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PPP6C (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  PPP6C
Gene Name:  protein phosphatase 6, catalytic subunit
Sequence             
Original Clone ID: ccsbBroad304_01270
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00444800

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 916nts         Open reading frame : 1 to 916
Reference Sequence Alignment

ATGGCGCCGCTAGACCTGGACAAGTATGTGGAAATAGCGCGGCTGTGCAAGTACCTGCCA
GAGAACGACCTGAAGCGGCTATGTGACTACGTTTGTGACCTCCTCTTAGAAGAGTCAAAT
GTTCAGCCAGTATCAACACCAGTAACAGTGTGTGGAGATATCCATGGACAGTTTTATGAC
CTTTGTGAACTGTTCAGAACTGGAGGTCAGGTTCCTGACACAAACTACATATTTATGGGT
GATTTTGTAGACAGAGGTTACTATAGTTTGGAGACCTTCACTTACCTTCTTGCATTAAAG
GCTAAATGGCCTGATCGTATTACACTTTTGCGAGGAAATCATGAGAGTAGACAGATAACA
CAGGTCTATGGATTTTATGATGAGTGCCAAACCAAATATGGAAATGCTAATGCCTGGAGA
TACTGTACCAAAGTTTTTGACATGCTCACAGTAGCAGCTTTAATAGATGAGCAGATTTTG
TGTGTCCATGGTGGTTTATCTCCTGATATCAAAACACTGGATCAAATTCGAACCATCGAA
CGGAATCAGGAAATTCCTCATAAAGGAGCATTTTGTGATCTGGTTTGGTCAGATCCTGAA
GATGTGGATACCTGGGCTATCAGTCCCCGAGGAGCAGGTTGGCTTTTTGGAGCAAAGGTC
ACAAATGAGTTTGTTCATATCAACAACTTAAAACTCATCTGCAGAGCACATCAACTAGTG
CACGAAGGCTATAAATTTATGTTTGATGAGAAGCTGGTGACAGTATGGTCTGCTCCTAAT
TACTGCTATCGTTGTGGAAATATTGCTTCGATCATGGTCTTCAAAGATGTAAATACAAGA
GAACCAAAGTTATTCCGGGCAGTTCCAGATTCAGAACGTGTTATTCCTCCCAGAACGACA
ACGCCATATTTCCTT


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : PPP6C
Symbol Nomenclature : PPP6C
SYNONYM : PP6; PP6C
Designation : serine/threonine protein phosphatase catalytic subunit|serine/threonine-protein phosphatase 6 catalytic subunit
Full Nomenclature : protein phosphatase 6, catalytic subunit
GENEID : 5537
Locus Tag : RP11-366O20.1
HGNC : 9323
MIM : 612725
Vega : OTTHUMG00000020671
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00444800        ATGGCGCCGCTAGACCTGGACAAGTATGTGGAAATAGCGCGGCTGTGCAAGTACCTGCCA
NM_002721.4         ATGGCGCCGCTAGACCTGGACAAGTATGTGGAAATAGCGCGGCTGTGCAAGTACCTGCCA
NM_001123369.1      ATGGCGCCGCTAGACCTGGACAAGTATGTGGAAATAGCGCGGCTGTGCAAGTACCTGCCA
                    ************************************************************

HsCD00444800        GAGAACGACCTGAAGCGGCTATGTGACTACGTTTGTGACCTCCTCTTAGAAGAGTCAAAT
NM_002721.4         GAGAACGACCTGAAGCGGCTATGTGACTACGTTTGTGACCTCCTCTTAGAAGAGTCAAAT
NM_001123369.1      GAGAACGACCTGAAGCGGCTATGTGACTACGTTTGTGACCTCCTCTTAGAAGAGTCAAAT
                    ************************************************************

HsCD00444800        GTTCAGCCAGTATCAACACCAGTAACAGTGTGTGGAGATATCCATGGACAGTTTTATGAC
NM_002721.4         GTTCAGCCAGTATCAACACCAGTAACAGTGTGTGGAGATATCCATGGACAGTTTTATGAC
NM_001123369.1      GTTCAGCCAGTATCAACACCAGTAACAGTGTGTGGAGATATCCATG--------------
                    **********************************************              

HsCD00444800        CTTTGTGAACTGTTCAGAACTGGAGGTCAGGTTCCTGACACAAACTACATATTTATGGGT
NM_002721.4         CTTTGTGAACTGTTCAGAACTGGAGGTCAGGTTCCTGACACAAACTACATATTTATGGGT
NM_001123369.1      ----------------------------------------------------GACAGGGT
                                                                         :.:****

HsCD00444800        GATTTTGTAGACAGAGGTTACTATAGTTTGGAGACCTTCACTTACCTTCTTGCATTAAAG
NM_002721.4         GATTTTGTAGACAGAGGTTACTATAGTTTGGAGACCTTCACTTACCTTCTTGCATTAAAG
NM_001123369.1      GATTTTGTAGACAGAGGTTACTATAGTTTGGAGACCTTCACTTACCTTCTTGCATTAAAG
                    ************************************************************

HsCD00444800        GCTAAATGGCCTGATCGTATTACACTTTTGCGAGGAAATCATGAGAGTAGACAGATAACA
NM_002721.4         GCTAAATGGCCTGATCGTATTACACTTTTGCGAGGAAATCATGAGAGTAGACAGATAACA
NM_001123369.1      GCTAAATGGCCTGATCGTATTACACTTTTGCGAGGAAATCATGAGAGTAGACAGATAACA
                    ************************************************************

HsCD00444800        CAGGTCTATGGATTTTATGATGAGTGCCAAACCAAATATGGAAATGCTAATGCCTGGAGA
NM_002721.4         CAGGTCTATGGATTTTATGATGAGTGCCAAACCAAATATGGAAATGCTAATGCCTGGAGA
NM_001123369.1      CAGGTCTATGGATTTTATGATGAGTGCCAAACCAAATATGGAAATGCTAATGCCTGGAGA
                    ************************************************************

HsCD00444800        TACTGTACCAAAGTTTTTGACATGCTCACAGTAGCAGCTTTAATAGATGAGCAGATTTTG
NM_002721.4         TACTGTACCAAAGTTTTTGACATGCTCACAGTAGCAGCTTTAATAGATGAGCAGATTTTG
NM_001123369.1      TACTGTACCAAAGTTTTTGACATGCTCACAGTAGCAGCTTTAATAGATGAGCAGATTTTG
                    ************************************************************

HsCD00444800        TGTGTCCATGGTGGTTTATCTCCTGATATCAAAACACTGGATCAAATTCGAACCATCGAA
NM_002721.4         TGTGTCCATGGTGGTTTATCTCCTGATATCAAAACACTGGATCAAATTCGAACCATCGAA
NM_001123369.1      TGTGTCCATGGTGGTTTATCTCCTGATATCAAAACACTGGATCAAATTCGAACCATCGAA
                    ************************************************************

HsCD00444800        CGGAATCAGGAAATTCCTCATAAAGGAGCATTTTGTGATCTGGTTTGGTCAGATCCTGAA
NM_002721.4         CGGAATCAGGAAATTCCTCATAAAGGAGCATTTTGTGATCTGGTTTGGTCAGATCCTGAA
NM_001123369.1      CGGAATCAGGAAATTCCTCATAAAGGAGCATTTTGTGATCTGGTTTGGTCAGATCCTGAA
                    ************************************************************

HsCD00444800        GATGTGGATACCTGGGCTATCAGTCCCCGAGGAGCAGGTTGGCTTTTTGGAGCAAAGGTC
NM_002721.4         GATGTGGATACCTGGGCTATCAGTCCCCGAGGAGCAGGTTGGCTTTTTGGAGCAAAGGTC
NM_001123369.1      GATGTGGATACCTGGGCTATCAGTCCCCGAGGAGCAGGTTGGCTTTTTGGAGCAAAGGTC
                    ************************************************************

HsCD00444800        ACAAATGAGTTTGTTCATATCAACAACTTAAAACTCATCTGCAGAGCACATCAACTAGTG
NM_002721.4         ACAAATGAGTTTGTTCATATCAACAACTTAAAACTCATCTGCAGAGCACATCAACTAGTG
NM_001123369.1      ACAAATGAGTTTGTTCATATCAACAACTTAAAACTCATCTGCAGAGCACATCAACTAGTG
                    ************************************************************

HsCD00444800        CACGAAGGCTATAAATTTATGTTTGATGAGAAGCTGGTGACAGTATGGTCTGCTCCTAAT
NM_002721.4         CACGAAGGCTATAAATTTATGTTTGATGAGAAGCTGGTGACAGTATGGTCTGCTCCTAAT
NM_001123369.1      CACGAAGGCTATAAATTTATGTTTGATGAGAAGCTGGTGACAGTATGGTCTGCTCCTAAT
                    ************************************************************

HsCD00444800        TACTGCTATCGTTGTGGAAATATTGCTTCGATCATGGTCTTCAAAGATGTAAATACAAGA
NM_002721.4         TACTGCTATCGTTGTGGAAATATTGCTTCGATCATGGTCTTCAAAGATGTAAATACAAGA
NM_001123369.1      TACTGCTATCGTTGTGGAAATATTGCTTCGATCATGGTCTTCAAAGATGTAAATACAAGA
                    ************************************************************

HsCD00444800        GAACCAAAGTTATTCCGGGCAGTTCCAGATTCAGAACGTGTTATTCCTCCCAGAACGACA
NM_002721.4         GAACCAAAGTTATTCCGGGCAGTTCCAGATTCAGAACGTGTTATTCCTCCCAGAACGACA
NM_001123369.1      GAACCAAAGTTATTCCGGGCAGTTCCAGATTCAGAACGTGTTATTCCTCCCAGAACGACA
                    ************************************************************

HsCD00444800        ACGCCATATTTCCTT---
NM_002721.4         ACGCCATATTTCCTTTGA
NM_001123369.1      ACGCCATATTTCCTTTGA
                    ***************   

Clone

Original Clone ID : ccsbBroad304_01270

Organism


TAX_ID : 9606
Species Specific ID: 5537

Genome

Chromosome : 9
Map Location : 9q33.3
Ensembl : ENSG00000119414

Reagents

Labome : PPP6C-antibody
Labome : PPP6C-siRNA