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ZNFN1A5 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  IKZF5
Gene Name:  IKAROS family zinc finger 5 (Pegasus)
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH185998.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00044463

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1263nts         Open reading frame : 1 to 1263
Reference Sequence Alignment

ATGGGTGAAAAAAAACCAGAGCCTTTGGACTTCGTGAAAGATTTTCAGGAATACCTGACT
CAGCAGACCCATCACGTGAACATGATTTCTGGATCAGTTAGTGGGGACAAAGAAGCAGAG
GCTCTTCAGGGAGCTGGAACAGATGGTGATCAAAATGGACTTGATCACCCATCTGTTGAA
GTTTCCTTGGATGAAAACTCAGGAATGTTAGTAGACGGGTTTGAAAGGACCTTTGATGGG
AAGCTTAAGTGTCGGTACTGCAACTATGCCAGCAAAGGAACAGCCCGGCTTATTGAACAC
ATCAGAATCCACACAGGTGAAAAACCTCATCGATGTCATCTTTGTCCATTTGCATCTGCT
TATGAGCGTCATCTGGAAGCCCATATGCGTTCTCATACTGGAGAAAAACCATACAAATGT
GAATTATGTTCCTTCCGCTGCAGTGATCGAAGTAACTTGTCCCATCATCGAAGGCGCAAG
CATAAAATGGTACCAATTAAAGGTACTAGGTCTTCCTTAAGCAGCAAGAAAATGTGGGGG
GTTTTACAGAAGAAAACAAGCAATCTGGGCTATAGCAGAAGAGCACTAATCAACTTAAGT
CCACCTTCCGTGGTGGTTCAGAAACCAGACTACCTTAACGATTTTACCCACGAAATCCCA
AATATCCAGACTGACTCCTATGAAAGTATGGCAAAAACCACACCAACTGGTGGCCTTCCA
AGGGACCCCCAAGAACTCATGGTTGATAACCCTTTGAATCAGCTCTCGACTCTAGCAGGG
CAGTTGTCCAGTCTGCCACCCGAAAACCAAAACCCTGCATCACCTGATGTAGTTCCCTGC
CCTGATGAAAAGCCTTTCATGATTCAGCAGCCCTCTACCCAAGCAGTAGTTTCTGCCGTA
TCAGCAAGTATTCCTCAGAGCTCCTCTCCCACAAGCCCAGAACCTCGGCCATCCCATAGT
CAAAGGAACTATAGTCCAGTGGCAGGTCCAAGCAGTGAGCCAAGTGCCCACACGAGCACT
CCCAGCATAGGAAACAGCCAGCCAAGCACCCCAGCCCCAGCCCTGCCGGTCCAGGACCCT
CAGCTTCTGCACCACTGCCAGCACTGTGATATGTACTTTGCAGACAACATCCTTTACACT
ATTCATATGGGATGTCATGGGTATGAAAATCCTTTTCAGTGTAATATATGTGGATGCAAA
TGTAAAAACAAGTATGATTTTGCCTGTCATTTTGCAAGAGGGCACATAACCAACATTGAT
TAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1572
Start on reference sequence 310
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : IKZF5
Symbol Nomenclature : IKZF5
SYNONYM : PEGASUS; ZNFN1A5
Designation : zinc finger protein Pegasus|zinc finger protein, subfamily 1A, 5 (Pegasus)|zinc finger transcription factor Pegasus
Full Nomenclature : IKAROS family zinc finger 5 (Pegasus)
GENEID : 64376
HGNC : 14283
MIM : 606238
Vega : OTTHUMG00000019192
Target GenBank: BC022564

 

Reference Sequence Alignment

  

HsCD00044463        ATGGGTGAAAAAAAACCAGAGCCTTTGGACTTCGTGAAAGATTTTCAGGAATACCTGACT
NM_001271840.1      ATGGGTGAAAAAAAACCAGAGCCTTTGGACTTCGTGAAAGATTTTCAGGAATACCTGACT
                    ************************************************************

HsCD00044463        CAGCAGACCCATCACGTGAACATGATTTCTGGATCAGTTAGTGGGGACAAAGAAGCAGAG
NM_001271840.1      CAGCAGACCCATCACGTGAACATGATTTCTGGATCAGTTAGTGGGGACAAAGAAGCAGAG
                    ************************************************************

HsCD00044463        GCTCTTCAGGGAGCTGGAACAGATGGTGATCAAAATGGACTTGATCACCCATCTGTTGAA
NM_001271840.1      GCTCTTCAGGGAGCTGGAACAGATGGTGATCAAAATGGACTTGATCACCCATCTGTTGAA
                    ************************************************************

HsCD00044463        GTTTCCTTGGATGAAAACTCAGGAATGTTAGTAGACGGGTTTGAAAGGACCTTTGATGGG
NM_001271840.1      GTTTCCTTGGATGAAAACTCAGGAATGTTAGTAGACGGGTTTGAAAGGACCTTTGATGGG
                    ************************************************************

HsCD00044463        AAGCTTAAGTGTCGGTACTGCAACTATGCCAGCAAAGGAACAGCCCGGCTTATTGAACAC
NM_001271840.1      AAGCTTAAGTGTCGGTACTGCAACTATGCCAGCAAAGGAACAGCCCGGCTTATTGAACAC
                    ************************************************************

HsCD00044463        ATCAGAATCCACACAGGTGAAAAACCTCATCGATGTCATCTTTGTCCATTTGCATCTGCT
NM_001271840.1      ATCAGAATCCACACAGGTGAAAAACCTCATCGATGTCATCTTTGTCCATTTGCATCTGCT
                    ************************************************************

HsCD00044463        TATGAGCGTCATCTGGAAGCCCATATGCGTTCTCATACTGGAGAAAAACCATACAAATGT
NM_001271840.1      TATGAGCGTCATCTGGAAGCCCATATGCGTTCTCATACTGGAGAAAAACCATACAAATGT
                    ************************************************************

HsCD00044463        GAATTATGTTCCTTCCGCTGCAGTGATCGAAGTAACTTGTCCCATCATCGAAGGCGCAAG
NM_001271840.1      GAATTATGTTCCTTCCGCTGCAGTGATCGAAGTAACTTGTCCCATCATCGAAGGCGCAAG
                    ************************************************************

HsCD00044463        CATAAAATGGTACCAATTAAAGGTACTAGGTCTTCCTTAAGCAGCAAGAAAATGTGGGGG
NM_001271840.1      CATAAAATGGTACCAATTAAAGGTACTAGGTCTTCCTTAAGCAGCAAGAAAATGTGGGGG
                    ************************************************************

HsCD00044463        GTTTTACAGAAGAAAACAAGCAATCTGGGCTATAGCAGAAGAGCACTAATCAACTTAAGT
NM_001271840.1      GTTTTACAGAAGAAAACAAGCAATCTGGGCTATAGCAGAAGAGCACTAATCAACTTAAGT
                    ************************************************************

HsCD00044463        CCACCTTCCGTGGTGGTTCAGAAACCAGACTACCTTAACGATTTTACCCACGAAATCCCA
NM_001271840.1      CCACCTTCCATGGTGGTTCAGAAACCAGACTACCTTAACGATTTTACCCACGAAATCCCA
                    *********.**************************************************

HsCD00044463        AATATCCAGACTGACTCCTATGAAAGTATGGCAAAAACCACACCAACTGGTGGCCTTCCA
NM_001271840.1      AATATCCAGACTGACTCCTATGAAAGTATGGCAAAAACCACACCAACTGGTGGCCTTCCA
                    ************************************************************

HsCD00044463        AGGGACCCCCAAGAACTCATGGTTGATAACCCTTTGAATCAGCTCTCGACTCTAGCAGGG
NM_001271840.1      AGGGACCCCCAAGAACTCATGGTTGATAACCCTTTGAATCAGCTCTCGACTCTAGCAGGG
                    ************************************************************

HsCD00044463        CAGTTGTCCAGTCTGCCACCCGAAAACCAAAACCCTGCATCACCTGATGTAGTTCCCTGC
NM_001271840.1      CAGTTGTCCAGTCTGCCACCCGAAAACCAAAACCCTGCATCCCCTGATGTAGTTCCCTGC
                    *****************************************.******************

HsCD00044463        CCTGATGAAAAGCCTTTCATGATTCAGCAGCCCTCTACCCAAGCAGTAGTTTCTGCCGTA
NM_001271840.1      CCTGATGAAAAGCCTTTCATGATTCAGCAGCCCTCTACCCAAGCAGTAGTTTCTGCCGTA
                    ************************************************************

HsCD00044463        TCAGCAAGTATTCCTCAGAGCTCCTCTCCCACAAGCCCAGAACCTCGGCCATCCCATAGT
NM_001271840.1      TCAGCAAGTATTCCTCAGAGCTCCTCTCCCACAAGCCCAGAACCTCGGCCATCCCATAGT
                    ************************************************************

HsCD00044463        CAAAGGAACTATAGTCCAGTGGCAGGTCCAAGCAGTGAGCCAAGTGCCCACACGAGCACT
NM_001271840.1      CAAAGGAACTATAGTCCAGTGGCAGGTCCAAGCAGTGAGCCAAGTGCCCACACGAGCACT
                    ************************************************************

HsCD00044463        CCCAGCATAGGAAACAGCCAGCCAAGCACCCCAGCCCCAGCCCTGCCGGTCCAGGACCCT
NM_001271840.1      CCCAGCATAGGAAACAGCCAGCCAAGCACCCCAGCCCCAGCCCTGCCGGTCCAGGACCCT
                    ************************************************************

HsCD00044463        CAGCTTCTGCACCACTGCCAGCACTGTGATATGTACTTTGCAGACAACATCCTTTACACT
NM_001271840.1      CAGCTTCTGCACCACTGCCAGCACTGTGATATGTACTTTGCAGACAACATCCTTTACACT
                    ************************************************************

HsCD00044463        ATTCATATGGGATGTCATGGGTATGAAAATCCTTTTCAGTGTAATATATGTGGATGCAAA
NM_001271840.1      ATTCATATGGGATGTCATGGGTATGAAAATCCTTTTCAGTGTAATATATGTGGATGCAAA
                    ************************************************************

HsCD00044463        TGTAAAAACAAGTATGATTTTGCCTGTCATTTTGCAAGAGGGCACATAACCAACATTGAT
NM_001271840.1      TGTAAAAACAAGTATGATTTTGCCTGTCATTTTGCAAGAGGGCAACATAACCAACATTGA
                    ********************************************..::*.*.*..:* .:

HsCD00044463        TAG
NM_001271840.1      ---
                       

Protein

SMART domain : ZnF_C2H2 : zinc finger
UniProt : Q9H5V7
HPRD : 05873

Clone

Cloning Information : 185998
HIP Master Clone ID : 160942
Original Clone ID : FLH185998.01X

Organism


TAX_ID : 9606
Species Specific ID: 64376

Genome

Chromosome : 10
Map Location : 10q26
Ensembl : ENSG00000095574

Reagents

Labome : IKZF5-antibody