DNASU Plasmid Repository • 480.965.5697 | Email

HSPA6 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  HSPA6
Gene Name:  heat shock 70kDa protein 6 (HSP70B')
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH186393.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 24556684
Title: Dysfunction of the stress-responsive FOXC1 transcription factor contributes to the earlier-onset glaucoma observed in Axenfeld-Rieger syndrome patients
Authors: HIP
HsCD00044509

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1932nts         Open reading frame : 1 to 1932
Reference Sequence Alignment

ATGCAGGCCCCACGGGAGCTCGCGGTGGGCATCGACCTGGGCACCACCTACTCGTGCGTG
GGCGTGTTTCAGCAGGGCCGCGTGGAGATCCTGGCCAACGACCAGGGCAACCGCACCACG
CCCAGCTACGTGGCCTTCACCGACACCGAGCGGCTGGTCGGGGACGCGGCCAAGAGCCAG
GCGGCCCTGAACCCCCACAACACCGTGTTCGATGCCAAGCGGCTGATCGGGCGCAAGTTC
GCGGACACCACGGTGCAGTCGGACATGAAGCACTGGCCCTTCCGGGTGGTGAGCGAGGGC
GGCAAGCCCAAGGTGCGCGTATGCTACCGCGGGGAGGACAAGACGTTCTACCCCGAGGAG
ATCTCGTCCATGGTGCTGAGCAAGATGAAGGAGACGGCCGAGGCGTACCTGGGCCAGCCC
GTGAAGCACGCAGTGATCACCGTGCCCGCCTATTTCAATGACTCGCAGCGCCAGGCCACC
AAGGACGCGGGGGCCATCGCGGGGCTCAACGTGTTGCGGATCATCAATGAGCCCACGGCA
GCTGCCATCGCCTATGGGCTGGACCGGCGGGGCGCGGGAGAGCGCAACGTGCTCATTTTT
GACCTGGGTGGGGGCACCTTCGATGTGTCGGTTCTCTCCATTGACGCTGGTGTCTTTGAG
GTGAAAGCCACTGCTGGAGATACCCACCTGGGAGGAGAGGACTTCGACAACCGGCTCGTG
AACCACTTCATGGAAGAATTCCGGCGGAAGCATGGGAAGGACCTGAGCGGGAACAAGCGT
GCCCTGCGCAGGCTGCGCACAGCCTGTGAGCGCGCCAAGCGCACCCTGTCCTCCAGCACC
CAGGCCACCCTGGAGATAGACTCCCTGTTCGAGGGCGTGGACTTCTACACGTCCATCACT
CGTGCCCGCTTTGAGGAACTGTGCTCAGACCTCTTCCGCAGCACCCTGGAGCCGGTGGAG
AAGGCCCTGCGGGATGCCAAGCTGGACAAGGCCCAGATTCATGACGTCGTCCTGGTGGGG
GGCTCCACTCGCATCCCCAAGGTGCAGAAGTTGCTGCAGGACTTCTTCAACGGCAAGGAG
CTGAACAAGAGCATCAACCCTGATGAGGCTGTGGCCTATGGGGCTGCTGTGCAGGCGGCC
GTGTTGATGGGGGACAAATGTGAGAAAGTGCAGGATCTCCTGCTGCTGGATGTGGCTCCC
CTGTCTCTGGGGCTGGAGACAGCAGGTGGGGTGATGACCACGCTGATCCAGAGGAACGCC
ACTATCCCCACCAAGCAGACCCAGACTTTCACCACCTACTCGGACAACCAGCCTGGGGTC
TTCATCCAGGTGTATGAGGGTGAGAGGGCCATGACCAAGGACAACAACCTGCTGGGGCGT
TTTGAACTCAGTGGCATCCCTCCTGCCCCACGTGGAGTCCCCCAGATAGAGGTGACCTTT
GACATTGATGCTAATGGCATCCTGAGCGTGACAGCCACTGACAGGAGCACAGGTAAGGCT
AACAAGATCACCATCACCAATGACAAGGGCCGGCTGAGCAAGGAGGAGGTGGAGAGGATG
GTTCATGAAGCCGAGCAGTACAAGGCTGAGGATGAGGCCCAGAGGGACAGAGTGGCTGCC
AAAAACTCGCTGGAGGCCCATGTCTTCCATGTGAAAGGTTCTTTGCAAGAGGAAAGCCTT
AGGGACAAGATTCCCGAAGAGGACAGGCGCAAAATGCAAGACAAGTGTCGGGAAGTCCTT
GCCTGGCTGGAGCACAACCAGCTGGCAGAGAAGGAGGAGTATGAGCATCAGAAGAGGGAG
CTGGAGCAAATCTGTCGCCCCATCTTCTCCAGGCTCTATGGGGGGCCTGGTGTCCCTGGG
GGCAGCAGTTGTGGCACTCAAGCCCGCCAGGGGGACCCCAGCACCGGCCCCATCATTGAG
GAGGTTGATTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 2041
Start on reference sequence 110
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : HSPA6
Symbol Nomenclature : HSPA6
Designation : heat shock 70 kDa protein 6|heat shock 70 kDa protein B'|heat shock 70kD protein 6 (HSP70B')
Full Nomenclature : heat shock 70kDa protein 6 (HSP70B')
GENEID : 3310
HGNC : 5239
MIM : 140555
Vega : OTTHUMG00000034461
Target GenBank: BC035665

 

Reference Sequence Alignment

  

HsCD00044509      ATGCAGGCCCCACGGGAGCTCGCGGTGGGCATCGACCTGGGCACCACCTACTCGTGCGTG
NM_002155.3       ATGCAGGCCCCACGGGAGCTCGCGGTGGGCATCGACCTGGGCACCACCTACTCGTGCGTG
                  ************************************************************

HsCD00044509      GGCGTGTTTCAGCAGGGCCGCGTGGAGATCCTGGCCAACGACCAGGGCAACCGCACCACG
NM_002155.3       GGCGTGTTTCAGCAGGGCCGCGTGGAGATCCTGGCCAACGACCAGGGCAACCGCACCACG
                  ************************************************************

HsCD00044509      CCCAGCTACGTGGCCTTCACCGACACCGAGCGGCTGGTCGGGGACGCGGCCAAGAGCCAG
NM_002155.3       CCCAGCTACGTGGCCTTCACCGACACCGAGCGGCTGGTCGGGGACGCGGCCAAGAGCCAG
                  ************************************************************

HsCD00044509      GCGGCCCTGAACCCCCACAACACCGTGTTCGATGCCAAGCGGCTGATCGGGCGCAAGTTC
NM_002155.3       GCGGCCCTGAACCCCCACAACACCGTGTTCGATGCCAAGCGGCTGATCGGGCGCAAGTTC
                  ************************************************************

HsCD00044509      GCGGACACCACGGTGCAGTCGGACATGAAGCACTGGCCCTTCCGGGTGGTGAGCGAGGGC
NM_002155.3       GCGGACACCACGGTGCAGTCGGACATGAAGCACTGGCCCTTCCGGGTGGTGAGCGAGGGC
                  ************************************************************

HsCD00044509      GGCAAGCCCAAGGTGCGCGTATGCTACCGCGGGGAGGACAAGACGTTCTACCCCGAGGAG
NM_002155.3       GGCAAGCCCAAGGTGCGCGTATGCTACCGCGGGGAGGACAAGACGTTCTACCCCGAGGAG
                  ************************************************************

HsCD00044509      ATCTCGTCCATGGTGCTGAGCAAGATGAAGGAGACGGCCGAGGCGTACCTGGGCCAGCCC
NM_002155.3       ATCTCGTCCATGGTGCTGAGCAAGATGAAGGAGACGGCCGAGGCGTACCTGGGCCAGCCC
                  ************************************************************

HsCD00044509      GTGAAGCACGCAGTGATCACCGTGCCCGCCTATTTCAATGACTCGCAGCGCCAGGCCACC
NM_002155.3       GTGAAGCACGCAGTGATCACCGTGCCCGCCTATTTCAATGACTCGCAGCGCCAGGCCACC
                  ************************************************************

HsCD00044509      AAGGACGCGGGGGCCATCGCGGGGCTCAACGTGTTGCGGATCATCAATGAGCCCACGGCA
NM_002155.3       AAGGACGCGGGGGCCATCGCGGGGCTCAACGTGTTGCGGATCATCAATGAGCCCACGGCA
                  ************************************************************

HsCD00044509      GCTGCCATCGCCTATGGGCTGGACCGGCGGGGCGCGGGAGAGCGCAACGTGCTCATTTTT
NM_002155.3       GCTGCCATCGCCTATGGGCTGGACCGGCGGGGCGCGGGAGAGCGCAACGTGCTCATTTTT
                  ************************************************************

HsCD00044509      GACCTGGGTGGGGGCACCTTCGATGTGTCGGTTCTCTCCATTGACGCTGGTGTCTTTGAG
NM_002155.3       GACCTGGGTGGGGGCACCTTCGATGTGTCGGTTCTCTCCATTGACGCTGGTGTCTTTGAG
                  ************************************************************

HsCD00044509      GTGAAAGCCACTGCTGGAGATACCCACCTGGGAGGAGAGGACTTCGACAACCGGCTCGTG
NM_002155.3       GTGAAAGCCACTGCTGGAGATACCCACCTGGGAGGAGAGGACTTCGACAACCGGCTCGTG
                  ************************************************************

HsCD00044509      AACCACTTCATGGAAGAATTCCGGCGGAAGCATGGGAAGGACCTGAGCGGGAACAAGCGT
NM_002155.3       AACCACTTCATGGAAGAATTCCGGCGGAAGCATGGGAAGGACCTGAGCGGGAACAAGCGT
                  ************************************************************

HsCD00044509      GCCCTGCGCAGGCTGCGCACAGCCTGTGAGCGCGCCAAGCGCACCCTGTCCTCCAGCACC
NM_002155.3       GCCCTGCGCAGGCTGCGCACAGCCTGTGAGCGCGCCAAGCGCACCCTGTCCTCCAGCACC
                  ************************************************************

HsCD00044509      CAGGCCACCCTGGAGATAGACTCCCTGTTCGAGGGCGTGGACTTCTACACGTCCATCACT
NM_002155.3       CAGGCCACCCTGGAGATAGACTCCCTGTTCGAGGGCGTGGACTTCTACACGTCCATCACT
                  ************************************************************

HsCD00044509      CGTGCCCGCTTTGAGGAACTGTGCTCAGACCTCTTCCGCAGCACCCTGGAGCCGGTGGAG
NM_002155.3       CGTGCCCGCTTTGAGGAACTGTGCTCAGACCTCTTCCGCAGCACCCTGGAGCCGGTGGAG
                  ************************************************************

HsCD00044509      AAGGCCCTGCGGGATGCCAAGCTGGACAAGGCCCAGATTCATGACGTCGTCCTGGTGGGG
NM_002155.3       AAGGCCCTGCGGGATGCCAAGCTGGACAAGGCCCAGATTCATGACGTCGTCCTGGTGGGG
                  ************************************************************

HsCD00044509      GGCTCCACTCGCATCCCCAAGGTGCAGAAGTTGCTGCAGGACTTCTTCAACGGCAAGGAG
NM_002155.3       GGCTCCACTCGCATCCCCAAGGTGCAGAAGTTGCTGCAGGACTTCTTCAACGGCAAGGAG
                  ************************************************************

HsCD00044509      CTGAACAAGAGCATCAACCCTGATGAGGCTGTGGCCTATGGGGCTGCTGTGCAGGCGGCC
NM_002155.3       CTGAACAAGAGCATCAACCCTGATGAGGCTGTGGCCTATGGGGCTGCTGTGCAGGCGGCC
                  ************************************************************

HsCD00044509      GTGTTGATGGGGGACAAATGTGAGAAAGTGCAGGATCTCCTGCTGCTGGATGTGGCTCCC
NM_002155.3       GTGTTGATGGGGGACAAATGTGAGAAAGTGCAGGATCTCCTGCTGCTGGATGTGGCTCCC
                  ************************************************************

HsCD00044509      CTGTCTCTGGGGCTGGAGACAGCAGGTGGGGTGATGACCACGCTGATCCAGAGGAACGCC
NM_002155.3       CTGTCTCTGGGGCTGGAGACAGCAGGTGGGGTGATGACCACGCTGATCCAGAGGAACGCC
                  ************************************************************

HsCD00044509      ACTATCCCCACCAAGCAGACCCAGACTTTCACCACCTACTCGGACAACCAGCCTGGGGTC
NM_002155.3       ACTATCCCCACCAAGCAGACCCAGACTTTCACCACCTACTCGGACAACCAGCCTGGGGTC
                  ************************************************************

HsCD00044509      TTCATCCAGGTGTATGAGGGTGAGAGGGCCATGACCAAGGACAACAACCTGCTGGGGCGT
NM_002155.3       TTCATCCAGGTGTATGAGGGTGAGAGGGCCATGACCAAGGACAACAACCTGCTGGGGCGT
                  ************************************************************

HsCD00044509      TTTGAACTCAGTGGCATCCCTCCTGCCCCACGTGGAGTCCCCCAGATAGAGGTGACCTTT
NM_002155.3       TTTGAACTCAGTGGCATCCCTCCTGCCCCACGTGGAGTCCCCCAGATAGAGGTGACCTTT
                  ************************************************************

HsCD00044509      GACATTGATGCTAATGGCATCCTGAGCGTGACAGCCACTGACAGGAGCACAGGTAAGGCT
NM_002155.3       GACATTGATGCTAATGGCATCCTGAGCGTGACAGCCACTGACAGGAGCACAGGTAAGGCT
                  ************************************************************

HsCD00044509      AACAAGATCACCATCACCAATGACAAGGGCCGGCTGAGCAAGGAGGAGGTGGAGAGGATG
NM_002155.3       AACAAGATCACCATCACCAATGACAAGGGCCGGCTGAGCAAGGAGGAGGTGGAGAGGATG
                  ************************************************************

HsCD00044509      GTTCATGAAGCCGAGCAGTACAAGGCTGAGGATGAGGCCCAGAGGGACAGAGTGGCTGCC
NM_002155.3       GTTCATGAAGCCGAGCAGTACAAGGCTGAGGATGAGGCCCAGAGGGACAGAGTGGCTGCC
                  ************************************************************

HsCD00044509      AAAAACTCGCTGGAGGCCCATGTCTTCCATGTGAAAGGTTCTTTGCAAGAGGAAAGCCTT
NM_002155.3       AAAAACTCGCTGGAGGCCCATGTCTTCCATGTGAAAGGTTCTTTGCAAGAGGAAAGCCTT
                  ************************************************************

HsCD00044509      AGGGACAAGATTCCCGAAGAGGACAGGCGCAAAATGCAAGACAAGTGTCGGGAAGTCCTT
NM_002155.3       AGGGACAAGATTCCCGAAGAGGACAGGCGCAAAATGCAAGACAAGTGTCGGGAAGTCCTT
                  ************************************************************

HsCD00044509      GCCTGGCTGGAGCACAACCAGCTGGCAGAGAAGGAGGAGTATGAGCATCAGAAGAGGGAG
NM_002155.3       GCCTGGCTGGAGCACAACCAGCTGGCAGAGAAGGAGGAGTATGAGCATCAGAAGAGGGAG
                  ************************************************************

HsCD00044509      CTGGAGCAAATCTGTCGCCCCATCTTCTCCAGGCTCTATGGGGGGCCTGGTGTCCCTGGG
NM_002155.3       CTGGAGCAAATCTGTCGCCCCATCTTCTCCAGGCTCTATGGGGGGCCTGGTGTCCCTGGG
                  ************************************************************

HsCD00044509      GGCAGCAGTTGTGGCACTCAAGCCCGCCAGGGGGACCCCAGCACCGGCCCCATCATTGAG
NM_002155.3       GGCAGCAGTTGTGGCACTCAAGCCCGCCAGGGGGACCCCAGCACCGGCCCCATCATTGAG
                  ************************************************************

HsCD00044509      GAGGTTGATTAG
NM_002155.3       GAGGTTGATTGA
                  **********..

Pathways

Panther : Apoptosis signaling pathway
Panther : Parkinson disease
Reactome : Attenuation phase
Reactome : Cellular response to heat stress
Reactome : Cellular responses to stress
Reactome : HSF1 activation
Reactome : HSF1-dependent transactivation
Reactome : Regulation of HSF1-mediated heat shock response

Protein

UniProt : P17066
HPRD : 00775

Clone

Cloning Information : 186393
HIP Master Clone ID : 161337
Original Clone ID : FLH186393.01X

Organism


TAX_ID : 9606
Species Specific ID: 3310

Genome

Chromosome : 1
Map Location : 1q23
Ensembl : ENSG00000173110

Reagents

Labome : HSPA6-antibody