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KLHL6 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  KLHL6
Gene Name:  kelch-like family member 6
Sequence             
Original Clone ID: ccsbBroad304_12923
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00445093

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1831nts         Open reading frame : 1 to 1831
Reference Sequence Alignment

ATGGGTGATGTGGTCGAGAAGAGTTTGGAAGGGCCCCTGGCACCTTCTACAGATGAGCCC
TCCCAGAAAACAGGAGACTTGGTCGAGATCTTAAATGGGGAAAAGGTCAAATTTGACGAC
GCGGGACTCTCCTTAATTCTTCAGAATGGCCTGGAAACCCTGCGAATGGAAAACGCTCTG
ACAGATGTCATCTTGTGTGTGGACATTCAGGAATTCTCCTGCCACCGCGTGGTGCTTGCC
GCAGCCAGCAACTATTTCAGGGCCATGTTCTGCAACGATTTAAAGGAAAAGTATGAGAAA
AGGATCATTATTAAAGGGGTTGATGCTGAGACCATGCACACTCTGTTGGACTACACGTAC
ACCAGCAAGGCGCTGATCACCAAGCAGAATGTCCAGCGGGTCCTGGAGGCTGCTAACCTC
TTCCAGTTCCTGCGGATGGTGGATGCCTGTGCCAGCTTCCTCACTGAAGCCTTGAACCCA
GAAAACTGCGTTGGAATACTGAGGCTGGCTGACACACACTCGCTGGACAGTCTAAAGAAG
CAGGTTCAGAGTTACATCATTCAAAACTTTGTGCAGATTCTGAACTCTGAGGAGTTTCTT
GACCTGCCCGTGGACACTCTGCACCACATCTTGAAGAGTGATGACCTTTACGTGACCGAG
GAGGCTCAGGTGTTTGAGACCGTGATGAGCTGGGTCCGGCACAAGCCATCAGAACGACTC
TGCTTACTCCCCTATGTCCTCGAGAACGTGCGCTTACCGCTTCTGGACCCGTGGTACTTT
GTGGAGACGGTGGAAGCAGATCCTCTCATCAGGCAGTGCCCAGAGGTCTTCCCGCTGCTC
CAGGAAGCCAGGATGTACCACCTTTCTGGCAATGAGATCATTTCGGAACGCACCAAGCCC
AGGATGCATGAGTTCCAGTCTGAGGTGTTCATGATCATTGGCGGCTGCACGAAGGATGAA
CGGTTTGTGGCAGAGGTGACCTGCCTGGACCCCCTGAGGCGCAGCCGCCTGGAGGTGGCC
AAGCTCCCGCTAACAGAGCATGAGCTGGAGAGTGAGAATAAGAAGTGGGTGGAGTTTGCA
TGCGTGACATTGAAAAATGAGGTCTACATCTCAGGTGGCAAAGAAACACAGCATGATGTT
TGGAAATATAATTCTTCGATCAACAAGTGGATTCAGATTGAGTATTTAAACATAGGCCGC
TGGAGGCATAAGATGGTGGTGTTGGGTGGCAAGGTCTATGTGATCGGAGGCTTTGACGGC
TTACAGAGAATCAACAACGTGGAGACCTACGACCCCTTTCACAACTGCTGGTCAGAGGCC
GCACCCCTCCTTGTCCATGTCAGTTCCTTTGCAGCCACCAGCCATAAGAAGAAGCTGTAT
GTGATCGGGGGAGGGCCCAATGGGAAACTGGCCACAGACAAGACTCAGTGTTATGACCCT
TCCACCAACAAGTGGAGTTTGAAGGCGGCCATGCCCGTGGAGGCTAAATGCATCAATGCA
GTGAGTTTCCGGGACCGCATCTATGTCGTTGGTGGGGCCATGAGAGCGCTGTACGCCTAC
AGCCCGCTGGAAGACAGCTGGTGCCTGGTGACCCAGCTCAGCCACGAGCGGGCCAGCTGC
GGTATCGCGCCCTGCAACAACCGGCTCTACATCACCGGCGGGCGGGACGAGAAGAACGAG
GTTATCGCCACGGTGCTGTGCTGGGACCCCGAGGCCCAGAAACTGACAGAGGAGTGCGTC
CTGCCCCGGGGCGTGTCGCACCACGGCAGCGTCACCATCAGGAAGTCGTACACCCACATC
CGCAGGATCGTGCCCGGAGCAGTGTCTGTC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : KLHL6
Symbol Nomenclature : KLHL6
Designation : kelch-like 6|kelch-like protein 6|kelch-like protein KLHL6
Full Nomenclature : kelch-like family member 6
GENEID : 89857
GenBank Accession : EU176275
HGNC : 18653
MIM : 614214
Vega : OTTHUMG00000148673
Target GenBank: EU176275

 

Reference Sequence Alignment

  

HsCD00445093      ---------------------------------ATGGGTGATGTGGTCGAGAAGAGTTTG
NM_130446.2       ATGTTGATGGCAGGACAAAGGGGCGCCTGGACCATGGGTGATGTGGTCGAGAAGAGTTTG
                                                   ***************************

HsCD00445093      GAAGGGCCCCTGGCACCTTCTACAGATGAGCCCTCCCAGAAAACAGGAGACTTGGTCGAG
NM_130446.2       GAAGGGCCCCTGGCACCTTCTACAGATGAGCCCTCCCAGAAAACAGGAGACTTGGTCGAG
                  ************************************************************

HsCD00445093      ATCTTAAATGGGGAAAAGGTCAAATTTGACGACGCGGGACTCTCCTTAATTCTTCAGAAT
NM_130446.2       ATCTTAAATGGGGAAAAGGTCAAATTTGACGACGCGGGACTCTCCTTAATTCTTCAGAAT
                  ************************************************************

HsCD00445093      GGCCTGGAAACCCTGCGAATGGAAAACGCTCTGACAGATGTCATCTTGTGTGTGGACATT
NM_130446.2       GGCCTGGAAACCCTGCGAATGGAAAACGCTCTGACAGATGTCATCTTGTGTGTGGACATT
                  ************************************************************

HsCD00445093      CAGGAATTCTCCTGCCACCGCGTGGTGCTTGCCGCAGCCAGCAACTATTTCAGGGCCATG
NM_130446.2       CAGGAATTCTCCTGCCACCGCGTGGTGCTTGCCGCAGCCAGCAACTATTTCAGGGCCATG
                  ************************************************************

HsCD00445093      TTCTGCAACGATTTAAAGGAAAAGTATGAGAAAAGGATCATTATTAAAGGGGTTGATGCT
NM_130446.2       TTCTGCAACGATTTAAAGGAAAAGTATGAGAAAAGGATCATTATTAAAGGGGTTGATGCT
                  ************************************************************

HsCD00445093      GAGACCATGCACACTCTGTTGGACTACACGTACACCAGCAAGGCGCTGATCACCAAGCAG
NM_130446.2       GAGACCATGCACACTCTGTTGGACTACACGTACACCAGCAAGGCGCTGATCACCAAGCAG
                  ************************************************************

HsCD00445093      AATGTCCAGCGGGTCCTGGAGGCTGCTAACCTCTTCCAGTTCCTGCGGATGGTGGATGCC
NM_130446.2       AATGTCCAGCGGGTCCTGGAGGCTGCTAACCTCTTCCAGTTCCTGCGGATGGTGGATGCC
                  ************************************************************

HsCD00445093      TGTGCCAGCTTCCTCACTGAAGCCTTGAACCCAGAAAACTGCGTTGGAATACTGAGGCTG
NM_130446.2       TGTGCCAGCTTCCTCACTGAAGCCTTGAACCCAGAAAACTGCGTTGGAATACTGAGGCTG
                  ************************************************************

HsCD00445093      GCTGACACACACTCGCTGGACAGTCTAAAGAAGCAGGTTCAGAGTTACATCATTCAAAAC
NM_130446.2       GCTGACACACACTCGCTGGACAGTCTAAAGAAGCAGGTTCAGAGTTACATCATTCAAAAC
                  ************************************************************

HsCD00445093      TTTGTGCAGATTCTGAACTCTGAGGAGTTTCTTGACCTGCCCGTGGACACTCTGCACCAC
NM_130446.2       TTTGTGCAGATTCTGAACTCTGAGGAGTTTCTTGACCTGCCCGTGGACACTCTGCACCAC
                  ************************************************************

HsCD00445093      ATCTTGAAGAGTGATGACCTTTACGTGACCGAGGAGGCTCAGGTGTTTGAGACCGTGATG
NM_130446.2       ATCTTGAAGAGTGATGACCTTTACGTGACCGAGGAGGCTCAGGTGTTTGAGACCGTGATG
                  ************************************************************

HsCD00445093      AGCTGGGTCCGGCACAAGCCATCAGAACGACTCTGCTTACTCCCCTATGTCCTCGAGAAC
NM_130446.2       AGCTGGGTCCGGCACAAGCCATCAGAACGACTCTGCTTACTCCCCTATGTCCTCGAGAAC
                  ************************************************************

HsCD00445093      GTGCGCTTACCGCTTCTGGACCCGTGGTACTTTGTGGAGACGGTGGAAGCAGATCCTCTC
NM_130446.2       GTGCGCTTACCGCTTCTGGACCCGTGGTACTTTGTGGAGACGGTGGAAGCAGATCCTCTC
                  ************************************************************

HsCD00445093      ATCAGGCAGTGCCCAGAGGTCTTCCCGCTGCTCCAGGAAGCCAGGATGTACCACCTTTCT
NM_130446.2       ATCAGGCAGTGCCCAGAGGTCTTCCCGCTGCTCCAGGAAGCCAGGATGTACCACCTTTCT
                  ************************************************************

HsCD00445093      GGCAATGAGATCATTTCGGAACGCACCAAGCCCAGGATGCATGAGTTCCAGTCTGAGGTG
NM_130446.2       GGCAATGAGATCATTTCGGAACGCACCAAGCCCAGGATGCATGAGTTCCAGTCTGAGGTG
                  ************************************************************

HsCD00445093      TTCATGATCATTGGCGGCTGCACGAAGGATGAACGGTTTGTGGCAGAGGTGACCTGCCTG
NM_130446.2       TTCATGATCATTGGCGGCTGCACGAAGGATGAACGGTTTGTGGCAGAGGTGACCTGCCTG
                  ************************************************************

HsCD00445093      GACCCCCTGAGGCGCAGCCGCCTGGAGGTGGCCAAGCTCCCGCTAACAGAGCATGAGCTG
NM_130446.2       GACCCCCTGAGGCGCAGCCGCCTGGAGGTGGCCAAGCTCCCGCTAACAGAGCATGAGCTG
                  ************************************************************

HsCD00445093      GAGAGTGAGAATAAGAAGTGGGTGGAGTTTGCATGCGTGACATTGAAAAATGAGGTCTAC
NM_130446.2       GAGAGTGAGAATAAGAAGTGGGTGGAGTTTGCATGCGTGACATTGAAAAATGAGGTCTAC
                  ************************************************************

HsCD00445093      ATCTCAGGTGGCAAAGAAACACAGCATGATGTTTGGAAATATAATTCTTCGATCAACAAG
NM_130446.2       ATCTCAGGTGGCAAAGAAACACAGCATGATGTTTGGAAATATAATTCTTCGATCAACAAG
                  ************************************************************

HsCD00445093      TGGATTCAGATTGAGTATTTAAACATAGGCCGCTGGAGGCATAAGATGGTGGTGTTGGGT
NM_130446.2       TGGATTCAGATTGAGTATTTAAACATAGGCCGCTGGAGGCATAAGATGGTGGTGTTGGGT
                  ************************************************************

HsCD00445093      GGCAAGGTCTATGTGATCGGAGGCTTTGACGGCTTACAGAGAATCAACAACGTGGAGACC
NM_130446.2       GGCAAGGTCTATGTGATCGGAGGCTTTGACGGCTTACAGAGAATCAACAATGTGGAGACC
                  ************************************************** *********

HsCD00445093      TACGACCCCTTTCACAACTGCTGGTCAGAGGCCGCACCCCTCCTTGTCCATGTCAGTTCC
NM_130446.2       TACGACCCCTTTCACAACTGCTGGTCAGAGGCCGCACCCCTCCTTGTCCATGTCAGTTCC
                  ************************************************************

HsCD00445093      TTTGCAGCCACCAGCCATAAGAAGAAGCTGTATGTGATCGGGGGAGGGCCCAATGGGAAA
NM_130446.2       TTTGCAGCCACCAGCCATAAGAAGAAGCTGTATGTGATCGGGGGAGGGCCCAATGGGAAA
                  ************************************************************

HsCD00445093      CTGGCCACAGACAAGACTCAGTGTTATGACCCTTCCACCAACAAGTGGAGTTTGAAGGCG
NM_130446.2       CTGGCCACAGACAAGACTCAGTGTTATGACCCTTCCACCAACAAGTGGAGTTTGAAGGCG
                  ************************************************************

HsCD00445093      GCCATGCCCGTGGAGGCTAAATGCATCAATGCAGTGAGTTTCCGGGACCGCATCTATGTC
NM_130446.2       GCCATGCCCGTGGAGGCTAAATGCATCAATGCAGTGAGTTTCCGGGACCGCATCTATGTC
                  ************************************************************

HsCD00445093      GTTGGTGGGGCCATGAGAGCGCTGTACGCCTACAGCCCGCTGGAAGACAGCTGGTGCCTG
NM_130446.2       GTTGGTGGGGCCATGAGAGCGCTGTACGCCTACAGCCCGCTGGAAGACAGCTGGTGCCTG
                  ************************************************************

HsCD00445093      GTGACCCAGCTCAGCCACGAGCGGGCCAGCTGCGGTATCGCGCCCTGCAACAACCGGCTC
NM_130446.2       GTGACCCAGCTCAGCCACGAGCGGGCCAGCTGCGGTATCGCGCCCTGCAACAACCGGCTC
                  ************************************************************

HsCD00445093      TACATCACCGGCGGGCGGGACGAGAAGAACGAGGTTATCGCCACGGTGCTGTGCTGGGAC
NM_130446.2       TACATCACCGGCGGGCGGGACGAGAAGAACGAGGTTATCGCCACGGTGCTGTGCTGGGAC
                  ************************************************************

HsCD00445093      CCCGAGGCCCAGAAACTGACAGAGGAGTGCGTCCTGCCCCGGGGCGTGTCGCACCACGGC
NM_130446.2       CCCGAGGCCCAGAAACTGACAGAGGAGTGCGTCCTGCCCCGGGGCGTGTCGCACCACGGC
                  ************************************************************

HsCD00445093      AGCGTCACCATCAGGAAGTCGTACACCCACATCCGCAGGATCGTGCCCGGAGCAGTGTCT
NM_130446.2       AGCGTCACCATCAGGAAGTCGTACACCCACATCCGCAGGATCGTGCCCGGAGCAGTGTCT
                  ************************************************************

HsCD00445093      GTC---
NM_130446.2       GTCTGA
                  ***   

Protein

SMART domain : Kelch :
SMART domain : BACK : BTB And C-terminal Kelch
SMART domain : BTB : Broad-Complex, Tramtrack and Bric a brac
HPRD : 10035

Clone

Original Clone ID : ccsbBroad304_12923

Organism


TAX_ID : 9606
Species Specific ID: 89857

Genome

Chromosome : 3
Map Location : 3q27.3
Ensembl : ENSG00000172578

Reagents

Labome : KLHL6-antibody