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CYP4F11 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  CYP4F11
Gene Name:  cytochrome P450, family 4, subfamily F, polypeptide 11
Sequence             
Original Clone ID: ccsbBroad304_08771
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00445160

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1573nts         Open reading frame : 1 to 1573
Reference Sequence Alignment

ATGCCGCAGCTGAGCCTGTCCTGGCTGGGCCTCGGGCCCGTGGCAGCATCCCCGTGGCTG
CTTCTGCTGCTGGTTGGGGGCTCCTGGCTCCTGGCCCGCGTCCTGGCCTGGACCTACACC
TTCTATGACAACTGCCGCCGCCTCCAGTGTTTTCCTCAACCCCCGAAACAGAACTGGTTT
TGGGGACACCAGGGCCTGGTCACTCCCACGGAAGAGGGCATGAAGACATTGACCCAGCTG
GTGACCACATATCCCCAGGGCTTTAAGTTGTGGCTGGGTCCTACCTTCCCCCTCCTCATT
TTATGCCACCCTGACATCATCCGGCCTATCACCAGTGCCTCAGCTGCTGTCGCACCCAAG
GATATGATTTTCTATGGCTTCCTGAAGCCCTGGCTGGGGGATGGGCTCCTGCTGAGTGGT
GGTGACAAGTGGAGCCGCCACCGTCGGATGTTGACGCCTGCCTTCCATTTCAACATCTTG
AAGCCTTATATGAAGATTTTCAACAAGAGTGTGAACATCATGCACGACAAGTGGCAGCGC
CTGGCCTCAGAGGGCAGCGCCAGACTGGACATGTTTGAACACATCAGCCTCATGACCTTG
GACAGTCTGCAGAAATGTGTCTTCAGCTTTGAAAGCAATTGTCAGGAGAAGCCCAGTGAA
TATATTGCCGCCATCTTGGAGCTCAGTGCCTTTGTAGAAAAGAGAAACCAGCAGATTCTC
TTGCACACGGACTTCCTGTATTATCTCACTCCTGATGGGCAGCGCTTCCGCAGGGCCTGC
CACCTGGTGCACGACTTCACAGATGCCGTCATCCAGGAGCGGCGCCGCACCCTCCCCACT
CAGGGTATTGATGATTTCCTCAAGAACAAGGCAAAGTCCAAGACTTTAGACTTCATTGAT
GTGCTTCTGCTGAGCAAGGATGAAGATGGGAAGGAATTGTCTGATGAGGACATAAGAGCA
GAAGCTGACACCTTCATGTTTGAGGGCCATGACACTACAGCCAGTGGTCTCTCCTGGGTC
CTATACCACCTTGCAAAGCACCCAGAATACCAGGAACAGTGCCGGCAAGAAGTGCAAGAG
CTTCTGAAGGACCGTGAACCTATAGAGATTGAATGGGACGACCTGGCCCAGCTGCCCTTC
CTGACCATGTGCATTAAGGAGAGCCTGCGGTTGCATCCCCCAGTCCCGGTCATCTCCCGA
TGTTGCACGCAGGACTTTGTGCTCCCAGACGGCCGCGTCATCCCCAAAGGCATTGTCTGC
CTCATCAATATTATCGGGATCCATTACAACCCAACTGTGTGGCCAGACCCTGAGGTCTAC
GACCCCTTCCGTTTCAACCAAGAGAACATCAAGGAGAGGTCACCTCTGGCTTTTATTCCC
TTCTCGGCAGGGCCCAGAAACTGCATCGGGCAGGCGTTCGCCATGGCTGAGATGAAGGTG
GTCCTGGCGCTCACGCTGCTGCACTTCCGCATCCTGCCGACCCACACTGAACCCCGCAGG
AAACCCGAGCTGATATTGCGCGCAGAGGGTGGACTTTGGCTGCGGGTGGAGCCCCTGGGT
GCGAACTCACAG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CYP4F11
Symbol Nomenclature : CYP4F11
SYNONYM : CYPIVF11
Designation : cytochrome P450 4F11|cytochrome P450, subfamily IVF, polypeptide 11
Full Nomenclature : cytochrome P450, family 4, subfamily F, polypeptide 11
GENEID : 57834
GenBank Accession : DQ894650
HGNC : 13265
MIM : 611517
Vega : OTTHUMG00000182294
Target GenBank: DQ894650

 

Reference Sequence Alignment

  

HsCD00445160        ATGCCGCAGCTGAGCCTGTCCTGGCTGGGCCTCGGGCCCGTGGCAGCATCCCCGTGGCTG
NM_001128932.1      ATGCCGCAGCTGAGCCTGTCCTGGCTGGGCCTCGGGCCCGTGGCAGCATCCCCGTGGCTG
NM_021187.3         ATGCCGCAGCTGAGCCTGTCCTGGCTGGGCCTCGGGCCCGTGGCAGCATCCCCGTGGCTG
                    ************************************************************

HsCD00445160        CTTCTGCTGCTGGTTGGGGGCTCCTGGCTCCTGGCCCGCGTCCTGGCCTGGACCTACACC
NM_001128932.1      CTTCTGCTGCTGGTTGGGGGCTCCTGGCTCCTGGCCCGCGTCCTGGCCTGGACCTACACC
NM_021187.3         CTTCTGCTGCTGGTTGGGGGCTCCTGGCTCCTGGCCCGCGTCCTGGCCTGGACCTACACC
                    ************************************************************

HsCD00445160        TTCTATGACAACTGCCGCCGCCTCCAGTGTTTTCCTCAACCCCCGAAACAGAACTGGTTT
NM_001128932.1      TTCTATGACAACTGCCGCCGCCTCCAGTGTTTTCCTCAACCCCCGAAACAGAACTGGTTT
NM_021187.3         TTCTATGACAACTGCCGCCGCCTCCAGTGTTTTCCTCAACCCCCGAAACAGAACTGGTTT
                    ************************************************************

HsCD00445160        TGGGGACACCAGGGCCTGGTCACTCCCACGGAAGAGGGCATGAAGACATTGACCCAGCTG
NM_001128932.1      TGGGGACACCAGGGCCTGGTCACTCCCACGGAAGAGGGCATGAAGACATTGACCCAGCTG
NM_021187.3         TGGGGACACCAGGGCCTGGTCACTCCCACGGAAGAGGGCATGAAGACATTGACCCAGCTG
                    ************************************************************

HsCD00445160        GTGACCACATATCCCCAGGGCTTTAAGTTGTGGCTGGGTCCTACCTTCCCCCTCCTCATT
NM_001128932.1      GTGACCACATATCCCCAGGGCTTTAAGTTGTGGCTGGGTCCTACCTTCCCCCTCCTCATT
NM_021187.3         GTGACCACATATCCCCAGGGCTTTAAGTTGTGGCTGGGTCCTACCTTCCCCCTCCTCATT
                    ************************************************************

HsCD00445160        TTATGCCACCCTGACATCATCCGGCCTATCACCAGTGCCTCAGCTGCTGTCGCACCCAAG
NM_001128932.1      TTATGCCACCCTGACATTATCCGGCCTATCACCAGTGCCTCAGCTGCTGTCGCACCCAAG
NM_021187.3         TTATGCCACCCTGACATTATCCGGCCTATCACCAGTGCCTCAGCTGCTGTCGCACCCAAG
                    ***************** ******************************************

HsCD00445160        GATATGATTTTCTATGGCTTCCTGAAGCCCTGGCTGGGGGATGGGCTCCTGCTGAGTGGT
NM_001128932.1      GATATGATTTTCTATGGCTTCCTGAAGCCCTGGCTGGGGGATGGGCTCCTGCTGAGTGGT
NM_021187.3         GATATGATTTTCTATGGCTTCCTGAAGCCCTGGCTGGGGGATGGGCTCCTGCTGAGTGGT
                    ************************************************************

HsCD00445160        GGTGACAAGTGGAGCCGCCACCGTCGGATGTTGACGCCTGCCTTCCATTTCAACATCTTG
NM_001128932.1      GGTGACAAGTGGAGCCGCCACCGTCGGATGTTGACGCCTGCCTTCCATTTCAACATCTTG
NM_021187.3         GGTGACAAGTGGAGCCGCCACCGTCGGATGTTGACGCCTGCCTTCCATTTCAACATCTTG
                    ************************************************************

HsCD00445160        AAGCCTTATATGAAGATTTTCAACAAGAGTGTGAACATCATGCACGACAAGTGGCAGCGC
NM_001128932.1      AAGCCTTATATGAAGATTTTCAACAAGAGTGTGAACATCATGCACGACAAGTGGCAGCGC
NM_021187.3         AAGCCTTATATGAAGATTTTCAACAAGAGTGTGAACATCATGCACGACAAGTGGCAGCGC
                    ************************************************************

HsCD00445160        CTGGCCTCAGAGGGCAGCGCCAGACTGGACATGTTTGAACACATCAGCCTCATGACCTTG
NM_001128932.1      CTGGCCTCAGAGGGCAGCGCCAGACTGGACATGTTTGAACACATCAGCCTCATGACCTTG
NM_021187.3         CTGGCCTCAGAGGGCAGCGCCAGACTGGACATGTTTGAACACATCAGCCTCATGACCTTG
                    ************************************************************

HsCD00445160        GACAGTCTGCAGAAATGTGTCTTCAGCTTTGAAAGCAATTGTCAGGAGAAGCCCAGTGAA
NM_001128932.1      GACAGTCTGCAGAAATGTGTCTTCAGCTTTGAAAGCAATTGTCAGGAGAAGCCCAGTGAA
NM_021187.3         GACAGTCTGCAGAAATGTGTCTTCAGCTTTGAAAGCAATTGTCAGGAGAAGCCCAGTGAA
                    ************************************************************

HsCD00445160        TATATTGCCGCCATCTTGGAGCTCAGTGCCTTTGTAGAAAAGAGAAACCAGCAGATTCTC
NM_001128932.1      TATATTGCCGCCATCTTGGAGCTCAGTGCCTTTGTAGAAAAGAGAAACCAGCAGATTCTC
NM_021187.3         TATATTGCCGCCATCTTGGAGCTCAGTGCCTTTGTAGAAAAGAGAAACCAGCAGATTCTC
                    ************************************************************

HsCD00445160        TTGCACACGGACTTCCTGTATTATCTCACTCCTGATGGGCAGCGCTTCCGCAGGGCCTGC
NM_001128932.1      TTGCACACGGACTTCCTGTATTATCTCACTCCTGATGGGCAGCGCTTCCGCAGGGCCTGC
NM_021187.3         TTGCACACGGACTTCCTGTATTATCTCACTCCTGATGGGCAGCGCTTCCGCAGGGCCTGC
                    ************************************************************

HsCD00445160        CACCTGGTGCACGACTTCACAGATGCCGTCATCCAGGAGCGGCGCCGCACCCTCCCCACT
NM_001128932.1      CACCTGGTGCACGACTTCACAGATGCCGTCATCCAGGAGCGGCGCTGCACCCTCCCCACT
NM_021187.3         CACCTGGTGCACGACTTCACAGATGCCGTCATCCAGGAGCGGCGCTGCACCCTCCCCACT
                    ********************************************* **************

HsCD00445160        CAGGGTATTGATGATTTCCTCAAGAACAAGGCAAAGTCCAAGACTTTAGACTTCATTGAT
NM_001128932.1      CAGGGTATTGATGATTTCCTCAAGAACAAGGCAAAGTCCAAGACTTTAGACTTCATTGAT
NM_021187.3         CAGGGTATTGATGATTTCCTCAAGAACAAGGCAAAGTCCAAGACTTTAGACTTCATTGAT
                    ************************************************************

HsCD00445160        GTGCTTCTGCTGAGCAAGGATGAAGATGGGAAGGAATTGTCTGATGAGGACATAAGAGCA
NM_001128932.1      GTGCTTCTGCTGAGCAAGGATGAAGATGGGAAGGAATTGTCTGATGAGGACATAAGAGCA
NM_021187.3         GTGCTTCTGCTGAGCAAGGATGAAGATGGGAAGGAATTGTCTGATGAGGACATAAGAGCA
                    ************************************************************

HsCD00445160        GAAGCTGACACCTTCATGTTTGAGGGCCATGACACTACAGCCAGTGGTCTCTCCTGGGTC
NM_001128932.1      GAAGCTGACACCTTCATGTTTGAGGGCCATGACACTACAGCCAGTGGTCTCTCCTGGGTC
NM_021187.3         GAAGCTGACACCTTCATGTTTGAGGGCCATGACACTACAGCCAGTGGTCTCTCCTGGGTC
                    ************************************************************

HsCD00445160        CTATACCACCTTGCAAAGCACCCAGAATACCAGGAACAGTGCCGGCAAGAAGTGCAAGAG
NM_001128932.1      CTATACCACCTTGCAAAGCACCCAGAATACCAGGAACAGTGCCGGCAAGAAGTGCAAGAG
NM_021187.3         CTATACCACCTTGCAAAGCACCCAGAATACCAGGAACAGTGCCGGCAAGAAGTGCAAGAG
                    ************************************************************

HsCD00445160        CTTCTGAAGGACCGTGAACCTATAGAGATTGAATGGGACGACCTGGCCCAGCTGCCCTTC
NM_001128932.1      CTTCTGAAGGACCGTGAACCTATAGAGATTGAATGGGACGACCTGGCCCAGCTGCCCTTC
NM_021187.3         CTTCTGAAGGACCGTGAACCTATAGAGATTGAATGGGACGACCTGGCCCAGCTGCCCTTC
                    ************************************************************

HsCD00445160        CTGACCATGTGCATTAAGGAGAGCCTGCGGTTGCATCCCCCAGTCCCGGTCATCTCCCGA
NM_001128932.1      CTGACCATGTGCATTAAGGAGAGCCTGCGGTTGCATCCCCCAGTCCCGGTCATCTCCCGA
NM_021187.3         CTGACCATGTGCATTAAGGAGAGCCTGCGGTTGCATCCCCCAGTCCCGGTCATCTCCCGA
                    ************************************************************

HsCD00445160        TGTTGCACGCAGGACTTTGTGCTCCCAGACGGCCGCGTCATCCCCAAAGGCATTGTCTGC
NM_001128932.1      TGTTGCACGCAGGACTTTGTGCTCCCAGACGGCCGCGTCATCCCCAAAGGCATTGTCTGC
NM_021187.3         TGTTGCACGCAGGACTTTGTGCTCCCAGACGGCCGCGTCATCCCCAAAGGCATTGTCTGC
                    ************************************************************

HsCD00445160        CTCATCAATATTATCGGGATCCATTACAACCCAACTGTGTGGCCAGACCCTGAGGTCTAC
NM_001128932.1      CTCATCAATATTATCGGGATCCATTACAACCCAACTGTGTGGCCAGACCCTGAGGTCTAC
NM_021187.3         CTCATCAATATTATCGGGATCCATTACAACCCAACTGTGTGGCCAGACCCTGAGGTCTAC
                    ************************************************************

HsCD00445160        GACCCCTTCCGTTTCAACCAAGAGAACATCAAGGAGAGGTCACCTCTGGCTTTTATTCCC
NM_001128932.1      GACCCCTTCCGTTTCGACCAAGAGAACATCAAGGAGAGGTCACCTCTGGCTTTTATTCCC
NM_021187.3         GACCCCTTCCGTTTCGACCAAGAGAACATCAAGGAGAGGTCACCTCTGGCTTTTATTCCC
                    ***************.********************************************

HsCD00445160        TTCTCGGCAGGGCCCAGAAACTGCATCGGGCAGGCGTTCGCCATGGCTGAGATGAAGGTG
NM_001128932.1      TTCTCGGCAGGGCCCAGAAACTGCATCGGGCAGGCGTTCGCCATGGCTGAGATGAAGGTG
NM_021187.3         TTCTCGGCAGGGCCCAGAAACTGCATCGGGCAGGCGTTCGCCATGGCTGAGATGAAGGTG
                    ************************************************************

HsCD00445160        GTCCTGGCGCTCACGCTGCTGCACTTCCGCATCCTGCCGACCCACACTGAACCCCGCAGG
NM_001128932.1      GTCCTGGCGCTCACCCTGCTGCACTTCCGCATCCTGCCGACCCACACTGAACCCCGCAGG
NM_021187.3         GTCCTGGCGCTCACCCTGCTGCACTTCCGCATCCTGCCGACCCACACTGAACCCCGCAGG
                    ************** *********************************************

HsCD00445160        AAACCCGAGCTGATATTGCGCGCAGAGGGTGGACTTTGGCTGCGGGTGGAGCCCCTGGGT
NM_001128932.1      AAACCCGAGCTGATATTGCGCGCAGAGGGTGGACTTTGGCTGCGGGTGGAGCCCCTGGGT
NM_021187.3         AAACCCGAGCTGATATTGCGCGCAGAGGGTGGACTTTGGCTGCGGGTGGAGCCCCTGGGT
                    ************************************************************

HsCD00445160        GCGAACTCACAG---
NM_001128932.1      GCGAACTCACAGTGA
NM_021187.3         GCGAACTCACAGTGA
                    ************   

Pathways

Reactome : Arachidonic acid metabolism
Reactome : Biological oxidations
Reactome : Cytochrome P450 - arranged by substrate type
Reactome : Eicosanoids
Reactome : Fatty acids
Reactome : Metabolism
Reactome : Metabolism of lipids and lipoproteins
Reactome : Miscellaneous substrates
Reactome : Phase 1 - Functionalization of compounds
Reactome : Synthesis of Leukotrienes (LT) and Eoxins (EX)
WikiPathway : cytochrome P450
WikiPathway : metapathway biotransformation

Protein

HPRD : 07101

Clone

Original Clone ID : ccsbBroad304_08771

Organism


TAX_ID : 9606
Species Specific ID: 57834

Genome

Chromosome : 19
Map Location : 19p13.1
Ensembl : ENSG00000171903

Reagents

Labome : CYP4F11-antibody