGAAGACATACCGCTGGCAGTGCATCGAG
NM_006268.4         CTCCAATTTACCCCCGTGATGATGGCGGCAGTGAAGACATACCGCTGGCAGTGCATCGAG
                    ************************************************************

HsCD00044460        TGCAAATGTTGCAATATCTGCGGCACCTCCGAGAATGACGACCAGTTGCTCTTCTGTGAT
XM_005274149.1      TGCAAATGTTGCAATATCTGCGGCACCTCCGAGAATGACGACCAGTTGCTCTTCTGTGAT
NM_006268.4         TGCAAATGTTGCAATATCTGCGGCACCTCCGAGAATGACGACCAGTTGCTCTTCTGTGAT
                    ************************************************************

HsCD00044460        GACTGCGATCGTGGCTACCACATGTACTGTCTCACCCCGTCCATGTCTGAGCCCCCTGAA
XM_005274149.1      GACTGCGATCGTGGCTACCACATGTACTGTCTCACCCCGTCCATGTCTGAGCCCCCTGAA
NM_006268.4         GACTGCGATCGTGGCTACCACATGTACTGTCTCACCCCGTCCATGTCTGAGCCCCCTGAA
                    ************************************************************

HsCD00044460        GGAAGTTGGAGCTGCCACCTGTGTCTGGACCTGTTGAAAGAGAAAGCTTCCATCTACCAG
XM_005274149.1      GGAAGTTGGAGCTGCCACCTGTGTCTGGACCTGTTGAAAGAGAAAGCTTCCATCTACCAG
NM_006268.4         GGAAGTTGGAGCTGCCACCTGTGTCTGGACCTGTTGAAAGAGAAAGCTTCCATCTACCAG
                    ************************************************************

HsCD00044460        AACCAGAACTCCTCTTAG
XM_005274149.1      AACCAGAACTCCTCTTGA
NM_006268.4         AACCAGAACTCCTCTTGA
                    ****************..

Protein

SMART domain : ZnF_C2H2 : zinc finger
SMART domain : PHD : PHD zinc finger
UniProt : Q92785
HPRD : 03394

Clone

Cloning Information : 185960
HIP Master Clone ID : 160904
Original Clone ID : FLH185960.01X

Organism


TAX_ID : 9606
Species Specific ID: 5977

Genome

Chromosome : 11
Map Location : 11q13
Ensembl : ENSG00000133884

Reagents

Labome : DPF2-antibody