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UBE2R2 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  UBE2R2
Gene Name:  ubiquitin-conjugating enzyme E2R 2
Sequence             
Original Clone ID: ccsbBroad304_03483
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00444706

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 715nts         Open reading frame : 1 to 715
Reference Sequence Alignment

ATGGCCCAGCAGCAGATGACCAGCTCGCAGAAGGCCCTGATGCTCGAGCTGAAATCCCTG
CAGGAGGAACCGGTGGAGGGCTTCCGGATCACCCTGGTGGACGAGTCCGACCTCTACAAC
TGGGAGGTGGCCATCTTCGGACCCCCCAACACCCTCTACGAAGGCGGCTACTTCAAGGCG
CATATTAAATTTCCTATTGACTACCCCTATTCACCACCTACCTTCAGATTCTTGACCAAA
ATGTGGCACCCCAACATTTATGAGAATGGAGATGTATGCATTTCGATTCTTCATCCGCCT
GTAGATGACCCACAGAGTGGAGAACTGCCTTCTGAAAGGTGGAATCCTACTCAGAATGTG
AGGACTATCCTATTAAGTGTAATCTCACTGCTTAATGAGCCCAACACCTTCTCCCCAGCC
AATGTCGATGCTTCAGTTATGTTCAGGAAATGGAGAGACAGTAAAGGAAAAGACAAAGAA
TATGCTGAAATTATTAGGAAACAAGTTTCAGCCACTAAGGCCGAAGCAGAAAAGGATGGA
GTGAAGGTCCCCACAACCCTGGCGGAATACTGCATCAAAACTAAAGTGCCTTCCAATGAC
AACAGCTCAGATTTGCTTTACGACGACTTGTATGATGACGACATTGATGATGAAGATGAG
GAGGAGGAAGATGCCGACTGTTATGATGATGATGATTCTGGGAATGAGGAGTCG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : UBE2R2
Symbol Nomenclature : UBE2R2
SYNONYM : CDC34B; E2-CDC34B; UBC3B
Designation : ubiquitin carrier protein R2|ubiquitin-conjugating enzyme E2 R2|ubiquitin-conjugating enzyme E2-CDC34B|ubiquitin-conjugating enzyme UBC3B|ubiquitin-protein ligase R2
Full Nomenclature : ubiquitin-conjugating enzyme E2R 2
GENEID : 54926
Locus Tag : RP11-176F3.4
HGNC : 19907
MIM : 612506
Vega : OTTHUMG00000019797
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00444706      ATGGCCCAGCAGCAGATGACCAGCTCGCAGAAGGCCCTGATGCTCGAGCTGAAATCCCTG
NM_017811.3       ATGGCCCAGCAGCAGATGACCAGCTCGCAGAAGGCCCTGATGCTCGAGCTGAAATCCCTG
                  ************************************************************

HsCD00444706      CAGGAGGAACCGGTGGAGGGCTTCCGGATCACCCTGGTGGACGAGTCCGACCTCTACAAC
NM_017811.3       CAGGAGGAACCGGTGGAGGGCTTCCGGATCACCCTGGTGGACGAGTCCGACCTCTACAAC
                  ************************************************************

HsCD00444706      TGGGAGGTGGCCATCTTCGGACCCCCCAACACCCTCTACGAAGGCGGCTACTTCAAGGCG
NM_017811.3       TGGGAGGTGGCCATCTTCGGACCCCCCAACACCCTCTACGAAGGCGGCTACTTCAAGGCG
                  ************************************************************

HsCD00444706      CATATTAAATTTCCTATTGACTACCCCTATTCACCACCTACCTTCAGATTCTTGACCAAA
NM_017811.3       CATATTAAATTTCCTATTGACTACCCCTATTCACCACCTACCTTCAGATTCTTGACCAAA
                  ************************************************************

HsCD00444706      ATGTGGCACCCCAACATTTATGAGAATGGAGATGTATGCATTTCGATTCTTCATCCGCCT
NM_017811.3       ATGTGGCACCCCAACATTTATGAGAATGGAGATGTATGCATTTCGATTCTTCATCCGCCT
                  ************************************************************

HsCD00444706      GTAGATGACCCACAGAGTGGAGAACTGCCTTCTGAAAGGTGGAATCCTACTCAGAATGTG
NM_017811.3       GTAGATGACCCACAGAGTGGAGAACTGCCTTCTGAAAGGTGGAATCCTACTCAGAATGTG
                  ************************************************************

HsCD00444706      AGGACTATCCTATTAAGTGTAATCTCACTGCTTAATGAGCCCAACACCTTCTCCCCAGCC
NM_017811.3       AGGACTATCCTATTAAGTGTAATCTCACTGCTTAATGAGCCCAACACCTTCTCCCCAGCC
                  ************************************************************

HsCD00444706      AATGTCGATGCTTCAGTTATGTTCAGGAAATGGAGAGACAGTAAAGGAAAAGACAAAGAA
NM_017811.3       AATGTCGATGCTTCAGTTATGTTCAGGAAATGGAGAGACAGTAAAGGAAAAGACAAAGAA
                  ************************************************************

HsCD00444706      TATGCTGAAATTATTAGGAAACAAGTTTCAGCCACTAAGGCCGAAGCAGAAAAGGATGGA
NM_017811.3       TATGCTGAAATTATTAGGAAACAAGTTTCAGCCACTAAGGCCGAAGCAGAAAAGGATGGA
                  ************************************************************

HsCD00444706      GTGAAGGTCCCCACAACCCTGGCGGAATACTGCATCAAAACTAAAGTGCCTTCCAATGAC
NM_017811.3       GTGAAGGTCCCCACAACCCTGGCGGAATACTGCATCAAAACTAAAGTGCCTTCCAATGAC
                  ************************************************************

HsCD00444706      AACAGCTCAGATTTGCTTTACGACGACTTGTATGATGACGACATTGATGATGAAGATGAG
NM_017811.3       AACAGCTCAGATTTGCTTTACGACGACTTGTATGATGACGACATTGATGATGAAGATGAG
                  ************************************************************

HsCD00444706      GAGGAGGAAGATGCCGACTGTTATGATGATGATGATTCTGGGAATGAGGAGTCG---
NM_017811.3       GAGGAGGAAGATGCCGACTGTTATGATGATGATGATTCTGGGAATGAGGAGTCGTGA
                  ******************************************************   

Pathways

Reactome : Adaptive Immune System
Reactome : Antigen processing: Ubiquitination & Proteasome degradation
Reactome : Class I MHC mediated antigen processing & presentation
Reactome : Immune System

Clone

Original Clone ID : ccsbBroad304_03483

Organism


TAX_ID : 9606
Species Specific ID: 54926

Genome

Chromosome : 9
Map Location : 9p13.3
Ensembl : ENSG00000107341

Reagents

Labome : UBC3B-antibody